RchiOBHm_Chr6g0303031

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011405
Physical Location & Seq
Forward (+)
62907851 .. 62910942
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27221

Sequence Viewer

Length: 912 bp
ATGGCTCAACTGGGAAGACTGCTGGTAAGGCAATGCCCCGCGTTGCAGCACCGCTGCTGCTCCTGCGTCACACTCAATCATCTCTTCTTCTCAACCTCACTTCCACTAAGCACAAACCCTAATTCCCGCCGCTCTTCTTCCCCGGTAATCTACTTAGCTACTCGCGGCGTCCATAAACTAGTCCGCGGACTCCGACCGCGCGACGCTCCTCCGGCGCCGTCCGATCTCGACCGTAACAGCAGCGGTTCCGATTCGGAGTCGTCGATTACATATAAGAGCCGCAACGCCTTGAAACGCGACGCTCGGCGAGCCGTCCGGTGGGCTATGGACCTCGCGTCCTTCTCCACACCTCAGCTCAAATTGATTATCAGAGTGGCTTCTCTGGACCAAGACGTATTGGATGCTGTTATGTTAGTGAAGAGATTTGGGAATGATGTGCGAGAAGGAAAGCGAAGGCAGTTCAATTATATTGCAAAATTGCTACGAGATGCGGACCCTGAGTTGATGGATGCTTTGATTCGCGCTACAAAAGACTGCGACCAAAAGAAGTTGCAAGATTTATGTGGTTCGGAGGTGTTGAGCATTGACGATGAGGAAGAAGAAGAAGAAGAAGCTGAAGAATCTGATAACGAGGAAGAAGAGGGTTCTCACATTGATGTAGCAACCAGATGGGTTGAGGGTATGATCAATAAGGACACTAATATCACCAATGAAGTTTATTCAATTTCAAATGTTGAGTTTGACCGTCAGGAATTGCGGAAACTGATCCGGAAAGTACATTCAGCTTTGGAACGCTCAAGCAATTCTGAGGAAAATAAGGAAAAGATTGATGAAGCGGTAGTGGGTGCCAAAAAATCACTCACCAGGTTCCTACGTGCCCTTGCTAAAAGTAGCCTAATGATTACTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

303

Amino Acids

34.25

Weight (kDa)

6.28

Isoelectric Point (pI)

