Rh5CG290500

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014275
Physical Location & Seq
Reverse (-)
31960962 .. 31961543
582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG290500.1

Sequence Viewer

Length: 582 bp
ATGAGCGACGGCACCAAAGTAGTCACCGAGCTCGTCTCCGAACACGGTGCAGTACACCTCTGCCTCTACCTCATCACCCAGAAAATCCGTGACGAGAAGGACATGGTTGTAGGGTTTGACACGGAATGTTCTCTGGTGGACACAGACGACGGCCGAGTCGAACCTCGGGTGGTCCTCATCAAACTCTTCTCCTGCGTCGGGTGTGTCCTTATAAGATTGAGCCATAAGTCAGGCCCTGTGTGCCCTTCGCTGCTCAACTTCTTCATGATGAAGGACATTGTGTTTGTTGGGGTTCACGTCAATGAGGACGTGAGGATGTTGAAGGAAATTTATGGGGTTGTTGTTAGGAATGTGGTGGAGCTGAGTGAGCTGGCGGCATATGGGCTTCGAAAGCCGCGGCTTTTTGCTTATGGAGCAAGGGATTTGGCAAGAGATGTTTTGAAGCTGGGGCTTGAGCAGAGGCCTCAGAAGGTGGCTTGGATGAGCTGGACAGAGGAGTTTCTGACCGCGGAGCAGATCGAGTGTGCAACTGTTGATGCTTATGTTGCTTATAAAGCCGGGAAGAAGTTGCTGAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

193

Amino Acids

21.64

Weight (kDa)

