Rw5G023660

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014275
Physical Location & Seq
Reverse (-)
33445705 .. 33446309
605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023660.1

Sequence Viewer

Length: 519 bp
ATGAATGAACCTTTAAGAGGCTTCACCAACGTCATGAGCGACAGCACCAAAGTGGTCACTGAGATCGTCTGCGAACACGGTGCAGTGCACCTCTGCCTCTACCTCATCACCCAGAAAATCCGTGAAGAGAAGGACATGGTTGTAGGGTTTGACAGGGAATGTTCTCTGGTAGACACGGAAGACGGCCGAGTCGAACCTCGGGCAGTCCTTATCAAACTCTTCTCCTGCGTCGGGTGTGTCCTTATAAGATTGAACCATAAGTCAGGCCATGTGTGCCCTTGGCTGCTCAACTTCTTCATGATGAAGGACATTGTTTTTGTTGGAGTTCATGTCAACGAGGACGTGAGGATGTTGAAGGAGAATTATGGGGTTGTGGTTAGGAATGTGGTGGAGCTGCTGGGGCTTGAGCAGAGGCCTCAGAAGGTGGCTTGGATGAGCTGGACTGAGGAGTTTCTGACCGCGGAGCAGATCGAGTGTGTAACAATTGATGCTTATGTTGCTTATAAAACTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

172

Amino Acids

19.68

Weight (kDa)

