RLG00000011114

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011405
Physical Location & Seq
Reverse (-)
6915146 .. 6917726
2581 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011114

Sequence Viewer

Length: 870 bp
ATGGCTCAACTGGGAAGACTGCTGGTAAGGCAATGCCCGGCGTTGCAGCACCACTGCTGCTCCTGCGTCACACTCAATCATCTCTTCTTCTCAACCTCACTTCCACTAAGCACAAACCCTAATTCCCGCCGCTCTTCTTCCCCGGTAATCTACTTAGCTACTCGCGGCGTCCATAAACTAGTCCGCGGACTCCGACCGCGCGACGCTCCTCCGGCACCGTCCGATCTCGACGGTAACAGCAGCGGTTCTGATTCGGAGTCGTCGATTACATATAAGAGCCGCAACGCCTTGAAACGCGACGCTCGGCGCGCCGTTCGGTGGGCTATGGACCTCGCGTCCTTCTCCACACCTCAGCTCAAATTGATTATCAGAGTGGCTTCTCTGGACCAAGACGCATTGGATGCTGTTATGTTAGTGAAGAGATTTGGGAATGATGTGCGAGAAGGAAAGCGAAGGCAGTTCAATTATATTGCAAAATTGCTACGAGATGCGGACCCTGAGTTGATGGATGCTTTGATTCGCGCTACAAAAGACTGTGACCAAAAGAAGTTGCAAGATTTATGTGGTTCGGAGGTGTTGAGCATTGATGATGAGGAAGAAGAAGAAGAAGCTGAAGAATCTGATAACGAGGAAGAAGAGGGTTCTCACATTGATGTAGCAACCAGATGGGTTGAGGGTATGATCAATAAGGACGCTAATATCACCAATGAAGTTTATTCAGTTTCAAATGTTGAGTTTGACCGTCAGGAATTGCGGAAACTGGTCCGGAAAGTACATTCAGCTTTGGAACACTCAAGCAATTCTGAGGAAAATAAGGAAAAGATTGATGAAGCGGTAGTGGGTGCCAAAAAATCTCTCACCAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

290

Amino Acids

32.44

Weight (kDa)

5.75

Isoelectric Point (pI)

