pycom08g01050

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011405
Physical Location & Seq
Reverse (-)
825334 .. 826997
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01050.4

Sequence Viewer

Length: 936 bp
ATGGCTGCTCACGCGGGACGGCTTCTGAGGCAATGGCCGATGTGGCAGCAGCGTTGCTGCTCAAACGCCGCCTTCCACTACCTTATCTTCTCCCCCTTACTTCCGGTCACCACGAGAACCAACTCCCGTCGTCCGTCTTTGACCACCGTCCACCTGGCCACTCGCGGCGTCCATAAACTATTACGCGGGCTCAGACCGTCCGATTACCAAGACGCCAACACCGCCAGTTCGGGTTCAGGTTCGGATTCGGATGCAGTCACGTACAAGAGCCGCAACGAGTTGAAGCGCCACGCCCGACGCGCCGTCCGTTGGGCTATGGACCTCGCCTCCTTCTCCACCCCTCAAATCAAACGCATTGTCAGAGTGGCTTCTCTGGACCAAGACGTGTTGGATGCTGTGATCCTAGTGAAGGTGCTTGCTGAGGTGTTTGTGTTAATGCCGCAGAAACTAGGACCTGATGTTCGAGAAGGGAAGCGAAGGCAGTTCAATTATATTGGGAAACTGCTGCGGGATGTGGAGGCCGACTTGATGGATGCTTTGATTCAGGCTACGAAAGATAGCGATGAAAGTAAGCTGCAAGCTTTATCAGGTCCGGAGACATTGGGGGTTGATGATGTTGCCGACGAAGAAGAAGCGGAAGAAACAGATTGTGAGGAGGAAGACGAGGGTTCTCACATTGATGAAGCGACCAGATGGTTTGATGGTCTGATCAGTAAGGACATCAAGATCACCAATGAAGTTTATTCAATTTCGAATGTTGATTTTGACCGTCAGGAACTAAGGAAACTTGTCCGAAGTGTGCATTCTAGATTGGAACAGAAGGTTGATTTGGAGGAAAATACGGGAAGGATTGGTGCAGCGAGAGTGAGTGCTGAAAAGGCACTCACCAGGTTCCTTCGTTCCCTTGCTAAACGTAGCCTAGAGATTGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

312

Amino Acids

35.09

Weight (kDa)

6.21

Isoelectric Point (pI)

