Rh5DG007700

NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011137
Physical Location & Seq
Forward (+)
495733 .. 504042
8310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG007700.1

Sequence Viewer

Length: 336 bp
ATGAACACAGACATCACAGCATCTACGAAGCCCGAGTACCCGGTGTTAGATCGGAACCCTCCCTTCACCAGAGTCGTCGGCAATTTCAGCACCCTCGACTATTTCCGATTCGCCACCATCACCGGCGTCTCCGTCACCGTCGGCTACCTCTCCGGGATTAAGCCGGGAATTAGAGGGCCGTCTATGGTGACCGGAGGTCTGATTGGAGTGATGGGCGGGTTCATGTACGCTTACCAGAACTCGGCGGGTCGCATCATGGGCTTTTTCCCCAACGACCGTGAGACATACTTGTCTCTGATTAAGATTAAGGTGGTTCTCCAGCGATTTCCAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NADH-u_ox-rdase

Protein Analysis

111

Amino Acids

12.22

Weight (kDa)

9.63

Isoelectric Point (pI)

17.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADH-u_ox-rdase PF10785 11 - 89 7e-19 NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 289
AciI CCGC 2 cut(s) 216, 245
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 2 cut(s) 38, 227
AfiI CCNNNNNNNGG 1 cut(s) 241
AhdI GACNNNNNGTC 1 cut(s) 195
Alw26I GTCTC 3 cut(s) 133, 275, 297
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 3 cut(s) 41, 154, 165
AsuHPI GGTGA 4 cut(s) 58, 112, 127, 199
AvaI CYCGRG 1 cut(s) 32
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BccI CCATC 2 cut(s) 125, 205
BceAI ACGGC 1 cut(s) 163
BcnI CCSGG 3 cut(s) 41, 154, 165
BcoDI GTCTC 3 cut(s) 133, 275, 297
Bme1390I CCNGG 3 cut(s) 41, 154, 165
BmeRI GACNNNNNGTC 1 cut(s) 195
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 3 cut(s) 41, 154, 165
BmsI GCATC 2 cut(s) 29, 261
BpmI CTGGAG 1 cut(s) 302
BpuMI CCSGG 3 cut(s) 41, 154, 165
BsaHI GRCGYC 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 191
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse118I RCCGGY 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 241
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 1 cut(s) 178
BsiEI CGRYCG 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 5 cut(s) 41, 123, 153, 164, 192
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 3 cut(s) 133, 275, 297
BsmBI CGTCTC 1 cut(s) 133
BsnI GGCC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 32
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 2 cut(s) 216, 245
BspANI GGCC 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 139, 278
BstACI GRCGYC 1 cut(s) 126
BstEII GGTNACC 1 cut(s) 187
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 3 cut(s) 133, 275, 297
BstMBI GATC 1 cut(s) 49
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 87, 258
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 39, 152, 163
BsuRI GGCC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 37, 226
CviAII CATG 2 cut(s) 223, 256
CviJI RGCY 5 cut(s) 31, 144, 163, 178, 261
CviKI_1 RGCY 5 cut(s) 31, 144, 163, 178, 261
CviQI GTAC 2 cut(s) 37, 226
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DrdI GACNNNNNNGTC 1 cut(s) 289
DriI GACNNNNNGTC 1 cut(s) 195
DseDI GACNNNNNNGTC 1 cut(s) 289
Eam1105I GACNNNNNGTC 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 2 cut(s) 226, 259
FaiI YATR 4 cut(s) 185, 224, 257, 286
FatI CATG 2 cut(s) 222, 255
FauI CCCGC 2 cut(s) 209, 238
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 1 cut(s) 178
HapII CCGG 5 cut(s) 41, 123, 153, 164, 192
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 2 cut(s) 226, 259
HinfI GANTC 2 cut(s) 72, 108
HpaII CCGG 5 cut(s) 41, 123, 153, 164, 192
HphI GGTGA 4 cut(s) 58, 112, 127, 199
Hpy188I TCNGA 4 cut(s) 54, 107, 201, 297
Hpy188III TCNNGA 1 cut(s) 329
Hpy99I CGWCG 2 cut(s) 80, 143
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 2 cut(s) 139, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 258
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 2 cut(s) 226, 259
Kzo9I GATC 1 cut(s) 49
LpnPI CCDG 8 cut(s) 54, 82, 136, 166, 177, 205, 248, 332
LweI GCATC 2 cut(s) 29, 261
MaeIII GTNAC 2 cut(s) 133, 187
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MluCI AATT 2 cut(s) 82, 168
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 5 cut(s) 69, 104, 158, 167, 188
MseI TTAA 3 cut(s) 159, 300, 306
MspI CCGG 5 cut(s) 41, 123, 153, 164, 192
MspR9I CCNGG 3 cut(s) 41, 154, 165
MwoI GCNNNNNNNGC 2 cut(s) 87, 258
NciI CCSGG 3 cut(s) 41, 154, 165
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 226, 259
NlaIV GGNNCC 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 133, 187
PfeI GAWTC 1 cut(s) 108
PfoI TCCNGGA 1 cut(s) 152
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 176
RsaI GTAC 2 cut(s) 38, 227
RsaNI GTAC 2 cut(s) 37, 226
SaqAI TTAA 3 cut(s) 159, 300, 306
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 3 cut(s) 41, 154, 165
SetI ASST 3 cut(s) 150, 199, 312
SfaNI GCATC 2 cut(s) 29, 261
SgrAI CRCCGGYG 1 cut(s) 122
Sse9I AATT 2 cut(s) 82, 168
SsiI CCGC 2 cut(s) 216, 245
StyD4I CCNGG 3 cut(s) 39, 152, 163
TaaI ACNGT 2 cut(s) 139, 278
TaqI TCGA 1 cut(s) 96
TasI AATT 2 cut(s) 82, 168
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 3 cut(s) 159, 300, 306
Tru9I TTAA 3 cut(s) 159, 300, 306
TseFI GTSAC 2 cut(s) 133, 187
Tsp45I GTSAC 2 cut(s) 133, 187
TspDTI ATGAA 2 cut(s) 17, 211
TspGWI ACGGA 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.