Rroxscaffold_7G00165100

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011405
Physical Location & Seq
Reverse (-)
6990691 .. 6995602
4912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00165100.1

Sequence Viewer

Length: 912 bp
ATGGCTCAACTGGGAAGACTGCTGGTAAGGCAATGGCCGGCGTTGCAGCACCACTGCTGCTCCTGCGTCACACTCAATCATCTCTTCTTCTCAACCTCACTTCCACTAAGCACAAACCCTAATTCCCGCCGCTCTTCTTCCCCGGTAATCTACTTAGCTACTCGCGGCGTCCATAAACTAGTCCGGGGACTCCGACCGCGCGATGCTCCTCCGGCACCGTCCGATCTCGACGGTAACAGCAGCGGTTCCGATTCGGAGTCGTCGATTACATATAAGAGCCGCAACGCCTTGAAACGCGACGCTCGGCGCGCCGTCCGGTGGGCTATGGACCTCGCGTCCTTCTCTACACCTCAGCTCAAATTGATTATCAGAGTGGCTTCTCTGGACCAAGACGTATTGGATGCTGTTATGTTAGTGAAGAGATTTGGGAATGATGTGCGAGAAGGAAAGCGAAGGCAGTTCAATTATATTGCAAAATTGCTACGAGATGCGGACCCTGAGTTGATGGATGCTTTGATTCGCGCTACAAAAGACTGCGACCAAAAGAAGTTGCAAGATTTATGTGGTTCGGAGGCATTGAGCATTGACGATGAGGAAGAAGAAGAAGAAGAAGCTGAAGAATCTGATAACGAGGAAGAAGAGGGTTCTCACATTGATGTAGCAACCAGATGGGTTGAGGGTATGATCAATAAGGACGCTAATATCACCAATGAAGTTTATTCAATTTCAAATGTTGAGTTTGACCGTCAGGAATTGCGGAAACTGGTCCGGAAAGTACATTCAGCTTTGGAACGCTCAAGCAATTCTGAGGAAAATAAGGAAAAGATTGATGAAGCGGTAGTGGGTGCCAAAAAGTCACTCACCAGGTTCCTACGTGCCCTTGCTAAAAGCAGCCTAATGATTACTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

303

Amino Acids

34.14

Weight (kDa)

5.97

Isoelectric Point (pI)

