MD08G1011800.v1.1

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011405
Physical Location & Seq
Reverse (-)
889238 .. 892237
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1011800.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGACCTCGCCTCCTCCACCACCCCTCAAATCAAACGCATTGTCAGAGTGGCTTCTCTGGACCAAGACGTGTTGGATGCTGTGATCCTAGTGAAGGTGTTTGTGTTAATGCCGCAAAAACTAGGACCGGATGTTCGAGAAGGGAAGCGAAGGCAGTTCAATTATGTTGGGAAACTGCTGCGGGATGTGGAGGCCGACTTGATGGATGCTTTGATTCAGGCTACAAAGGAGAGCGACGAAAGTAAGCTGCAAGCTTTATTAGGTCCGGAGACATTGGGGGTTCATGATGTTGCCAACGAAGAAGAAGCGGAAGAAACTGATTTTGAGGAGGAAGAAGAGGGTTCTCACGTTGATGACGCAACCAGATGGTTTGATGGTCTGATCAGTGAGGACATTAAGATCACCAATGAAGTATATTCAATTTCGAGTGTTGATTTTGACTGTCAGGAACTATGGAAACTTGTCCGAAGTGTGCATTCTAGATTGGAACAGAAGGTTGATTTGGAGGAAAATACGGGAAAGATTGATGCAGTGAGAGCGAGTGCTAAAAAGGCACTCACCAGGTTCCTTCGTTCCCTTGCTAAACGTAGCCTAGAGATTGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

203

Amino Acids

22.81

Weight (kDa)