6.11

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 32 - 190 6.3e-12 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11770 AT5G06450
fragaria_vesca FvH4_3g21850
prunus_persica Prupe.4G202600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037631
rosa_laevigata RLG00000033791 RLG00000033797 RLG00000034136
rosa_roxburghii Rroxscaffold_1G00043320 Rroxscaffold_1G00043380
rosa_rugosa Rorug05G0166200
rosa_samantha Rh5BG291800 Rh5CG290500 Rh5DG265600 Rh5DG266100 Rh5DG301600
rosa_wichuraiana Rw5G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 212, 552
AasI GACNNNNNNGTC 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 11
AccII CGCG 2 cut(s) 397, 509
AciI CCGC 5 cut(s) 374, 395, 397, 507, 509
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 1 cut(s) 327
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 198
AgsI TTSAA 2 cut(s) 322, 442
AjiI CACGTC 2 cut(s) 298, 310
AluBI AGCT 6 cut(s) 31, 361, 370, 445, 486, 579
AluI AGCT 6 cut(s) 31, 361, 370, 445, 486, 579
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 3 cut(s) 151, 232, 461
ApeKI GCWGC 2 cut(s) 250, 576
ApoI RAATTY 1 cut(s) 327
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
AspS9I GGNCC 2 cut(s) 172, 233
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 2 cut(s) 16, 67
AsuII TTCGAA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 245
BanI GGYRCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 237
BceAI ACGGC 2 cut(s) 25, 166
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 580
BisI GCNGC 5 cut(s) 251, 375, 395, 398, 577
BlpI GCTNAGC 1 cut(s) 572
BlsI GCNGC 5 cut(s) 252, 376, 396, 399, 578
Bme1390I CCNGG 1 cut(s) 559
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 165
BmgBI CACGTC 2 cut(s) 298, 310
BmgT120I GGNCC 2 cut(s) 172, 233
BmiI GGNNCC 1 cut(s) 13
BmrFI CCNGG 1 cut(s) 559
BmsI GCATC 1 cut(s) 526
BplI GAGNNNNNCTC 2 cut(s) 20, 52
Bpu1102I GCTNAGC 1 cut(s) 572
Bpu14I TTCGAA 1 cut(s) 388
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 1 cut(s) 559
BsaJI CCNNGG 3 cut(s) 164, 395, 507
Bsc4I CCNNNNNNNGG 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 164, 395, 507
BseGI GGATG 2 cut(s) 321, 486
BseLI CCNNNNNNNGG 1 cut(s) 198
BseMII CTCAG 3 cut(s) 353, 479, 563
BseRI GAGGAG 1 cut(s) 509
BseSI GKGCMC 1 cut(s) 245
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 237
BseYI CCCAGC 1 cut(s) 445
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 2 cut(s) 397, 509
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 153, 234, 463
BshNI GGYRCC 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 558
BslI CCNNNNNNNGG 1 cut(s) 198
BsmAI GTCTC 1 cut(s) 40
BsmBI CGTCTC 1 cut(s) 40
BsnI GGCC 3 cut(s) 153, 234, 463
BsoBI CYCGRG 1 cut(s) 165
Bsp119I TTCGAA 1 cut(s) 388
Bsp1286I GDGCHC 2 cut(s) 33, 245
Bsp143I GATC 1 cut(s) 516
Bsp1720I GCTNAGC 1 cut(s) 572
BspACI CCGC 5 cut(s) 374, 395, 397, 507, 509
BspANI GGCC 3 cut(s) 153, 234, 463
BspCNI CTCAG 3 cut(s) 354, 478, 564
BspFNI CGCG 2 cut(s) 397, 509
BspHI TCATGA 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 13
BspT104I TTCGAA 1 cut(s) 388
BspT107I GGYRCC 1 cut(s) 11
BssECI CCNNGG 3 cut(s) 164, 395, 507
BssMI GATC 1 cut(s) 516
Bst4CI ACNGT 2 cut(s) 47, 532
Bst6I CTCTTC 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 3 cut(s) 362, 465, 572
BstDSI CCRYGG 2 cut(s) 395, 507
BstF5I GGATG 2 cut(s) 321, 486
BstFNI CGCG 2 cut(s) 397, 509
BstKTI GATC 1 cut(s) 519
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 1 cut(s) 516
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 6 cut(s) 240, 367, 391, 413, 545, 554
BstSCI CCNGG 1 cut(s) 557
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 2 cut(s) 397, 509
BstV1I GCAGC 1 cut(s) 237
BstZI CGGCCG 1 cut(s) 151
BsuRI GGCC 3 cut(s) 153, 234, 463
BtgI CCRYGG 2 cut(s) 395, 507
BtrI CACGTC 2 cut(s) 298, 310
BtsCI GGATG 2 cut(s) 321, 486
Cac8I GCNNGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 236
CciI TCATGA 1 cut(s) 264
Cfr13I GGNCC 2 cut(s) 172, 233
