Rh6BG470200

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011405
Physical Location & Seq
Reverse (-)
67818719 .. 67822840
4122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG470200.1

Sequence Viewer

Length: 804 bp
ATGGCTCAACTGGGAAGACTGCTGGTAAGGCAATGCCCGGCGTTGCAGCACCACTGCTGCTCCTGCGTCACACTCAATCATCTCTTCTTCTCAACCTCACTTCCACTAAGCACAAACCCTAATTCCCGCCGCTCTTCTTCCCCGGTAATCTACTTAGCTACTCGCGGCGTCCATAAACTAGTCCGCGGACTCCGACCGCGCGACGCTCCTCCGGCACCGTCCGATCTCGACGGTAACAGCAGCGGTTCCGATTCGGAGTCGTCGATTACATATAAGAGCCGCAACGCCTTGAAACGCGACGCTCGGCGCGCCGTCCGGTGGGCTATGGACCTCGCGTCCTTCTCTACACCTCAGCTCAAATTGATTATCAGAGTGGCTTCTCTGGACCAAGACGTATTGGATGCTGTTATGTTAGTGAAGAGATTTGGGAATGATGTGCGAGAAGGAAAGCGAAGGCAGTTCAATTATATTGCAAAATTGCTACGAGATGCGGACCCTGAGTTGATGGATGCTTTGATTCGCGCTACAAAAGACTGCGACCAAAAGAAGTTGCGAGATTTATGTGGTTCGGAGGCGTTGAGCATTGACGATGAGGAAGAAGAAGAAGAAGAAGAAGCTGAAGAATCTGATAACGAGGAAGAAGAGGGTTCTCACATTGATGTAGCAACCAGATGGGTTGAGGGTATGATCAATAAGGACGCTAATATCACCAATGAAGTTTATTCAATTTCAAATGTTGAGTTTGACCGTCAGGAAGCATTGCATTCTGTATCTTGGCTGTTTGCTAATGTCAGCCTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

267

Amino Acids

30.01

Weight (kDa)

5.19

Isoelectric Point (pI)