4.67

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 40 - 191 6.6e-15 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 265
AciI CCGC 3 cut(s) 113, 181, 308
AclWI GGATC 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 94
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 2 cut(s) 160, 420
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 560
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AloI GAACNNNNNNTCC 2 cut(s) 117, 149
AluBI AGCT 2 cut(s) 247, 254
AluI AGCT 2 cut(s) 247, 254
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 79
Aor13HI TCCGGA 1 cut(s) 265
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 2 cut(s) 178, 247
ArsI GACNNNNNNTTYG 4 cut(s) 27, 59, 117, 149
AspS9I GGNCC 4 cut(s) 4, 61, 125, 263
AsuHPI GGTGA 2 cut(s) 394, 550
AvaII GGWCC 4 cut(s) 4, 61, 125, 263
BbvI GCAGC 2 cut(s) 165, 234
BccI CCATC 3 cut(s) 196, 360, 368
BciT130I CCWGG 1 cut(s) 562
BclI TGATCA 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 4 cut(s) 89, 122, 480, 593
BisI GCNGC 3 cut(s) 113, 179, 248
BlsI GCNGC 3 cut(s) 114, 180, 249
Bme1390I CCNGG 1 cut(s) 562
Bme18I GGWCC 4 cut(s) 4, 61, 125, 263
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 4 cut(s) 4, 61, 125, 263
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 1 cut(s) 562
BmsI GCATC 3 cut(s) 67, 196, 517
BsaWI WCCGGW 2 cut(s) 127, 265
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 94
BseAI TCCGGA 1 cut(s) 265
BseBI CCWGG 1 cut(s) 562
BseGI GGATG 4 cut(s) 82, 136, 190, 211
BseLI CCNNNNNNNGG 1 cut(s) 94
BseRI GAGGAG 2 cut(s) 4, 341
BseXI GCAGC 2 cut(s) 165, 234
BshFI GGCC 1 cut(s) 194
BsiSI CCGG 2 cut(s) 128, 266
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 263
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 1 cut(s) 194
Bsp13I TCCGGA 1 cut(s) 265
Bsp143I GATC 3 cut(s) 84, 381, 399
BspACI CCGC 3 cut(s) 113, 181, 308
BspANI GGCC 1 cut(s) 194
BspEI TCCGGA 1 cut(s) 265
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 566
BspPI GGATC 1 cut(s) 79
BssMI GATC 3 cut(s) 84, 381, 399
Bst2UI CCWGG 1 cut(s) 562
Bst4CI ACNGT 1 cut(s) 443
Bst6I CTCTTC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 252
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 4 cut(s) 82, 136, 190, 211
BstKTI GATC 3 cut(s) 87, 384, 402
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 3 cut(s) 84, 381, 399
BstMWI GCNNNNNNNGC 2 cut(s) 536, 551
BstNI CCWGG 1 cut(s) 562
BstSCI CCNGG 1 cut(s) 560
BstV1I GCAGC 2 cut(s) 165, 234
BsuRI GGCC 1 cut(s) 194
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 4 cut(s) 82, 136, 190, 211
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 2 cut(s) 391, 537
Cac8I GCNNGC 1 cut(s) 252
CciI TCATGA 1 cut(s) 283
Cfr13I GGNCC 4 cut(s) 4, 61, 125, 263
CseI GACGC 1 cut(s) 365
CsiI ACCWGGT 1 cut(s) 560
CviAII CATG 1 cut(s) 284
CviJI RGCY 6 cut(s) 53, 194, 221, 247, 254, 591
CviKI_1 RGCY 6 cut(s) 53, 194, 221, 247, 254, 591
DpnI GATC 3 cut(s) 86, 383, 401
DpnII GATC 3 cut(s) 84, 381, 399
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco47I GGWCC 4 cut(s) 4, 61, 125, 263
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 560
FaeI CATG 1 cut(s) 287
FaiI YATR 6 cut(s) 165, 285, 415, 454, 605, 607
FatI CATG 1 cut(s) 283
FauI CCCGC 1 cut(s) 174
FbaI TGATCA 1 cut(s) 381
Fnu4HI GCNGC 3 cut(s) 113, 179, 248
FokI GGATG 4 cut(s) 89, 143, 197, 218
Fsp4HI GCNGC 3 cut(s) 113, 179, 248
FspBI CTAG 4 cut(s) 89, 122, 480, 593
GluI GCNGC 3 cut(s) 113, 179, 248
HaeIII GGCC 1 cut(s) 194
HapII CCGG 2 cut(s) 128, 266
HgaI GACGC 1 cut(s) 365
Hin1II CATG 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 1 cut(s) 214
HpaII CCGG 2 cut(s) 128, 266
HphI GGTGA 2 cut(s) 394, 550
Hpy188I TCNGA 3 cut(s) 47, 381, 467
Hpy188III TCNNGA 6 cut(s) 59, 137, 266, 284, 446, 480
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 5 cut(s) 88, 134, 144, 487, 578
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4IV ACGT 3 cut(s) 69, 348, 586
HpyCH4V TGCA 3 cut(s) 250, 475, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 551
HpySE526I ACGT 3 cut(s) 69, 348, 586
Hsp92II CATG 1 cut(s) 287
Kpn2I TCCGGA 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 3 cut(s) 84, 381, 399
LpnPI CCDG 8 cut(s) 44, 141, 203, 279, 376, 431, 547, 574
Lsp1109I GCAGC 2 cut(s) 165, 234
LweI GCATC 3 cut(s) 67, 196, 517
MabI ACCWGGT 1 cut(s) 560
MaeI CTAG 4 cut(s) 89, 122, 480, 593
MaeII ACGT 3 cut(s) 69, 348, 586
MalI GATC 3 cut(s) 86, 383, 401
MboI GATC 3 cut(s) 84, 381, 399
MboII GAAGA 5 cut(s) 311, 314, 323, 344, 347
MluCI AATT 2 cut(s) 160, 420
MmeI TCCRAC 1 cut(s) 54
MroI TCCGGA 1 cut(s) 265
MseI TTAA 2 cut(s) 107, 396
MslI CAYNNNNRTG 1 cut(s) 351
MspI CCGG 2 cut(s) 128, 266
MspR9I CCNGG 1 cut(s) 562
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 1 cut(s) 562
MwoI GCNNNNNNNGC 2 cut(s) 536, 551
NdeII GATC 3 cut(s) 84, 381, 399
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 1 cut(s) 566
PagI TCATGA 1 cut(s) 283
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 1 cut(s) 214
PkrI GCNGC 3 cut(s) 114, 180, 249
Psp6I CCWGG 1 cut(s) 560
PspGI CCWGG 1 cut(s) 560
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 4 cut(s) 4, 61, 125, 263
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 2 cut(s) 107, 396
SatI GCNGC 3 cut(s) 113, 179, 248
Sau3AI GATC 3 cut(s) 84, 381, 399
Sau96I GGNCC 4 cut(s) 4, 61, 125, 263
ScrFI CCNGG 1 cut(s) 562
SexAI ACCWGGT 1 cut(s) 560
SfaNI GCATC 3 cut(s) 67, 196, 517
SinI GGWCC 4 cut(s) 4, 61, 125, 263
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 2 cut(s) 160, 420
SsiI CCGC 3 cut(s) 113, 181, 308
SspMI CTAG 4 cut(s) 89, 122, 480, 593
StyD4I CCNGG 1 cut(s) 560
TaaI ACNGT 1 cut(s) 443
TaiI ACGT 3 cut(s) 72, 351, 589
TaqI TCGA 2 cut(s) 136, 425
TasI AATT 2 cut(s) 160, 420
TauI GCSGC 1 cut(s) 115
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 2 cut(s) 107, 396
Tru9I TTAA 2 cut(s) 107, 396
TscAI CASTG 2 cut(s) 391, 537
TseI GCWGC 2 cut(s) 178, 247
TspDTI ATGAA 2 cut(s) 272, 423
TspRI CASTG 2 cut(s) 391, 537
VpaK11BI GGWCC 4 cut(s) 4, 61, 125, 263
XagI CCTNNNNNAGG 1 cut(s) 92
XbaI TCTAGA 1 cut(s) 479
XspI CTAG 4 cut(s) 89, 122, 480, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.