63.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 91 - 272 6e-26 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 214, 842
AccBSI CCGCTC 1 cut(s) 132
AccII CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
AccIII TCCGGA 1 cut(s) 765
AcuI CTGAAG 1 cut(s) 633
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 1 cut(s) 774
AfiI CCNNNNNNNGG 1 cut(s) 318
AgsI TTSAA 3 cut(s) 292, 463, 726
AhdI GACNNNNNGTC 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 178
AjnI CCWGG 1 cut(s) 860
AluBI AGCT 4 cut(s) 158, 355, 611, 782
AluI AGCT 4 cut(s) 158, 355, 611, 782
Aor13HI TCCGGA 1 cut(s) 765
ApeKI GCWGC 3 cut(s) 46, 57, 240
AscI GGCGCGCC 1 cut(s) 307
AspLEI GCGC 4 cut(s) 201, 309, 311, 524
AspS9I GGNCC 4 cut(s) 328, 385, 493, 763
AsuC2I CCSGG 2 cut(s) 38, 143
AsuHPI GGTGA 2 cut(s) 694, 850
AvaII GGWCC 4 cut(s) 328, 385, 493, 763
BanI GGYRCC 2 cut(s) 214, 842
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 3 cut(s) 44, 58, 252
BccI CCATC 2 cut(s) 499, 660
BceAI ACGGC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 862
BclI TGATCA 1 cut(s) 681
BcnI CCSGG 2 cut(s) 38, 143
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BisI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
BlsI GCNGC 6 cut(s) 48, 59, 131, 167, 242, 281
Bme1390I CCNGG 3 cut(s) 38, 143, 862
Bme18I GGWCC 4 cut(s) 328, 385, 493, 763
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 4 cut(s) 328, 385, 493, 763
BmiI GGNNCC 3 cut(s) 216, 495, 844
BmrFI CCNGG 3 cut(s) 38, 143, 862
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 391, 478, 499
BmuI ACTGGG 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 778
BpuMI CCSGG 2 cut(s) 38, 143
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 141, 184
BsaWI WCCGGW 1 cut(s) 765
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 2 cut(s) 15, 765
Bse3DI GCAATG 1 cut(s) 38
BseAI TCCGGA 1 cut(s) 765
BseBI CCWGG 1 cut(s) 862
BseDI CCNNGG 2 cut(s) 141, 184
BseGI GGATG 2 cut(s) 406, 514
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 365, 489, 795
BseNI ACTGG 2 cut(s) 15, 765
BsePI GCGCGC 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 3 cut(s) 44, 58, 252
Bsh1236I CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 2 cut(s) 214, 842
BsiEI CGRYCG 1 cut(s) 197
BsiSI CCGG 4 cut(s) 38, 143, 212, 766
BslI CCNNNNNNNGG 1 cut(s) 318
Bsp13I TCCGGA 1 cut(s) 765
Bsp143I GATC 2 cut(s) 223, 681
BspCNI CTCAG 3 cut(s) 364, 490, 796
BspEI TCCGGA 1 cut(s) 765
BspFNI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BspLI GGNNCC 3 cut(s) 216, 495, 844
BspQI GCTCTTC 1 cut(s) 139
BspT107I GGYRCC 2 cut(s) 214, 842
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 2 cut(s) 15, 765
BssECI CCNNGG 2 cut(s) 141, 184
BssHII GCGCGC 1 cut(s) 307
BssMI GATC 2 cut(s) 223, 681
BssNI GRCGYC 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 862
Bst4CI ACNGT 4 cut(s) 219, 233, 536, 743
Bst6I CTCTTC 4 cut(s) 89, 139, 413, 630
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 5 cut(s) 107, 154, 351, 498, 804
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 2 cut(s) 406, 514
BstFNI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BstHHI GCGC 4 cut(s) 201, 309, 311, 524
BstKTI GATC 2 cut(s) 226, 684
BstMBI GATC 2 cut(s) 223, 681
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 5 cut(s) 28, 63, 212, 308, 401
BstNI CCWGG 1 cut(s) 862
BstSCI CCNGG 3 cut(s) 36, 141, 860
BstUI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
BstV1I GCAGC 3 cut(s) 44, 58, 252
BstV2I GAAGAC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 406, 514
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 4 cut(s) 201, 309, 311, 524
Cfr13I GGNCC 4 cut(s) 328, 385, 493, 763
Cfr42I CCGCGG 1 cut(s) 187
CseI GACGC 7 cut(s) 55, 157, 212, 308, 324, 401, 701
CsiI ACCWGGT 1 cut(s) 860
Csp6I GTAC 1 cut(s) 773
CviJI RGCY 8 cut(s) 5, 158, 279, 323, 355, 377, 611, 782
CviKI_1 RGCY 8 cut(s) 5, 158, 279, 323, 355, 377, 611, 782
CviQI GTAC 1 cut(s) 773
DdeI CTNAG 5 cut(s) 107, 154, 351, 498, 804
DpnI GATC 2 cut(s) 225, 683
DpnII GATC 2 cut(s) 223, 681
DriI GACNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 4 cut(s) 89, 139, 413, 630
