RLG00000033791

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014275
Physical Location & Seq
Reverse (-)
32236477 .. 32237091
615 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033791

Sequence Viewer

Length: 531 bp
ATGAATGAACCCTTAAGAGGCTTCCCCAACGTCATGAGTGACGGTACCAGAGTAGTCACTGAGATCATCTCCGAACACGGTGCAGTGCACCTCTGCCTCTACCTCATCACCCAGAAAATCCGTGACGAGAAGGACATGGTTGTAGGGTTTGACACGGAATGTTCTCTGGTGGACACGGACGACGGCCGAGTCGAACCTCGGGTGGTCCTCATCAAACTCTTCTCCTGCGTCGGGTGTGTCCTTATAAGATTGAACCATAAGTCAGGCCCTGTGTGCCCTTCGCTGCTCAACTTCTTCATTATGAAGGACATTGTGTTTGTTGGGGTTCATGTCAACGAGGACCCGCGGCTTTTTGCTTATGGAGCAAGGGATTTGGCAAGTGATGTTTTGAAGCTGGGGCTTGAGCAGAGGCCTCAGAAGGTGGCTTGGATGAGCTGGACAGAGGAGTTTCTGACCGCAGAGCAGATCGAGTGTGTAACTGTTGATGCTTATGTTGCTTATAAAGCCGGGAAGAAGTTGCTGAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

177

Amino Acids

19.72

Weight (kDa)

