Rorug04G0387300

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011137
Physical Location & Seq
Reverse (-)
55635856 .. 55638218
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0387300.1

Sequence Viewer

Length: 627 bp
ATGGATCTACAGCAGCAGAACGCAAGAGCTCCACAGCCTCGCCGCGGCGGCGGTTTCTCGGCCCAGTCGTCTTTCCTCTTCCATCTAATGGTCATTTTGTTTGTGCTAGAAGCCTTTCTGCACCAAGTCCCGGAAGGTCATGTTGGGGTTTATTGGAGAGGAGATACTCTTCTTAAGACTGTTACATATCCGGGGGTTCATCTCAAGTTGCCTATGATATACTTTGAACCTATTCAAGTGACCCTTCAGACTCATCAGTTTTGTGATATTCCATGCGGTACAAAAGATGATGTCATGATTAACATTGAGAAGATAGAGGTTGTTAACCGGCTTCGCAAAGAATACGTGTATGAGACATTAAGGAGTTATGGGACTCAGTATTACTGCTCATATACGTGTGTCGTCGTGCGTAGAGAGATCAGTAAGCTCTGCGGCTCACATTCTATTGAAGAGGTTTTTGATCAGATTGATGAAAAGCTGAGAGAAGCTCTTCACGATGATAGTGCGCATTATGCTCCCGGCATTGAAATCATCAGCGTGCGTGTCACAAGGCCTACTATCCCAGATAGTGTAAGACGCGGAACACACCAAGGTACCAAAACTGTATGGGAGACATGTGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

208

Amino Acids

23.93

Weight (kDa)

6.3

Isoelectric Point (pI)

