FvH4_3g12720

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013637
Physical Location & Seq
Forward (+)
7614716 .. 7615839
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g12720.t1

Sequence Viewer

Length: 717 bp
ATGGCGCCTGGTTCTAACGACTGGGAGCAATACTACCAGAAAAGCTTCACTGCTACTCCAATGAATGGAGGAGGGATTATTTCATCAGATTTAACTGTTAATAGTGCTGTTGTGACTGATCAAAGCTTCCCCAGTAGTACCAGCCACAATAATGGAGGTCAGTTAACTCCTACCGGGAGTGTTGGGAAACCAATTCGACGAAGGTCTAGGGTTTCCAAGAAGACTCCCATCACTCTCCTTAACGCCAACGCCAACAACTTTCGTGCTTTGGTGCAACAATTCACAGGCTGCGCCGCTTCTAGCACGCCCAATTCGTTCGGGAACCAAAAGGGTCCGATCAATCTGAGCTTCGGAGTCAACAGCAGTAGTGCAGGTACTAGTTCTGTAGTTATGGCGCCGTTTGGAAATCCTAACTATAGTTACAACCAGCAGCAGCAGCAGCAGCAACAATTGCAGCGGCTAGCACAAGTGCAGCAAGAACAGCACCAACAACGACAACCGCTACTACAACTGCAAGAAAACCATGTGCAACTGCATCAAGAAGAGCAGCAGCGTATGTACCCGTTTGATAATGTTAGTGGTAATGATCATGTGTTTCATTATTCATCGAATTCGAGCAGTTCTGGTTATAATCCTATGCAAACTGGTATGGAGATCGGAGACGGATTTGAGGTCATGGATCAGGAAGATATATCGTTACACGAGTTGCTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

239

Amino Acids

26.19

Weight (kDa)

5.75

Isoelectric Point (pI)

