Prupe.4G202600_v2.0.a1

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014275
Physical Location & Seq
Reverse (-)
12608954 .. 12610012
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G202600.1

Sequence Viewer

Length: 636 bp
ATGGAAATATCTTCCAAACCTGGAGACCCACTGCGAGGCTTCCACAACGTAATGTGCGATGGCACGAAAGTTGTCACCGAGATCGTCTCCGATCACGGCTCGATCCACCTCTGCCTCTACCTCATAACCCAAAACATCCGCGACTTCAAAGACATGGTTGTTGGGTTCGATACAGAATGGACTTTCGTCGACGCAGGCACTGGAAAAATCGAAGCTCGCGTCGTCCTCATCAAGCTCTTCTCCTGCATTGGCTGCGTCCTCATTAGATTGGATCACAAGTCGGGCCCGGTTTGCCCGTCTCTCAAGAAATTTCTCATCATGAAGGATGTGATATTTGTCGGGATCCAGATCAAGGAGGATCTGGTGAAGTTGAGGGAGAATTTTGGGATTGATATTAGGAATGTGGTGGAACTGACTGAGCTGGCTGCCAATGTGCTTCGAAAGCCGCGGCTTTTCGCGTGTGGCCCGAGGGATCTGGCAAGGGAGGTTTTGAAGGTTGGGGTTGAGCAGAGGCCGCAGGATGTGATATGGACGAGCTGGGCTGAGGAGTCGCTTACGCCGGAGCAGATTGAGTGCGCCACCATTGATGCTTATCTTACTTACAAAACTGGGAAGAAGTTGTTGAGCAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

212

Amino Acids

23.74

Weight (kDa)

6.44

Isoelectric Point (pI)