65.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 91 - 252 7.2e-21 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 132
AccII CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
AciI CCGC 9 cut(s) 127, 130, 165, 184, 186, 197, 243, 280, 491
AcuI CTGAAG 1 cut(s) 639
AcyI GRCGYC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 318
AgsI TTSAA 4 cut(s) 292, 463, 726, 732
AhdI GACNNNNNGTC 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 178
AluBI AGCT 3 cut(s) 158, 355, 617
AluI AGCT 3 cut(s) 158, 355, 617
ApeKI GCWGC 3 cut(s) 46, 57, 240
AscI GGCGCGCC 1 cut(s) 307
AspLEI GCGC 4 cut(s) 201, 309, 311, 524
AspS9I GGNCC 3 cut(s) 328, 385, 493
AsuC2I CCSGG 2 cut(s) 38, 143
AsuHPI GGTGA 1 cut(s) 700
AvaII GGWCC 3 cut(s) 328, 385, 493
BanI GGYRCC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 3 cut(s) 44, 58, 252
BccI CCATC 2 cut(s) 499, 666
BceAI ACGGC 1 cut(s) 296
BclI TGATCA 1 cut(s) 687
BcnI CCSGG 2 cut(s) 38, 143
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BisI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
BlsI GCNGC 6 cut(s) 48, 59, 131, 167, 242, 281
Bme1390I CCNGG 2 cut(s) 38, 143
Bme18I GGWCC 3 cut(s) 328, 385, 493
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 328, 385, 493
BmiI GGNNCC 3 cut(s) 216, 247, 495
BmrFI CCNGG 2 cut(s) 38, 143
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 391, 478, 499
BmuI ACTGGG 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 351
BpuMI CCSGG 2 cut(s) 38, 143
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 141, 184
BsaWI WCCGGW 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 2 cut(s) 38, 758
BseDI CCNNGG 2 cut(s) 141, 184
BseGI GGATG 2 cut(s) 406, 514
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 2 cut(s) 38, 758
BseMII CTCAG 2 cut(s) 365, 489
BseNI ACTGG 1 cut(s) 15
BsePI GCGCGC 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 3 cut(s) 44, 58, 252
Bsh1236I CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 197
BsiSI CCGG 4 cut(s) 38, 143, 212, 316
BslI CCNNNNNNNGG 1 cut(s) 318
BsmI GAATGC 1 cut(s) 763
Bsp143I GATC 2 cut(s) 223, 687
BspACI CCGC 9 cut(s) 127, 130, 165, 184, 186, 197, 243, 280, 491
BspCNI CTCAG 2 cut(s) 364, 490
BspFNI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BspLI GGNNCC 3 cut(s) 216, 247, 495
BspQI GCTCTTC 1 cut(s) 139
BspT107I GGYRCC 1 cut(s) 214
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 2 cut(s) 38, 758
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 141, 184
BssHII GCGCGC 1 cut(s) 307
BssMI GATC 2 cut(s) 223, 687
BssNI GRCGYC 1 cut(s) 168
Bst4CI ACNGT 3 cut(s) 219, 233, 749
Bst6I CTCTTC 4 cut(s) 89, 139, 413, 636
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 4 cut(s) 107, 154, 351, 498
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 406, 514
BstFNI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BstHHI GCGC 4 cut(s) 201, 309, 311, 524
BstKTI GATC 2 cut(s) 226, 690
BstMBI GATC 2 cut(s) 223, 687
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
BstSCI CCNGG 2 cut(s) 36, 141
BstUI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BstV1I GCAGC 3 cut(s) 44, 58, 252
BstV2I GAAGAC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 406, 514
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 4 cut(s) 201, 309, 311, 524
Cfr13I GGNCC 3 cut(s) 328, 385, 493
Cfr42I CCGCGG 1 cut(s) 187
CseI GACGC 6 cut(s) 55, 157, 212, 308, 324, 707
CviJI RGCY 9 cut(s) 5, 158, 279, 323, 355, 377, 617, 778, 795
CviKI_1 RGCY 9 cut(s) 5, 158, 279, 323, 355, 377, 617, 778, 795
DdeI CTNAG 4 cut(s) 107, 154, 351, 498
DpnI GATC 2 cut(s) 225, 689
DpnII GATC 2 cut(s) 223, 687
DriI GACNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 4 cut(s) 89, 139, 413, 636
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 4 cut(s) 89, 139, 413, 636
Eco47I GGWCC 3 cut(s) 328, 385, 493
Eco57I CTGAAG 1 cut(s) 639
FaiI YATR 9 cut(s) 174, 271, 273, 326, 410, 468, 562, 686, 802
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 687
Fnu4HI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
FokI GGATG 2 cut(s) 413, 521
Fsp4HI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
FspBI CTAG 1 cut(s) 179
GlaI GCGC 4 cut(s) 200, 308, 310, 523
GluI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
HapII CCGG 4 cut(s) 38, 143, 212, 316
HgaI GACGC 6 cut(s) 55, 157, 212, 308, 324, 707
HhaI GCGC 4 cut(s) 201, 309, 311, 524
Hin1I GRCGYC 1 cut(s) 168
Hin6I GCGC 4 cut(s) 199, 307, 309, 522
HinP1I GCGC 4 cut(s) 199, 307, 309, 522
HinfI GANTC 5 cut(s) 189, 251, 257, 517, 623
HpaII CCGG 4 cut(s) 38, 143, 212, 316
HphI GGTGA 1 cut(s) 700
Hpy188I TCNGA 7 cut(s) 194, 223, 250, 256, 371, 571, 628
Hpy188III TCNNGA 3 cut(s) 227, 383, 752
Hpy99I CGWCG 4 cut(s) 206, 233, 265, 302
HpyAV CCTTC 3 cut(s) 349, 437, 447
HpyCH4III ACNGT 3 cut(s) 219, 233, 749
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 3 cut(s) 46, 473, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
HpyF3I CTNAG 4 cut(s) 107, 154, 351, 498
HpySE526I ACGT 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 168
HspAI GCGC 4 cut(s) 199, 307, 309, 522
Ksp22I TGATCA 1 cut(s) 687
KspI CCGCGG 1 cut(s) 187
Kzo9I GATC 2 cut(s) 223, 687
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 2 cut(s) 65, 211
Lsp1109I GCAGC 3 cut(s) 44, 58, 252
LweI GCATC 3 cut(s) 391, 478, 499
MaeI CTAG 1 cut(s) 179
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 67, 233
MalI GATC 2 cut(s) 225, 689
MbiI CCGCTC 1 cut(s) 132
MboI GATC 2 cut(s) 223, 687
MluCI AATT 5 cut(s) 121, 359, 463, 476, 726
MlyI GAGTC 2 cut(s) 183, 266
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 9 cut(s) 106, 219, 341, 360, 565, 586, 628, 637, 673
MslI CAYNNNNRTG 1 cut(s) 657
MspA1I CMGCKG 2 cut(s) 186, 243
MspI CCGG 4 cut(s) 38, 143, 212, 316
MspR9I CCNGG 2 cut(s) 38, 143
Mva1269I GAATGC 1 cut(s) 763
MvnI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
MwoI GCNNNNNNNGC 4 cut(s) 28, 63, 212, 308
NciI CCSGG 2 cut(s) 38, 143
NdeII GATC 2 cut(s) 223, 687
NlaIV GGNNCC 3 cut(s) 216, 247, 495
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 67
PalAI GGCGCGCC 1 cut(s) 307
PauI GCGCGC 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PctI GAATGC 1 cut(s) 763
PfeI GAWTC 3 cut(s) 251, 517, 623
PkrI GCNGC 6 cut(s) 48, 59, 131, 167, 242, 281
PleI GAGTC 2 cut(s) 183, 265
PpsI GAGTC 2 cut(s) 183, 265
PspN4I GGNNCC 3 cut(s) 216, 247, 495
PspPI GGNCC 3 cut(s) 328, 385, 493
PteI GCGCGC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 657
SacII CCGCGG 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 139
SatI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
Sau3AI GATC 2 cut(s) 223, 687
Sau96I GGNCC 3 cut(s) 328, 385, 493
SchI GAGTC 2 cut(s) 183, 266
ScrFI CCNGG 2 cut(s) 38, 143
SetI ASST 7 cut(s) 98, 160, 333, 352, 357, 396, 619
SfaNI GCATC 3 cut(s) 391, 478, 499
Sfr303I CCGCGG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 187
SgsI GGCGCGCC 1 cut(s) 307
SinI GGWCC 3 cut(s) 328, 385, 493
SmiMI CAYNNNNRTG 1 cut(s) 657
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 5 cut(s) 121, 359, 463, 476, 726
SsiI CCGC 9 cut(s) 127, 130, 165, 184, 186, 197, 243, 280, 491
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 36, 141
TaaI ACNGT 3 cut(s) 219, 233, 749
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 228, 263
TasI AATT 5 cut(s) 121, 359, 463, 476, 726
TauI GCSGC 3 cut(s) 132, 168, 282
TfiI GAWTC 3 cut(s) 251, 517, 623
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 3 cut(s) 46, 57, 240
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 1 cut(s) 729
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 3 cut(s) 328, 385, 493
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.