Rh5AG007100

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011137
Physical Location & Seq
Forward (+)
494271 .. 502185
7915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG007100.1

Sequence Viewer

Length: 348 bp
ATGAACACAGACATCACAGCGTCGACGAAGCCCGAGTACCCTGTCTTAGATCGGAACCCTCCCTTCACCAGAGTCGTCGGCAATTTCAGCACCCTCGACTATTTCCGATTCGCCACCATCACCGGCGTCTCCGTCACCGTCGGCTACCTCTCCGGAATTAAGCCGGGAATTAGAGGGCCGTCTATGGTGACCGGAGGTCTGATTGGAGTGATGGGCGGGTTCATGTACGCTTACCAGAACTCGGCGGGTCGCATCATGGGCTTTTTCCCCAACGACCGTGAGATTCAAATTCCTTTGCATAGCCTTCTAAAACTTGTTACTTCTTCATCTGCAGCCAATCCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

115

Amino Acids

12.37

Weight (kDa)

9.52

Isoelectric Point (pI)

16.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADH-u_ox-rdase PF10785 11 - 89 7.9e-19 NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 152
AciI CCGC 2 cut(s) 216, 245
AcsI RAATTY 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 2 cut(s) 38, 227
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 1 cut(s) 287
AhdI GACNNNNNGTC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 32
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 4 cut(s) 58, 112, 127, 199
AvaI CYCGRG 1 cut(s) 32
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbvI GCAGC 1 cut(s) 344
BccI CCATC 2 cut(s) 125, 205
BceAI ACGGC 1 cut(s) 163
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 133
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
Bme1390I CCNGG 1 cut(s) 165
BmeRI GACNNNNNGTC 1 cut(s) 195
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 165
BsaHI GRCGYC 1 cut(s) 126
BsaWI WCCGGW 2 cut(s) 152, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse118I RCCGGY 1 cut(s) 122
BseAI TCCGGA 1 cut(s) 152
BseLI CCNNNNNNNGG 1 cut(s) 241
BseXI GCAGC 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 1 cut(s) 178
BsiEI CGRYCG 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 4 cut(s) 123, 153, 164, 192
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsnI GGCC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 32
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 2 cut(s) 216, 245
BspANI GGCC 1 cut(s) 178
BspEI TCCGGA 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 56
BspMAI CTGCAG 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 139, 278
BstACI GRCGYC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 46
BstEII GGTNACC 1 cut(s) 187
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 49
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 87, 258
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 1 cut(s) 330
BstV1I GCAGC 1 cut(s) 344
BsuRI GGCC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 2 cut(s) 9, 115
Csp6I GTAC 2 cut(s) 37, 226
CviAII CATG 2 cut(s) 223, 256
CviJI RGCY 7 cut(s) 31, 144, 163, 178, 261, 303, 335
CviKI_1 RGCY 7 cut(s) 31, 144, 163, 178, 261, 303, 335
CviQI GTAC 2 cut(s) 37, 226
DdeI CTNAG 1 cut(s) 46
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 2 cut(s) 226, 259
FaiI YATR 4 cut(s) 185, 224, 257, 300
FatI CATG 2 cut(s) 222, 255
FauI CCCGC 2 cut(s) 209, 238
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 1 cut(s) 333
Fsp4HI GCNGC 1 cut(s) 333
GluI GCNGC 1 cut(s) 333
HaeIII GGCC 1 cut(s) 178
HapII CCGG 4 cut(s) 123, 153, 164, 192
HgaI GACGC 2 cut(s) 9, 115
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 2 cut(s) 226, 259
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 3 cut(s) 72, 108, 283
HpaII CCGG 4 cut(s) 123, 153, 164, 192
HphI GGTGA 4 cut(s) 58, 112, 127, 199
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 54, 107, 201
Hpy188III TCNNGA 1 cut(s) 153
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 4 cut(s) 25, 28, 80, 143
HpyAV CCTTC 2 cut(s) 73, 314
HpyCH4III ACNGT 2 cut(s) 139, 278
HpyCH4V TGCA 2 cut(s) 298, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 258
HpyF3I CTNAG 1 cut(s) 46
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 2 cut(s) 226, 259
Kpn2I TCCGGA 1 cut(s) 152
Kzo9I GATC 1 cut(s) 49
LpnPI CCDG 7 cut(s) 54, 82, 136, 166, 177, 205, 248
Lsp1109I GCAGC 1 cut(s) 344
LweI GCATC 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 133, 187, 316
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 1 cut(s) 315
MluCI AATT 4 cut(s) 82, 156, 168, 288
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 5 cut(s) 69, 104, 158, 167, 188
MroI TCCGGA 1 cut(s) 152
MseI TTAA 1 cut(s) 159
MspI CCGG 4 cut(s) 123, 153, 164, 192
MspR9I CCNGG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 87, 258
NciI CCSGG 1 cut(s) 165
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 226, 259
NlaIV GGNNCC 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 133, 187
PfeI GAWTC 2 cut(s) 108, 283
PkrI GCNGC 1 cut(s) 334
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 1 cut(s) 334
RsaI GTAC 2 cut(s) 38, 227
RsaNI GTAC 2 cut(s) 37, 226
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 1 cut(s) 333
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 165
SetI ASST 2 cut(s) 150, 199
SfaNI GCATC 1 cut(s) 261
SfcI CTRYAG 1 cut(s) 330
SgrAI CRCCGGYG 1 cut(s) 122
SgrDI CGTCGACG 1 cut(s) 22
Sse9I AATT 4 cut(s) 82, 156, 168, 288
SsiI CCGC 2 cut(s) 216, 245
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 2 cut(s) 139, 278
TaqI TCGA 2 cut(s) 23, 96
TasI AATT 4 cut(s) 82, 156, 168, 288
TfiI GAWTC 2 cut(s) 108, 283
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseFI GTSAC 2 cut(s) 133, 187
TseI GCWGC 1 cut(s) 332
Tsp45I GTSAC 2 cut(s) 133, 187
TspDTI ATGAA 3 cut(s) 17, 211, 315
TspGWI ACGGA 1 cut(s) 121
XapI RAATTY 1 cut(s) 288
XmiI GTMKAC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.