29.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11770 AT5G06450
fragaria_vesca FvH4_3g21850
prunus_persica Prupe.4G202600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037631
rosa_laevigata RLG00000033791 RLG00000033797 RLG00000034136
rosa_roxburghii Rroxscaffold_1G00043320 Rroxscaffold_1G00043380
rosa_rugosa Rorug05G0166200
rosa_samantha Rh5BG291800 Rh5CG290500 Rh5DG265600 Rh5DG266100 Rh5DG301600
rosa_wichuraiana Rw5G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 189
AccII CGCG 4 cut(s) 141, 219, 448, 458
AciI CCGC 4 cut(s) 139, 446, 448, 515
AclWI GGATC 6 cut(s) 97, 279, 337, 350, 366, 480
AcsI RAATTY 2 cut(s) 308, 379
AfiI CCNNNNNNNGG 2 cut(s) 35, 352
AgsI TTSAA 2 cut(s) 148, 493
AjnI CCWGG 1 cut(s) 19
AluBI AGCT 4 cut(s) 215, 235, 421, 537
AluI AGCT 4 cut(s) 215, 235, 421, 537
Alw26I GTCTC 3 cut(s) 18, 91, 303
AlwI GGATC 6 cut(s) 97, 279, 337, 350, 366, 480
AlwNI CAGNNNCTG 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 466
AoxI GGCC 3 cut(s) 283, 463, 512
ApaI GGGCCC 1 cut(s) 287
ApeKI GCWGC 2 cut(s) 252, 425
ApoI RAATTY 2 cut(s) 308, 379
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspLEI GCGC 1 cut(s) 578
AspS9I GGNCC 3 cut(s) 283, 284, 464
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 2 cut(s) 67, 376
AsuII TTCGAA 1 cut(s) 439
AvaI CYCGRG 1 cut(s) 466
BaeGI GKGCMC 1 cut(s) 287
BamHI GGATCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 287
BbvCI CCTCAGC 1 cut(s) 543
BbvI GCAGC 2 cut(s) 239, 412
BccI CCATC 1 cut(s) 53
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 21
BcnI CCSGG 1 cut(s) 287
BcoDI GTCTC 3 cut(s) 18, 91, 303
BisI GCNGC 5 cut(s) 253, 426, 446, 449, 515
BlsI GCNGC 5 cut(s) 254, 427, 447, 450, 516
Bme1390I CCNGG 2 cut(s) 21, 287
BmeT110I CYCGRG 1 cut(s) 466
BmgT120I GGNCC 3 cut(s) 283, 284, 464
BmiI GGNNCC 2 cut(s) 285, 344
BmrFI CCNGG 2 cut(s) 21, 287
BmrI ACTGGG 1 cut(s) 618
BmsI GCATC 1 cut(s) 577
BmuI ACTGGG 1 cut(s) 618
BoxI GACNNNNGTC 1 cut(s) 185
BplI GAGNNNNNCTC 2 cut(s) 71, 103
BpmI CTGGAG 1 cut(s) 42
Bpu10I CCTNAGC 1 cut(s) 543
Bpu14I TTCGAA 1 cut(s) 439
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 287
BsaBI GATNNNNATC 2 cut(s) 347, 591
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 446, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 352
Bse1I ACTGG 2 cut(s) 205, 613
Bse8I GATNNNNATC 2 cut(s) 347, 591
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 2 cut(s) 446, 467
BseGI GGATG 3 cut(s) 135, 331, 526
BseJI GATNNNNATC 2 cut(s) 347, 591
BseLI CCNNNNNNNGG 2 cut(s) 35, 352
BseMII CTCAG 2 cut(s) 408, 534
BseNI ACTGG 2 cut(s) 205, 613
BseRI GAGGAG 1 cut(s) 560
BseSI GKGCMC 1 cut(s) 287
BseXI GCAGC 2 cut(s) 239, 412
BseYI CCCAGC 1 cut(s) 537
Bsh1236I CGCG 4 cut(s) 141, 219, 448, 458
BshFI GGCC 3 cut(s) 285, 465, 514
BsiHKCI CYCGRG 1 cut(s) 466
BsiSI CCGG 2 cut(s) 287, 560
BslI CCNNNNNNNGG 2 cut(s) 35, 352
BsmAI GTCTC 3 cut(s) 18, 91, 303
BsmBI CGTCTC 2 cut(s) 91, 303
BsnI GGCC 3 cut(s) 285, 465, 514
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 466
Bsp119I TTCGAA 1 cut(s) 439
Bsp120I GGGCCC 1 cut(s) 283
Bsp1286I GDGCHC 1 cut(s) 287
Bsp143I GATC 8 cut(s) 81, 91, 102, 271, 342, 348, 358, 472
BspACI CCGC 4 cut(s) 139, 446, 448, 515
BspANI GGCC 3 cut(s) 285, 465, 514
BspCNI CTCAG 2 cut(s) 409, 535
BspFNI CGCG 4 cut(s) 141, 219, 448, 458
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 2 cut(s) 285, 344
BspPI GGATC 6 cut(s) 97, 279, 337, 350, 366, 480
BspQI GCTCTTC 1 cut(s) 242
BspT104I TTCGAA 1 cut(s) 439
BspTNI GGTCTC 1 cut(s) 18
BsrI ACTGG 2 cut(s) 205, 613
BssECI CCNNGG 2 cut(s) 446, 467
BssMI GATC 8 cut(s) 81, 91, 102, 271, 342, 348, 358, 472
Bst2UI CCWGG 1 cut(s) 21
Bst6I CTCTTC 1 cut(s) 242
BstAPI GCANNNNNTGC 1 cut(s) 252
BstBI TTCGAA 1 cut(s) 439
BstC8I GCNNGC 3 cut(s) 196, 217, 423
BstDEI CTNAG 2 cut(s) 417, 543
BstDSI CCRYGG 1 cut(s) 446
BstF5I GGATG 3 cut(s) 135, 331, 526
BstFNI CGCG 4 cut(s) 141, 219, 448, 458
BstHHI GCGC 1 cut(s) 578
BstKTI GATC 8 cut(s) 84, 94, 105, 274, 345, 351, 361, 475
BstMAI GTCTC 3 cut(s) 18, 91, 303
BstMBI GATC 8 cut(s) 81, 91, 102, 271, 342, 348, 358, 472
BstMWI GCNNNNNNNGC 4 cut(s) 252, 291, 442, 514
BstNI CCWGG 1 cut(s) 21
BstPAI GACNNNNGTC 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 19, 285
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 4 cut(s) 141, 219, 448, 458
BstV1I GCAGC 2 cut(s) 239, 412
BstX2I RGATCY 3 cut(s) 342, 358, 472