63.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 91 - 273 2.8e-26 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 214, 845
AccBSI CCGCTC 1 cut(s) 132
AccII CGCG 7 cut(s) 165, 199, 201, 297, 309, 335, 522
AccIII TCCGGA 1 cut(s) 768
AciI CCGC 9 cut(s) 127, 130, 165, 197, 243, 280, 491, 757, 836
AcoI YGGCCR 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 1 cut(s) 318
AgsI TTSAA 4 cut(s) 292, 463, 723, 729
AhdI GACNNNNNGTC 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 178
AjnI CCWGG 1 cut(s) 863
AluBI AGCT 4 cut(s) 158, 355, 614, 785
AluI AGCT 4 cut(s) 158, 355, 614, 785
Aor13HI TCCGGA 1 cut(s) 768
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 4 cut(s) 46, 57, 240, 891
AscI GGCGCGCC 1 cut(s) 307
AspLEI GCGC 4 cut(s) 201, 309, 311, 524
AspS9I GGNCC 4 cut(s) 328, 385, 493, 766
AsuC2I CCSGG 2 cut(s) 143, 185
AsuHPI GGTGA 2 cut(s) 697, 853
AvaII GGWCC 4 cut(s) 328, 385, 493, 766
BaeGI GKGCMC 1 cut(s) 880
BanI GGYRCC 2 cut(s) 214, 845
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 44, 58, 252, 903
BccI CCATC 2 cut(s) 499, 663
BceAI ACGGC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 865
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 2 cut(s) 143, 185
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BisI GCNGC 7 cut(s) 47, 58, 130, 166, 241, 280, 892
BlsI GCNGC 7 cut(s) 48, 59, 131, 167, 242, 281, 893
Bme1390I CCNGG 3 cut(s) 143, 185, 865
Bme18I GGWCC 4 cut(s) 328, 385, 493, 766
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 4 cut(s) 328, 385, 493, 766
BmiI GGNNCC 5 cut(s) 216, 247, 495, 847, 869
BmrFI CCNGG 3 cut(s) 143, 185, 865
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 4 cut(s) 193, 391, 478, 499
BmuI ACTGGG 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 781
BpuMI CCSGG 2 cut(s) 143, 185
BsaAI YACGTR 1 cut(s) 875
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 141, 184
BsaWI WCCGGW 2 cut(s) 315, 768
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 15, 768
Bse3DI GCAATG 1 cut(s) 38
BseAI TCCGGA 1 cut(s) 768
BseBI CCWGG 1 cut(s) 865
BseDI CCNNGG 2 cut(s) 141, 184
BseGI GGATG 2 cut(s) 406, 514
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 365, 489, 798
BseNI ACTGG 2 cut(s) 15, 768
BsePI GCGCGC 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 198
BseSI GKGCMC 1 cut(s) 880
BseXI GCAGC 4 cut(s) 44, 58, 252, 903
Bsh1236I CGCG 7 cut(s) 165, 199, 201, 297, 309, 335, 522
Bsh1285I CGRYCG 1 cut(s) 197
BshFI GGCC 1 cut(s) 37
BshNI GGYRCC 2 cut(s) 214, 845
BsiEI CGRYCG 1 cut(s) 197
BsiSI CCGG 6 cut(s) 38, 143, 184, 212, 316, 769
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 880
Bsp13I TCCGGA 1 cut(s) 768
Bsp143I GATC 2 cut(s) 223, 684
BspACI CCGC 9 cut(s) 127, 130, 165, 197, 243, 280, 491, 757, 836
BspANI GGCC 1 cut(s) 37
BspCNI CTCAG 3 cut(s) 364, 490, 799
BspEI TCCGGA 1 cut(s) 768
BspFNI CGCG 7 cut(s) 165, 199, 201, 297, 309, 335, 522
BspLI GGNNCC 5 cut(s) 216, 247, 495, 847, 869
BspQI GCTCTTC 1 cut(s) 139
BspT107I GGYRCC 2 cut(s) 214, 845
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 2 cut(s) 15, 768
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 141, 184
BssHII GCGCGC 1 cut(s) 307
BssMI GATC 2 cut(s) 223, 684
BssNI GRCGYC 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 865
Bst4CI ACNGT 3 cut(s) 219, 233, 746
Bst6I CTCTTC 4 cut(s) 89, 139, 413, 633
BstACI GRCGYC 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 875
BstC8I GCNNGC 2 cut(s) 39, 309
BstDEI CTNAG 5 cut(s) 107, 154, 351, 498, 807
BstF5I GGATG 2 cut(s) 406, 514
BstFNI CGCG 7 cut(s) 165, 199, 201, 297, 309, 335, 522
BstHHI GCGC 4 cut(s) 201, 309, 311, 524
BstKTI GATC 2 cut(s) 226, 687
BstMBI GATC 2 cut(s) 223, 684
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 5 cut(s) 28, 43, 63, 212, 308
BstNI CCWGG 1 cut(s) 865
BstSCI CCNGG 3 cut(s) 141, 183, 863
BstSLI GKGCMC 1 cut(s) 880
BstUI CGCG 7 cut(s) 165, 199, 201, 297, 309, 335, 522
BstV1I GCAGC 4 cut(s) 44, 58, 252, 903
BstV2I GAAGAC 1 cut(s) 22
BsuRI GGCC 1 cut(s) 37
BtgZI GCGATG 1 cut(s) 216
BtsCI GGATG 2 cut(s) 406, 514
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 2 cut(s) 39, 309
CfoI GCGC 4 cut(s) 201, 309, 311, 524
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 4 cut(s) 328, 385, 493, 766
CseI GACGC 5 cut(s) 55, 157, 308, 324, 704
CsiI ACCWGGT 1 cut(s) 863
Csp6I GTAC 1 cut(s) 776
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 5 cut(s) 107, 154, 351, 498, 807
DpnI GATC 2 cut(s) 225, 686
DpnII GATC 2 cut(s) 223, 684