Cfr42I CCGCGG 2 cut(s) 398, 510
CseI GACGC 1 cut(s) 184
Csp6I GTAC 1 cut(s) 53
CviAII CATG 2 cut(s) 103, 265
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 3 cut(s) 362, 465, 572
DpnI GATC 1 cut(s) 518
DpnII GATC 1 cut(s) 516
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
EaeI YGGCCR 1 cut(s) 151
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 31
EclXI CGGCCG 1 cut(s) 151
Eco147I AGGCCT 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 172
Eco52I CGGCCG 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 233
EcoT38I GRGCYC 1 cut(s) 33
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 2 cut(s) 106, 268
FatI CATG 2 cut(s) 102, 264
FauNDI CATATG 1 cut(s) 379
Fnu4HI GCNGC 5 cut(s) 251, 375, 395, 398, 577
FokI GGATG 2 cut(s) 328, 493
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 5 cut(s) 251, 375, 395, 398, 577
FspBI CTAG 1 cut(s) 580
GluI GCNGC 5 cut(s) 251, 375, 395, 398, 577
GsaI CCCAGC 1 cut(s) 449
HaeIII GGCC 3 cut(s) 153, 234, 463
HapII CCGG 1 cut(s) 558
HgaI GACGC 1 cut(s) 184
Hin1II CATG 2 cut(s) 106, 268
HinfI GANTC 1 cut(s) 156
HpaII CCGG 1 cut(s) 558
HphI GGTGA 2 cut(s) 16, 67
Hpy166II GTNNAC 3 cut(s) 55, 139, 295
Hpy188I TCNGA 3 cut(s) 40, 468, 504
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 3 cut(s) 55, 139, 295
Hpy99I CGWCG 3 cut(s) 11, 152, 200
HpyAV CCTTC 5 cut(s) 91, 255, 265, 316, 463
HpyCH4III ACNGT 2 cut(s) 47, 532
HpyCH4IV ACGT 2 cut(s) 297, 309
HpyCH4V TGCA 2 cut(s) 50, 527
HpyF10VI GCNNNNNNNGC 6 cut(s) 240, 367, 391, 413, 545, 554
HpyF3I CTNAG 3 cut(s) 362, 465, 572
HpySE526I ACGT 2 cut(s) 297, 309
Hsp92II CATG 2 cut(s) 106, 268
KspI CCGCGG 2 cut(s) 398, 510
Kzo9I GATC 1 cut(s) 516
LmnI GCTCC 3 cut(s) 358, 413, 511
LpnPI CCDG 9 cut(s) 92, 119, 205, 216, 249, 356, 431, 472, 571
Lsp1109I GCAGC 1 cut(s) 237
LweI GCATC 1 cut(s) 526
MaeI CTAG 1 cut(s) 580
MaeII ACGT 2 cut(s) 297, 309
MaeIII GTNAC 2 cut(s) 22, 89
MalI GATC 1 cut(s) 518
MboI GATC 1 cut(s) 516
MboII GAAGA 3 cut(s) 178, 253, 574
MhlI GDGCHC 2 cut(s) 33, 245
MluCI AATT 1 cut(s) 327
MlyI GAGTC 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 300
MspA1I CMGCKG 2 cut(s) 397, 509
MspI CCGG 1 cut(s) 558
MspR9I CCNGG 1 cut(s) 559
MvnI CGCG 2 cut(s) 397, 509
MwoI GCNNNNNNNGC 6 cut(s) 240, 367, 391, 413, 545, 554
NciI CCSGG 1 cut(s) 559
NdeI CATATG 1 cut(s) 379
NdeII GATC 1 cut(s) 516
NlaIII CATG 2 cut(s) 106, 268
NlaIV GGNNCC 1 cut(s) 13
NmeAIII GCCGAG 1 cut(s) 179
NmuCI GTSAC 2 cut(s) 22, 89
NspV TTCGAA 1 cut(s) 388
PagI TCATGA 1 cut(s) 264
PceI AGGCCT 1 cut(s) 463
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PkrI GCNGC 5 cut(s) 252, 376, 396, 399, 578
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
PsiI TTATAA 2 cut(s) 212, 552
Psp124BI GAGCTC 1 cut(s) 33
PspFI CCCAGC 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 13
PspPI GGNCC 2 cut(s) 172, 233
PstNI CAGNNNCTG 1 cut(s) 236
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 1 cut(s) 33
SacII CCGCGG 2 cut(s) 398, 510
SatI GCNGC 5 cut(s) 251, 375, 395, 398, 577
Sau3AI GATC 1 cut(s) 516
Sau96I GGNCC 2 cut(s) 172, 233
SchI GAGTC 1 cut(s) 165
ScrFI CCNGG 1 cut(s) 559
SduI GDGCHC 2 cut(s) 33, 245
SfaNI GCATC 1 cut(s) 526
Sfr303I CCGCGG 2 cut(s) 398, 510
SfuI TTCGAA 1 cut(s) 388
SgrBI CCGCGG 2 cut(s) 398, 510
SinI GGWCC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 1 cut(s) 327
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 5 cut(s) 374, 395, 397, 507, 509
SspMI CTAG 1 cut(s) 580
SstI GAGCTC 1 cut(s) 33
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 1 cut(s) 557
TaaI ACNGT 2 cut(s) 47, 532
TaiI ACGT 2 cut(s) 300, 312
TaqI TCGA 3 cut(s) 159, 388, 519
TasI AATT 1 cut(s) 327
TatI WGTACW 1 cut(s) 52
TauI GCSGC 3 cut(s) 377, 397, 400
TseFI GTSAC 2 cut(s) 22, 89
TseI GCWGC 2 cut(s) 250, 576
Tsp45I GTSAC 2 cut(s) 22, 89
TspDTI ATGAA 2 cut(s) 253, 284
TspGWI ACGGA 2 cut(s) 77, 137
VpaK11BI GGWCC 1 cut(s) 172
XapI RAATTY 1 cut(s) 327
XspI CTAG 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.