Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0004714

Physical Location & Seq

Genomic Coordinates Forward (+)

793541 .. 799457

Gene Structure 5917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g01550.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2685 bp

ATGGAACTGTTAGGAAGGCCGGAACAATATACCGATGAAGATATTGGTTCTGGTGAGAAGGGATTCTCGGAGTTACTAGAGCATGAAAAGGAAGAGTTACTGCAGGACCAATCAGATGAGGATGGGAAGGACAATGGTGGCTTACAGAGGATTGGGGCAAAGTCTTTGTCTAAGAAGAGACGGTGTACTTCAGAAAATGGATCTTCTAATGGAAAACGAATTAGGAAAAAACAACTTGTGCTAAATTATTACGAAGATGAAGATGAGTTGATGGATGAAATGGAGCGTGATACAGAGATTGCCTTTATGATCAAGTCAAAAACACGGAAAAGGGGAAGTCCTGGCCGGGGAAGGATCAACCGGAGTTCCTCAGTGGATTCAGGAGTTAAAGATGAGTCAAAGAATACTATTTCTTACACTTCATCTTCTTCACCACCATCTTCTCCGAGTTCATCAGTTTCAGTTTTAAGATGGGAACGTGGTAGATGCACTTCAAGACACAGAAAGAATAATGGGGATGTTTGTTTTAAGTGTCATCAGTGCATGCAAGAATCGAAAACTATTGTTTCTTGCTCCAAGTGTAAAACCAACACTTATTGTATCCGTTGCATCAAGGAATGGTACCCTCACATGAAGGTACAAGACGTCAAGGAATTTTGCCCATTTTGCCGCCAAAATTGCAACTGTAATGCATGTCTCCACTCAACTGGCATGATTAAGACATCAAAACGGGACTTAAGTGACTATGAAAAGACTCAGCATTTGACGCACTTAATTCTCTCCGTGCTTCCATTTCTGAAAGAATTTTCTGATGAACAAAAGGAGGAGATAAAGATTGAAGCTAATATTCAACGAGAGTCGCCTTCCAACATTATTATAGAACAGACCCTCTGTTTTAATGATGAGCGTGTCTATTGCAACCATTGTGCAACTTCAATTGTTGATCTGCATCGAAGCTGCCCTGACCCAACGTGTAAATACGAACTCTGTCTAAGTTGTTGTAGAGAAATTAGGCAGGGATCCCTTTTAAACCGTACAGAGGTGAAGTTTCAATATAGGAATAGGGGTTGTGAGTACATTCATGGTGGAGATCCATCACGTGAATGTAGTTCTGTACAAGCTCCAGACAATCATATTGAGCCTTTGACTGGGTGGAATGCAAATGATGATAATAGTATTACTTGTGCTCCAAAGGAAATGGGTGGCTGTGGTGAGAGGAAATTGGCTCTGAAGCGAATCCTTCCTTGTGATTGGATTGCAAACTTGGAAGCAAAAGCAAAAACTGTAATAGACATTTGCGAAACCAAAAACTGTACTTTCAAGCATGAATACGGTGAAACAAGAACTGATATGTTGCGAAAAGCAGCTTCGAGGGAGGATTCCTGTGACAACTATTTATATTGTCCAGATTCATGGGACACGCTGAAAGAAGGAGGTCTTTTGCACTTCAGAAAACATTGGATTAATGGTGAACCAGTCATAGTTCAAAATGTACTTGAACAGGCAAATGGTCTGAGCTGGGAGCCAATGGTCATGTGGCGTGCTTTAAGTGAAAATATGGATCCAAATAGTACCTCACAATTTTCAGAGGTCAAGACCATTGATTGCCTGGCTGGCTGTGAGGTGGAAATCAATACTCGGCAATTTTTTGAAGGATACACCGAAGGAAGAATGTATGGAAATTCATGGCCAGAGATGCTTAAGCTGAAGGATTGGCCCCCTTCTGATGCGTTTGAGAATCTTTTGCCCCGACATTGTGATGAATTCATTAGTGCCTTGCCATTTCAAGAGTACACAGATCCTAGAGCTGGTCTTCTTAACCTTGCTGTTAAGTTGCCACCAGGAGTCTTGAAACCAGACATGGGTCCAAAAACATATATTGCTTATGGGCTTGTGGAAGAGCTTGGAAGAGGGGACTCTGTAACCAAGCTGCATTGTGACATGTCTGATGCGGTTAATATTTTGACACATACAGCAGAAATTAAGTTAGGTGATGAGCAGAAATCTGCAATTTCAGACCTAAAAAAATTACATAGGGCCCAAGATGAGAGAGAGCTTCGGAATTGGGTGAATTCTCAGTCTGGACGGTCGGGATCACAAATAGGGGATGATGAAATCATAGAAAAGAATTACAATAGGGAATTCAAGGTTCCAGAAGATCATGCAGAAGGACCCACTCTTTCTGGTTCTCCATGCATGGAAGCAGTAGACGAAACAGGTGGTGGTGCTTTGTGGGACATTTTCCGCAGGGAAGATGTCCCTAAGTTGGAGGCTTATCTGAAGAAGCACGTTAAAGAATTCAGGCACACTTATTGTTCATTAGTTGAACGGGTTATTCATCCAATTCATGATCAATCCTTTTATTTAACAGTGGATCACAAAAGGAAGCTTAAGGAAGAATTTGGAATTGAACCGTGGACATTTGTACAAAGCCTTGGAGAGGCTGTATTTATACCTGCTGGATGCCCGCACCAAGTTAGAAATCTCAAGTCTTGTACAAAAGTAGCCGTGGACTTTGTATCCCCAGAAAATGTCCATGAGTGCCTCCGTTTAACTAAGGACTTCAGACAACTTCCCAAGAATCATAGAGCTAGAGAGGACAAACTTGAGATAAAGAAAATGATACTGTATGCAGTCGACCATGCTGTTAAAGATTTAGAAGCCTTGGTAGCAACTCAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0033169 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04714 map04714