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 35
Eam1104I CTCTTC 4 cut(s) 89, 139, 413, 633
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 4 cut(s) 89, 139, 413, 633
Eco47I GGWCC 4 cut(s) 328, 385, 493, 766
Eco57I CTGAAG 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 863
FaiI YATR 9 cut(s) 174, 271, 273, 326, 410, 468, 562, 683, 910
FaqI GGGAC 1 cut(s) 201
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 7 cut(s) 47, 58, 130, 166, 241, 280, 892
FokI GGATG 2 cut(s) 413, 521
Fsp4HI GCNGC 7 cut(s) 47, 58, 130, 166, 241, 280, 892
FspBI CTAG 1 cut(s) 179
GlaI GCGC 4 cut(s) 200, 308, 310, 523
GluI GCNGC 7 cut(s) 47, 58, 130, 166, 241, 280, 892
HaeIII GGCC 1 cut(s) 37
HapII CCGG 6 cut(s) 38, 143, 184, 212, 316, 769
HgaI GACGC 5 cut(s) 55, 157, 308, 324, 704
HhaI GCGC 4 cut(s) 201, 309, 311, 524
Hin1I GRCGYC 1 cut(s) 168
Hin6I GCGC 4 cut(s) 199, 307, 309, 522
HinP1I GCGC 4 cut(s) 199, 307, 309, 522
HinfI GANTC 5 cut(s) 189, 251, 257, 517, 620
HpaII CCGG 6 cut(s) 38, 143, 184, 212, 316, 769
HphI GGTGA 2 cut(s) 697, 853
Hpy188I TCNGA 8 cut(s) 194, 223, 250, 256, 371, 571, 625, 808
Hpy188III TCNNGA 4 cut(s) 227, 383, 749, 769
Hpy99I CGWCG 3 cut(s) 233, 265, 302
HpyAV CCTTC 3 cut(s) 349, 437, 447
HpyCH4III ACNGT 3 cut(s) 219, 233, 746
HpyCH4IV ACGT 2 cut(s) 393, 874
HpyCH4V TGCA 3 cut(s) 46, 473, 553
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 43, 63, 212, 308
HpyF3I CTNAG 5 cut(s) 107, 154, 351, 498, 807
HpySE526I ACGT 2 cut(s) 393, 874
Hsp92I GRCGYC 1 cut(s) 168
HspAI GCGC 4 cut(s) 199, 307, 309, 522
Kpn2I TCCGGA 1 cut(s) 768
KroI GCCGGC 1 cut(s) 37
KroNI GCCGGC 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 2 cut(s) 223, 684
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 2 cut(s) 65, 211
Lsp1109I GCAGC 4 cut(s) 44, 58, 252, 903
LweI GCATC 4 cut(s) 193, 391, 478, 499
MabI ACCWGGT 1 cut(s) 863
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 393, 874
MaeIII GTNAC 3 cut(s) 67, 233, 855
MalI GATC 2 cut(s) 225, 686
MbiI CCGCTC 1 cut(s) 132
MboI GATC 2 cut(s) 223, 684
MhlI GDGCHC 1 cut(s) 880
MluCI AATT 7 cut(s) 121, 359, 463, 476, 723, 752, 802
MlyI GAGTC 2 cut(s) 183, 266
MmeI TCCRAC 1 cut(s) 217
MroI TCCGGA 1 cut(s) 768
MroNI GCCGGC 1 cut(s) 37
MslI CAYNNNNRTG 1 cut(s) 654
MspA1I CMGCKG 1 cut(s) 243
MspI CCGG 6 cut(s) 38, 143, 184, 212, 316, 769
MspR9I CCNGG 3 cut(s) 143, 185, 865
MvaI CCWGG 1 cut(s) 865
MvnI CGCG 7 cut(s) 165, 199, 201, 297, 309, 335, 522
MwoI GCNNNNNNNGC 5 cut(s) 28, 43, 63, 212, 308
NaeI GCCGGC 1 cut(s) 39
NciI CCSGG 2 cut(s) 143, 185
NdeII GATC 2 cut(s) 223, 684
NgoMIV GCCGGC 1 cut(s) 37
NlaIV GGNNCC 5 cut(s) 216, 247, 495, 847, 869
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 2 cut(s) 67, 855
PalAI GGCGCGCC 1 cut(s) 307
PauI GCGCGC 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PdiI GCCGGC 1 cut(s) 39
PfeI GAWTC 3 cut(s) 251, 517, 620
PkrI GCNGC 7 cut(s) 48, 59, 131, 167, 242, 281, 893
PleI GAGTC 2 cut(s) 183, 265
PpsI GAGTC 2 cut(s) 183, 265
Ppu21I YACGTR 1 cut(s) 875
Psp6I CCWGG 1 cut(s) 863
PspGI CCWGG 1 cut(s) 863
PspN4I GGNNCC 5 cut(s) 216, 247, 495, 847, 869
PspPI GGNCC 4 cut(s) 328, 385, 493, 766
PteI GCGCGC 1 cut(s) 307
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
RseI CAYNNNNRTG 1 cut(s) 654
SapI GCTCTTC 1 cut(s) 139
SatI GCNGC 7 cut(s) 47, 58, 130, 166, 241, 280, 892
Sau3AI GATC 2 cut(s) 223, 684
Sau96I GGNCC 4 cut(s) 328, 385, 493, 766
SchI GAGTC 2 cut(s) 183, 266
ScrFI CCNGG 3 cut(s) 143, 185, 865
SduI GDGCHC 1 cut(s) 880
SexAI ACCWGGT 1 cut(s) 863
SfaNI GCATC 4 cut(s) 193, 391, 478, 499
SgsI GGCGCGCC 1 cut(s) 307
SinI GGWCC 4 cut(s) 328, 385, 493, 766
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 1 cut(s) 796
SmoI CTYRAG 1 cut(s) 796
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 7 cut(s) 121, 359, 463, 476, 723, 752, 802
SsiI CCGC 9 cut(s) 127, 130, 165, 197, 243, 280, 491, 757, 836
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 3 cut(s) 141, 183, 863
TaaI ACNGT 3 cut(s) 219, 233, 746
TaiI ACGT 2 cut(s) 396, 877
TaqI TCGA 2 cut(s) 228, 263
TasI AATT 7 cut(s) 121, 359, 463, 476, 723, 752, 802
TatI WGTACW 1 cut(s) 775
TauI GCSGC 3 cut(s) 132, 168, 282
TfiI GAWTC 3 cut(s) 251, 517, 620
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 2 cut(s) 67, 855
TseI GCWGC 4 cut(s) 46, 57, 240, 891
Tsp45I GTSAC 2 cut(s) 67, 855
TspDTI ATGAA 2 cut(s) 726, 846
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 4 cut(s) 328, 385, 493, 766
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.