BstYI RGATCY 3 cut(s) 342, 358, 472
BsuRI GGCC 3 cut(s) 285, 465, 514
BtgI CCRYGG 1 cut(s) 446
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 3 cut(s) 135, 331, 526
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 29, 198
Cac8I GCNNGC 3 cut(s) 196, 217, 423
CaiI CAGNNNCTG 1 cut(s) 200
CciI TCATGA 1 cut(s) 318
CfoI GCGC 1 cut(s) 578
Cfr13I GGNCC 3 cut(s) 283, 284, 464
Cfr42I CCGCGG 1 cut(s) 449
CseI GACGC 3 cut(s) 200, 208, 244
CviAII CATG 2 cut(s) 154, 319
DdeI CTNAG 2 cut(s) 417, 543
DpnI GATC 8 cut(s) 83, 93, 104, 273, 344, 350, 360, 474
DpnII GATC 8 cut(s) 81, 91, 102, 271, 342, 348, 358, 472
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
Eco24I GRGCYC 1 cut(s) 287
Eco31I GGTCTC 1 cut(s) 18
Eco88I CYCGRG 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 19
EcoT38I GRGCYC 1 cut(s) 287
Esp3I CGTCTC 2 cut(s) 91, 303
FaeI CATG 2 cut(s) 157, 322
FaiI YATR 4 cut(s) 125, 155, 320, 529
FatI CATG 2 cut(s) 153, 318
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 5 cut(s) 253, 426, 446, 449, 515
FokI GGATG 3 cut(s) 122, 338, 533
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 5 cut(s) 253, 426, 446, 449, 515
GlaI GCGC 1 cut(s) 577
GluI GCNGC 5 cut(s) 253, 426, 446, 449, 515
GsaI CCCAGC 1 cut(s) 541
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 3 cut(s) 285, 465, 514
HapII CCGG 2 cut(s) 287, 560
HgaI GACGC 3 cut(s) 200, 208, 244
HhaI GCGC 1 cut(s) 578
Hin1II CATG 2 cut(s) 157, 322
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 1 cut(s) 548
HpaII CCGG 2 cut(s) 287, 560
HphI GGTGA 2 cut(s) 67, 376
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 4 cut(s) 304, 319, 340, 346
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 3 cut(s) 191, 194, 224
HpyAV CCTTC 2 cut(s) 316, 487
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 4 cut(s) 252, 291, 442, 514
HpyF3I CTNAG 2 cut(s) 417, 543
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 2 cut(s) 157, 322
HspAI GCGC 1 cut(s) 576
KspI CCGCGG 1 cut(s) 449
Kzo9I GATC 8 cut(s) 81, 91, 102, 271, 342, 348, 358, 472
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 1 cut(s) 562
Lsp1109I GCAGC 2 cut(s) 239, 412
LweI GCATC 1 cut(s) 577
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 73
MalI GATC 8 cut(s) 83, 93, 104, 273, 344, 350, 360, 474
MboI GATC 8 cut(s) 81, 91, 102, 271, 342, 348, 358, 472
MboII GAAGA 3 cut(s) 3, 229, 625
MflI RGATCY 3 cut(s) 342, 358, 472
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 2 cut(s) 308, 379
MlyI GAGTC 1 cut(s) 557
MspA1I CMGCKG 1 cut(s) 448
MspI CCGG 2 cut(s) 287, 560
MspR9I CCNGG 2 cut(s) 21, 287
MvaI CCWGG 1 cut(s) 21
MvnI CGCG 4 cut(s) 141, 219, 448, 458
MwoI GCNNNNNNNGC 4 cut(s) 252, 291, 442, 514
NciI CCSGG 1 cut(s) 287
NdeII GATC 8 cut(s) 81, 91, 102, 271, 342, 348, 358, 472
NlaIII CATG 2 cut(s) 157, 322
NlaIV GGNNCC 2 cut(s) 285, 344
NmuCI GTSAC 1 cut(s) 73
NspV TTCGAA 1 cut(s) 439
PagI TCATGA 1 cut(s) 318
PciSI GCTCTTC 1 cut(s) 242
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 216
PkrI GCNGC 5 cut(s) 254, 427, 447, 450, 516
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
PshAI GACNNNNGTC 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 19
PspFI CCCAGC 1 cut(s) 537
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 2 cut(s) 285, 344
PspOMI GGGCCC 1 cut(s) 283
PspPI GGNCC 3 cut(s) 283, 284, 464
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 3 cut(s) 342, 358, 472
SacII CCGCGG 1 cut(s) 449
SalI GTCGAC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 242
SatI GCNGC 5 cut(s) 253, 426, 446, 449, 515
Sau3AI GATC 8 cut(s) 81, 91, 102, 271, 342, 348, 358, 472
Sau96I GGNCC 3 cut(s) 283, 284, 464
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 2 cut(s) 21, 287
SduI GDGCHC 1 cut(s) 287
SfaNI GCATC 1 cut(s) 577
Sfr303I CCGCGG 1 cut(s) 449
SfuI TTCGAA 1 cut(s) 439
SgrBI CCGCGG 1 cut(s) 449
SgrDI CGTCGACG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 2 cut(s) 308, 379
SsiI CCGC 4 cut(s) 139, 446, 448, 515
StyD4I CCNGG 2 cut(s) 19, 285
TaiI ACGT 1 cut(s) 51
TaqI TCGA 5 cut(s) 101, 168, 189, 210, 439
TasI AATT 2 cut(s) 308, 379
TauI GCSGC 3 cut(s) 448, 451, 517
TscAI CASTG 2 cut(s) 36, 205
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 2 cut(s) 252, 425
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 1 cut(s) 335
TspRI CASTG 2 cut(s) 36, 205
XapI RAATTY 2 cut(s) 308, 379
XmiI GTMKAC 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.