Pfam Domains

Protein Families

JmjC zf-4CXXC_R1

Protein Analysis

895

Amino Acids

102.02

Weight (kDa)

5.98

Isoelectric Point (pI)

49.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JmjC	PF02373	740 - 840	4.8e-14	JmjC domain, hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004714)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11950 AT1G11950 AT1G11950 AT1G62310
fragaria_vesca	FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550 FvH4_3g01550
malus_domestica	MD05G1351300.v1.1 MD10G1325700.v1.1
prunus_persica	Prupe.4G016300_v2.0.a1
pyrus_communis	pycom05g31840 pycom10g27540
rosa_chinensis	RchiOBHm_Chr5g0002371
rosa_laevigata	RLG00000031023
rosa_multiflora	Rmu_co8351855.1_g000001 Rmu_co8445921.1_g000001 Rmu_sc0006137.1_g000004 Rmu_sc0006137.1_g000005 Rmu_sc0017945.1_g000004
rosa_rugosa	Rorug04G0395600 Rorug04G0395700.1
rosa_samantha	Rh5AG018400 Rh5BG021100 Rh5CG020300 Rh5DG019300
rosa_wichuraiana	Rw5G001730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	648
Acc36I	ACCTGC	1 cut(s)	2464
Acc65I	GGTACC	1 cut(s)	621
AccB1I	GGYRCC	1 cut(s)	621
AccI	GTMKAC	2 cut(s)	2208, 2637
AciI	CCGC	4 cut(s)	670, 1952, 2245, 2468
AcoI	YGGCCR	2 cut(s)	343, 1688
AcsI	RAATTY	8 cut(s)	653, 803, 1681, 1763, 2071, 2141, 2297, 2399
AcuI	CTGAAG	6 cut(s)	174, 1250, 1432, 1727, 2300, 2548
AcvI	CACGTG	1 cut(s)	1100
AcyI	GRCGYC	1 cut(s)	645
AfiI	CCNNNNNNNGG	7 cut(s)	347, 1039, 1148, 1808, 1862, 2266, 2440
AflII	CTTAAG	3 cut(s)	736, 1700, 2390
AflIII	ACRYGT	2 cut(s)	971, 1941
AjnI	CCWGG	3 cut(s)	340, 1608, 1840
Alw21I	GWGCWC	1 cut(s)	1189
Alw26I	GTCTC	2 cut(s)	172, 701
AoxI	GGCC	5 cut(s)	17, 343, 1688, 1715, 2037
ApaI	GGGCCC	1 cut(s)	2041
ApeKI	GCWGC	3 cut(s)	957, 1364, 1930
ApoI	RAATTY	8 cut(s)	653, 803, 1681, 1763, 2071, 2141, 2297, 2399
ArsI	GACNNNNNNTTYG	4 cut(s)	152, 184, 745, 777
AseI	ATTAAT	1 cut(s)	1464
Asp700I	GAANNNNTTC	1 cut(s)	62
Asp718I	GGTACC	1 cut(s)	621
AspS9I	GGNCC	6 cut(s)	106, 1716, 1865, 2037, 2038, 2171
AsuC2I	CCSGG	1 cut(s)	347
AsuHPI	GGTGA	8 cut(s)	65, 423, 1054, 1223, 1346, 1481, 2003, 2080
AvaII	GGWCC	3 cut(s)	106, 1865, 2171
