FvH4_3g21850

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014275
Physical Location & Seq
Reverse (-)
14911467 .. 14912292
826 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21850.t1

Sequence Viewer

Length: 615 bp
ATGGGCGAACCCTTAAGAGGCTTCAGCAACGTCATGAGCGACGGCACCAAAGTAGTCACCGAGATCGTGTCCGATCACGGCGCAGTGCACCTCTGCCTCTATCTCATCACCCAGAAAATCCGTGACCACAAGGACATGGTCGTAGGGTTTGACACGGAATGCTCTCTGGTAACAATGGACGACGGCCGTGTCGAACCTCAGGTGGTCCTCATCAAACTCTTCTCCTGCGTCGGGTGTGTGCTTATACGATTGAACCATAATTCGGGTCCTGTGTGCCCTTCGCTGGTTAACTTCTTCATGATGAAGGACATTGTGTTTGTTGGGGTGCATGTCAGGGAGGACGTGAGGATGTTGAAGGAGAATTATGGGGTTGTGGTCAGGAATGTGGTGGAGCTGAGTGAGTTGGCGGCGTATGGGCTTCGAAAGCCGCGGTTTGTTGCTTATGGAGCAAGGGACTTGGCTAGAGATGTCTTGAAGATAGGGCTTGAGCAGAGGCCTCAGAATGTGGCTTGGATGAGCTGGATGGAGGAGTTTCTGACGGCGGAGCAGATTGAATGTGCAACTGTTGATGCATATGTTGCTTACAAAACTGGGAAAAAATTGCTGAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

205

Amino Acids

22.89

Weight (kDa)

6.21

Isoelectric Point (pI)