53.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 78 - 99 2.5e-07 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
Acc36I ACCTGC 1 cut(s) 362
AccB1I GGYRCC 2 cut(s) 4, 394
AccB7I CCANNNNNTGG 1 cut(s) 65
AciI CCGC 3 cut(s) 294, 457, 500
AclWI GGATC 1 cut(s) 687
AcsI RAATTY 1 cut(s) 610
AcyI GRCGYC 2 cut(s) 5, 395
AfaI GTAC 3 cut(s) 139, 376, 560
AfiI CCNNNNNNNGG 1 cut(s) 65
AhlI ACTAGT 1 cut(s) 377
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 3 cut(s) 45, 126, 348
AluI AGCT 3 cut(s) 45, 126, 348
Alw26I GTCTC 1 cut(s) 654
AlwI GGATC 1 cut(s) 687
ApoI RAATTY 1 cut(s) 610
Asp700I GAANNNNTTC 1 cut(s) 44
AspLEI GCGC 3 cut(s) 7, 293, 397
AspS9I GGNCC 1 cut(s) 332
AsuC2I CCSGG 1 cut(s) 175
AsuNHI GCTAGC 1 cut(s) 460
AvaII GGWCC 1 cut(s) 332
BanI GGYRCC 2 cut(s) 4, 394
BauI CACGAG 1 cut(s) 701
BbsI GAAGAC 1 cut(s) 227
BccI CCATC 1 cut(s) 236
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 1 cut(s) 9
BclI TGATCA 2 cut(s) 118, 586
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 654
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 4 cut(s) 207, 300, 378, 461
BfmI CTRYAG 2 cut(s) 384, 415
BfoI RGCGCY 2 cut(s) 8, 398
BfuAI ACCTGC 1 cut(s) 362
Bme1390I CCNGG 2 cut(s) 9, 175
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 4 cut(s) 6, 323, 333, 396
BmrFI CCNGG 2 cut(s) 9, 175
BmrI ACTGGG 2 cut(s) 31, 126
BmsI GCATC 1 cut(s) 544
BmtI GCTAGC 1 cut(s) 464
BmuI ACTGGG 2 cut(s) 31, 126
BoxI GACNNNNGTC 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 175
BsaHI GRCGYC 2 cut(s) 5, 395
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse1I ACTGG 3 cut(s) 26, 132, 649
BseBI CCWGG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 3 cut(s) 26, 132, 649
BseRI GAGGAG 1 cut(s) 84
BsgI GTGCAG 2 cut(s) 390, 491
BshNI GGYRCC 2 cut(s) 4, 394
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 1 cut(s) 65
BsmAI GTCTC 1 cut(s) 654
BsmBI CGTCTC 1 cut(s) 654
Bsp143I GATC 5 cut(s) 118, 336, 586, 654, 679
BspACI CCGC 3 cut(s) 294, 457, 500
BspCNI CTCAG 1 cut(s) 336
BspLI GGNNCC 4 cut(s) 6, 323, 333, 396
BspMI ACCTGC 1 cut(s) 362
BspOI GCTAGC 1 cut(s) 464
BspPI GGATC 1 cut(s) 687
BspQI GCTCTTC 1 cut(s) 537
BspT107I GGYRCC 2 cut(s) 4, 394
BsrI ACTGG 3 cut(s) 26, 132, 649
BssMI GATC 5 cut(s) 118, 336, 586, 654, 679
BssNI GRCGYC 2 cut(s) 5, 395
BssSI CACGAG 1 cut(s) 701
Bst2BI CACGAG 1 cut(s) 701
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 97
Bst6I CTCTTC 1 cut(s) 537
BstACI GRCGYC 2 cut(s) 5, 395
BstAPI GCANNNNNTGC 1 cut(s) 451
BstC8I GCNNGC 2 cut(s) 305, 462
BstDEI CTNAG 1 cut(s) 344
BstH2I RGCGCY 2 cut(s) 8, 398
BstHHI GCGC 3 cut(s) 7, 293, 397
BstKTI GATC 5 cut(s) 121, 339, 589, 657, 682
BstMAI GTCTC 1 cut(s) 654
BstMBI GATC 5 cut(s) 118, 336, 586, 654, 679
BstMWI GCNNNNNNNGC 5 cut(s) 436, 439, 442, 451, 481
BstNI CCWGG 1 cut(s) 9
BstPAI GACNNNNGTC 1 cut(s) 202
BstSCI CCNGG 2 cut(s) 7, 173
BstSFI CTRYAG 2 cut(s) 384, 415
BstV2I GAAGAC 1 cut(s) 227
BstXI CCANNNNNNTGG 1 cut(s) 152
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 1 cut(s) 48
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 2 cut(s) 305, 462
CfoI GCGC 3 cut(s) 7, 293, 397
Cfr13I GGNCC 1 cut(s) 332
Csp6I GTAC 3 cut(s) 138, 375, 559
CviAII CATG 3 cut(s) 524, 590, 676
CviJI RGCY 6 cut(s) 45, 126, 144, 288, 348, 460
CviKI_1 RGCY 6 cut(s) 45, 126, 144, 288, 348, 460
CviQI GTAC 3 cut(s) 138, 375, 559
DdeI CTNAG 1 cut(s) 344
DinI GGCGCC 2 cut(s) 6, 396
DpnI GATC 5 cut(s) 120, 338, 588, 656, 681
DpnII GATC 5 cut(s) 118, 336, 586, 654, 679
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco47I GGWCC 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 610
EcoRII CCWGG 1 cut(s) 7
EgeI GGCGCC 2 cut(s) 6, 396
EheI GGCGCC 2 cut(s) 6, 396