BaeGI	GKGCMC	1 cut(s)	2041
BalI	TGGCCA	1 cut(s)	1690
BamHI	GGATCC	2 cut(s)	1019, 1561
BanI	GGYRCC	1 cut(s)	621
BanII	GRGCYC	1 cut(s)	2041
BarI	GAAGNNNNNNTAC	2 cut(s)	475, 507
BbrPI	CACGTG	1 cut(s)	1100
BbsI	GAAGAC	1 cut(s)	1805
Bbv12I	GWGCWC	1 cut(s)	1189
BbvI	GCAGC	3 cut(s)	944, 1376, 1917
BccI	CCATC	5 cut(s)	116, 265, 445, 465, 1102
BceAI	ACGGC	1 cut(s)	2492
BciT130I	CCWGG	3 cut(s)	342, 1610, 1842
BciVI	GTATCC	3 cut(s)	611, 1649, 2530
BclI	TGATCA	2 cut(s)	309, 2350
BcnI	CCSGG	1 cut(s)	347
BcoDI	GTCTC	2 cut(s)	172, 701
BfaI	CTAG	3 cut(s)	77, 1803, 2592
BfmI	CTRYAG	1 cut(s)	101
BfrI	CTTAAG	3 cut(s)	736, 1700, 2390
BfuAI	ACCTGC	1 cut(s)	2464
BfuI	GTATCC	3 cut(s)	611, 1649, 2530
BglI	GCCNNNNNGGC	1 cut(s)	1614
BisI	GCNGC	4 cut(s)	670, 958, 1365, 1931
BlsI	GCNGC	4 cut(s)	671, 959, 1366, 1932
Bme1390I	CCNGG	4 cut(s)	342, 347, 1610, 1842
Bme18I	GGWCC	3 cut(s)	106, 1865, 2171
BmgT120I	GGNCC	6 cut(s)	106, 1716, 1865, 2037, 2038, 2171
BmiI	GGNNCC	9 cut(s)	623, 1021, 1524, 1563, 1718, 1866, 2039, 2151, 2173
BmrFI	CCNGG	4 cut(s)	342, 347, 1610, 1842
BmrI	ACTGGG	1 cut(s)	1158
BmsI	GCATC	7 cut(s)	476, 618, 958, 1686, 1717, 1939, 2453
BmuI	ACTGGG	1 cut(s)	1158
BoxI	GACNNNNGTC	1 cut(s)	1863
BpiI	GAAGAC	1 cut(s)	1805
BpmI	CTGGAG	1 cut(s)	1107
BpuEI	CTTGAG	3 cut(s)	2471, 2627, 2661
BpuMI	CCSGG	1 cut(s)	347
BsaAI	YACGTR	1 cut(s)	1100
BsaBI	GATNNNNATC	1 cut(s)	948
BsaHI	GRCGYC	1 cut(s)	645
BsaJI	CCNNGG	5 cut(s)	346, 2414, 2434, 2508, 2664
BsaWI	WCCGGW	1 cut(s)	360
BsaXI	ACNNNNNCTCC	4 cut(s)	1171, 1201, 2430, 2460
Bsc4I	CCNNNNNNNGG	7 cut(s)	347, 1039, 1148, 1808, 1862, 2266, 2440
Bse1I	ACTGG	3 cut(s)	712, 1153, 1475
Bse8I	GATNNNNATC	1 cut(s)	948
BseBI	CCWGG	3 cut(s)	342, 1610, 1842
BseDI	CCNNGG	5 cut(s)	346, 2414, 2434, 2508, 2664
BseGI	GGATG	6 cut(s)	127, 280, 523, 2113, 2338, 2468
BseJI	GATNNNNATC	1 cut(s)	948
BseLI	CCNNNNNNNGG	7 cut(s)	347, 1039, 1148, 1808, 1862, 2266, 2440
BseMII	CTCAG	4 cut(s)	384, 770, 1505, 2090
BseNI	ACTGG	3 cut(s)	712, 1153, 1475
BseRI	GAGGAG	1 cut(s)	839
BseSI	GKGCMC	1 cut(s)	2041
BseXI	GCAGC	3 cut(s)	944, 1376, 1917
BseYI	CCCAGC	1 cut(s)	1518