52.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 14, 165, 186, 300
AccIII TCCGGA 1 cut(s) 592
AciI CCGC 9 cut(s) 14, 69, 165, 186, 222, 271, 440, 508, 635
AclWI GGATC 1 cut(s) 394
AcoI YGGCCR 2 cut(s) 35, 156
AcyI GRCGYC 2 cut(s) 168, 213
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 2 cut(s) 309, 409
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 3 cut(s) 283, 487, 747
AhdI GACNNNNNGTC 1 cut(s) 302
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 2 cut(s) 153, 887
AjuI GAANNNNNNNTTGG 2 cut(s) 812, 844
AloI GAACNNNNNNTCC 2 cut(s) 444, 476
AluBI AGCT 2 cut(s) 574, 581
AluI AGCT 2 cut(s) 574, 581
Alw26I GTCTC 1 cut(s) 590
AlwI GGATC 1 cut(s) 394
Aor13HI TCCGGA 1 cut(s) 592
AoxI GGCC 3 cut(s) 35, 156, 519
ApeKI GCWGC 7 cut(s) 5, 46, 49, 57, 505, 574, 857
ArsI GACNNNNNNTTYG 4 cut(s) 342, 374, 444, 476
AspLEI GCGC 2 cut(s) 288, 302
AspS9I GGNCC 4 cut(s) 319, 376, 452, 590
AsuHPI GGTGA 3 cut(s) 100, 721, 877
AsuII TTCGAA 1 cut(s) 752
AvaII GGWCC 4 cut(s) 319, 376, 452, 590
BalI TGGCCA 1 cut(s) 158
BanII GRGCYC 1 cut(s) 192
BauI CACGAG 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 666
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 6 cut(s) 44, 58, 61, 492, 561, 869
BccI CCATC 3 cut(s) 523, 687, 695
BceAI ACGGC 2 cut(s) 35, 287
BciT130I CCWGG 2 cut(s) 155, 889
BclI TGATCA 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 590
BfaI CTAG 4 cut(s) 404, 449, 807, 920
BfoI RGCGCY 1 cut(s) 289
BglI GCCNNNNNGGC 1 cut(s) 43
Bme1390I CCNGG 2 cut(s) 155, 889
Bme18I GGWCC 4 cut(s) 319, 376, 452, 590
BmeRI GACNNNNNGTC 1 cut(s) 302
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 4 cut(s) 319, 376, 452, 590
BmiI GGNNCC 1 cut(s) 893
BmrFI CCNGG 2 cut(s) 155, 889
BmsI GCATC 3 cut(s) 241, 382, 523
BoxI GACNNNNGTC 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 666
Bpu10I CCTNAGC 1 cut(s) 420
Bpu14I TTCGAA 1 cut(s) 752
BsaAI YACGTR 1 cut(s) 261
BsaHI GRCGYC 2 cut(s) 168, 213
BsaWI WCCGGW 2 cut(s) 103, 592
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 409
Bse1I ACTGG 1 cut(s) 225
Bse3DI GCAATG 1 cut(s) 38
BseAI TCCGGA 1 cut(s) 592
BseBI CCWGG 2 cut(s) 155, 889
BseGI GGATG 4 cut(s) 256, 397, 517, 538
BseLI CCNNNNNNNGG 2 cut(s) 309, 409
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 17, 205, 411
BseNI ACTGG 1 cut(s) 225
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 6 cut(s) 44, 58, 61, 492, 561, 869
BsgI GTGCAG 1 cut(s) 876
Bsh1236I CGCG 4 cut(s) 14, 165, 186, 300
BshFI GGCC 3 cut(s) 37, 158, 521
BsiSI CCGG 2 cut(s) 104, 593
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 309, 409
BsmAI GTCTC 1 cut(s) 590
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 1 cut(s) 802
BsnI GGCC 3 cut(s) 37, 158, 521
Bsp119I TTCGAA 1 cut(s) 752
Bsp1286I GDGCHC 1 cut(s) 192
Bsp13I TCCGGA 1 cut(s) 592
Bsp143I GATC 3 cut(s) 399, 708, 726
BspACI CCGC 9 cut(s) 14, 69, 165, 186, 222, 271, 440, 508, 635
BspANI GGCC 3 cut(s) 37, 158, 521
BspCNI CTCAG 3 cut(s) 18, 204, 412
