Rh5DG265600

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014275
Physical Location & Seq
Reverse (-)
32082016 .. 32082450
435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG265600.1

Sequence Viewer

Length: 435 bp
ATGCCTGAGTTGAACCTCGGGTGGTCCTCATCAAACTCCTCCTGCGTCGGATGTGTCCTTATAAGATTGAACCATAAGTCAGGCCCTGTGTGCCCTTCGCTGCTCAACTTCTTGATGATGAAGGACATTGTGTTTGTTGGGGTTCACGTCAACGTGGATGTGAGGATGTTGAAGGAGAATTATGGTGTTGTGGTTAGGAATGTAGTGGAGCTGAGTGAATTGGCGGCATATGGGCTTCGAAAGCCGCGACTTTTTGCTTATGGAGCAAGGGATTTGGCAAGAGATGTTTTGAAGATGGGGCTTGAGCAGAGGCCTCAGAAGGTGGCTTGGATGAGCTGGACTCATGAGGAGTTTCTGACCACGGAGCAGATCGAGTATGTAACAATTGATGCTTATGTTGCTTATAAAACTGGGAAGAAGTTGCTGAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

144

Amino Acids

16.28

Weight (kDa)

8.79

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 24 - 142 2.3e-08 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11770 AT5G06450
fragaria_vesca FvH4_3g21850
prunus_persica Prupe.4G202600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037631
rosa_laevigata RLG00000033791 RLG00000033797 RLG00000034136
rosa_roxburghii Rroxscaffold_1G00043320 Rroxscaffold_1G00043380
rosa_rugosa Rorug05G0166200
rosa_samantha Rh5BG291800 Rh5CG290500 Rh5DG265600 Rh5DG266100 Rh5DG301600
rosa_wichuraiana Rw5G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 62, 405
AccII CGCG 1 cut(s) 247
AciI CCGC 2 cut(s) 224, 245
AgsI TTSAA 4 cut(s) 13, 70, 172, 292
AjiI CACGTC 1 cut(s) 148
AluBI AGCT 3 cut(s) 211, 336, 432
AluI AGCT 3 cut(s) 211, 336, 432
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 17
AoxI GGCC 2 cut(s) 82, 311
ApeKI GCWGC 2 cut(s) 100, 429
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AspS9I GGNCC 2 cut(s) 24, 83
AsuII TTCGAA 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 17
AvaII GGWCC 1 cut(s) 24
BaeGI GKGCMC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 87
BccI CCATC 1 cut(s) 289
BfaI CTAG 1 cut(s) 433
BisI GCNGC 4 cut(s) 101, 225, 245, 430
BlpI GCTNAGC 1 cut(s) 425
BlsI GCNGC 4 cut(s) 102, 226, 246, 431
Bme18I GGWCC 1 cut(s) 24
BmeT110I CYCGRG 1 cut(s) 17
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 24, 83
BmrI ACTGGG 1 cut(s) 420
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 420
BplI GAGNNNNNCTC 2 cut(s) 325, 357
Bpu1102I GCTNAGC 1 cut(s) 425
Bpu14I TTCGAA 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 16, 360
Bse1I ACTGG 1 cut(s) 415
BseDI CCNNGG 2 cut(s) 16, 360
BseGI GGATG 4 cut(s) 56, 163, 171, 336
BseMII CTCAG 3 cut(s) 203, 329, 416
BseNI ACTGG 1 cut(s) 415
BseRI GAGGAG 2 cut(s) 28, 362
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 247
BshFI GGCC 2 cut(s) 84, 313
BsiHKCI CYCGRG 1 cut(s) 17
BsnI GGCC 2 cut(s) 84, 313
BsoBI CYCGRG 1 cut(s) 17
Bsp119I TTCGAA 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 1 cut(s) 369
Bsp1720I GCTNAGC 1 cut(s) 425
BspACI CCGC 2 cut(s) 224, 245
BspANI GGCC 2 cut(s) 84, 313
BspCNI CTCAG 3 cut(s) 204, 328, 417
BspFNI CGCG 1 cut(s) 247
BspHI TCATGA 1 cut(s) 343
BspT104I TTCGAA 1 cut(s) 238
BsrI ACTGG 1 cut(s) 415
BssECI CCNNGG 2 cut(s) 16, 360
BssMI GATC 1 cut(s) 369
BstBI TTCGAA 1 cut(s) 238
BstDEI CTNAG 4 cut(s) 6, 212, 315, 425
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 4 cut(s) 56, 163, 171, 336
