Prupe.4G007400_v2.0.a1

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0011137
Physical Location & Seq
Reverse (-)
388411 .. 389223
813 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G007400.1

Sequence Viewer

Length: 303 bp
ATGAACACAGACATCACTGCGTCGACGAACCCCGAGTACCCGGTCATAGATCGGAACCCTCCATTCACCACAGTCGTCGGCAACTTCAGCACCCTCGATTACTTTCGCTTCGCCACCATCACCGGCGTCTCCGTCACCGTAGGCTACCTCTCCGGGATTAAGCCAGGGATTAGAGGGCCGTCTATGGTCACCGGAGGTCTGATTGGGCTAATGGGCGGGTTCATGTACGCTTACCAGAACTCGGCGGGACGCCTCATGGGCTTTTTCCCCAACGACAATGAGGTAGCCCGTTACAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

101

Amino Acids

10.84

Weight (kDa)

9.1

Isoelectric Point (pI)

14.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 23
AciI CCGC 2 cut(s) 216, 245
AcuI CTGAAG 1 cut(s) 70
AcyI GRCGYC 2 cut(s) 126, 250
AfaI GTAC 2 cut(s) 38, 227
AfiI CCNNNNNNNGG 1 cut(s) 241
AjnI CCWGG 1 cut(s) 163
Alw26I GTCTC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 2 cut(s) 41, 154
AsuHPI GGTGA 4 cut(s) 58, 112, 127, 181
AvaI CYCGRG 1 cut(s) 32
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 1 cut(s) 165
BcnI CCSGG 2 cut(s) 41, 154
BcoDI GTCTC 1 cut(s) 133
BglI GCCNNNNNGGC 1 cut(s) 258
Bme1390I CCNGG 3 cut(s) 41, 154, 165
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 3 cut(s) 41, 154, 165
BpuMI CCSGG 2 cut(s) 41, 154
BsaHI GRCGYC 2 cut(s) 126, 250
BsaJI CCNNGG 1 cut(s) 164
BsaWI WCCGGW 1 cut(s) 191
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse118I RCCGGY 1 cut(s) 122
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 241
BshFI GGCC 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 4 cut(s) 41, 123, 153, 192
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 32
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 2 cut(s) 216, 245
BspANI GGCC 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 2 cut(s) 126, 250
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 73, 139
BstACI GRCGYC 2 cut(s) 126, 250
BstEII GGTNACC 1 cut(s) 187
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 2 cut(s) 87, 258
BstNI CCWGG 1 cut(s) 165
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 39, 152, 163
BsuRI GGCC 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 1 cut(s) 15
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 3 cut(s) 9, 115, 258
Csp6I GTAC 2 cut(s) 37, 226
CviAII CATG 2 cut(s) 223, 256
CviJI RGCY 6 cut(s) 144, 163, 178, 208, 261, 287
CviKI_1 RGCY 6 cut(s) 144, 163, 178, 208, 261, 287
CviQI GTAC 2 cut(s) 37, 226
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 163
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 2 cut(s) 226, 259
FaiI YATR 4 cut(s) 47, 185, 224, 257
FaqI GGGAC 1 cut(s) 261
FatI CATG 2 cut(s) 222, 255
FauI CCCGC 2 cut(s) 209, 238
FblI GTMKAC 1 cut(s) 23
HaeIII GGCC 1 cut(s) 178
HapII CCGG 4 cut(s) 41, 123, 153, 192
HgaI GACGC 3 cut(s) 9, 115, 258
Hin1I GRCGYC 2 cut(s) 126, 250
Hin1II CATG 2 cut(s) 226, 259
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HpaII CCGG 4 cut(s) 41, 123, 153, 192
HphI GGTGA 4 cut(s) 58, 112, 127, 181
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 2 cut(s) 54, 201
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 3 cut(s) 25, 28, 80
HpyCH4III ACNGT 2 cut(s) 73, 139
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 258
Hsp92I GRCGYC 2 cut(s) 126, 250
Hsp92II CATG 2 cut(s) 226, 259
Kzo9I GATC 1 cut(s) 49
LpnPI CCDG 7 cut(s) 54, 136, 150, 166, 177, 205, 248
MaeIII GTNAC 3 cut(s) 133, 187, 290
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MnlI CCTC 7 cut(s) 69, 104, 158, 167, 188, 263, 274
MseI TTAA 1 cut(s) 159
MspI CCGG 4 cut(s) 41, 123, 153, 192
MspR9I CCNGG 3 cut(s) 41, 154, 165
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 87, 258
NciI CCSGG 2 cut(s) 41, 154
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 226, 259
NlaIV GGNNCC 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 133, 187
PfoI TCCNGGA 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 163
PspEI GGTNACC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 176
PsrI GAACNNNNNNTAC 2 cut(s) 20, 52
RsaI GTAC 2 cut(s) 38, 227
RsaNI GTAC 2 cut(s) 37, 226
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 3 cut(s) 41, 154, 165
SetI ASST 3 cut(s) 150, 199, 285
SgrAI CRCCGGYG 1 cut(s) 122
SgrDI CGTCGACG 1 cut(s) 22
SsiI CCGC 2 cut(s) 216, 245
StyD4I CCNGG 3 cut(s) 39, 152, 163
TaaI ACNGT 2 cut(s) 73, 139
TaqI TCGA 2 cut(s) 23, 96
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 22
TseFI GTSAC 2 cut(s) 133, 187
Tsp45I GTSAC 2 cut(s) 133, 187
TspDTI ATGAA 2 cut(s) 17, 211
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.