Rw5G000740

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011137
Physical Location & Seq
Forward (+)
512997 .. 513384
388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000740.1

Sequence Viewer

Length: 303 bp
ATGAACACAGACATCACAGCGTCGACGAAGCCCGAGTACCCGGTCTTAGATCGGAACCCTCCCTTCACCAGAGTCGTCGGCAATTTCAGCACCCTCGACTATTTCCGATTCGCCACCATCACCGGCGTGTCCGTCACCGTCGGCTACCTCTCCGGAATTAAGCCGGGAATTAGAGGGCCGTCTATGGTGACCGGAGGTCTGATTGGAGTGATGGGCGGGTTCATGTACGCTTACCAGAACTCGGCGGGCCGCATCATGGGCTTTTTCCCCAACGACCGTGAGGTAGCCCATTACAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

100

Amino Acids

10.92

Weight (kDa)

9.63

Isoelectric Point (pI)

18.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADH-u_ox-rdase PF10785 11 - 89 5e-19 NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 3 cut(s) 216, 245, 250
AfaI GTAC 2 cut(s) 38, 227
AfiI CCNNNNNNNGG 2 cut(s) 241, 256
AhdI GACNNNNNGTC 1 cut(s) 195
AleI CACNNNNGTG 1 cut(s) 125
Ama87I CYCGRG 1 cut(s) 32
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 2 cut(s) 176, 247
AspS9I GGNCC 2 cut(s) 176, 247
AsuC2I CCSGG 2 cut(s) 41, 165
AsuHPI GGTGA 4 cut(s) 58, 112, 127, 199
AvaI CYCGRG 1 cut(s) 32
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BccI CCATC 2 cut(s) 125, 205
BceAI ACGGC 1 cut(s) 163
BcnI CCSGG 2 cut(s) 41, 165
BisI GCNGC 1 cut(s) 250
BlsI GCNGC 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 41, 165
BmeRI GACNNNNNGTC 1 cut(s) 195
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 2 cut(s) 176, 247
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 2 cut(s) 41, 165
BmsI GCATC 1 cut(s) 261
BpuMI CCSGG 2 cut(s) 41, 165
BsaWI WCCGGW 2 cut(s) 152, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 256
Bse118I RCCGGY 1 cut(s) 122
BseAI TCCGGA 1 cut(s) 152
BseLI CCNNNNNNNGG 2 cut(s) 241, 256
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 2 cut(s) 178, 249
BsiEI CGRYCG 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 5 cut(s) 41, 123, 153, 164, 192
BslI CCNNNNNNNGG 2 cut(s) 241, 256
BsnI GGCC 2 cut(s) 178, 249
BsoBI CYCGRG 1 cut(s) 32
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 3 cut(s) 216, 245, 250
BspANI GGCC 2 cut(s) 178, 249
BspEI TCCGGA 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssMI GATC 1 cut(s) 49
Bst4CI ACNGT 2 cut(s) 139, 278
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 1 cut(s) 46
BstEII GGTNACC 1 cut(s) 187
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 87, 258
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 2 cut(s) 39, 163
BsuRI GGCC 2 cut(s) 178, 249
Cac8I GCNNGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 176, 247
CseI GACGC 1 cut(s) 9
Csp6I GTAC 2 cut(s) 37, 226
CviAII CATG 2 cut(s) 223, 256
CviJI RGCY 7 cut(s) 31, 144, 163, 178, 249, 261, 287
CviKI_1 RGCY 7 cut(s) 31, 144, 163, 178, 249, 261, 287
CviQI GTAC 2 cut(s) 37, 226
DdeI CTNAG 1 cut(s) 46
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
FaeI CATG 2 cut(s) 226, 259
FaiI YATR 3 cut(s) 185, 224, 257
FatI CATG 2 cut(s) 222, 255
FauI CCCGC 2 cut(s) 209, 238
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 1 cut(s) 250
Fsp4HI GCNGC 1 cut(s) 250
GluI GCNGC 1 cut(s) 250
HaeIII GGCC 2 cut(s) 178, 249
HapII CCGG 5 cut(s) 41, 123, 153, 164, 192
HgaI GACGC 1 cut(s) 9
Hin1II CATG 2 cut(s) 226, 259
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 2 cut(s) 72, 108
HpaII CCGG 5 cut(s) 41, 123, 153, 164, 192
HphI GGTGA 4 cut(s) 58, 112, 127, 199
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 54, 107, 201
Hpy188III TCNNGA 1 cut(s) 153
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 4 cut(s) 25, 28, 80, 143
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 2 cut(s) 139, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 258
HpyF3I CTNAG 1 cut(s) 46
Hsp92II CATG 2 cut(s) 226, 259
Kpn2I TCCGGA 1 cut(s) 152
Kzo9I GATC 1 cut(s) 49
LpnPI CCDG 7 cut(s) 54, 82, 136, 166, 177, 205, 248
LweI GCATC 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 133, 187
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MluCI AATT 3 cut(s) 82, 156, 168
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 6 cut(s) 69, 104, 158, 167, 188, 274
MroI TCCGGA 1 cut(s) 152
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 125
MspI CCGG 5 cut(s) 41, 123, 153, 164, 192
MspR9I CCNGG 2 cut(s) 41, 165
MwoI GCNNNNNNNGC 2 cut(s) 87, 258
NciI CCSGG 2 cut(s) 41, 165
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 226, 259
NlaIV GGNNCC 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 133, 187
OliI CACNNNNGTG 1 cut(s) 125
PfeI GAWTC 1 cut(s) 108
PkrI GCNGC 1 cut(s) 251
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 2 cut(s) 176, 247
RsaI GTAC 2 cut(s) 38, 227
RsaNI GTAC 2 cut(s) 37, 226
RseI CAYNNNNRTG 1 cut(s) 125
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 1 cut(s) 250
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 2 cut(s) 176, 247
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 2 cut(s) 41, 165
SetI ASST 3 cut(s) 150, 199, 285
SfaNI GCATC 1 cut(s) 261
SgrAI CRCCGGYG 1 cut(s) 122
SgrDI CGTCGACG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 3 cut(s) 82, 156, 168
SsiI CCGC 3 cut(s) 216, 245, 250
StyD4I CCNGG 2 cut(s) 39, 163
TaaI ACNGT 2 cut(s) 139, 278
TaqI TCGA 2 cut(s) 23, 96
TasI AATT 3 cut(s) 82, 156, 168
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseFI GTSAC 2 cut(s) 133, 187
Tsp45I GTSAC 2 cut(s) 133, 187
TspDTI ATGAA 2 cut(s) 17, 211
TspGWI ACGGA 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.