BstFNI CGCG 1 cut(s) 247
BstKTI GATC 1 cut(s) 372
BstMBI GATC 1 cut(s) 369
BstMWI GCNNNNNNNGC 4 cut(s) 90, 241, 263, 398
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 87
BsuRI GGCC 2 cut(s) 84, 313
BtgI CCRYGG 1 cut(s) 360
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 4 cut(s) 56, 163, 171, 336
CaiI CAGNNNCTG 1 cut(s) 86
CciI TCATGA 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 24, 83
CseI GACGC 1 cut(s) 34
CviAII CATG 1 cut(s) 344
CviJI RGCY 9 cut(s) 84, 211, 235, 244, 301, 313, 326, 336, 432
CviKI_1 RGCY 9 cut(s) 84, 211, 235, 244, 301, 313, 326, 336, 432
DdeI CTNAG 4 cut(s) 6, 212, 315, 425
DpnI GATC 1 cut(s) 371
DpnII GATC 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 83
FaeI CATG 1 cut(s) 347
FatI CATG 1 cut(s) 343
FauNDI CATATG 1 cut(s) 229
Fnu4HI GCNGC 4 cut(s) 101, 225, 245, 430
FokI GGATG 4 cut(s) 63, 170, 178, 343
Fsp4HI GCNGC 4 cut(s) 101, 225, 245, 430
FspBI CTAG 1 cut(s) 433
GluI GCNGC 4 cut(s) 101, 225, 245, 430
HaeIII GGCC 2 cut(s) 84, 313
HgaI GACGC 1 cut(s) 34
Hin1II CATG 1 cut(s) 347
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 1 cut(s) 340
Hpy166II GTNNAC 2 cut(s) 145, 151
Hpy188I TCNGA 3 cut(s) 50, 318, 357
Hpy188III TCNNGA 2 cut(s) 112, 344
Hpy8I GTNNAC 2 cut(s) 145, 151
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 4 cut(s) 105, 115, 166, 313
HpyCH4IV ACGT 2 cut(s) 147, 153
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 241, 263, 398
HpyF3I CTNAG 4 cut(s) 6, 212, 315, 425
HpySE526I ACGT 2 cut(s) 147, 153
Hsp92II CATG 1 cut(s) 347
Kzo9I GATC 1 cut(s) 369
LmnI GCTCC 3 cut(s) 208, 263, 364
LpnPI CCDG 6 cut(s) 18, 55, 66, 99, 322, 396
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 1 cut(s) 379
MaeI CTAG 1 cut(s) 433
MaeII ACGT 2 cut(s) 147, 153
MaeIII GTNAC 1 cut(s) 379
MalI GATC 1 cut(s) 371
MboI GATC 1 cut(s) 369
MboII GAAGA 2 cut(s) 304, 427
MfeI CAATTG 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 95
MluCI AATT 3 cut(s) 178, 218, 384
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 7 cut(s) 26, 37, 49, 156, 303, 324, 340
MunI CAATTG 1 cut(s) 384
MvnI CGCG 1 cut(s) 247
MwoI GCNNNNNNNGC 4 cut(s) 90, 241, 263, 398
NdeI CATATG 1 cut(s) 229
NdeII GATC 1 cut(s) 369
NlaIII CATG 1 cut(s) 347
NspV TTCGAA 1 cut(s) 238
PagI TCATGA 1 cut(s) 343
PceI AGGCCT 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PkrI GCNGC 4 cut(s) 102, 226, 246, 431
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
PsiI TTATAA 2 cut(s) 62, 405
PspPI GGNCC 2 cut(s) 24, 83
PstNI CAGNNNCTG 1 cut(s) 86
SatI GCNGC 4 cut(s) 101, 225, 245, 430
Sau3AI GATC 1 cut(s) 369
Sau96I GGNCC 2 cut(s) 24, 83
SchI GAGTC 1 cut(s) 334
SduI GDGCHC 1 cut(s) 95
SetI ASST 7 cut(s) 18, 150, 156, 213, 324, 338, 434
SfaNI GCATC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 238
SinI GGWCC 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 3 cut(s) 178, 218, 384
SseBI AGGCCT 1 cut(s) 313
SsiI CCGC 2 cut(s) 224, 245
SspMI CTAG 1 cut(s) 433
StuI AGGCCT 1 cut(s) 313
TaiI ACGT 2 cut(s) 150, 156
TaqI TCGA 2 cut(s) 238, 372
TasI AATT 3 cut(s) 178, 218, 384
TauI GCSGC 2 cut(s) 227, 247
TseI GCWGC 2 cut(s) 100, 429
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 24
XspI CTAG 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.