FvH4_3g00660

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011137
Physical Location & Seq
Forward (+)
314297 .. 316490
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00660.t1

Sequence Viewer

Length: 333 bp
ATGAACACGGACATCACAGCGTCGACGAAGCCCGAGTACCCGGTCTTAGATCGGAACCCTCCCTTCACCAGAGTCGTCGGCAACTTCAGCACCCTCGACTATTTCCGTTTCGCCACCATCACCGGCGTCTCCGTCACCGTCGGCTACCTCTCCGGGATTAAGCCCGGGATTAGAGGGCCGTCAATGGTGACCGGAGGTCTGATTGGAGTGATGGGCGGGTTCATGTACGCTTACCAGAACTCGGCGGGTCGCATCATGGGCTTTTTCCCCAACGACCATGAGTCGCATGTGTTTGCAGATGAACAAATTGTTTTTGTAACCAACACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

111

Amino Acids

11.95

Weight (kDa)

6.02

Isoelectric Point (pI)

14.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADH-u_ox-rdase PF10785 11 - 89 6.8e-19 NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 23
AciI CCGC 2 cut(s) 216, 245
AcuI CTGAAG 1 cut(s) 70
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 2 cut(s) 38, 227
AfiI CCNNNNNNNGG 1 cut(s) 241
AhdI GACNNNNNGTC 2 cut(s) 195, 280
Alw26I GTCTC 1 cut(s) 133
Ama87I CYCGRG 2 cut(s) 32, 164
AoxI GGCC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 4 cut(s) 41, 154, 165, 166
AsuHPI GGTGA 4 cut(s) 58, 112, 127, 199
AvaI CYCGRG 2 cut(s) 32, 164
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BccI CCATC 2 cut(s) 125, 205
BceAI ACGGC 1 cut(s) 163
BcnI CCSGG 4 cut(s) 41, 154, 165, 166
BcoDI GTCTC 1 cut(s) 133
Bme1390I CCNGG 4 cut(s) 41, 154, 165, 166
BmeRI GACNNNNNGTC 2 cut(s) 195, 280
BmeT110I CYCGRG 2 cut(s) 32, 164
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 4 cut(s) 41, 154, 165, 166
BmsI GCATC 1 cut(s) 261
BpuMI CCSGG 4 cut(s) 41, 154, 165, 166
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 164
BsaWI WCCGGW 1 cut(s) 191
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse118I RCCGGY 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 241
BshFI GGCC 1 cut(s) 178
BsiHKCI CYCGRG 2 cut(s) 32, 164
BsiSI CCGG 5 cut(s) 41, 123, 153, 165, 192
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsnI GGCC 1 cut(s) 178
BsoBI CYCGRG 2 cut(s) 32, 164
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 2 cut(s) 216, 245
BspANI GGCC 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 139
BstACI GRCGYC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 46
BstEII GGTNACC 1 cut(s) 187
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 2 cut(s) 87, 258
BstNSI RCATGY 1 cut(s) 290
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 4 cut(s) 39, 152, 163, 164
BsuRI GGCC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 176
Cfr9I CCCGGG 1 cut(s) 164
CseI GACGC 2 cut(s) 9, 115
Csp6I GTAC 2 cut(s) 37, 226
CviAII CATG 4 cut(s) 223, 256, 278, 287
CviJI RGCY 5 cut(s) 31, 144, 163, 178, 261
CviKI_1 RGCY 5 cut(s) 31, 144, 163, 178, 261
CviQI GTAC 2 cut(s) 37, 226
DdeI CTNAG 1 cut(s) 46
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DriI GACNNNNNGTC 2 cut(s) 195, 280
Eam1105I GACNNNNNGTC 2 cut(s) 195, 280
Eco57I CTGAAG 1 cut(s) 70
Eco88I CYCGRG 2 cut(s) 32, 164
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 4 cut(s) 226, 259, 281, 290
FaiI YATR 4 cut(s) 224, 257, 279, 288
FatI CATG 4 cut(s) 222, 255, 277, 286
FauI CCCGC 2 cut(s) 209, 238
FblI GTMKAC 1 cut(s) 23
HaeIII GGCC 1 cut(s) 178
HapII CCGG 5 cut(s) 41, 123, 153, 165, 192
HgaI GACGC 2 cut(s) 9, 115
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 4 cut(s) 226, 259, 281, 290
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 2 cut(s) 72, 281
HpaII CCGG 5 cut(s) 41, 123, 153, 165, 192
HphI GGTGA 4 cut(s) 58, 112, 127, 199
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 2 cut(s) 54, 201
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 4 cut(s) 25, 28, 80, 143
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 258
HpyF3I CTNAG 1 cut(s) 46
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 4 cut(s) 226, 259, 281, 290
Kzo9I GATC 1 cut(s) 49
LpnPI CCDG 7 cut(s) 54, 82, 136, 166, 178, 205, 248
LweI GCATC 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 133, 187, 316
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MluCI AATT 1 cut(s) 306
MlyI GAGTC 2 cut(s) 81, 290
MnlI CCTC 5 cut(s) 69, 104, 158, 167, 188
MseI TTAA 1 cut(s) 159
MspI CCGG 5 cut(s) 41, 123, 153, 165, 192
MspR9I CCNGG 4 cut(s) 41, 154, 165, 166
MwoI GCNNNNNNNGC 2 cut(s) 87, 258
NciI CCSGG 4 cut(s) 41, 154, 165, 166
NdeII GATC 1 cut(s) 49
NlaIII CATG 4 cut(s) 226, 259, 281, 290
NlaIV GGNNCC 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 133, 187
NspI RCATGY 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 152
PleI GAGTC 2 cut(s) 80, 289
PpsI GAGTC 2 cut(s) 80, 289
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 176
RsaI GTAC 2 cut(s) 38, 227
RsaNI GTAC 2 cut(s) 37, 226
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 2 cut(s) 81, 290
ScrFI CCNGG 4 cut(s) 41, 154, 165, 166
SetI ASST 2 cut(s) 150, 199
SfaNI GCATC 1 cut(s) 261
SgrAI CRCCGGYG 1 cut(s) 122
SgrDI CGTCGACG 1 cut(s) 22
SmaI CCCGGG 1 cut(s) 166
Sse9I AATT 1 cut(s) 306
SsiI CCGC 2 cut(s) 216, 245
StyD4I CCNGG 4 cut(s) 39, 152, 163, 164
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 2 cut(s) 23, 96
TasI AATT 1 cut(s) 306
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseFI GTSAC 2 cut(s) 133, 187
Tsp45I GTSAC 2 cut(s) 133, 187
TspDTI ATGAA 3 cut(s) 17, 211, 315
TspGWI ACGGA 3 cut(s) 23, 95, 121
TspMI CCCGGG 1 cut(s) 164
XceI RCATGY 1 cut(s) 290
XmaI CCCGGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.