Esp3I CGTCTC 1 cut(s) 654
FaeI CATG 3 cut(s) 527, 593, 679
FatI CATG 3 cut(s) 523, 589, 675
FbaI TGATCA 2 cut(s) 118, 586
FspBI CTAG 4 cut(s) 207, 300, 378, 461
GlaI GCGC 3 cut(s) 6, 292, 396
HaeII RGCGCY 2 cut(s) 8, 398
HapII CCGG 1 cut(s) 174
HhaI GCGC 3 cut(s) 7, 293, 397
Hin1I GRCGYC 2 cut(s) 5, 395
Hin1II CATG 3 cut(s) 527, 593, 679
Hin6I GCGC 3 cut(s) 5, 291, 395
HinP1I GCGC 3 cut(s) 5, 291, 395
HincII GTYRAC 2 cut(s) 165, 358
HindII GTYRAC 2 cut(s) 165, 358
HindIII AAGCTT 2 cut(s) 43, 124
HinfI GANTC 2 cut(s) 223, 354
HpaI GTTAAC 1 cut(s) 165
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 2 cut(s) 165, 358
Hpy188I TCNGA 5 cut(s) 88, 336, 345, 353, 659
Hpy188III TCNNGA 3 cut(s) 319, 539, 683
Hpy8I GTNNAC 2 cut(s) 165, 358
Hpy99I CGWCG 1 cut(s) 201
HpyAV CCTTC 1 cut(s) 195
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 8 cut(s) 274, 371, 454, 472, 514, 529, 535, 640
HpyF10VI GCNNNNNNNGC 5 cut(s) 436, 439, 442, 451, 481
HpyF3I CTNAG 1 cut(s) 344
Hsp92I GRCGYC 2 cut(s) 5, 395
Hsp92II CATG 3 cut(s) 527, 593, 679
HspAI GCGC 3 cut(s) 5, 291, 395
KasI GGCGCC 2 cut(s) 4, 394
Ksp22I TGATCA 2 cut(s) 118, 586
KspAI GTTAAC 1 cut(s) 165
Kzo9I GATC 5 cut(s) 118, 336, 586, 654, 679
LguI GCTCTTC 1 cut(s) 537
LmnI GCTCC 1 cut(s) 25
LweI GCATC 1 cut(s) 544
MaeI CTAG 4 cut(s) 207, 300, 378, 461
MaeIII GTNAC 3 cut(s) 112, 419, 696
MalI GATC 5 cut(s) 120, 338, 588, 656, 681
MboI GATC 5 cut(s) 118, 336, 586, 654, 679
MboII GAAGA 3 cut(s) 232, 554, 698
MfeI CAATTG 1 cut(s) 449
MluCI AATT 5 cut(s) 192, 278, 310, 449, 610
Mly113I GGCGCC 2 cut(s) 5, 395
MlyI GAGTC 2 cut(s) 217, 363
MnlI CCTC 4 cut(s) 62, 65, 149, 664
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 5 cut(s) 92, 99, 164, 240, 715
MslI CAYNNNNRTG 1 cut(s) 150
MspA1I CMGCKG 1 cut(s) 457
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 9, 175
MunI CAATTG 1 cut(s) 449
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 5 cut(s) 436, 439, 442, 451, 481
NarI GGCGCC 2 cut(s) 5, 395
NciI CCSGG 1 cut(s) 175
NdeII GATC 5 cut(s) 118, 336, 586, 654, 679
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 3 cut(s) 527, 593, 679
NlaIV GGNNCC 4 cut(s) 6, 323, 333, 396
NmuCI GTSAC 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PdmI GAANNNNTTC 1 cut(s) 44
PflMI CCANNNNNTGG 1 cut(s) 65
PleI GAGTC 2 cut(s) 217, 362
PluTI GGCGCC 2 cut(s) 8, 398
PpsI GAGTC 2 cut(s) 217, 362
PshAI GACNNNNGTC 1 cut(s) 202
PsiI TTATAA 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 4 cut(s) 6, 323, 333, 396
PspPI GGNCC 1 cut(s) 332
RsaI GTAC 3 cut(s) 139, 376, 560
RsaNI GTAC 3 cut(s) 138, 375, 559
RseI CAYNNNNRTG 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 537
SaqAI TTAA 5 cut(s) 92, 99, 164, 240, 715
Sau3AI GATC 5 cut(s) 118, 336, 586, 654, 679
Sau96I GGNCC 1 cut(s) 332
SchI GAGTC 2 cut(s) 217, 363
ScrFI CCNGG 2 cut(s) 9, 175
SetI ASST 7 cut(s) 47, 128, 160, 206, 350, 376, 675
SfaNI GCATC 1 cut(s) 544
SfcI CTRYAG 2 cut(s) 384, 415
SfoI GGCGCC 2 cut(s) 6, 396
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 150
SpeI ACTAGT 1 cut(s) 377
Sse9I AATT 5 cut(s) 192, 278, 310, 449, 610
SsiI CCGC 3 cut(s) 294, 457, 500
SspDI GGCGCC 2 cut(s) 4, 394
SspMI CTAG 4 cut(s) 207, 300, 378, 461
StyD4I CCNGG 2 cut(s) 7, 173
TaaI ACNGT 1 cut(s) 97
TaqI TCGA 3 cut(s) 196, 608, 614
TasI AATT 5 cut(s) 192, 278, 310, 449, 610
TauI GCSGC 2 cut(s) 296, 460
Tru1I TTAA 5 cut(s) 92, 99, 164, 240, 715
Tru9I TTAA 5 cut(s) 92, 99, 164, 240, 715
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 112
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 4 cut(s) 72, 77, 587, 594
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 44
XspI CTAG 4 cut(s) 207, 300, 378, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.