BspEI TCCGGA 1 cut(s) 592
BspFNI CGCG 4 cut(s) 14, 165, 186, 300
BspLI GGNNCC 1 cut(s) 893
BspPI GGATC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 1 cut(s) 225
BssMI GATC 3 cut(s) 399, 708, 726
BssNI GRCGYC 2 cut(s) 168, 213
BssSI CACGAG 1 cut(s) 112
Bst2BI CACGAG 1 cut(s) 112
Bst2UI CCWGG 2 cut(s) 155, 889
Bst4CI ACNGT 3 cut(s) 148, 198, 770
BstACI GRCGYC 2 cut(s) 168, 213
BstBAI YACGTR 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 752
BstC8I GCNNGC 3 cut(s) 188, 417, 579
BstDEI CTNAG 4 cut(s) 26, 191, 420, 779
BstEII GGTNACC 1 cut(s) 106
BstENI CCTNNNNNAGG 1 cut(s) 407
BstF5I GGATG 4 cut(s) 256, 397, 517, 538
BstFNI CGCG 4 cut(s) 14, 165, 186, 300
BstH2I RGCGCY 1 cut(s) 289
BstHHI GCGC 2 cut(s) 288, 302
BstKTI GATC 3 cut(s) 402, 711, 729
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 3 cut(s) 399, 708, 726
BstMWI GCNNNNNNNGC 6 cut(s) 11, 28, 43, 221, 299, 878
BstNI CCWGG 2 cut(s) 155, 889
BstPAI GACNNNNGTC 1 cut(s) 146
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 2 cut(s) 153, 887
BstUI CGCG 4 cut(s) 14, 165, 186, 300
BstV1I GCAGC 6 cut(s) 44, 58, 61, 492, 561, 869
BstV2I GAAGAC 1 cut(s) 666
BsuRI GGCC 3 cut(s) 37, 158, 521
BtgZI GCGATG 1 cut(s) 576
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 4 cut(s) 256, 397, 517, 538
Cac8I GCNNGC 3 cut(s) 188, 417, 579
CfoI GCGC 2 cut(s) 288, 302
Cfr13I GGNCC 4 cut(s) 319, 376, 452, 590
CseI GACGC 3 cut(s) 157, 221, 306
CsiI ACCWGGT 1 cut(s) 887
Csp6I GTAC 1 cut(s) 262
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 4 cut(s) 26, 191, 420, 779
DpnI GATC 3 cut(s) 401, 710, 728
DpnII GATC 3 cut(s) 399, 708, 726
DriI GACNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 2 cut(s) 35, 156
Eam1105I GACNNNNNGTC 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 4 cut(s) 319, 376, 452, 590
Eco91I GGTNACC 1 cut(s) 106
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 452
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 153, 887
EcoT38I GRGCYC 1 cut(s) 192
FaiI YATR 5 cut(s) 174, 317, 492, 932, 934
FaqI GGGAC 1 cut(s) 30
FauI CCCGC 3 cut(s) 7, 179, 501
FbaI TGATCA 1 cut(s) 708
FokI GGATG 4 cut(s) 263, 404, 524, 545
FriOI GRGCYC 1 cut(s) 192
FspBI CTAG 4 cut(s) 404, 449, 807, 920
GlaI GCGC 2 cut(s) 287, 301
HaeII RGCGCY 1 cut(s) 289
HaeIII GGCC 3 cut(s) 37, 158, 521
HapII CCGG 2 cut(s) 104, 593
HgaI GACGC 3 cut(s) 157, 221, 306
HhaI GCGC 2 cut(s) 288, 302
Hin1I GRCGYC 2 cut(s) 168, 213
Hin6I GCGC 2 cut(s) 286, 300
HinP1I GCGC 2 cut(s) 286, 300
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 2 cut(s) 245, 541
HpaII CCGG 2 cut(s) 104, 593
HphI GGTGA 3 cut(s) 100, 721, 877
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 8 cut(s) 27, 194, 202, 244, 250, 362, 708, 794
Hpy188III TCNNGA 6 cut(s) 374, 464, 593, 724, 773, 807
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 3 cut(s) 132, 300, 626
HpyAV CCTTC 8 cut(s) 82, 340, 403, 461, 471, 814, 840, 905
HpyCH4III ACNGT 3 cut(s) 148, 198, 770