32.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 38 - 202 1.6e-12 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11770 AT5G06450
fragaria_vesca FvH4_3g21850
prunus_persica Prupe.4G202600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037631
rosa_laevigata RLG00000033791 RLG00000033797 RLG00000034136
rosa_roxburghii Rroxscaffold_1G00043320 Rroxscaffold_1G00043380
rosa_rugosa Rorug05G0166200
rosa_samantha Rh5BG291800 Rh5CG290500 Rh5DG265600 Rh5DG266100 Rh5DG301600
rosa_wichuraiana Rw5G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 1 cut(s) 430
AciI CCGC 4 cut(s) 407, 428, 430, 542
AcoI YGGCCR 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 7
AfiI CCNNNNNNNGG 4 cut(s) 17, 231, 262, 283
AflII CTTAAG 1 cut(s) 13
AgsI TTSAA 4 cut(s) 253, 355, 475, 554
AjiI CACGTC 1 cut(s) 343
AluBI AGCT 3 cut(s) 394, 519, 612
AluI AGCT 3 cut(s) 394, 519, 612
Alw21I GWGCWC 1 cut(s) 90
Alw44I GTGCAC 1 cut(s) 86
AoxI GGCC 2 cut(s) 184, 494
ApaLI GTGCAC 1 cut(s) 86
ApeKI GCWGC 1 cut(s) 609
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 205, 266
AsuHPI GGTGA 2 cut(s) 49, 100
AsuII TTCGAA 1 cut(s) 421
AvaII GGWCC 2 cut(s) 205, 266
AxyI CCTNAGG 1 cut(s) 198
BaeGI GKGCMC 2 cut(s) 90, 278
BanI GGYRCC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 90
BccI CCATC 1 cut(s) 517
BceAI ACGGC 5 cut(s) 58, 94, 171, 199, 555
BfaI CTAG 2 cut(s) 462, 613
BfrI CTTAAG 1 cut(s) 13
BisI GCNGC 3 cut(s) 408, 428, 610
BlpI GCTNAGC 1 cut(s) 605
BlsI GCNGC 3 cut(s) 409, 429, 611
Bme18I GGWCC 2 cut(s) 205, 266
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 2 cut(s) 205, 266
BmiI GGNNCC 2 cut(s) 46, 267
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 1 cut(s) 559
BmuI ACTGGG 1 cut(s) 600
Bpu1102I GCTNAGC 1 cut(s) 605
Bpu14I TTCGAA 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 428
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 231, 262, 283
Bse1I ACTGG 1 cut(s) 595
Bse21I CCTNAGG 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 428
BseGI GGATG 3 cut(s) 354, 519, 528
BseLI CCNNNNNNNGG 4 cut(s) 17, 231, 262, 283
BseMII CTCAG 4 cut(s) 212, 386, 512, 596
BseNI ACTGG 1 cut(s) 595
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 2 cut(s) 90, 278
BseX3I CGGCCG 1 cut(s) 184
Bsh1236I CGCG 1 cut(s) 430
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 186, 496
BshNI GGYRCC 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 90
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 4 cut(s) 17, 231, 262, 283
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 2 cut(s) 186, 496
Bsp119I TTCGAA 1 cut(s) 421
Bsp1286I GDGCHC 2 cut(s) 90, 278
Bsp143I GATC 2 cut(s) 63, 73
Bsp1720I GCTNAGC 1 cut(s) 605
BspACI CCGC 4 cut(s) 407, 428, 430, 542
BspANI GGCC 2 cut(s) 186, 496
BspCNI CTCAG 4 cut(s) 211, 387, 511, 597
BspFNI CGCG 1 cut(s) 430
BspHI TCATGA 2 cut(s) 33, 297
BspLI GGNNCC 2 cut(s) 46, 267
BspT104I TTCGAA 1 cut(s) 421
BspT107I GGYRCC 1 cut(s) 44
BspTI CTTAAG 1 cut(s) 13
BsrI ACTGG 1 cut(s) 595
BssECI CCNNGG 1 cut(s) 428
BssMI GATC 2 cut(s) 63, 73
Bst4CI ACNGT 1 cut(s) 565
Bst6I CTCTTC 1 cut(s) 224
BstAFI CTTAAG 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 578
BstBI TTCGAA 1 cut(s) 421
BstDEI CTNAG 4 cut(s) 198, 395, 498, 605
BstDSI CCRYGG 1 cut(s) 428
BstF5I GGATG 3 cut(s) 354, 519, 528
BstFNI CGCG 1 cut(s) 430
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 2 cut(s) 66, 76
BstMBI GATC 2 cut(s) 63, 73
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 3 cut(s) 424, 446, 578
BstNSI RCATGY 1 cut(s) 332
BstSLI GKGCMC 2 cut(s) 90, 278
BstUI CGCG 1 cut(s) 430
BstZI CGGCCG 1 cut(s) 184
Bsu36I CCTNAGG 1 cut(s) 198
BsuRI GGCC 2 cut(s) 186, 496
BtgI CCRYGG 1 cut(s) 428
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 3 cut(s) 354, 519, 528
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 1 cut(s) 90
CciI TCATGA 2 cut(s) 33, 297
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 205, 266
Cfr42I CCGCGG 1 cut(s) 431
CseI GACGC 1 cut(s) 217