48.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 42 - 194 2e-16 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 507
Acc65I GGTACC 1 cut(s) 593
AccB1I GGYRCC 1 cut(s) 593
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 2 cut(s) 45, 579
AciI CCGC 7 cut(s) 43, 45, 48, 51, 276, 432, 579
AclWI GGATC 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 230
AfaI GTAC 2 cut(s) 280, 595
AfiI CCNNNNNNNGG 3 cut(s) 44, 88, 130
AflII CTTAAG 1 cut(s) 173
AflIII ACRYGT 3 cut(s) 345, 395, 614
AgsI TTSAA 4 cut(s) 227, 236, 449, 527
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 4 cut(s) 29, 427, 478, 488
AluI AGCT 4 cut(s) 29, 427, 478, 488
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 2 cut(s) 347, 605
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 60, 551
ApeKI GCWGC 1 cut(s) 13
Asp700I GAANNNNTTC 3 cut(s) 114, 231, 489
Asp718I GGTACC 1 cut(s) 593
AspLEI GCGC 1 cut(s) 508
AspS9I GGNCC 1 cut(s) 61
AsuC2I CCSGG 3 cut(s) 131, 192, 519
BaeI ACNNNNGTAYC 2 cut(s) 577, 610
BanI GGYRCC 1 cut(s) 593
BanII GRGCYC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 25
BccI CCATC 1 cut(s) 90
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 3 cut(s) 131, 192, 519
BcoDI GTCTC 2 cut(s) 347, 605
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 173
BglI GCCNNNNNGGC 1 cut(s) 48
BisI GCNGC 5 cut(s) 14, 43, 46, 49, 433
BlsI GCNGC 5 cut(s) 15, 44, 47, 50, 434
Bme1390I CCNGG 3 cut(s) 131, 192, 519
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 595
BmrFI CCNGG 3 cut(s) 131, 192, 519
BmrI ACTGGG 1 cut(s) 58
BmuI ACTGGG 1 cut(s) 58
BplI GAGNNNNNCTC 2 cut(s) 472, 504
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 3 cut(s) 131, 192, 519
BsaAI YACGTR 2 cut(s) 346, 396
BsaJI CCNNGG 3 cut(s) 43, 191, 589
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 88, 130
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 64
BseDI CCNNGG 3 cut(s) 43, 191, 589
BseLI CCNNNNNNNGG 3 cut(s) 44, 88, 130
BseMII CTCAG 2 cut(s) 389, 470
BseNI ACTGG 1 cut(s) 64
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 104
Bsh1236I CGCG 2 cut(s) 45, 579
BshFI GGCC 2 cut(s) 62, 553
BshNI GGYRCC 1 cut(s) 593
BsiHKAI GWGCWC 1 cut(s) 31
BsiSI CCGG 4 cut(s) 131, 191, 328, 519
BslFI GGGAC 2 cut(s) 113, 385
BslI CCNNNNNNNGG 3 cut(s) 44, 88, 130
BsmAI GTCTC 2 cut(s) 347, 605
BsmFI GGGAC 2 cut(s) 113, 385
BsnI GGCC 2 cut(s) 62, 553
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 3 cut(s) 4, 417, 460
BspACI CCGC 7 cut(s) 43, 45, 48, 51, 276, 432, 579
BspANI GGCC 2 cut(s) 62, 553
BspCNI CTCAG 2 cut(s) 388, 471
BspFNI CGCG 2 cut(s) 45, 579
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 595
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 495
BspT107I GGYRCC 1 cut(s) 593
BspTI CTTAAG 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 3 cut(s) 43, 191, 589
BssMI GATC 3 cut(s) 4, 417, 460
BssT1I CCWWGG 1 cut(s) 589
Bst4CI ACNGT 2 cut(s) 181, 604
Bst6I CTCTTC 4 cut(s) 83, 174, 444, 495
BstAFI CTTAAG 1 cut(s) 173
BstBAI YACGTR 2 cut(s) 346, 396
BstC8I GCNNGC 1 cut(s) 539
BstDEI CTNAG 2 cut(s) 375, 479
BstDSI CCRYGG 1 cut(s) 43
BstFNI CGCG 2 cut(s) 45, 579
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 3 cut(s) 7, 420, 463
BstMAI GTCTC 2 cut(s) 347, 605
BstMBI GATC 3 cut(s) 4, 417, 460
BstMWI GCNNNNNNNGC 2 cut(s) 48, 512
BstNSI RCATGY 1 cut(s) 618
BstSCI CCNGG 3 cut(s) 129, 190, 517
BstSFI CTRYAG 1 cut(s) 8
BstUI CGCG 2 cut(s) 45, 579
BstV1I GCAGC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 62, 553
BtgI CCRYGG 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 539
CciI TCATGA 1 cut(s) 294
CfoI GCGC 1 cut(s) 508
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 61
Cfr42I CCGCGG 1 cut(s) 46
CseI GACGC 1 cut(s) 585
Csp6I GTAC 2 cut(s) 279, 594
CviAII CATG 4 cut(s) 140, 273, 295, 615
CviQI GTAC 2 cut(s) 279, 594
DdeI CTNAG 2 cut(s) 375, 479
DpnI GATC 3 cut(s) 6, 419, 462
DpnII GATC 3 cut(s) 4, 417, 460
Eam1104I CTCTTC 4 cut(s) 83, 174, 444, 495
EarI CTCTTC 4 cut(s) 83, 174, 444, 495
Ecl136II GAGCTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 589
Eco147I AGGCCT 1 cut(s) 553
Eco24I GRGCYC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 29
Eco57I CTGAAG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 589
