MD08G1012000.v1.1

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011405
Physical Location & Seq
Reverse (-)
918159 .. 919966
1808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1012000.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGCTGCTCAAGCGGGACGGCTACTGAGGCAATGGCCGATGTGGCAGCAGCATTGCTGCTCAAACGCCGCCTTCCACCACCTTATCTTCTCCCCTTTACTTCCGGTCACCACCAGAACCAACTCCCGTCGTCCGTCTTTGACCACCGTCCACCTGGCCACTCGCGGCGTCCATAAACTATTACGCGGTCTCAGACCGTCCGATTACCAAGACGCCAACACAGCCAGTTCGGGTTCGGGTTCGGATTCGGATTCAGTCACGTACAAGAGCCGTAACGAGTTGAAGCGCCACGCCCGCCGCGCCGTCCGTTGGGCTATGGACCTCGCGTCCTTCTCCACCCCTCAAATCAAGCGCATTGTCAGAGTGGCTTCTCTGGACCAAGACGTGTTGGATGCTGTGATCCTAGTGAAGAAATTAGGACCGGATGTTCGAGAAGGGAAGCGAAGGCAGTTCAATTATATTGGGAAACTGCTGCGGGATGTGGAGGCCGACTTGATGGATGCTTTGATTCAGGCTACGAAAGATAGCGACGAAAGTAAGCTGCAAGCTTTATCAGGTCCGGAGACATTGGAGGTTGATGATGTTGCCGACAAAGAAGAGGCGGAAGAAACTGATTGTGAGGAGGAAGAAGAGGGTTCTCACATTGATGAAGCAACCAGATGGTTTGATGGTCTGATCAGTAAGGACATTAAGATCACCAATGAAGTTTATTCAATTTCGAATGTTGATTTTGACCGTCAGGAACTAAGGAAACTTGTCCGAAGTGTGCATTCTAGATTGGAACAGAAGGTTGATTTGGAGGAAAATACGGGAAAGATTGATGCAGTGAGAGTGAGTGCTGAAAAGGCACTCACCAGGTTCCTTCTTTCCCTTGCTAAACGTAGCCTAGAGACTGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

301

Amino Acids

33.91

Weight (kDa)