CviAII CATG 4 cut(s) 34, 136, 298, 329
DdeI CTNAG 4 cut(s) 198, 395, 498, 605
DpnI GATC 2 cut(s) 65, 75
DpnII GATC 2 cut(s) 63, 73
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 184
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 1 cut(s) 557
EclXI CGGCCG 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 496
Eco47I GGWCC 2 cut(s) 205, 266
Eco52I CGGCCG 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 7
Eco81I CCTNAGG 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 266
EcoT22I ATGCAT 1 cut(s) 574
FaeI CATG 4 cut(s) 37, 139, 301, 332
FaqI GGGAC 1 cut(s) 467
FatI CATG 4 cut(s) 33, 135, 297, 328
FauNDI CATATG 1 cut(s) 574
Fnu4HI GCNGC 3 cut(s) 408, 428, 610
FokI GGATG 3 cut(s) 361, 526, 535
Fsp4HI GCNGC 3 cut(s) 408, 428, 610
FspBI CTAG 2 cut(s) 462, 613
GlaI GCGC 1 cut(s) 82
GluI GCNGC 3 cut(s) 408, 428, 610
HaeIII GGCC 2 cut(s) 186, 496
HgaI GACGC 1 cut(s) 217
HhaI GCGC 1 cut(s) 83
Hin1II CATG 4 cut(s) 37, 139, 301, 332
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HpaI GTTAAC 1 cut(s) 289
HphI GGTGA 2 cut(s) 49, 100
Hpy166II GTNNAC 2 cut(s) 88, 289
Hpy188I TCNGA 3 cut(s) 73, 501, 537
Hpy188III TCNNGA 4 cut(s) 34, 298, 379, 472
Hpy8I GTNNAC 2 cut(s) 88, 289
Hpy99I CGWCG 3 cut(s) 44, 185, 233
HpyAV CCTTC 3 cut(s) 288, 298, 349
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4IV ACGT 2 cut(s) 30, 342
HpyCH4V TGCA 4 cut(s) 88, 328, 560, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 424, 446, 578
HpyF3I CTNAG 4 cut(s) 198, 395, 498, 605
HpySE526I ACGT 2 cut(s) 30, 342
Hsp92II CATG 4 cut(s) 37, 139, 301, 332
HspAI GCGC 1 cut(s) 81
KspAI GTTAAC 1 cut(s) 289
KspI CCGCGG 1 cut(s) 431
Kzo9I GATC 2 cut(s) 63, 73
LmnI GCTCC 3 cut(s) 391, 446, 544
LweI GCATC 1 cut(s) 559
MaeI CTAG 2 cut(s) 462, 613
MaeII ACGT 2 cut(s) 30, 342
MaeIII GTNAC 3 cut(s) 55, 122, 169
MalI GATC 2 cut(s) 65, 75
MboI GATC 2 cut(s) 63, 73
MboII GAAGA 3 cut(s) 211, 286, 487
MhlI GDGCHC 2 cut(s) 90, 278
MluCI AATT 3 cut(s) 259, 361, 599
Mph1103I ATGCAT 1 cut(s) 574
MseI TTAA 2 cut(s) 14, 288
MspA1I CMGCKG 1 cut(s) 430
MspCI CTTAAG 1 cut(s) 13
Mva1269I GAATGC 1 cut(s) 164
MvnI CGCG 1 cut(s) 430
MwoI GCNNNNNNNGC 3 cut(s) 424, 446, 578
NdeI CATATG 1 cut(s) 574
NdeII GATC 2 cut(s) 63, 73
NlaIII CATG 4 cut(s) 37, 139, 301, 332
NlaIV GGNNCC 2 cut(s) 46, 267
NmuCI GTSAC 2 cut(s) 55, 122
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 1 cut(s) 332
NspV TTCGAA 1 cut(s) 421
PagI TCATGA 2 cut(s) 33, 297
PceI AGGCCT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 189
PctI GAATGC 1 cut(s) 164
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 3 cut(s) 409, 429, 611
PpuMI RGGWCCY 1 cut(s) 266
Psp5II RGGWCCY 1 cut(s) 266
PspN4I GGNNCC 2 cut(s) 46, 267
PspPI GGNCC 2 cut(s) 205, 266
PspPPI RGGWCCY 1 cut(s) 266
PsyI GACNNNGTC 1 cut(s) 137
SacII CCGCGG 1 cut(s) 431
SaqAI TTAA 2 cut(s) 14, 288
SatI GCNGC 3 cut(s) 408, 428, 610
Sau3AI GATC 2 cut(s) 63, 73
Sau96I GGNCC 2 cut(s) 205, 266
SduI GDGCHC 2 cut(s) 90, 278
SetI ASST 8 cut(s) 33, 93, 199, 204, 345, 396, 521, 614
SfaNI GCATC 1 cut(s) 559
Sfr303I CCGCGG 1 cut(s) 431
SfuI TTCGAA 1 cut(s) 421
SgrBI CCGCGG 1 cut(s) 431
SinI GGWCC 2 cut(s) 205, 266
SmlI CTYRAG 2 cut(s) 13, 485
SmoI CTYRAG 2 cut(s) 13, 485
Sse9I AATT 3 cut(s) 259, 361, 599
SseBI AGGCCT 1 cut(s) 496
SsiI CCGC 4 cut(s) 407, 428, 430, 542
SspMI CTAG 2 cut(s) 462, 613
StuI AGGCCT 1 cut(s) 496
TaaI ACNGT 1 cut(s) 565
TaiI ACGT 2 cut(s) 33, 345
TaqI TCGA 2 cut(s) 192, 421
TasI AATT 3 cut(s) 259, 361, 599
TauI GCSGC 2 cut(s) 410, 430
Tru1I TTAA 2 cut(s) 14, 288
Tru9I TTAA 2 cut(s) 14, 288
TscAI CASTG 1 cut(s) 90
TseFI GTSAC 2 cut(s) 55, 122
TseI GCWGC 1 cut(s) 609
Tsp45I GTSAC 2 cut(s) 55, 122
TspDTI ATGAA 2 cut(s) 286, 317
TspGWI ACGGA 2 cut(s) 110, 170
TspRI CASTG 1 cut(s) 90
Tth111I GACNNNGTC 1 cut(s) 137
Vha464I CTTAAG 1 cut(s) 13
VneI GTGCAC 1 cut(s) 86
VpaK11BI GGWCC 2 cut(s) 205, 266
XceI RCATGY 1 cut(s) 332
XspI CTAG 2 cut(s) 462, 613
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.