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 589
FaeI CATG 4 cut(s) 143, 276, 298, 618
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 2 cut(s) 113, 385
FatI CATG 4 cut(s) 139, 272, 294, 614
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 5 cut(s) 14, 43, 46, 49, 433
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 5 cut(s) 14, 43, 46, 49, 433
FspAI RTGCGCAY 1 cut(s) 507
FspBI CTAG 1 cut(s) 107
FspI TGCGCA 1 cut(s) 507
GlaI GCGC 1 cut(s) 507
GluI GCNGC 5 cut(s) 14, 43, 46, 49, 433
HaeIII GGCC 2 cut(s) 62, 553
HapII CCGG 4 cut(s) 131, 191, 328, 519
HgaI GACGC 1 cut(s) 585
HhaI GCGC 1 cut(s) 508
Hin1II CATG 4 cut(s) 143, 276, 298, 618
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 2 cut(s) 250, 373
HpaI GTTAAC 1 cut(s) 325
HpaII CCGG 4 cut(s) 131, 191, 328, 519
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 2 cut(s) 249, 465
Hpy188III TCNNGA 2 cut(s) 295, 494
Hpy8I GTNNAC 1 cut(s) 325
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 2 cut(s) 128, 254
HpyCH4III ACNGT 2 cut(s) 181, 604
HpyCH4IV ACGT 2 cut(s) 345, 395
HpyCH4V TGCA 1 cut(s) 121
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 512
HpyF3I CTNAG 2 cut(s) 375, 479
HpySE526I ACGT 2 cut(s) 345, 395
Hsp92II CATG 4 cut(s) 143, 276, 298, 618
HspAI GCGC 1 cut(s) 506
KpnI GGTACC 1 cut(s) 597
Ksp22I TGATCA 1 cut(s) 460
KspAI GTTAAC 1 cut(s) 325
KspI CCGCGG 1 cut(s) 46
Kzo9I GATC 3 cut(s) 4, 417, 460
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 34, 520
LpnPI CCDG 6 cut(s) 77, 144, 204, 341, 532, 576
Lsp1109I GCAGC 1 cut(s) 25
MaeI CTAG 1 cut(s) 107
MaeII ACGT 2 cut(s) 345, 395
MaeIII GTNAC 3 cut(s) 181, 238, 544
MalI GATC 3 cut(s) 6, 419, 462
MboI GATC 3 cut(s) 4, 417, 460
MboII GAAGA 5 cut(s) 70, 161, 322, 461, 482
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 31
MlyI GAGTC 2 cut(s) 244, 367
MnlI CCTC 5 cut(s) 48, 86, 152, 310, 445
MroXI GAANNNNTTC 3 cut(s) 114, 231, 489
MseI TTAA 4 cut(s) 174, 300, 324, 359
MslI CAYNNNNRTG 2 cut(s) 394, 536
MspA1I CMGCKG 1 cut(s) 45
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 4 cut(s) 131, 191, 328, 519
MspR9I CCNGG 3 cut(s) 131, 192, 519
MvnI CGCG 2 cut(s) 45, 579
MwoI GCNNNNNNNGC 2 cut(s) 48, 512
NciI CCSGG 3 cut(s) 131, 192, 519
NdeII GATC 3 cut(s) 4, 417, 460
NlaIII CATG 4 cut(s) 143, 276, 298, 618
NlaIV GGNNCC 1 cut(s) 595
NmeAIII GCCGAG 1 cut(s) 38
NmuCI GTSAC 2 cut(s) 238, 544
NsbI TGCGCA 1 cut(s) 507
NspI RCATGY 1 cut(s) 618
PagI TCATGA 1 cut(s) 294
PceI AGGCCT 1 cut(s) 553
PciI ACATGT 1 cut(s) 614
PciSI GCTCTTC 1 cut(s) 495
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PdmI GAANNNNTTC 3 cut(s) 114, 231, 489
PflMI CCANNNNNTGG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 5 cut(s) 15, 44, 47, 50, 434
PleI GAGTC 2 cut(s) 244, 367
PpsI GAGTC 2 cut(s) 244, 367
Ppu21I YACGTR 2 cut(s) 346, 396
PscI ACATGT 1 cut(s) 614
Psp124BI GAGCTC 1 cut(s) 31
PspN4I GGNNCC 1 cut(s) 595
PspPI GGNCC 1 cut(s) 61
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 280, 595
RsaNI GTAC 2 cut(s) 279, 594
RseI CAYNNNNRTG 2 cut(s) 394, 536
SacI GAGCTC 1 cut(s) 31
SacII CCGCGG 1 cut(s) 46
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 4 cut(s) 174, 300, 324, 359
SatI GCNGC 5 cut(s) 14, 43, 46, 49, 433
Sau3AI GATC 3 cut(s) 4, 417, 460
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 2 cut(s) 244, 367
ScrFI CCNGG 3 cut(s) 131, 192, 519
SduI GDGCHC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 8
Sfr303I CCGCGG 1 cut(s) 46
SgrBI CCGCGG 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 394, 536
SmlI CTYRAG 2 cut(s) 173, 203
SmoI CTYRAG 2 cut(s) 173, 203
SseBI AGGCCT 1 cut(s) 553
SsiI CCGC 7 cut(s) 43, 45, 48, 51, 276, 432, 579
SspMI CTAG 1 cut(s) 107
SstI GAGCTC 1 cut(s) 31
StuI AGGCCT 1 cut(s) 553
StyD4I CCNGG 3 cut(s) 129, 190, 517
StyI CCWWGG 1 cut(s) 589
TaaI ACNGT 2 cut(s) 181, 604
TaiI ACGT 2 cut(s) 348, 398
TauI GCSGC 4 cut(s) 45, 48, 51, 435
Tru1I TTAA 4 cut(s) 174, 300, 324, 359
Tru9I TTAA 4 cut(s) 174, 300, 324, 359
TseFI GTSAC 2 cut(s) 238, 544
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 2 cut(s) 238, 544
TspDTI ATGAA 2 cut(s) 188, 486
Van91I CCANNNNNTGG 1 cut(s) 88
Vha464I CTTAAG 1 cut(s) 173
XceI RCATGY 1 cut(s) 618
XmnI GAANNNNTTC 3 cut(s) 114, 231, 489
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.