6.11

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 88 - 263 2.9e-27 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 165, 186, 300, 326
AccIII TCCGGA 1 cut(s) 559
AciI CCGC 8 cut(s) 14, 69, 165, 186, 295, 298, 475, 602
AclWI GGATC 1 cut(s) 394
AcoI YGGCCR 2 cut(s) 35, 156
AcyI GRCGYC 2 cut(s) 168, 213
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 3 cut(s) 283, 454, 714
AhdI GACNNNNNGTC 1 cut(s) 325
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 2 cut(s) 153, 854
AjuI GAANNNNNNNTTGG 2 cut(s) 779, 811
AloI GAACNNNNNNTCC 2 cut(s) 411, 443
AluBI AGCT 2 cut(s) 541, 548
AluI AGCT 2 cut(s) 541, 548
Alw26I GTCTC 3 cut(s) 194, 557, 884
AlwI GGATC 1 cut(s) 394
Aor13HI TCCGGA 1 cut(s) 559
AoxI GGCC 3 cut(s) 35, 156, 486
ApeKI GCWGC 6 cut(s) 5, 46, 49, 57, 472, 541
ArsI GACNNNNNNTTYG 2 cut(s) 411, 443
AspLEI GCGC 3 cut(s) 288, 302, 354
AspS9I GGNCC 4 cut(s) 319, 376, 419, 557
AsuHPI GGTGA 3 cut(s) 100, 688, 844
AsuII TTCGAA 1 cut(s) 719
AvaII GGWCC 4 cut(s) 319, 376, 419, 557
BalI TGGCCA 1 cut(s) 158
BbvI GCAGC 5 cut(s) 44, 58, 61, 459, 528
BccI CCATC 3 cut(s) 490, 654, 662
BceAI ACGGC 3 cut(s) 35, 255, 287
BciT130I CCWGG 2 cut(s) 155, 856
BclI TGATCA 1 cut(s) 675
BcoDI GTCTC 3 cut(s) 194, 557, 884
BfaI CTAG 3 cut(s) 404, 774, 887
BfoI RGCGCY 1 cut(s) 289
BglI GCCNNNNNGGC 1 cut(s) 43
BisI GCNGC 9 cut(s) 6, 47, 50, 58, 69, 166, 298, 473, 542
BlsI GCNGC 9 cut(s) 7, 48, 51, 59, 70, 167, 299, 474, 543
Bme1390I CCNGG 2 cut(s) 155, 856
Bme18I GGWCC 4 cut(s) 319, 376, 419, 557
BmeRI GACNNNNNGTC 1 cut(s) 325
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 4 cut(s) 319, 376, 419, 557
BmiI GGNNCC 1 cut(s) 860
BmrFI CCNGG 2 cut(s) 155, 856
BmsI GCATC 3 cut(s) 382, 490, 811
BoxI GACNNNNGTC 1 cut(s) 146
Bpu14I TTCGAA 1 cut(s) 719
BsaAI YACGTR 1 cut(s) 261
BsaHI GRCGYC 2 cut(s) 168, 213
BsaI GGTCTC 1 cut(s) 194
BsaWI WCCGGW 3 cut(s) 103, 421, 559
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 1 cut(s) 225
Bse3DI GCAATG 2 cut(s) 38, 52
BseAI TCCGGA 1 cut(s) 559
BseBI CCWGG 2 cut(s) 155, 856
BseGI GGATG 4 cut(s) 397, 430, 484, 505
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 2 cut(s) 38, 52
BseMII CTCAG 2 cut(s) 17, 205
BseNI ACTGG 1 cut(s) 225
BseRI GAGGAG 1 cut(s) 635
BseXI GCAGC 5 cut(s) 44, 58, 61, 459, 528
Bsh1236I CGCG 4 cut(s) 165, 186, 300, 326
BshFI GGCC 3 cut(s) 37, 158, 488
BsiSI CCGG 3 cut(s) 104, 422, 560
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 3 cut(s) 194, 557, 884
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 3 cut(s) 37, 158, 488
Bso31I GGTCTC 1 cut(s) 194
Bsp119I TTCGAA 1 cut(s) 719
Bsp13I TCCGGA 1 cut(s) 559
Bsp143I GATC 3 cut(s) 399, 675, 693
BspACI CCGC 8 cut(s) 14, 69, 165, 186, 295, 298, 475, 602
BspANI GGCC 3 cut(s) 37, 158, 488
BspCNI CTCAG 2 cut(s) 18, 204
BspEI TCCGGA 1 cut(s) 559
BspFNI CGCG 4 cut(s) 165, 186, 300, 326
BspLI GGNNCC 1 cut(s) 860
BspPI GGATC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 719
