MD10G1336200.v1.1

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011137
Physical Location & Seq
Reverse (-)
41275259 .. 41276509
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1336200.v1.1.491

Sequence Viewer

Length: 303 bp
ATGAATACAGACATCACAGCGTCAACGAAGCCCGAGTACCCGGTCATAGATCGGAACCCTCCGTTCACCACAGTCGTCGGCAACTTCAGCACCCTCGATTACTTCCGTTTCGCCACCATCACCGGCGTCTCCGTTACCGTCGGCTACCTCTCCGGGATTAAGCCCGGGATTAGAGGGCCGTCAATGGTTACAGGAGGTTTGATTGGGGTAATGGGTGGATTCATGTACGCTTACCAGAACTCGGCGGGACGCCTCATGGCCTTTTTCCCCAACGACGATGAGGTAGCTCGTCACAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

101

Amino Acids

10.83

Weight (kDa)

9.1

Isoelectric Point (pI)

21.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADH-u_ox-rdase PF10785 11 - 88 2.2e-18 NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 70
AcyI GRCGYC 2 cut(s) 126, 250
AfaI GTAC 2 cut(s) 38, 227
AfiI CCNNNNNNNGG 1 cut(s) 241
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Alw26I GTCTC 1 cut(s) 133
Ama87I CYCGRG 2 cut(s) 32, 164
AoxI GGCC 2 cut(s) 176, 258
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 4 cut(s) 41, 154, 165, 166
AsuHPI GGTGA 2 cut(s) 58, 112
AvaI CYCGRG 2 cut(s) 32, 164
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 163
BcnI CCSGG 4 cut(s) 41, 154, 165, 166
BcoDI GTCTC 1 cut(s) 133
Bme1390I CCNGG 4 cut(s) 41, 154, 165, 166
BmeT110I CYCGRG 2 cut(s) 32, 164
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 4 cut(s) 41, 154, 165, 166
BpuMI CCSGG 4 cut(s) 41, 154, 165, 166
BsaHI GRCGYC 2 cut(s) 126, 250
BsaJI CCNNGG 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse118I RCCGGY 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 241
BshFI GGCC 2 cut(s) 178, 260
BsiHKCI CYCGRG 2 cut(s) 32, 164
BsiSI CCGG 4 cut(s) 41, 123, 153, 165
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 2 cut(s) 178, 260
BsoBI CYCGRG 2 cut(s) 32, 164
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 1 cut(s) 245
BspANI GGCC 2 cut(s) 178, 260
BspLI GGNNCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 2 cut(s) 126, 250
Bst4CI ACNGT 2 cut(s) 73, 139
BstACI GRCGYC 2 cut(s) 126, 250
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 87
BstSCI CCNGG 4 cut(s) 39, 152, 163, 164
BsuRI GGCC 2 cut(s) 178, 260
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 176
Cfr9I CCCGGG 1 cut(s) 164
CseI GACGC 3 cut(s) 9, 115, 258
Csp6I GTAC 2 cut(s) 37, 226
CviAII CATG 2 cut(s) 223, 256
CviJI RGCY 6 cut(s) 31, 144, 163, 178, 260, 287
CviKI_1 RGCY 6 cut(s) 31, 144, 163, 178, 260, 287
CviQI GTAC 2 cut(s) 37, 226
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 70
Eco88I CYCGRG 2 cut(s) 32, 164
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 2 cut(s) 226, 259
FaiI YATR 3 cut(s) 47, 224, 257
FaqI GGGAC 1 cut(s) 261
FatI CATG 2 cut(s) 222, 255
FauI CCCGC 1 cut(s) 238
HaeIII GGCC 2 cut(s) 178, 260
HapII CCGG 4 cut(s) 41, 123, 153, 165
HgaI GACGC 3 cut(s) 9, 115, 258
Hin1I GRCGYC 2 cut(s) 126, 250
Hin1II CATG 2 cut(s) 226, 259
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 1 cut(s) 219
HpaII CCGG 4 cut(s) 41, 123, 153, 165
HphI GGTGA 2 cut(s) 58, 112
Hpy166II GTNNAC 2 cut(s) 24, 66
Hpy188I TCNGA 1 cut(s) 54
Hpy8I GTNNAC 2 cut(s) 24, 66
Hpy99I CGWCG 3 cut(s) 80, 143, 278
HpyCH4III ACNGT 2 cut(s) 73, 139
HpyF10VI GCNNNNNNNGC 1 cut(s) 87
Hsp92I GRCGYC 2 cut(s) 126, 250
Hsp92II CATG 2 cut(s) 226, 259
Kzo9I GATC 1 cut(s) 49
LpnPI CCDG 6 cut(s) 54, 136, 166, 177, 178, 248
MaeIII GTNAC 3 cut(s) 133, 187, 290
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MnlI CCTC 7 cut(s) 69, 104, 158, 167, 188, 263, 274
MseI TTAA 1 cut(s) 159
MspI CCGG 4 cut(s) 41, 123, 153, 165
MspR9I CCNGG 4 cut(s) 41, 154, 165, 166
MwoI GCNNNNNNNGC 1 cut(s) 87
NciI CCSGG 4 cut(s) 41, 154, 165, 166
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 226, 259
NlaIV GGNNCC 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 219
PfoI TCCNGGA 1 cut(s) 152
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 176
RsaI GTAC 2 cut(s) 38, 227
RsaNI GTAC 2 cut(s) 37, 226
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 4 cut(s) 41, 154, 165, 166
SetI ASST 4 cut(s) 150, 199, 285, 289
SgrAI CRCCGGYG 1 cut(s) 122
SmaI CCCGGG 1 cut(s) 166
SsiI CCGC 1 cut(s) 245
StyD4I CCNGG 4 cut(s) 39, 152, 163, 164
TaaI ACNGT 2 cut(s) 73, 139
TaqI TCGA 1 cut(s) 96
TfiI GAWTC 1 cut(s) 219
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseFI GTSAC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 2 cut(s) 17, 211
TspGWI ACGGA 3 cut(s) 51, 95, 121
TspMI CCCGGG 1 cut(s) 164
XmaI CCCGGG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.