Bsh1285I	CGRYCG	1 cut(s)	2090
BshFI	GGCC	5 cut(s)	19, 345, 1690, 1717, 2039
BshNI	GGYRCC	1 cut(s)	621
BsiEI	CGRYCG	1 cut(s)	2090
BsiHKAI	GWGCWC	1 cut(s)	1189
BsiSI	CCGG	3 cut(s)	20, 346, 361
BslFI	GGGAC	5 cut(s)	746, 1430, 1928, 2243, 2249
BslI	CCNNNNNNNGG	7 cut(s)	347, 1039, 1148, 1808, 1862, 2266, 2440
BsmAI	GTCTC	2 cut(s)	172, 701
BsmBI	CGTCTC	1 cut(s)	172
BsmFI	GGGAC	5 cut(s)	746, 1430, 1928, 2243, 2249
BsmI	GAATGC	1 cut(s)	1162
BsnI	GGCC	5 cut(s)	19, 345, 1690, 1717, 2039
Bsp120I	GGGCCC	1 cut(s)	2037
Bsp1286I	GDGCHC	2 cut(s)	1189, 2041
Bsp1407I	TGTACA	3 cut(s)	1114, 2425, 2495
BspACI	CCGC	4 cut(s)	670, 1952, 2245, 2468
BspANI	GGCC	5 cut(s)	19, 345, 1690, 1717, 2039
BspCNI	CTCAG	4 cut(s)	383, 769, 1506, 2089
BspHI	TCATGA	1 cut(s)	2347
BspLI	GGNNCC	9 cut(s)	623, 1021, 1524, 1563, 1718, 1866, 2039, 2151, 2173
BspMAI	CTGCAG	1 cut(s)	105
BspMI	ACCTGC	1 cut(s)	2464
BspQI	GCTCTTC	1 cut(s)	1893
BspT107I	GGYRCC	1 cut(s)	621
BspTI	CTTAAG	3 cut(s)	736, 1700, 2390
BsrGI	TGTACA	3 cut(s)	1114, 2425, 2495
BsrI	ACTGG	3 cut(s)	712, 1153, 1475
BssECI	CCNNGG	5 cut(s)	346, 2414, 2434, 2508, 2664
BssNI	GRCGYC	1 cut(s)	645
BssT1I	CCWWGG	2 cut(s)	2434, 2664
Bst2UI	CCWGG	3 cut(s)	342, 1610, 1842
Bst6I	CTCTTC	4 cut(s)	87, 170, 1893, 1903
BstACI	GRCGYC	1 cut(s)	645
BstAFI	CTTAAG	3 cut(s)	736, 1700, 2390
BstAUI	TGTACA	3 cut(s)	1114, 2425, 2495
BstBAI	YACGTR	1 cut(s)	1100
BstC8I	GCNNGC	4 cut(s)	545, 1542, 1615, 2468
BstDEI	CTNAG	8 cut(s)	171, 370, 756, 992, 1514, 2076, 2262, 2556
BstDSI	CCRYGG	2 cut(s)	2414, 2508
BstENI	CCTNNNNNAGG	1 cut(s)	2438
BstF5I	GGATG	6 cut(s)	127, 280, 523, 2113, 2338, 2468
BstMAI	GTCTC	2 cut(s)	172, 701
BstMCI	CGRYCG	1 cut(s)	2090
BstMWI	GCNNNNNNNGC	6 cut(s)	666, 678, 766, 1614, 1696, 2669
BstNI	CCWGG	3 cut(s)	342, 1610, 1842
BstNSI	RCATGY	3 cut(s)	547, 696, 1945
BstPAI	GACNNNNGTC	1 cut(s)	1863
BstSCI	CCNGG	4 cut(s)	340, 345, 1608, 1840
BstSFI	CTRYAG	1 cut(s)	101
BstSLI	GKGCMC	1 cut(s)	2041
BstV1I	GCAGC	3 cut(s)	944, 1376, 1917
BstV2I	GAAGAC	1 cut(s)	1805
BstX2I	RGATCY	5 cut(s)	200, 1019, 1090, 1561, 1798
BstXI	CCANNNNNNTGG	2 cut(s)	707, 1413