BspTNI GGTCTC 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 38, 52
BsrI ACTGG 1 cut(s) 225
BssMI GATC 3 cut(s) 399, 675, 693
BssNI GRCGYC 2 cut(s) 168, 213
Bst2UI CCWGG 2 cut(s) 155, 856
Bst4CI ACNGT 3 cut(s) 148, 198, 737
Bst6I CTCTTC 2 cut(s) 591, 624
BstACI GRCGYC 2 cut(s) 168, 213
BstBAI YACGTR 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 719
BstC8I GCNNGC 2 cut(s) 295, 546
BstDEI CTNAG 3 cut(s) 26, 191, 746
BstEII GGTNACC 1 cut(s) 106
BstF5I GGATG 4 cut(s) 397, 430, 484, 505
BstFNI CGCG 4 cut(s) 165, 186, 300, 326
BstH2I RGCGCY 1 cut(s) 289
BstHHI GCGC 3 cut(s) 288, 302, 354
BstKTI GATC 3 cut(s) 402, 678, 696
BstMAI GTCTC 3 cut(s) 194, 557, 884
BstMBI GATC 3 cut(s) 399, 675, 693
BstMWI GCNNNNNNNGC 7 cut(s) 11, 28, 43, 221, 294, 299, 845
BstNI CCWGG 2 cut(s) 155, 856
BstPAI GACNNNNGTC 1 cut(s) 146
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 2 cut(s) 153, 854
BstUI CGCG 4 cut(s) 165, 186, 300, 326
BstV1I GCAGC 5 cut(s) 44, 58, 61, 459, 528
BsuRI GGCC 3 cut(s) 37, 158, 488
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 4 cut(s) 397, 430, 484, 505
BtsI GCAGTG 1 cut(s) 831
BtsIMutI CAGTG 1 cut(s) 831
Cac8I GCNNGC 2 cut(s) 295, 546
CfoI GCGC 3 cut(s) 288, 302, 354
Cfr13I GGNCC 4 cut(s) 319, 376, 419, 557
CseI GACGC 3 cut(s) 157, 221, 315
CsiI ACCWGGT 1 cut(s) 854
Csp6I GTAC 1 cut(s) 262
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 26, 191, 746
DpnI GATC 3 cut(s) 401, 677, 695
DpnII GATC 3 cut(s) 399, 675, 693
DriI GACNNNNNGTC 1 cut(s) 325
EaeI YGGCCR 2 cut(s) 35, 156
Eam1104I CTCTTC 2 cut(s) 591, 624
Eam1105I GACNNNNNGTC 1 cut(s) 325
EarI CTCTTC 2 cut(s) 591, 624
EciI GGCGGA 1 cut(s) 617
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 4 cut(s) 319, 376, 419, 557
Eco91I GGTNACC 1 cut(s) 106
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 153, 854
FaiI YATR 5 cut(s) 174, 317, 459, 899, 901
FaqI GGGAC 1 cut(s) 30
FauI CCCGC 3 cut(s) 7, 302, 468
FbaI TGATCA 1 cut(s) 675
Fnu4HI GCNGC 9 cut(s) 6, 47, 50, 58, 69, 166, 298, 473, 542
FokI GGATG 4 cut(s) 404, 437, 491, 512
Fsp4HI GCNGC 9 cut(s) 6, 47, 50, 58, 69, 166, 298, 473, 542
FspBI CTAG 3 cut(s) 404, 774, 887
GlaI GCGC 3 cut(s) 287, 301, 353
GluI GCNGC 9 cut(s) 6, 47, 50, 58, 69, 166, 298, 473, 542
HaeII RGCGCY 1 cut(s) 289
HaeIII GGCC 3 cut(s) 37, 158, 488
HapII CCGG 3 cut(s) 104, 422, 560
HgaI GACGC 3 cut(s) 157, 221, 315
HhaI GCGC 3 cut(s) 288, 302, 354
Hin1I GRCGYC 2 cut(s) 168, 213
Hin6I GCGC 3 cut(s) 286, 300, 352
HinP1I GCGC 3 cut(s) 286, 300, 352
HindIII AAGCTT 1 cut(s) 546
HinfI GANTC 3 cut(s) 245, 251, 508
HpaII CCGG 3 cut(s) 104, 422, 560
HphI GGTGA 3 cut(s) 100, 688, 844
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 7 cut(s) 194, 202, 244, 250, 362, 675, 761
Hpy188III TCNNGA 5 cut(s) 374, 431, 560, 740, 774
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 2 cut(s) 132, 533
HpyAV CCTTC 6 cut(s) 82, 340, 428, 438, 781, 872
HpyCH4III ACNGT 3 cut(s) 148, 198, 737