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 4 cut(s) 89, 139, 413, 630
Eco47I GGWCC 4 cut(s) 328, 385, 493, 763
Eco57I CTGAAG 1 cut(s) 633
EcoRII CCWGG 1 cut(s) 860
FaiI YATR 8 cut(s) 174, 271, 273, 326, 410, 468, 562, 680
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 681
Fnu4HI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
FokI GGATG 2 cut(s) 413, 521
Fsp4HI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
FspBI CTAG 1 cut(s) 179
GlaI GCGC 4 cut(s) 200, 308, 310, 523
GluI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
HapII CCGG 4 cut(s) 38, 143, 212, 766
HgaI GACGC 7 cut(s) 55, 157, 212, 308, 324, 401, 701
HhaI GCGC 4 cut(s) 201, 309, 311, 524
Hin1I GRCGYC 1 cut(s) 168
Hin6I GCGC 4 cut(s) 199, 307, 309, 522
HinP1I GCGC 4 cut(s) 199, 307, 309, 522
HinfI GANTC 5 cut(s) 189, 251, 257, 517, 617
HpaII CCGG 4 cut(s) 38, 143, 212, 766
HphI GGTGA 2 cut(s) 694, 850
Hpy188I TCNGA 8 cut(s) 194, 223, 250, 256, 371, 571, 622, 805
Hpy188III TCNNGA 4 cut(s) 227, 383, 746, 766
Hpy99I CGWCG 4 cut(s) 206, 233, 265, 302
HpyAV CCTTC 3 cut(s) 349, 437, 447
HpyCH4III ACNGT 4 cut(s) 219, 233, 536, 743
HpyCH4V TGCA 3 cut(s) 46, 473, 553
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 63, 212, 308, 401
HpyF3I CTNAG 5 cut(s) 107, 154, 351, 498, 804
Hsp92I GRCGYC 1 cut(s) 168
HspAI GCGC 4 cut(s) 199, 307, 309, 522
Kpn2I TCCGGA 1 cut(s) 765
Ksp22I TGATCA 1 cut(s) 681
KspI CCGCGG 1 cut(s) 187
Kzo9I GATC 2 cut(s) 223, 681
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 2 cut(s) 65, 211
Lsp1109I GCAGC 3 cut(s) 44, 58, 252
LweI GCATC 3 cut(s) 391, 478, 499
MabI ACCWGGT 1 cut(s) 860
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 3 cut(s) 67, 233, 536
MalI GATC 2 cut(s) 225, 683
MbiI CCGCTC 1 cut(s) 132
MboI GATC 2 cut(s) 223, 681
MluCI AATT 6 cut(s) 121, 359, 463, 476, 749, 799
MlyI GAGTC 2 cut(s) 183, 266
MmeI TCCRAC 1 cut(s) 217
MroI TCCGGA 1 cut(s) 765
MseI TTAA 1 cut(s) 868
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 2 cut(s) 186, 243
MspI CCGG 4 cut(s) 38, 143, 212, 766
MspR9I CCNGG 3 cut(s) 38, 143, 862
MvaI CCWGG 1 cut(s) 862
MvnI CGCG 8 cut(s) 165, 186, 199, 201, 297, 309, 335, 522
MwoI GCNNNNNNNGC 5 cut(s) 28, 63, 212, 308, 401
NciI CCSGG 2 cut(s) 38, 143
NdeII GATC 2 cut(s) 223, 681
NlaIV GGNNCC 3 cut(s) 216, 495, 844
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 2 cut(s) 67, 536
PalAI GGCGCGCC 1 cut(s) 307
PauI GCGCGC 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PfeI GAWTC 3 cut(s) 251, 517, 617
PkrI GCNGC 6 cut(s) 48, 59, 131, 167, 242, 281
PleI GAGTC 2 cut(s) 183, 265
PpsI GAGTC 2 cut(s) 183, 265
Psp6I CCWGG 1 cut(s) 860
PspGI CCWGG 1 cut(s) 860
PspN4I GGNNCC 3 cut(s) 216, 495, 844
PspPI GGNCC 4 cut(s) 328, 385, 493, 763
PteI GCGCGC 1 cut(s) 307
RsaI GTAC 1 cut(s) 774
RsaNI GTAC 1 cut(s) 773
RseI CAYNNNNRTG 1 cut(s) 651
SacII CCGCGG 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 139
SaqAI TTAA 1 cut(s) 868
SatI GCNGC 6 cut(s) 47, 58, 130, 166, 241, 280
Sau3AI GATC 2 cut(s) 223, 681
Sau96I GGNCC 4 cut(s) 328, 385, 493, 763
SchI GAGTC 2 cut(s) 183, 266
ScrFI CCNGG 3 cut(s) 38, 143, 862
SetI ASST 9 cut(s) 98, 160, 333, 352, 357, 576, 613, 784, 866
SexAI ACCWGGT 1 cut(s) 860
SfaNI GCATC 3 cut(s) 391, 478, 499
Sfr303I CCGCGG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 187
SgsI GGCGCGCC 1 cut(s) 307
SinI GGWCC 4 cut(s) 328, 385, 493, 763
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 793
SmoI CTYRAG 1 cut(s) 793
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 6 cut(s) 121, 359, 463, 476, 749, 799
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 3 cut(s) 36, 141, 860
TaaI ACNGT 4 cut(s) 219, 233, 536, 743
TaqI TCGA 2 cut(s) 228, 263
TasI AATT 6 cut(s) 121, 359, 463, 476, 749, 799
TatI WGTACW 1 cut(s) 772
TauI GCSGC 3 cut(s) 132, 168, 282
TfiI GAWTC 3 cut(s) 251, 517, 617
Tru1I TTAA 1 cut(s) 868
Tru9I TTAA 1 cut(s) 868
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 2 cut(s) 67, 536
TseI GCWGC 3 cut(s) 46, 57, 240
Tsp45I GTSAC 2 cut(s) 67, 536
TspDTI ATGAA 2 cut(s) 723, 843
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 4 cut(s) 328, 385, 493, 763
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.