Rh6CG444800

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0011405
Physical Location & Seq
Forward (+)
62311708 .. 62315627
3920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG444800.1

Sequence Viewer

Length: 783 bp
ATGGCTCAACTGGGAAGACTGCTGGTAAGGCAATGCCCGGCGTTGCAGCACCACTGCTGCTCCTGCGTCACACTCAATCATCTCTTCTTCTCAACCTCACTTCCACTAAGCACAAACCCTAATTCCCGCCACTCTTCTTCCCCGGTAATCTACTTAGCTACTCGCGGCGTCCATAAACTAGTCCGCGGACTCCGACCGCGCGACGCTCCTCCGGCACCGTCCGATCTCGAAGGTAACAGCAGCGGTTCCGATTCGGAGTCGTCGATTACATATAAGAGCCGCAACGCCTTGAAACGCGACGCTCGGCGCGCCGTCCGGTGGGCTATGGACCTCGCGTCCTTCTCTACACCTCAGCTCAAATTGATTATCAGAGTGGCTTCTCTGGACCAAGACGTATTGGATGCTGTTATGTTAGTGAAGAGATTTGGGAATGATGTGCGAGAAGGAAAGCGAAGGCAGTTCAATTATATTGCAAAATTGCTACGAGATGCAGACCCTGAGTTGATGGATGCTTTGATTCGCGCTACAAAAGACTGCGACCAAAAGAAGTTGCAAGATTTATGTGGTTCGGAGGCGTTGAGCATTGACGATGAGGAAGAAGAAGAAGAAGCTGAAGAATCTGATAACGAGGAAGAAGAGGGTTCTCACATTGATGTAGCAACCAGATGGGTTGAGGGTATGATCAATAAGGACACTAATATCACCAATGAAGTTTATTCAATTTCAAATGTTGAGTTTGACCGTCAGGTGAGTGGAATGGCATCTCCACAACCTTGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

260

Amino Acids

29.06

Weight (kDa)

5.25

Isoelectric Point (pI)

