pycom05g32560

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011137
Physical Location & Seq
Reverse (-)
31923560 .. 31923949
390 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32560.1

Sequence Viewer

Length: 303 bp
ATGAACACAGACATCACAGCGTCGACGAAGCCCGAGTACCCGGTCATAGATCGGAACCCTCCATTCACCACCGTTGTCGGCAACTTCGGCACCCTCGATTACTTCCGCTTCGCCACCATCACCGGCGTATCCGTCACCGTCGGCTACCTCTCCGGGATTAAGCCGGGGATTAGAGGGCCGTCAATGGTGACCGGAGGTCTGATTGGGGTAATGGGCGGATTCATGTACGCTTATCAGAACTCGGCGGGACGCCTCATGGGCTTTTTCCCCAACGACGATGAGGTAGCTCGTTACAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

101

Amino Acids

10.81

Weight (kDa)

9.05

Isoelectric Point (pI)

14.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADH-u_ox-rdase PF10785 11 - 89 1.1e-19 NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 23
AciI CCGC 3 cut(s) 106, 216, 245
AcyI GRCGYC 1 cut(s) 250
AfaI GTAC 2 cut(s) 38, 227
AhdI GACNNNNNGTC 1 cut(s) 195
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 3 cut(s) 41, 154, 165
AsuHPI GGTGA 4 cut(s) 58, 112, 127, 199
AvaI CYCGRG 1 cut(s) 32
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BanI GGYRCC 1 cut(s) 89
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 163
BciVI GTATCC 1 cut(s) 139
BcnI CCSGG 3 cut(s) 41, 154, 165
BfuI GTATCC 1 cut(s) 139
BglI GCCNNNNNGGC 1 cut(s) 258
Bme1390I CCNGG 3 cut(s) 41, 154, 165
BmeRI GACNNNNNGTC 1 cut(s) 195
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 56, 91
BmrFI CCNGG 3 cut(s) 41, 154, 165
BpuMI CCSGG 3 cut(s) 41, 154, 165
BsaHI GRCGYC 1 cut(s) 250
BsaJI CCNNGG 1 cut(s) 164
BsaWI WCCGGW 1 cut(s) 191
Bse118I RCCGGY 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 164
BshFI GGCC 1 cut(s) 178
BshNI GGYRCC 1 cut(s) 89
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 5 cut(s) 41, 123, 153, 164, 192
BslFI GGGAC 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 32
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 3 cut(s) 106, 216, 245
BspANI GGCC 1 cut(s) 178
BspLI GGNNCC 2 cut(s) 56, 91
BspT107I GGYRCC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 250
Bst4CI ACNGT 2 cut(s) 73, 139
BstACI GRCGYC 1 cut(s) 250
BstEII GGTNACC 1 cut(s) 187
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 2 cut(s) 87, 258
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 39, 152, 163
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 2 cut(s) 9, 258
Csp6I GTAC 2 cut(s) 37, 226
CviAII CATG 2 cut(s) 223, 256
CviJI RGCY 6 cut(s) 31, 144, 163, 178, 261, 287
CviKI_1 RGCY 6 cut(s) 31, 144, 163, 178, 261, 287
CviQI GTAC 2 cut(s) 37, 226
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
FaeI CATG 2 cut(s) 226, 259
FaiI YATR 3 cut(s) 47, 224, 257
FaqI GGGAC 1 cut(s) 261
FatI CATG 2 cut(s) 222, 255
FauI CCCGC 1 cut(s) 238
FblI GTMKAC 1 cut(s) 23
HaeIII GGCC 1 cut(s) 178
HapII CCGG 5 cut(s) 41, 123, 153, 164, 192
HgaI GACGC 2 cut(s) 9, 258
Hin1I GRCGYC 1 cut(s) 250
Hin1II CATG 2 cut(s) 226, 259
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 1 cut(s) 219
HpaII CCGG 5 cut(s) 41, 123, 153, 164, 192
HphI GGTGA 4 cut(s) 58, 112, 127, 199
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 54, 201, 237
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 4 cut(s) 25, 28, 143, 278
HpyCH4III ACNGT 2 cut(s) 73, 139
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 258
Hsp92I GRCGYC 1 cut(s) 250
Hsp92II CATG 2 cut(s) 226, 259
Kzo9I GATC 1 cut(s) 49
LpnPI CCDG 5 cut(s) 54, 136, 166, 177, 205
MaeIII GTNAC 3 cut(s) 133, 187, 290
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MnlI CCTC 7 cut(s) 69, 104, 158, 167, 188, 263, 274
MseI TTAA 1 cut(s) 159
MspI CCGG 5 cut(s) 41, 123, 153, 164, 192
MspR9I CCNGG 3 cut(s) 41, 154, 165
MwoI GCNNNNNNNGC 2 cut(s) 87, 258
NciI CCSGG 3 cut(s) 41, 154, 165
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 226, 259
NlaIV GGNNCC 2 cut(s) 56, 91
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 133, 187
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 1 cut(s) 219
PfoI TCCNGGA 1 cut(s) 152
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 56, 91
PspPI GGNCC 1 cut(s) 176
RsaI GTAC 2 cut(s) 38, 227
RsaNI GTAC 2 cut(s) 37, 226
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 3 cut(s) 41, 154, 165
SetI ASST 4 cut(s) 150, 199, 285, 289
SgrAI CRCCGGYG 1 cut(s) 122
SgrDI CGTCGACG 1 cut(s) 22
SsiI CCGC 3 cut(s) 106, 216, 245
StyD4I CCNGG 3 cut(s) 39, 152, 163
TaaI ACNGT 2 cut(s) 73, 139
TaqI TCGA 2 cut(s) 23, 96
TfiI GAWTC 1 cut(s) 219
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseFI GTSAC 2 cut(s) 133, 187
Tsp45I GTSAC 2 cut(s) 133, 187
TspDTI ATGAA 2 cut(s) 17, 211
TspGWI ACGGA 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.