5.38

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11770 AT5G06450
fragaria_vesca FvH4_3g21850
prunus_persica Prupe.4G202600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037631
rosa_laevigata RLG00000033791 RLG00000033797 RLG00000034136
rosa_roxburghii Rroxscaffold_1G00043320 Rroxscaffold_1G00043380
rosa_rugosa Rorug05G0166200
rosa_samantha Rh5BG291800 Rh5CG290500 Rh5DG265600 Rh5DG266100 Rh5DG301600
rosa_wichuraiana Rw5G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 245, 501
AasI GACNNNNNNGTC 1 cut(s) 188
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 1 cut(s) 346
AciI CCGC 3 cut(s) 344, 346, 456
AcoI YGGCCR 1 cut(s) 184
AfaI GTAC 1 cut(s) 46
AfiI CCNNNNNNNGG 2 cut(s) 17, 231
AflII CTTAAG 1 cut(s) 13
AgsI TTSAA 2 cut(s) 253, 391
AluBI AGCT 3 cut(s) 394, 435, 528
AluI AGCT 3 cut(s) 394, 435, 528
Alw21I GWGCWC 1 cut(s) 90
Alw44I GTGCAC 1 cut(s) 86
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 3 cut(s) 184, 265, 410
ApaLI GTGCAC 1 cut(s) 86
ApeKI GCWGC 2 cut(s) 283, 525
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp718I GGTACC 1 cut(s) 44
AspS9I GGNCC 3 cut(s) 205, 266, 340
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 1 cut(s) 100
AvaI CYCGRG 1 cut(s) 198
AvaII GGWCC 2 cut(s) 205, 340
BaeGI GKGCMC 2 cut(s) 90, 278
BaeI ACNNNNGTAYC 2 cut(s) 36, 69
BanI GGYRCC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 270
BceAI ACGGC 1 cut(s) 199
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BcnI CCSGG 1 cut(s) 508
BfaI CTAG 1 cut(s) 529
BfrI CTTAAG 1 cut(s) 13
BisI GCNGC 3 cut(s) 284, 347, 526
BlpI GCTNAGC 1 cut(s) 521
BlsI GCNGC 3 cut(s) 285, 348, 527
Bme1390I CCNGG 1 cut(s) 508
Bme18I GGWCC 2 cut(s) 205, 340
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 3 cut(s) 205, 266, 340
BmiI GGNNCC 2 cut(s) 46, 342
BmrFI CCNGG 1 cut(s) 508
BmsI GCATC 1 cut(s) 475
BplI GAGNNNNNCTC 2 cut(s) 53, 85
Bpu1102I GCTNAGC 1 cut(s) 521
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 508
BsaJI CCNNGG 2 cut(s) 197, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 231
BseDI CCNNGG 2 cut(s) 197, 344
BseGI GGATG 1 cut(s) 435
BseLI CCNNNNNNNGG 2 cut(s) 17, 231
BseMII CTCAG 3 cut(s) 51, 428, 512
BseRI GAGGAG 1 cut(s) 458
BseSI GKGCMC 2 cut(s) 90, 278
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 1 cut(s) 270
BseYI CCCAGC 1 cut(s) 394
BsgI GTGCAG 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 346
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 3 cut(s) 186, 267, 412
BshNI GGYRCC 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 1 cut(s) 507
BslI CCNNNNNNNGG 2 cut(s) 17, 231
BsnI GGCC 3 cut(s) 186, 267, 412
BsoBI CYCGRG 1 cut(s) 198
Bsp1286I GDGCHC 2 cut(s) 90, 278
Bsp143I GATC 2 cut(s) 63, 465
Bsp1720I GCTNAGC 1 cut(s) 521
BspACI CCGC 3 cut(s) 344, 346, 456
BspANI GGCC 3 cut(s) 186, 267, 412
BspCNI CTCAG 3 cut(s) 52, 427, 513
BspFNI CGCG 1 cut(s) 346
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 46, 342
BspT107I GGYRCC 1 cut(s) 44
BspTI CTTAAG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 197, 344
BssMI GATC 2 cut(s) 63, 465
Bst4CI ACNGT 3 cut(s) 44, 80, 481
Bst6I CTCTTC 1 cut(s) 224
BstAFI CTTAAG 1 cut(s) 13
BstDEI CTNAG 3 cut(s) 60, 414, 521
BstDSI CCRYGG 1 cut(s) 344
BstF5I GGATG 1 cut(s) 435
BstFNI CGCG 1 cut(s) 346
BstKTI GATC 2 cut(s) 66, 468
BstMBI GATC 2 cut(s) 63, 465
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 4 cut(s) 273, 362, 494, 503
BstSCI CCNGG 1 cut(s) 506
BstSLI GKGCMC 2 cut(s) 90, 278
BstUI CGCG 1 cut(s) 346
BstV1I GCAGC 1 cut(s) 270
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 3 cut(s) 186, 267, 412
BtgI CCRYGG 1 cut(s) 344
BtsCI GGATG 1 cut(s) 435
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 2 cut(s) 57, 90
CaiI CAGNNNCTG 1 cut(s) 269
CciI TCATGA 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 205, 266, 340
Cfr42I CCGCGG 1 cut(s) 347
CseI GACGC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 45
CviAII CATG 3 cut(s) 34, 136, 329
CviQI GTAC 1 cut(s) 45