BstYI	RGATCY	5 cut(s)	200, 1019, 1090, 1561, 1798
BsuI	GTATCC	3 cut(s)	611, 1649, 2530
BsuRI	GGCC	5 cut(s)	19, 345, 1690, 1717, 2039
BtgI	CCRYGG	2 cut(s)	2414, 2508
BtsCI	GGATG	6 cut(s)	127, 280, 523, 2113, 2338, 2468
BtsIMutI	CAGTG	3 cut(s)	378, 545, 2376
BveI	ACCTGC	1 cut(s)	2464
Cac8I	GCNNGC	4 cut(s)	545, 1542, 1615, 2468
CciI	TCATGA	1 cut(s)	2347
Cfr13I	GGNCC	6 cut(s)	106, 1716, 1865, 2037, 2038, 2171
CseI	GACGC	1 cut(s)	775
DdeI	CTNAG	8 cut(s)	171, 370, 756, 992, 1514, 2076, 2262, 2556
DraI	TTTAAA	1 cut(s)	1029
EaeI	YGGCCR	2 cut(s)	343, 1688
Eam1104I	CTCTTC	4 cut(s)	87, 170, 1893, 1903
EarI	CTCTTC	4 cut(s)	87, 170, 1893, 1903
Eco130I	CCWWGG	2 cut(s)	2434, 2664
Eco24I	GRGCYC	1 cut(s)	2041
Eco47I	GGWCC	3 cut(s)	106, 1865, 2171
Eco57I	CTGAAG	6 cut(s)	174, 1250, 1432, 1727, 2300, 2548
Eco72I	CACGTG	1 cut(s)	1100
EcoNI	CCTNNNNNAGG	1 cut(s)	2438
EcoO109I	RGGNCCY	2 cut(s)	2037, 2171
EcoRI	GAATTC	4 cut(s)	1763, 2071, 2141, 2297
EcoRII	CCWGG	3 cut(s)	340, 1608, 1840
EcoT14I	CCWWGG	2 cut(s)	2434, 2664
EcoT22I	ATGCAT	2 cut(s)	694, 2198
EcoT38I	GRGCYC	1 cut(s)	2041
ErhI	CCWWGG	2 cut(s)	2434, 2664
Esp3I	CGTCTC	1 cut(s)	172
FalI	AAGNNNNNCTT	2 cut(s)	1422, 1454
FaqI	GGGAC	5 cut(s)	746, 1430, 1928, 2243, 2249
FauI	CCCGC	1 cut(s)	2475
FbaI	TGATCA	2 cut(s)	309, 2350
FblI	GTMKAC	2 cut(s)	2208, 2637
Fnu4HI	GCNGC	4 cut(s)	670, 958, 1365, 1931
FokI	GGATG	6 cut(s)	134, 287, 530, 2120, 2325, 2475
FriOI	GRGCYC	1 cut(s)	2041
Fsp4HI	GCNGC	4 cut(s)	670, 958, 1365, 1931
FspBI	CTAG	3 cut(s)	77, 1803, 2592
GluI	GCNGC	4 cut(s)	670, 958, 1365, 1931
GsaI	CCCAGC	1 cut(s)	1522
GsuI	CTGGAG	1 cut(s)	1107
HaeIII	GGCC	5 cut(s)	19, 345, 1690, 1717, 2039
HapII	CCGG	3 cut(s)	20, 346, 361
HgaI	GACGC	1 cut(s)	775
Hin1I	GRCGYC	1 cut(s)	645
HincII	GTYRAC	1 cut(s)	2638
HindII	GTYRAC	1 cut(s)	2638
HindIII	AAGCTT	1 cut(s)	2387
HpaII	CCGG	3 cut(s)	20, 346, 361
HphI	GGTGA	8 cut(s)	65, 423, 1054, 1223, 1346, 1481, 2003, 2080
Hpy166II	GTNNAC	7 cut(s)	186, 1472, 1794, 2209, 2418, 2512, 2638
Hpy8I	GTNNAC	7 cut(s)	186, 1472, 1794, 2209, 2418, 2512, 2638
HpyCH4IV	ACGT	5 cut(s)	478, 645, 971, 1099, 2289