63.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 91 - 241 4.6e-21 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AccII CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
AciI CCGC 7 cut(s) 127, 165, 184, 186, 197, 243, 280
AcuI CTGAAG 1 cut(s) 633
AcyI GRCGYC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 318
AgsI TTSAA 4 cut(s) 292, 463, 720, 726
AhdI GACNNNNNGTC 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 178
AluBI AGCT 3 cut(s) 158, 355, 611
AluI AGCT 3 cut(s) 158, 355, 611
AlwNI CAGNNNCTG 1 cut(s) 497
ApeKI GCWGC 3 cut(s) 46, 57, 240
AscI GGCGCGCC 1 cut(s) 307
AspLEI GCGC 4 cut(s) 201, 309, 311, 524
AspS9I GGNCC 2 cut(s) 328, 385
AsuC2I CCSGG 2 cut(s) 38, 143
AsuHPI GGTGA 2 cut(s) 694, 760
AvaII GGWCC 2 cut(s) 328, 385
BanI GGYRCC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 3 cut(s) 44, 58, 252
BccI CCATC 2 cut(s) 499, 660
BceAI ACGGC 1 cut(s) 296
BclI TGATCA 1 cut(s) 681
BcnI CCSGG 2 cut(s) 38, 143
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BisI GCNGC 5 cut(s) 47, 58, 166, 241, 280
BlsI GCNGC 5 cut(s) 48, 59, 167, 242, 281
Bme1390I CCNGG 2 cut(s) 38, 143
Bme18I GGWCC 2 cut(s) 328, 385
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 328, 385
BmiI GGNNCC 2 cut(s) 216, 247
BmrFI CCNGG 2 cut(s) 38, 143
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 4 cut(s) 391, 478, 499, 770
BmuI ACTGGG 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 351
BpuMI CCSGG 2 cut(s) 38, 143
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 141, 184
BsaWI WCCGGW 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 38
BseDI CCNNGG 2 cut(s) 141, 184
BseGI GGATG 2 cut(s) 406, 514
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 2 cut(s) 365, 489
BseNI ACTGG 1 cut(s) 15
BsePI GCGCGC 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 3 cut(s) 44, 58, 252
Bsh1236I CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 197
BsiSI CCGG 4 cut(s) 38, 143, 212, 316
BslI CCNNNNNNNGG 1 cut(s) 318
Bsp143I GATC 2 cut(s) 223, 681
BspACI CCGC 7 cut(s) 127, 165, 184, 186, 197, 243, 280
BspCNI CTCAG 2 cut(s) 364, 490
BspFNI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BspLI GGNNCC 2 cut(s) 216, 247
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 141, 184
BssHII GCGCGC 1 cut(s) 307
BssMI GATC 2 cut(s) 223, 681
BssNI GRCGYC 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 219, 743
Bst6I CTCTTC 4 cut(s) 89, 139, 413, 630
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 4 cut(s) 107, 154, 351, 498
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 406, 514
BstFNI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BstHHI GCGC 4 cut(s) 201, 309, 311, 524
BstKTI GATC 2 cut(s) 226, 684
BstMBI GATC 2 cut(s) 223, 681
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
BstSCI CCNGG 2 cut(s) 36, 141
BstUI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BstV1I GCAGC 3 cut(s) 44, 58, 252
BstV2I GAAGAC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 406, 514
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 497
CfoI GCGC 4 cut(s) 201, 309, 311, 524
Cfr13I GGNCC 2 cut(s) 328, 385
Cfr42I CCGCGG 1 cut(s) 187
CseI GACGC 5 cut(s) 55, 157, 212, 308, 324
CviJI RGCY 7 cut(s) 5, 158, 279, 323, 355, 377, 611
CviKI_1 RGCY 7 cut(s) 5, 158, 279, 323, 355, 377, 611
DdeI CTNAG 4 cut(s) 107, 154, 351, 498
DpnI GATC 2 cut(s) 225, 683
DpnII GATC 2 cut(s) 223, 681
DriI GACNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 4 cut(s) 89, 139, 413, 630
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 4 cut(s) 89, 139, 413, 630
Eco47I GGWCC 2 cut(s) 328, 385
Eco57I CTGAAG 1 cut(s) 633
FaiI YATR 8 cut(s) 174, 271, 273, 326, 410, 468, 562, 680
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 681
Fnu4HI GCNGC 5 cut(s) 47, 58, 166, 241, 280
FokI GGATG 2 cut(s) 413, 521
Fsp4HI GCNGC 5 cut(s) 47, 58, 166, 241, 280
FspBI CTAG 1 cut(s) 179
GlaI GCGC 4 cut(s) 200, 308, 310, 523
GluI GCNGC 5 cut(s) 47, 58, 166, 241, 280
HapII CCGG 4 cut(s) 38, 143, 212, 316
HgaI GACGC 5 cut(s) 55, 157, 212, 308, 324
HhaI GCGC 4 cut(s) 201, 309, 311, 524
Hin1I GRCGYC 1 cut(s) 168
Hin6I GCGC 4 cut(s) 199, 307, 309, 522
HinP1I GCGC 4 cut(s) 199, 307, 309, 522
HinfI GANTC 5 cut(s) 189, 251, 257, 517, 617
HpaII CCGG 4 cut(s) 38, 143, 212, 316
HphI GGTGA 2 cut(s) 694, 760
Hpy188I TCNGA 7 cut(s) 194, 223, 250, 256, 371, 571, 622
Hpy188III TCNNGA 2 cut(s) 227, 383
Hpy99I CGWCG 3 cut(s) 206, 265, 302
HpyAV CCTTC 4 cut(s) 224, 349, 437, 447
HpyCH4III ACNGT 2 cut(s) 219, 743
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 4 cut(s) 46, 473, 491, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
HpyF3I CTNAG 4 cut(s) 107, 154, 351, 498
HpySE526I ACGT 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 168
HspAI GCGC 4 cut(s) 199, 307, 309, 522
Ksp22I TGATCA 1 cut(s) 681
KspI CCGCGG 1 cut(s) 187
Kzo9I GATC 2 cut(s) 223, 681
LmnI GCTCC 2 cut(s) 65, 211
Lsp1109I GCAGC 3 cut(s) 44, 58, 252
LweI GCATC 4 cut(s) 391, 478, 499, 770
MaeI CTAG 1 cut(s) 179
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 67, 233
MalI GATC 2 cut(s) 225, 683
MboI GATC 2 cut(s) 223, 681
MluCI AATT 5 cut(s) 121, 359, 463, 476, 720
MlyI GAGTC 2 cut(s) 183, 266
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 9 cut(s) 106, 219, 341, 360, 565, 586, 622, 631, 667
MseI TTAA 1 cut(s) 781
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 2 cut(s) 186, 243
MspI CCGG 4 cut(s) 38, 143, 212, 316
MspR9I CCNGG 2 cut(s) 38, 143
MvnI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
MwoI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
NciI CCSGG 2 cut(s) 38, 143
NdeII GATC 2 cut(s) 223, 681
NlaIV GGNNCC 2 cut(s) 216, 247
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 67
PalAI GGCGCGCC 1 cut(s) 307
PauI GCGCGC 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PfeI GAWTC 3 cut(s) 251, 517, 617
PkrI GCNGC 5 cut(s) 48, 59, 167, 242, 281
PleI GAGTC 2 cut(s) 183, 265
PpsI GAGTC 2 cut(s) 183, 265
PspN4I GGNNCC 2 cut(s) 216, 247
PspPI GGNCC 2 cut(s) 328, 385
PstNI CAGNNNCTG 1 cut(s) 497
PteI GCGCGC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 651
SacII CCGCGG 1 cut(s) 187
SaqAI TTAA 1 cut(s) 781
SatI GCNGC 5 cut(s) 47, 58, 166, 241, 280
Sau3AI GATC 2 cut(s) 223, 681
Sau96I GGNCC 2 cut(s) 328, 385
SchI GAGTC 2 cut(s) 183, 266
ScrFI CCNGG 2 cut(s) 38, 143
SfaNI GCATC 4 cut(s) 391, 478, 499, 770
Sfr303I CCGCGG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 187
SgsI GGCGCGCC 1 cut(s) 307
SinI GGWCC 2 cut(s) 328, 385
SmiMI CAYNNNNRTG 1 cut(s) 651
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 5 cut(s) 121, 359, 463, 476, 720
SsiI CCGC 7 cut(s) 127, 165, 184, 186, 197, 243, 280
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 36, 141
TaaI ACNGT 2 cut(s) 219, 743
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 228, 263
TasI AATT 5 cut(s) 121, 359, 463, 476, 720
TauI GCSGC 2 cut(s) 168, 282
TfiI GAWTC 3 cut(s) 251, 517, 617
Tru1I TTAA 1 cut(s) 781
Tru9I TTAA 1 cut(s) 781
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 3 cut(s) 46, 57, 240
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 1 cut(s) 723
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 2 cut(s) 328, 385
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.