HpyCH4IV ACGT 3 cut(s) 260, 384, 880
HpyCH4V TGCA 3 cut(s) 544, 769, 824
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 28, 43, 221, 294, 299, 845
HpyF3I CTNAG 3 cut(s) 26, 191, 746
HpySE526I ACGT 3 cut(s) 260, 384, 880
Hsp92I GRCGYC 2 cut(s) 168, 213
HspAI GCGC 3 cut(s) 286, 300, 352
Kpn2I TCCGGA 1 cut(s) 559
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 3 cut(s) 399, 675, 693
Lsp1109I GCAGC 5 cut(s) 44, 58, 61, 459, 528
LweI GCATC 3 cut(s) 382, 490, 811
MabI ACCWGGT 1 cut(s) 854
MaeI CTAG 3 cut(s) 404, 774, 887
MaeII ACGT 3 cut(s) 260, 384, 880
MaeIII GTNAC 3 cut(s) 106, 256, 272
MalI GATC 3 cut(s) 401, 677, 695
MboI GATC 3 cut(s) 399, 675, 693
MboII GAAGA 6 cut(s) 79, 421, 608, 617, 638, 641
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 3 cut(s) 413, 454, 714
MluNI TGGCCA 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 158
MroI TCCGGA 1 cut(s) 559
MscI TGGCCA 1 cut(s) 158
MseI TTAA 1 cut(s) 690
MslI CAYNNNNRTG 1 cut(s) 645
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 3 cut(s) 104, 422, 560
MspR9I CCNGG 2 cut(s) 155, 856
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 2 cut(s) 155, 856
MvnI CGCG 4 cut(s) 165, 186, 300, 326
MwoI GCNNNNNNNGC 7 cut(s) 11, 28, 43, 221, 294, 299, 845
NdeII GATC 3 cut(s) 399, 675, 693
NlaIV GGNNCC 1 cut(s) 860
NmuCI GTSAC 2 cut(s) 106, 256
NspV TTCGAA 1 cut(s) 719
PctI GAATGC 1 cut(s) 769
PfeI GAWTC 3 cut(s) 245, 251, 508
PkrI GCNGC 9 cut(s) 7, 48, 51, 59, 70, 167, 299, 474, 543
Ppu21I YACGTR 1 cut(s) 261
PshAI GACNNNNGTC 1 cut(s) 146
Psp6I CCWGG 2 cut(s) 153, 854
PspEI GGTNACC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 153, 854
PspN4I GGNNCC 1 cut(s) 860
PspPI GGNCC 4 cut(s) 319, 376, 419, 557
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 645
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 9 cut(s) 6, 47, 50, 58, 69, 166, 298, 473, 542
Sau3AI GATC 3 cut(s) 399, 675, 693
Sau96I GGNCC 4 cut(s) 319, 376, 419, 557
ScrFI CCNGG 2 cut(s) 155, 856
SexAI ACCWGGT 1 cut(s) 854
SfaNI GCATC 3 cut(s) 382, 490, 811
SfuI TTCGAA 1 cut(s) 719
SinI GGWCC 4 cut(s) 319, 376, 419, 557
SmiMI CAYNNNNRTG 1 cut(s) 645
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
Sse9I AATT 3 cut(s) 413, 454, 714
SsiI CCGC 8 cut(s) 14, 69, 165, 186, 295, 298, 475, 602
SspMI CTAG 3 cut(s) 404, 774, 887
StyD4I CCNGG 2 cut(s) 153, 854
TaaI ACNGT 3 cut(s) 148, 198, 737
TaiI ACGT 3 cut(s) 263, 387, 883
TaqI TCGA 2 cut(s) 430, 719
TasI AATT 3 cut(s) 413, 454, 714
TauI GCSGC 3 cut(s) 71, 168, 300
TfiI GAWTC 3 cut(s) 245, 251, 508
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 2 cut(s) 106, 256
TseI GCWGC 6 cut(s) 5, 46, 49, 57, 472, 541
Tsp45I GTSAC 2 cut(s) 106, 256
TspDTI ATGAA 2 cut(s) 663, 717
TspGWI ACGGA 2 cut(s) 123, 296
TspRI CASTG 1 cut(s) 831
VpaK11BI GGWCC 4 cut(s) 319, 376, 419, 557
XbaI TCTAGA 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XspI CTAG 3 cut(s) 404, 774, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.