DdeI CTNAG 3 cut(s) 60, 414, 521
DpnI GATC 2 cut(s) 65, 467
DpnII GATC 2 cut(s) 63, 465
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 184
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EclXI CGGCCG 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 205, 340
Eco52I CGGCCG 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 198
EcoO109I RGGNCCY 2 cut(s) 266, 340
FaeI CATG 3 cut(s) 37, 139, 332
FaiI YATR 9 cut(s) 35, 137, 245, 258, 302, 330, 360, 492, 501
FatI CATG 3 cut(s) 33, 135, 328
FauI CCCGC 1 cut(s) 351
Fnu4HI GCNGC 3 cut(s) 284, 347, 526
FokI GGATG 1 cut(s) 442
Fsp4HI GCNGC 3 cut(s) 284, 347, 526
FspBI CTAG 1 cut(s) 529
GluI GCNGC 3 cut(s) 284, 347, 526
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 3 cut(s) 186, 267, 412
HapII CCGG 1 cut(s) 507
HgaI GACGC 1 cut(s) 217
Hin1II CATG 3 cut(s) 37, 139, 332
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 1 cut(s) 189
HpaII CCGG 1 cut(s) 507
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 3 cut(s) 88, 172, 334
Hpy188I TCNGA 3 cut(s) 73, 417, 453
Hpy188III TCNNGA 1 cut(s) 34
Hpy8I GTNNAC 3 cut(s) 88, 172, 334
Hpy99I CGWCG 2 cut(s) 185, 233
HpyAV CCTTC 4 cut(s) 124, 288, 298, 412
HpyCH4III ACNGT 3 cut(s) 44, 80, 481
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 2 cut(s) 83, 88
HpyF10VI GCNNNNNNNGC 4 cut(s) 273, 362, 494, 503
HpyF3I CTNAG 3 cut(s) 60, 414, 521
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 3 cut(s) 37, 139, 332
KpnI GGTACC 1 cut(s) 48
KspI CCGCGG 1 cut(s) 347
Kzo9I GATC 2 cut(s) 63, 465
LmnI GCTCC 1 cut(s) 362
LpnPI CCDG 9 cut(s) 61, 125, 152, 238, 249, 282, 380, 421, 520
Lsp1109I GCAGC 1 cut(s) 270
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 529
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 4 cut(s) 38, 55, 122, 475
MalI GATC 2 cut(s) 65, 467
MboI GATC 2 cut(s) 63, 465
MboII GAAGA 3 cut(s) 211, 286, 523
MhlI GDGCHC 2 cut(s) 90, 278
MlyI GAGTC 1 cut(s) 198
MseI TTAA 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 346
MspCI CTTAAG 1 cut(s) 13
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 1 cut(s) 508
MvnI CGCG 1 cut(s) 346
MwoI GCNNNNNNNGC 4 cut(s) 273, 362, 494, 503
NciI CCSGG 1 cut(s) 508
NdeII GATC 2 cut(s) 63, 465
NlaIII CATG 3 cut(s) 37, 139, 332
NlaIV GGNNCC 2 cut(s) 46, 342
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 3 cut(s) 38, 55, 122
PagI TCATGA 1 cut(s) 33
PceI AGGCCT 1 cut(s) 412
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PkrI GCNGC 3 cut(s) 285, 348, 527
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 340
PsiI TTATAA 2 cut(s) 245, 501
Psp5II RGGWCCY 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 46, 342
PspPI GGNCC 3 cut(s) 205, 266, 340
PspPPI RGGWCCY 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 269
RsaI GTAC 1 cut(s) 46
RsaNI GTAC 1 cut(s) 45
SacII CCGCGG 1 cut(s) 347
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 3 cut(s) 284, 347, 526
Sau3AI GATC 2 cut(s) 63, 465
Sau96I GGNCC 3 cut(s) 205, 266, 340
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 1 cut(s) 508
SduI GDGCHC 2 cut(s) 90, 278
SetI ASST 8 cut(s) 33, 93, 105, 199, 396, 423, 437, 530
SfaNI GCATC 1 cut(s) 475
Sfr303I CCGCGG 1 cut(s) 347
SgrBI CCGCGG 1 cut(s) 347
SinI GGWCC 2 cut(s) 205, 340
SmlI CTYRAG 2 cut(s) 13, 401
SmoI CTYRAG 2 cut(s) 13, 401
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 3 cut(s) 344, 346, 456
SspMI CTAG 1 cut(s) 529
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 1 cut(s) 506
TaaI ACNGT 3 cut(s) 44, 80, 481
TaiI ACGT 1 cut(s) 33
TaqI TCGA 2 cut(s) 192, 468
TauI GCSGC 1 cut(s) 349
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 2 cut(s) 64, 90
TseFI GTSAC 3 cut(s) 38, 55, 122
TseI GCWGC 2 cut(s) 283, 525
Tsp45I GTSAC 3 cut(s) 38, 55, 122
TspDTI ATGAA 5 cut(s) 17, 21, 286, 317, 317
TspGWI ACGGA 3 cut(s) 110, 170, 191
TspRI CASTG 2 cut(s) 64, 90
Vha464I CTTAAG 1 cut(s) 13
VneI GTGCAC 1 cut(s) 86
VpaK11BI GGWCC 2 cut(s) 205, 340
XspI CTAG 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.