Rh6DG431500

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011405
Physical Location & Seq
Forward (+)
61076580 .. 61080670
4091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG431500.1

Sequence Viewer

Length: 789 bp
ATGGCTCAACTGGGAAGACTGCTGGTAAGGCAATGCCCGGCGTTGCAGCACCACTGCTGCTCCTGCGTCACACTCAATCATCTCTTCTTCTCAACCTCACTTCCACTAAGCACAAACCCTAATTCCCGCCGCTCTTCTTCCCCGGTAATCTACTTAGCTACTCGCGGCGTCCATAAACTAGTCCGCGGACTCCGACCGCGCGACGCTCCTCCGGCACCGTCCGATCTCGACGGTAACAGCAGCGGTTCCGATTCGGATTCGGTTACATATAAGAGCCGCAACGCCTTGAAACGCGACGCTCGGCGCGCCGTCCGGTGGGCTATGGACCTCGCGTCCTTCTCTACACCTCAGCTCAAATTGATTATCAGAGTGGCTTCTCTGGACCAAGATGTATTGGATGCTGTTATGTTAGTGAAGAGATTTGGGAATGATGTGCGAGAAGGAAAGCGAAGGCAGTTCAATTATATTGCAAAATTGCTACGAGATGCGGACCCTGAGTTGATGGATGCTTTGATTCGCGCTACAAAAGACTGCGACCAAAAGAAGTTGCAAGATTTATGTGGTTCGGAGGCGTTGAGCATTGACGATGAGGAAGAAGAAGAAGAAGAAGAAGAAGCTGAAGAATCTGATAACGAGGAAGAAGAGGGTTCTCACATTGATGTAGCAACCGGATGGGTTGAGGGTATGATCAATAAGGACGCTAATATCACCAATGAAGTTTATTCAATTTCAAATGTTGAGTTTGACCGTCAGGTGAGTGGAATGGCATCTCTACAACCTTGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

262

Amino Acids

29.22

Weight (kDa)

4.96

Isoelectric Point (pI)