HpyF10VI	GCNNNNNNNGC	6 cut(s)	666, 678, 766, 1614, 1696, 2669
HpyF3I	CTNAG	8 cut(s)	171, 370, 756, 992, 1514, 2076, 2262, 2556
HpySE526I	ACGT	5 cut(s)	478, 645, 971, 1099, 2289
Hsp92I	GRCGYC	1 cut(s)	645
KpnI	GGTACC	1 cut(s)	625
Ksp22I	TGATCA	2 cut(s)	309, 2350
LguI	GCTCTTC	1 cut(s)	1893
LmnI	GCTCC	5 cut(s)	283, 578, 1126, 1192, 1522
Lsp1109I	GCAGC	3 cut(s)	944, 1376, 1917
LweI	GCATC	7 cut(s)	476, 618, 958, 1686, 1717, 1939, 2453
MaeI	CTAG	3 cut(s)	77, 1803, 2592
MaeII	ACGT	5 cut(s)	478, 645, 971, 1099, 2289
MaeIII	GTNAC	6 cut(s)	72, 96, 740, 1385, 1921, 1937
MfeI	CAATTG	1 cut(s)	936
MflI	RGATCY	5 cut(s)	200, 1019, 1090, 1561, 1798
MhlI	GDGCHC	2 cut(s)	1189, 2041
MlsI	TGGCCA	1 cut(s)	1690
MluNI	TGGCCA	1 cut(s)	1690
MlyI	GAGTC	5 cut(s)	404, 748, 866, 1854, 1910
MmeI	TCCRAC	2 cut(s)	891, 2247
Mox20I	TGGCCA	1 cut(s)	1690
Mph1103I	ATGCAT	2 cut(s)	694, 2198
MroXI	GAANNNNTTC	1 cut(s)	62
MscI	TGGCCA	1 cut(s)	1690
MslI	CAYNNNNRTG	2 cut(s)	1102, 1758
Msp20I	TGGCCA	1 cut(s)	1690
MspCI	CTTAAG	3 cut(s)	736, 1700, 2390
MspI	CCGG	3 cut(s)	20, 346, 361
MspR9I	CCNGG	4 cut(s)	342, 347, 1610, 1842
MunI	CAATTG	1 cut(s)	936
Mva1269I	GAATGC	1 cut(s)	1162
MvaI	CCWGG	3 cut(s)	342, 1610, 1842
MwoI	GCNNNNNNNGC	6 cut(s)	666, 678, 766, 1614, 1696, 2669
NciI	CCSGG	1 cut(s)	347
NlaIV	GGNNCC	9 cut(s)	623, 1021, 1524, 1563, 1718, 1866, 2039, 2151, 2173
NmeAIII	GCCGAG	1 cut(s)	1618
NmuCI	GTSAC	3 cut(s)	740, 1385, 1937
NsiI	ATGCAT	2 cut(s)	694, 2198
NspI	RCATGY	3 cut(s)	547, 696, 1945
PaeI	GCATGC	1 cut(s)	547
PagI	TCATGA	1 cut(s)	2347
PciI	ACATGT	1 cut(s)	1941
PciSI	GCTCTTC	1 cut(s)	1893
PctI	GAATGC	1 cut(s)	1162
PdmI	GAANNNNTTC	1 cut(s)	62
PfeI	GAWTC	8 cut(s)	63, 377, 551, 1236, 1379, 1409, 1738, 2581
PkrI	GCNGC	4 cut(s)	671, 959, 1366, 1932
PleI	GAGTC	5 cut(s)	403, 748, 865, 1853, 1910
PmaCI	CACGTG	1 cut(s)	1100
PmlI	CACGTG	1 cut(s)	1100
PpsI	GAGTC	5 cut(s)	403, 748, 865, 1853, 1910
Ppu21I	YACGTR	1 cut(s)	1100
PpuMI	RGGWCCY	1 cut(s)	2171
PscI	ACATGT	1 cut(s)	1941
PshAI	GACNNNNGTC	1 cut(s)	1863
PshBI	ATTAAT	1 cut(s)	1464
Psp5II	RGGWCCY	1 cut(s)	2171
Psp6I	CCWGG	3 cut(s)	340, 1608, 1840
PspCI	CACGTG	1 cut(s)	1100