62.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 91 - 273 2.1e-26 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 214, 845
AccBSI CCGCTC 1 cut(s) 132
AccII CGCG 8 cut(s) 41, 165, 186, 199, 201, 297, 335, 522
AccIII TCCGGA 1 cut(s) 768
AclWI GGATC 1 cut(s) 760
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 2 cut(s) 168, 215
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 1 cut(s) 318
AgsI TTSAA 4 cut(s) 292, 463, 723, 729
AhdI GACNNNNNGTC 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 178
AjnI CCWGG 1 cut(s) 863
AluBI AGCT 4 cut(s) 158, 355, 614, 785
AluI AGCT 4 cut(s) 158, 355, 614, 785
AlwI GGATC 1 cut(s) 760
Aor13HI TCCGGA 1 cut(s) 768
ApeKI GCWGC 4 cut(s) 46, 54, 57, 240
AspLEI GCGC 3 cut(s) 201, 217, 524
AspS9I GGNCC 3 cut(s) 328, 385, 493
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 697, 853
AvaII GGWCC 3 cut(s) 328, 385, 493
BaeGI GKGCMC 1 cut(s) 880
BanI GGYRCC 2 cut(s) 214, 845
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 41, 44, 58, 252
BccI CCATC 2 cut(s) 499, 663
BceAI ACGGC 2 cut(s) 202, 296
BciT130I CCWGG 1 cut(s) 865
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 1 cut(s) 143
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BfoI RGCGCY 1 cut(s) 218
BisI GCNGC 7 cut(s) 47, 55, 58, 130, 166, 241, 280
BlsI GCNGC 7 cut(s) 48, 56, 59, 131, 167, 242, 281
Bme1390I CCNGG 2 cut(s) 143, 865
Bme18I GGWCC 3 cut(s) 328, 385, 493
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 328, 385, 493
BmiI GGNNCC 5 cut(s) 216, 247, 495, 847, 869
BmrFI CCNGG 2 cut(s) 143, 865
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 391, 478, 499
BmuI ACTGGG 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 781
BpuMI CCSGG 1 cut(s) 143
BsaAI YACGTR 1 cut(s) 875
BsaHI GRCGYC 2 cut(s) 168, 215
BsaJI CCNNGG 2 cut(s) 141, 184
BsaWI WCCGGW 2 cut(s) 315, 768
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 38
BseAI TCCGGA 1 cut(s) 768
BseBI CCWGG 1 cut(s) 865
BseDI CCNNGG 2 cut(s) 141, 184
BseGI GGATG 2 cut(s) 406, 514
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 365, 489, 798
BseNI ACTGG 1 cut(s) 15
BseRI GAGGAG 1 cut(s) 198
BseSI GKGCMC 1 cut(s) 880
BseXI GCAGC 4 cut(s) 41, 44, 58, 252
Bsh1236I CGCG 8 cut(s) 41, 165, 186, 199, 201, 297, 335, 522
Bsh1285I CGRYCG 2 cut(s) 197, 232
BshNI GGYRCC 2 cut(s) 214, 845
BsiEI CGRYCG 2 cut(s) 197, 232
BsiSI CCGG 4 cut(s) 143, 212, 316, 769
BslI CCNNNNNNNGG 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 880
Bsp13I TCCGGA 1 cut(s) 768
Bsp143I GATC 3 cut(s) 223, 684, 765
BspCNI CTCAG 3 cut(s) 364, 490, 799
BspEI TCCGGA 1 cut(s) 768
BspFNI CGCG 8 cut(s) 41, 165, 186, 199, 201, 297, 335, 522
BspLI GGNNCC 5 cut(s) 216, 247, 495, 847, 869
BspPI GGATC 1 cut(s) 760
BspQI GCTCTTC 1 cut(s) 139
BspT107I GGYRCC 2 cut(s) 214, 845
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 141, 184
BssMI GATC 3 cut(s) 223, 684, 765
BssNI GRCGYC 2 cut(s) 168, 215
Bst2UI CCWGG 1 cut(s) 865
Bst4CI ACNGT 2 cut(s) 233, 746
Bst6I CTCTTC 4 cut(s) 89, 139, 413, 633
BstACI GRCGYC 2 cut(s) 168, 215
BstBAI YACGTR 1 cut(s) 875
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 5 cut(s) 107, 154, 351, 498, 807
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 406, 514
BstFNI CGCG 8 cut(s) 41, 165, 186, 199, 201, 297, 335, 522
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 3 cut(s) 201, 217, 524
BstKTI GATC 3 cut(s) 226, 687, 768
BstMBI GATC 3 cut(s) 223, 684, 765
BstMCI CGRYCG 2 cut(s) 197, 232
BstMWI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
BstNI CCWGG 1 cut(s) 865
BstSCI CCNGG 2 cut(s) 141, 863
BstSLI GKGCMC 1 cut(s) 880
BstUI CGCG 8 cut(s) 41, 165, 186, 199, 201, 297, 335, 522
BstV1I GCAGC 4 cut(s) 41, 44, 58, 252
BstV2I GAAGAC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 406, 514
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 3 cut(s) 201, 217, 524
Cfr13I GGNCC 3 cut(s) 328, 385, 493
Cfr42I CCGCGG 1 cut(s) 187
CseI GACGC 5 cut(s) 55, 157, 212, 308, 324
CsiI ACCWGGT 1 cut(s) 863
Csp6I GTAC 1 cut(s) 776
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 5 cut(s) 107, 154, 351, 498, 807
DinI GGCGCC 1 cut(s) 216
DpnI GATC 3 cut(s) 225, 686, 767
DpnII GATC 3 cut(s) 223, 684, 765
DriI GACNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 4 cut(s) 89, 139, 413, 633