66.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 90 - 243 6.6e-20 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 132
AccII CGCG 8 cut(s) 165, 186, 199, 201, 294, 306, 332, 519
AciI CCGC 9 cut(s) 127, 130, 165, 184, 186, 197, 243, 277, 488
AcuI CTGAAG 1 cut(s) 639
AcyI GRCGYC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 315
AgsI TTSAA 4 cut(s) 289, 460, 726, 732
AhdI GACNNNNNGTC 1 cut(s) 331
AhlI ACTAGT 1 cut(s) 178
AluBI AGCT 3 cut(s) 158, 352, 617
AluI AGCT 3 cut(s) 158, 352, 617
ApeKI GCWGC 3 cut(s) 46, 57, 240
AscI GGCGCGCC 1 cut(s) 304
AspLEI GCGC 4 cut(s) 201, 306, 308, 521
AspS9I GGNCC 3 cut(s) 325, 382, 490
AsuC2I CCSGG 2 cut(s) 38, 143
AsuHPI GGTGA 2 cut(s) 700, 766
AvaII GGWCC 3 cut(s) 325, 382, 490
BanI GGYRCC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 44, 58, 252
BccI CCATC 2 cut(s) 496, 666
BceAI ACGGC 1 cut(s) 293
BclI TGATCA 1 cut(s) 687
BcnI CCSGG 2 cut(s) 38, 143
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BisI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 277
BlsI GCNGC 6 cut(s) 48, 59, 131, 167, 242, 278
Bme1390I CCNGG 2 cut(s) 38, 143
Bme18I GGWCC 3 cut(s) 325, 382, 490
BmeRI GACNNNNNGTC 1 cut(s) 331
BmgT120I GGNCC 3 cut(s) 325, 382, 490
BmiI GGNNCC 3 cut(s) 216, 247, 492
BmrFI CCNGG 2 cut(s) 38, 143
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 4 cut(s) 388, 475, 496, 776
BmuI ACTGGG 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 348
BpuMI CCSGG 2 cut(s) 38, 143
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 141, 184
BsaWI WCCGGW 2 cut(s) 312, 668
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 315
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 38
BseDI CCNNGG 2 cut(s) 141, 184
BseGI GGATG 3 cut(s) 403, 511, 677
BseLI CCNNNNNNNGG 1 cut(s) 315
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 2 cut(s) 362, 486
BseNI ACTGG 1 cut(s) 15
BsePI GCGCGC 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 3 cut(s) 44, 58, 252
Bsh1236I CGCG 8 cut(s) 165, 186, 199, 201, 294, 306, 332, 519
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 197
BsiSI CCGG 5 cut(s) 38, 143, 212, 313, 669
BslI CCNNNNNNNGG 1 cut(s) 315
Bsp143I GATC 2 cut(s) 223, 687
BspACI CCGC 9 cut(s) 127, 130, 165, 184, 186, 197, 243, 277, 488
BspCNI CTCAG 2 cut(s) 361, 487
BspFNI CGCG 8 cut(s) 165, 186, 199, 201, 294, 306, 332, 519
BspLI GGNNCC 3 cut(s) 216, 247, 492
BspQI GCTCTTC 1 cut(s) 139
BspT107I GGYRCC 1 cut(s) 214
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 141, 184
BssHII GCGCGC 1 cut(s) 304
BssMI GATC 2 cut(s) 223, 687
BssNI GRCGYC 1 cut(s) 168
Bst4CI ACNGT 3 cut(s) 219, 233, 749
Bst6I CTCTTC 4 cut(s) 89, 139, 410, 636
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 4 cut(s) 107, 154, 348, 495
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 3 cut(s) 403, 511, 677
BstFNI CGCG 8 cut(s) 165, 186, 199, 201, 294, 306, 332, 519
BstHHI GCGC 4 cut(s) 201, 306, 308, 521
BstKTI GATC 2 cut(s) 226, 690
BstMBI GATC 2 cut(s) 223, 687
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 305
BstSCI CCNGG 2 cut(s) 36, 141
BstUI CGCG 8 cut(s) 165, 186, 199, 201, 294, 306, 332, 519
BstV1I GCAGC 3 cut(s) 44, 58, 252
BstV2I GAAGAC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 3 cut(s) 403, 511, 677
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 306
CfoI GCGC 4 cut(s) 201, 306, 308, 521
Cfr13I GGNCC 3 cut(s) 325, 382, 490
Cfr42I CCGCGG 1 cut(s) 187
CseI GACGC 6 cut(s) 55, 157, 212, 305, 321, 707
CviJI RGCY 7 cut(s) 5, 158, 276, 320, 352, 374, 617
CviKI_1 RGCY 7 cut(s) 5, 158, 276, 320, 352, 374, 617
DdeI CTNAG 4 cut(s) 107, 154, 348, 495
DpnI GATC 2 cut(s) 225, 689
DpnII GATC 2 cut(s) 223, 687
DriI GACNNNNNGTC 1 cut(s) 331
Eam1104I CTCTTC 4 cut(s) 89, 139, 410, 636
Eam1105I GACNNNNNGTC 1 cut(s) 331
EarI CTCTTC 4 cut(s) 89, 139, 410, 636