HpyCH4IV ACGT 3 cut(s) 260, 384, 913
HpyCH4V TGCA 4 cut(s) 254, 577, 802, 857
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 28, 43, 221, 299, 878
HpyF3I CTNAG 4 cut(s) 26, 191, 420, 779
HpySE526I ACGT 3 cut(s) 260, 384, 913
Hsp92I GRCGYC 2 cut(s) 168, 213
HspAI GCGC 2 cut(s) 286, 300
Kpn2I TCCGGA 1 cut(s) 592
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 3 cut(s) 399, 708, 726
Lsp1109I GCAGC 6 cut(s) 44, 58, 61, 492, 561, 869
LweI GCATC 3 cut(s) 241, 382, 523
MabI ACCWGGT 1 cut(s) 887
MaeI CTAG 4 cut(s) 404, 449, 807, 920
MaeII ACGT 3 cut(s) 260, 384, 913
MaeIII GTNAC 2 cut(s) 106, 256
MalI GATC 3 cut(s) 401, 710, 728
MboI GATC 3 cut(s) 399, 708, 726
MboII GAAGA 5 cut(s) 79, 638, 641, 650, 671
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 2 cut(s) 487, 747
MluNI TGGCCA 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 158
MroI TCCGGA 1 cut(s) 592
MscI TGGCCA 1 cut(s) 158
MseI TTAA 1 cut(s) 434
MslI CAYNNNNRTG 1 cut(s) 678
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 2 cut(s) 104, 593
MspR9I CCNGG 2 cut(s) 155, 889
Mva1269I GAATGC 1 cut(s) 802
MvaI CCWGG 2 cut(s) 155, 889
MvnI CGCG 4 cut(s) 14, 165, 186, 300
MwoI GCNNNNNNNGC 6 cut(s) 11, 28, 43, 221, 299, 878
NdeII GATC 3 cut(s) 399, 708, 726
NlaIV GGNNCC 1 cut(s) 893
NmuCI GTSAC 2 cut(s) 106, 256
NspV TTCGAA 1 cut(s) 752
PcsI WCGNNNNNNNCGW 1 cut(s) 304
PctI GAATGC 1 cut(s) 802
PfeI GAWTC 2 cut(s) 245, 541
Ppu21I YACGTR 1 cut(s) 261
PpuMI RGGWCCY 1 cut(s) 452
PshAI GACNNNNGTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 452
Psp6I CCWGG 2 cut(s) 153, 887
PspEI GGTNACC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 153, 887
PspN4I GGNNCC 1 cut(s) 893
PspPI GGNCC 4 cut(s) 319, 376, 452, 590
PspPPI RGGWCCY 1 cut(s) 452
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 678
SaqAI TTAA 1 cut(s) 434
Sau3AI GATC 3 cut(s) 399, 708, 726
Sau96I GGNCC 4 cut(s) 319, 376, 452, 590
ScrFI CCNGG 2 cut(s) 155, 889
SduI GDGCHC 1 cut(s) 192
SexAI ACCWGGT 1 cut(s) 887
SfaNI GCATC 3 cut(s) 241, 382, 523
SfuI TTCGAA 1 cut(s) 752
SinI GGWCC 4 cut(s) 319, 376, 452, 590
SmiMI CAYNNNNRTG 1 cut(s) 678
Sse9I AATT 2 cut(s) 487, 747
SsiI CCGC 9 cut(s) 14, 69, 165, 186, 222, 271, 440, 508, 635
SspMI CTAG 4 cut(s) 404, 449, 807, 920
StyD4I CCNGG 2 cut(s) 153, 887
TaaI ACNGT 3 cut(s) 148, 198, 770
TaiI ACGT 3 cut(s) 263, 387, 916
TaqI TCGA 2 cut(s) 463, 752
TasI AATT 2 cut(s) 487, 747
TauI GCSGC 4 cut(s) 71, 168, 273, 442
TfiI GAWTC 2 cut(s) 245, 541
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TseFI GTSAC 2 cut(s) 106, 256
TseI GCWGC 7 cut(s) 5, 46, 49, 57, 505, 574, 857
Tsp45I GTSAC 2 cut(s) 106, 256
TspDTI ATGAA 3 cut(s) 579, 696, 750
TspGWI ACGGA 2 cut(s) 123, 296
VpaK11BI GGWCC 4 cut(s) 319, 376, 452, 590
XagI CCTNNNNNAGG 1 cut(s) 407
XbaI TCTAGA 1 cut(s) 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XspI CTAG 4 cut(s) 404, 449, 807, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.