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 4 cut(s) 89, 139, 413, 633
Eco47I GGWCC 3 cut(s) 328, 385, 493
Eco57I CTGAAG 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 863
EgeI GGCGCC 1 cut(s) 216
EheI GGCGCC 1 cut(s) 216
FaiI YATR 9 cut(s) 174, 271, 273, 326, 410, 468, 562, 683, 910
FauI CCCGC 2 cut(s) 46, 134
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 7 cut(s) 47, 55, 58, 130, 166, 241, 280
FokI GGATG 2 cut(s) 413, 521
Fsp4HI GCNGC 7 cut(s) 47, 55, 58, 130, 166, 241, 280
FspBI CTAG 1 cut(s) 179
GlaI GCGC 3 cut(s) 200, 216, 523
GluI GCNGC 7 cut(s) 47, 55, 58, 130, 166, 241, 280
HaeII RGCGCY 1 cut(s) 218
HapII CCGG 4 cut(s) 143, 212, 316, 769
HgaI GACGC 5 cut(s) 55, 157, 212, 308, 324
HhaI GCGC 3 cut(s) 201, 217, 524
Hin1I GRCGYC 2 cut(s) 168, 215
Hin6I GCGC 3 cut(s) 199, 215, 522
HinP1I GCGC 3 cut(s) 199, 215, 522
HinfI GANTC 5 cut(s) 189, 251, 257, 517, 620
HpaII CCGG 4 cut(s) 143, 212, 316, 769
HphI GGTGA 2 cut(s) 697, 853
Hpy188I TCNGA 8 cut(s) 194, 223, 250, 256, 371, 571, 625, 808
Hpy188III TCNNGA 4 cut(s) 227, 383, 749, 769
Hpy99I CGWCG 3 cut(s) 206, 265, 302
HpyAV CCTTC 3 cut(s) 349, 437, 447
HpyCH4III ACNGT 2 cut(s) 233, 746
HpyCH4IV ACGT 2 cut(s) 393, 874
HpyCH4V TGCA 3 cut(s) 46, 473, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
HpyF3I CTNAG 5 cut(s) 107, 154, 351, 498, 807
HpySE526I ACGT 2 cut(s) 393, 874
Hsp92I GRCGYC 2 cut(s) 168, 215
HspAI GCGC 3 cut(s) 199, 215, 522
KasI GGCGCC 1 cut(s) 214
Kpn2I TCCGGA 1 cut(s) 768
Ksp22I TGATCA 1 cut(s) 684
KspI CCGCGG 1 cut(s) 187
Kzo9I GATC 3 cut(s) 223, 684, 765
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 2 cut(s) 65, 211
Lsp1109I GCAGC 4 cut(s) 41, 44, 58, 252
LweI GCATC 3 cut(s) 391, 478, 499
MabI ACCWGGT 1 cut(s) 863
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 393, 874
MaeIII GTNAC 2 cut(s) 67, 233
MalI GATC 3 cut(s) 225, 686, 767
MbiI CCGCTC 1 cut(s) 132
MboI GATC 3 cut(s) 223, 684, 765
MhlI GDGCHC 1 cut(s) 880
MluCI AATT 7 cut(s) 121, 359, 463, 476, 723, 752, 802
Mly113I GGCGCC 1 cut(s) 215
MlyI GAGTC 2 cut(s) 183, 266
MmeI TCCRAC 1 cut(s) 217
MroI TCCGGA 1 cut(s) 768
MslI CAYNNNNRTG 1 cut(s) 654
MspA1I CMGCKG 3 cut(s) 54, 186, 243
MspI CCGG 4 cut(s) 143, 212, 316, 769
MspR9I CCNGG 2 cut(s) 143, 865
MvaI CCWGG 1 cut(s) 865
MvnI CGCG 8 cut(s) 41, 165, 186, 199, 201, 297, 335, 522
MwoI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
NarI GGCGCC 1 cut(s) 215
NciI CCSGG 1 cut(s) 143
NdeII GATC 3 cut(s) 223, 684, 765
NlaIV GGNNCC 5 cut(s) 216, 247, 495, 847, 869
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PfeI GAWTC 3 cut(s) 251, 517, 620
PkrI GCNGC 7 cut(s) 48, 56, 59, 131, 167, 242, 281
PleI GAGTC 2 cut(s) 183, 265
PluTI GGCGCC 1 cut(s) 218
PpsI GAGTC 2 cut(s) 183, 265
Ppu21I YACGTR 1 cut(s) 875
Psp6I CCWGG 1 cut(s) 863
PspGI CCWGG 1 cut(s) 863
PspN4I GGNNCC 5 cut(s) 216, 247, 495, 847, 869
PspPI GGNCC 3 cut(s) 328, 385, 493
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
RseI CAYNNNNRTG 1 cut(s) 654
SacII CCGCGG 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 139
SatI GCNGC 7 cut(s) 47, 55, 58, 130, 166, 241, 280
Sau3AI GATC 3 cut(s) 223, 684, 765
Sau96I GGNCC 3 cut(s) 328, 385, 493
SchI GAGTC 2 cut(s) 183, 266
ScrFI CCNGG 2 cut(s) 143, 865
SduI GDGCHC 1 cut(s) 880
SexAI ACCWGGT 1 cut(s) 863
SfaNI GCATC 3 cut(s) 391, 478, 499
SfoI GGCGCC 1 cut(s) 216
Sfr303I CCGCGG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 187
SinI GGWCC 3 cut(s) 328, 385, 493
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 1 cut(s) 796
SmoI CTYRAG 1 cut(s) 796
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 7 cut(s) 121, 359, 463, 476, 723, 752, 802
SspDI GGCGCC 1 cut(s) 214
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 141, 863
TaaI ACNGT 2 cut(s) 233, 746
TaiI ACGT 2 cut(s) 396, 877
TaqI TCGA 2 cut(s) 228, 263
TasI AATT 7 cut(s) 121, 359, 463, 476, 723, 752, 802
TatI WGTACW 1 cut(s) 775
TauI GCSGC 3 cut(s) 132, 168, 282
TfiI GAWTC 3 cut(s) 251, 517, 620
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 4 cut(s) 46, 54, 57, 240
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 2 cut(s) 726, 846
VpaK11BI GGWCC 3 cut(s) 328, 385, 493
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.