PspFI	CCCAGC	1 cut(s)	1518
PspGI	CCWGG	3 cut(s)	340, 1608, 1840
PspN4I	GGNNCC	9 cut(s)	623, 1021, 1524, 1563, 1718, 1866, 2039, 2151, 2173
PspOMI	GGGCCC	1 cut(s)	2037
PspPI	GGNCC	6 cut(s)	106, 1716, 1865, 2037, 2038, 2171
PspPPI	RGGWCCY	1 cut(s)	2171
PstI	CTGCAG	1 cut(s)	105
PsuI	RGATCY	5 cut(s)	200, 1019, 1090, 1561, 1798
RseI	CAYNNNNRTG	2 cut(s)	1102, 1758
SalI	GTCGAC	1 cut(s)	2636
SapI	GCTCTTC	1 cut(s)	1893
SatI	GCNGC	4 cut(s)	670, 958, 1365, 1931
Sau96I	GGNCC	6 cut(s)	106, 1716, 1865, 2037, 2038, 2171
SchI	GAGTC	5 cut(s)	404, 748, 866, 1854, 1910
ScrFI	CCNGG	4 cut(s)	342, 347, 1610, 1842
SduI	GDGCHC	2 cut(s)	1189, 2041
SfaNI	GCATC	7 cut(s)	476, 618, 958, 1686, 1717, 1939, 2453
SfcI	CTRYAG	1 cut(s)	101
SinI	GGWCC	3 cut(s)	106, 1865, 2171
SmiMI	CAYNNNNRTG	2 cut(s)	1102, 1758
SmlI	CTYRAG	6 cut(s)	736, 1700, 2390, 2486, 2606, 2676
SmoI	CTYRAG	6 cut(s)	736, 1700, 2390, 2486, 2606, 2676
SphI	GCATGC	1 cut(s)	547
SsiI	CCGC	4 cut(s)	670, 1952, 2245, 2468
SspI	AATATT	2 cut(s)	847, 1960
SspMI	CTAG	3 cut(s)	77, 1803, 2592
StyD4I	CCNGG	4 cut(s)	340, 345, 1608, 1840
StyI	CCWWGG	2 cut(s)	2434, 2664
TaiI	ACGT	5 cut(s)	481, 648, 974, 1102, 2292
TaqI	TCGA	4 cut(s)	554, 952, 1370, 2637
TatI	WGTACW	8 cut(s)	185, 1074, 1114, 1313, 1492, 1791, 2425, 2495
TauI	GCSGC	1 cut(s)	672
TfiI	GAWTC	8 cut(s)	63, 377, 551, 1236, 1379, 1409, 1738, 2581
TscAI	CASTG	3 cut(s)	378, 545, 2376
TseFI	GTSAC	3 cut(s)	740, 1385, 1937
TseI	GCWGC	3 cut(s)	957, 1364, 1930
Tsp45I	GTSAC	3 cut(s)	740, 1385, 1937
TspGWI	ACGGA	4 cut(s)	340, 593, 772, 2537
TspRI	CASTG	3 cut(s)	378, 545, 2376
Vha464I	CTTAAG	3 cut(s)	736, 1700, 2390
VpaK11BI	GGWCC	3 cut(s)	106, 1865, 2171
VspI	ATTAAT	1 cut(s)	1464
XagI	CCTNNNNNAGG	1 cut(s)	2438
XapI	RAATTY	8 cut(s)	653, 803, 1681, 1763, 2071, 2141, 2297, 2399
XceI	RCATGY	3 cut(s)	547, 696, 1945
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1533, 1606
XmiI	GTMKAC	2 cut(s)	2208, 2637
XmnI	GAANNNNTTC	1 cut(s)	62
XspI	CTAG	3 cut(s)	77, 1803, 2592
ZraI	GACGTC	1 cut(s)	646
Zsp2I	ATGCAT	2 cut(s)	694, 2198

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.