Eco47I GGWCC 3 cut(s) 325, 382, 490
Eco57I CTGAAG 1 cut(s) 639
FaiI YATR 8 cut(s) 174, 268, 270, 323, 407, 465, 559, 686
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 687
Fnu4HI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 277
FokI GGATG 3 cut(s) 410, 518, 684
Fsp4HI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 277
FspBI CTAG 1 cut(s) 179
GlaI GCGC 4 cut(s) 200, 305, 307, 520
GluI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 277
HapII CCGG 5 cut(s) 38, 143, 212, 313, 669
HgaI GACGC 6 cut(s) 55, 157, 212, 305, 321, 707
HhaI GCGC 4 cut(s) 201, 306, 308, 521
Hin1I GRCGYC 1 cut(s) 168
Hin6I GCGC 4 cut(s) 199, 304, 306, 519
HinP1I GCGC 4 cut(s) 199, 304, 306, 519
HinfI GANTC 5 cut(s) 189, 251, 257, 514, 623
HpaII CCGG 5 cut(s) 38, 143, 212, 313, 669
HphI GGTGA 2 cut(s) 700, 766
Hpy188I TCNGA 7 cut(s) 194, 223, 250, 256, 368, 568, 628
Hpy188III TCNNGA 2 cut(s) 227, 380
Hpy99I CGWCG 3 cut(s) 206, 233, 299
HpyAV CCTTC 3 cut(s) 346, 434, 444
HpyCH4III ACNGT 3 cut(s) 219, 233, 749
HpyCH4V TGCA 3 cut(s) 46, 470, 550
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 305
HpyF3I CTNAG 4 cut(s) 107, 154, 348, 495
Hsp92I GRCGYC 1 cut(s) 168
HspAI GCGC 4 cut(s) 199, 304, 306, 519
Ksp22I TGATCA 1 cut(s) 687
KspI CCGCGG 1 cut(s) 187
Kzo9I GATC 2 cut(s) 223, 687
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 2 cut(s) 65, 211
Lsp1109I GCAGC 3 cut(s) 44, 58, 252
LweI GCATC 4 cut(s) 388, 475, 496, 776
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 3 cut(s) 67, 233, 262
MalI GATC 2 cut(s) 225, 689
MbiI CCGCTC 1 cut(s) 132
MboI GATC 2 cut(s) 223, 687
MluCI AATT 5 cut(s) 121, 356, 460, 473, 726
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 9 cut(s) 106, 219, 338, 357, 562, 583, 628, 637, 673
MseI TTAA 1 cut(s) 787
MslI CAYNNNNRTG 1 cut(s) 657
MspA1I CMGCKG 2 cut(s) 186, 243
MspI CCGG 5 cut(s) 38, 143, 212, 313, 669
MspR9I CCNGG 2 cut(s) 38, 143
MvnI CGCG 8 cut(s) 165, 186, 199, 201, 294, 306, 332, 519
MwoI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 305
NciI CCSGG 2 cut(s) 38, 143
NdeII GATC 2 cut(s) 223, 687
NlaIV GGNNCC 3 cut(s) 216, 247, 492
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 67
PalAI GGCGCGCC 1 cut(s) 304
PauI GCGCGC 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 139
PfeI GAWTC 4 cut(s) 251, 257, 514, 623
PkrI GCNGC 6 cut(s) 48, 59, 131, 167, 242, 278
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 216, 247, 492
PspPI GGNCC 3 cut(s) 325, 382, 490
PteI GCGCGC 1 cut(s) 304
RseI CAYNNNNRTG 1 cut(s) 657
SacII CCGCGG 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 139
SaqAI TTAA 1 cut(s) 787
SatI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 277
Sau3AI GATC 2 cut(s) 223, 687
Sau96I GGNCC 3 cut(s) 325, 382, 490
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 2 cut(s) 38, 143
SetI ASST 8 cut(s) 98, 160, 330, 349, 354, 619, 756, 781
SfaNI GCATC 4 cut(s) 388, 475, 496, 776
Sfr303I CCGCGG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 187
SgsI GGCGCGCC 1 cut(s) 304
SinI GGWCC 3 cut(s) 325, 382, 490
SmiMI CAYNNNNRTG 1 cut(s) 657
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 5 cut(s) 121, 356, 460, 473, 726
SsiI CCGC 9 cut(s) 127, 130, 165, 184, 186, 197, 243, 277, 488
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 36, 141
TaaI ACNGT 3 cut(s) 219, 233, 749
TaqI TCGA 1 cut(s) 228
TasI AATT 5 cut(s) 121, 356, 460, 473, 726
TauI GCSGC 3 cut(s) 132, 168, 279
TfiI GAWTC 4 cut(s) 251, 257, 514, 623
Tru1I TTAA 1 cut(s) 787
Tru9I TTAA 1 cut(s) 787
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 3 cut(s) 46, 57, 240
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 1 cut(s) 729
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 3 cut(s) 325, 382, 490
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.