Rroxscaffold_1G00075370

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011137
Physical Location & Seq
Reverse (-)
96121383 .. 96122872
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075370.1

Sequence Viewer

Length: 387 bp
ATGAACACAGACATCACAGCGTCGACGAAGCCCGAGTACCCGGTCTTAGATCGGAACCCTCCCTTCACCAGAGTCGTCGGCAATTTCAGCACCCTCGACTATTTCCGATTCGCCACCATCACCGGCGTCTCCGTCACCGTCGGCTACCTCTCCGGAATTAAGCCGGGAATTAGAGGGCCGTCTATGGTGACCGGAGGTCTGATTGGAGTGATGGGCGGGTTCATGTACGCTTACCAGAACTCGGCGGGTCGCATCATGGGCTTTTTCCCCAACGACCGTGAGATGAAACTAAAGAAGGTGGATGAGGGAGTACATGACACGAGTACTCAAGAATATAGAGCCTGGAGTAAGGCAAAGATAGGTAAAGCAGTGAAGCAGGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

128

Amino Acids

13.99

Weight (kDa)

9.69

Isoelectric Point (pI)

9.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADH-u_ox-rdase PF10785 11 - 89 1.1e-18 NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 2 cut(s) 216, 245
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 4 cut(s) 38, 227, 312, 325
AfiI CCNNNNNNNGG 1 cut(s) 241
AhdI GACNNNNNGTC 1 cut(s) 195
AjnI CCWGG 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 32
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 2 cut(s) 41, 165
AsuHPI GGTGA 4 cut(s) 58, 112, 127, 199
AvaI CYCGRG 1 cut(s) 32
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BauI CACGAG 1 cut(s) 319
BccI CCATC 2 cut(s) 125, 205
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 1 cut(s) 343
BcnI CCSGG 2 cut(s) 41, 165
BcoDI GTCTC 1 cut(s) 133
BmcAI AGTACT 1 cut(s) 325
Bme1390I CCNGG 3 cut(s) 41, 165, 343
BmeRI GACNNNNNGTC 1 cut(s) 195
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 3 cut(s) 41, 165, 343
BmsI GCATC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 364
BpuEI CTTGAG 1 cut(s) 312
BpuMI CCSGG 2 cut(s) 41, 165
BsaHI GRCGYC 1 cut(s) 126
BsaWI WCCGGW 2 cut(s) 152, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse118I RCCGGY 1 cut(s) 122
BseAI TCCGGA 1 cut(s) 152
BseBI CCWGG 1 cut(s) 343
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 1 cut(s) 241
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 1 cut(s) 178
BsiEI CGRYCG 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 5 cut(s) 41, 123, 153, 164, 192
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsnI GGCC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 32
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 2 cut(s) 216, 245
BspANI GGCC 1 cut(s) 178
BspEI TCCGGA 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 319
Bst2BI CACGAG 1 cut(s) 319
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 2 cut(s) 139, 278
BstACI GRCGYC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 46
BstEII GGTNACC 1 cut(s) 187
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 49
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 87, 258
BstNI CCWGG 1 cut(s) 343
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 39, 163, 341
BsuRI GGCC 1 cut(s) 178
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 2 cut(s) 9, 115
Csp6I GTAC 4 cut(s) 37, 226, 311, 324
CviAII CATG 3 cut(s) 223, 256, 314
CviJI RGCY 6 cut(s) 31, 144, 163, 178, 261, 341
CviKI_1 RGCY 6 cut(s) 31, 144, 163, 178, 261, 341
CviQI GTAC 4 cut(s) 37, 226, 311, 324
DdeI CTNAG 1 cut(s) 46
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 341
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 3 cut(s) 226, 259, 317
FaiI YATR 5 cut(s) 185, 224, 257, 315, 336
FatI CATG 3 cut(s) 222, 255, 313
FauI CCCGC 2 cut(s) 209, 238
FblI GTMKAC 1 cut(s) 23
FokI GGATG 1 cut(s) 314
GsuI CTGGAG 1 cut(s) 364
HaeIII GGCC 1 cut(s) 178
HapII CCGG 5 cut(s) 41, 123, 153, 164, 192
HgaI GACGC 2 cut(s) 9, 115
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 3 cut(s) 226, 259, 317
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 2 cut(s) 72, 108
HpaII CCGG 5 cut(s) 41, 123, 153, 164, 192
HphI GGTGA 4 cut(s) 58, 112, 127, 199
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 54, 107, 201
Hpy188III TCNNGA 2 cut(s) 153, 329
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 4 cut(s) 25, 28, 80, 143
HpyAV CCTTC 2 cut(s) 73, 289
HpyCH4III ACNGT 2 cut(s) 139, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 258
HpyF3I CTNAG 1 cut(s) 46
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 3 cut(s) 226, 259, 317
Kpn2I TCCGGA 1 cut(s) 152
Kzo9I GATC 1 cut(s) 49
LweI GCATC 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 133, 187
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MluCI AATT 3 cut(s) 82, 156, 168
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 6 cut(s) 69, 104, 158, 167, 188, 298
MroI TCCGGA 1 cut(s) 152
MseI TTAA 1 cut(s) 159
MspI CCGG 5 cut(s) 41, 123, 153, 164, 192
MspR9I CCNGG 3 cut(s) 41, 165, 343
MvaI CCWGG 1 cut(s) 343
MwoI GCNNNNNNNGC 2 cut(s) 87, 258
NciI CCSGG 2 cut(s) 41, 165
NdeII GATC 1 cut(s) 49
NlaIII CATG 3 cut(s) 226, 259, 317
NlaIV GGNNCC 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 133, 187
PfeI GAWTC 1 cut(s) 108
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 341
PspEI GGTNACC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 176
RsaI GTAC 4 cut(s) 38, 227, 312, 325
RsaNI GTAC 4 cut(s) 37, 226, 311, 324
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 176
ScaI AGTACT 1 cut(s) 325
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 3 cut(s) 41, 165, 343
SetI ASST 4 cut(s) 150, 199, 300, 364
SfaNI GCATC 1 cut(s) 261
SgrAI CRCCGGYG 1 cut(s) 122
SgrDI CGTCGACG 1 cut(s) 22
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 3 cut(s) 82, 156, 168
SsiI CCGC 2 cut(s) 216, 245
StyD4I CCNGG 3 cut(s) 39, 163, 341
TaaI ACNGT 2 cut(s) 139, 278
TaqI TCGA 2 cut(s) 23, 96
TasI AATT 3 cut(s) 82, 156, 168
TatI WGTACW 2 cut(s) 310, 323
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 2 cut(s) 133, 187
Tsp45I GTSAC 2 cut(s) 133, 187
TspDTI ATGAA 3 cut(s) 17, 211, 299
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 1 cut(s) 375
XmiI GTMKAC 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.