5.56

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11770 AT5G06450
fragaria_vesca FvH4_3g21850
prunus_persica Prupe.4G202600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037631
rosa_laevigata RLG00000033791 RLG00000033797 RLG00000034136
rosa_roxburghii Rroxscaffold_1G00043320 Rroxscaffold_1G00043380
rosa_rugosa Rorug05G0166200
rosa_samantha Rh5BG291800 Rh5CG290500 Rh5DG265600 Rh5DG266100 Rh5DG301600
rosa_wichuraiana Rw5G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 245, 504
AasI GACNNNNNNGTC 1 cut(s) 188
AccI GTMKAC 1 cut(s) 171
AccII CGCG 1 cut(s) 461
AciI CCGC 2 cut(s) 459, 461
AcoI YGGCCR 1 cut(s) 184
AfiI CCNNNNNNNGG 2 cut(s) 17, 231
AgsI TTSAA 2 cut(s) 253, 355
AjiI CACGTC 1 cut(s) 343
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 2 cut(s) 394, 438
AluI AGCT 2 cut(s) 394, 438
Alw21I GWGCWC 1 cut(s) 90
Alw44I GTGCAC 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 3 cut(s) 184, 265, 413
ApaLI GTGCAC 1 cut(s) 86
ApeKI GCWGC 2 cut(s) 283, 394
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AsuHPI GGTGA 2 cut(s) 16, 100
AvaI CYCGRG 1 cut(s) 198
BaeGI GKGCMC 2 cut(s) 90, 278
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 270, 381
BceAI ACGGC 1 cut(s) 199
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BisI GCNGC 2 cut(s) 284, 395
BlsI GCNGC 2 cut(s) 285, 396
BmeT110I CYCGRG 1 cut(s) 198
BmgBI CACGTC 1 cut(s) 343
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 1 cut(s) 478
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 425
BsaJI CCNNGG 3 cut(s) 197, 278, 459
BsaXI ACNNNNNCTCC 2 cut(s) 315, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 231
Bse1I ACTGG 1 cut(s) 514
BseDI CCNNGG 3 cut(s) 197, 278, 459
BseGI GGATG 2 cut(s) 354, 438
BseLI CCNNNNNNNGG 2 cut(s) 17, 231
BseMII CTCAG 3 cut(s) 51, 431, 435
BseNI ACTGG 1 cut(s) 514
BseRI GAGGAG 1 cut(s) 461
BseSI GKGCMC 2 cut(s) 90, 278
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 2 cut(s) 270, 381
BseYI CCCAGC 1 cut(s) 397
BsgI GTGCAG 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 3 cut(s) 186, 267, 415
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 198
BslI CCNNNNNNNGG 2 cut(s) 17, 231
BsnI GGCC 3 cut(s) 186, 267, 415
BsoBI CYCGRG 1 cut(s) 198
Bsp1286I GDGCHC 2 cut(s) 90, 278
Bsp143I GATC 2 cut(s) 63, 468
BspACI CCGC 2 cut(s) 459, 461
BspANI GGCC 3 cut(s) 186, 267, 415
BspCNI CTCAG 3 cut(s) 52, 430, 436
BspFNI CGCG 1 cut(s) 461
BspHI TCATGA 2 cut(s) 33, 297
BsrI ACTGG 1 cut(s) 514
BssECI CCNNGG 3 cut(s) 197, 278, 459
BssMI GATC 2 cut(s) 63, 468
BssT1I CCWWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 80
Bst6I CTCTTC 2 cut(s) 120, 224
BstDEI CTNAG 3 cut(s) 60, 417, 444
BstDSI CCRYGG 1 cut(s) 459
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 354, 438
BstFNI CGCG 1 cut(s) 461
BstKTI GATC 2 cut(s) 66, 471
BstMBI GATC 2 cut(s) 63, 468
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 3 cut(s) 273, 400, 497
BstSLI GKGCMC 2 cut(s) 90, 278
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 2 cut(s) 270, 381
BstV2I GAAGAC 1 cut(s) 186
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 3 cut(s) 186, 267, 415
BtgI CCRYGG 1 cut(s) 459
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 2 cut(s) 354, 438
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 2 cut(s) 57, 90
CciI TCATGA 2 cut(s) 33, 297
Cfr42I CCGCGG 1 cut(s) 462
CseI GACGC 1 cut(s) 217
CviAII CATG 5 cut(s) 34, 136, 269, 298, 329
CviJI RGCY 9 cut(s) 21, 186, 267, 283, 394, 403, 415, 428, 438
CviKI_1 RGCY 9 cut(s) 21, 186, 267, 283, 394, 403, 415, 428, 438
DdeI CTNAG 3 cut(s) 60, 417, 444
DpnI GATC 2 cut(s) 65, 470
DpnII GATC 2 cut(s) 63, 468
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 184
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 120, 224
EarI CTCTTC 2 cut(s) 120, 224
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 278
Eco147I AGGCCT 1 cut(s) 415
Eco52I CGGCCG 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 198
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 278
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 5 cut(s) 37, 139, 272, 301, 332
FatI CATG 5 cut(s) 33, 135, 268, 297, 328
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 284, 395
FokI GGATG 2 cut(s) 361, 445
Fsp4HI GCNGC 2 cut(s) 284, 395
GluI GCNGC 2 cut(s) 284, 395
GsaI CCCAGC 1 cut(s) 401
HaeIII GGCC 3 cut(s) 186, 267, 415
HgaI GACGC 1 cut(s) 217
Hin1II CATG 5 cut(s) 37, 139, 272, 301, 332
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 1 cut(s) 189
HphI GGTGA 2 cut(s) 16, 100
Hpy166II GTNNAC 3 cut(s) 88, 172, 334
Hpy188I TCNGA 2 cut(s) 420, 456
Hpy188III TCNNGA 2 cut(s) 34, 298
Hpy8I GTNNAC 3 cut(s) 88, 172, 334
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 4 cut(s) 124, 298, 349, 415
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4IV ACGT 2 cut(s) 30, 342
HpyCH4V TGCA 2 cut(s) 83, 88
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 400, 497
HpyF3I CTNAG 3 cut(s) 60, 417, 444
HpySE526I ACGT 2 cut(s) 30, 342
Hsp92II CATG 5 cut(s) 37, 139, 272, 301, 332
KspI CCGCGG 1 cut(s) 462
Kzo9I GATC 2 cut(s) 63, 468
LmnI GCTCC 2 cut(s) 391, 463
LpnPI CCDG 8 cut(s) 125, 139, 152, 238, 249, 383, 424, 495
Lsp1109I GCAGC 2 cut(s) 270, 381
LweI GCATC 1 cut(s) 478
MaeII ACGT 2 cut(s) 30, 342
MaeIII GTNAC 2 cut(s) 55, 478
MalI GATC 2 cut(s) 65, 470
MboI GATC 2 cut(s) 63, 468
MboII GAAGA 4 cut(s) 137, 191, 211, 286
MfeI CAATTG 1 cut(s) 483
MhlI GDGCHC 2 cut(s) 90, 278
MluCI AATT 2 cut(s) 361, 483
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 1 cut(s) 301
MseI TTAA 1 cut(s) 14
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 461
MunI CAATTG 1 cut(s) 483
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 3 cut(s) 273, 400, 497
NdeII GATC 2 cut(s) 63, 468
NlaIII CATG 5 cut(s) 37, 139, 272, 301, 332
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 1 cut(s) 55
OliI CACNNNNGTG 1 cut(s) 50
PagI TCATGA 2 cut(s) 33, 297
PceI AGGCCT 1 cut(s) 415
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 189
PkrI GCNGC 2 cut(s) 285, 396
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
PsiI TTATAA 2 cut(s) 245, 504
PspFI CCCAGC 1 cut(s) 397
RseI CAYNNNNRTG 1 cut(s) 50
SacII CCGCGG 1 cut(s) 462
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 2 cut(s) 284, 395
Sau3AI GATC 2 cut(s) 63, 468
SchI GAGTC 1 cut(s) 198
SduI GDGCHC 2 cut(s) 90, 278
SetI ASST 9 cut(s) 13, 33, 93, 105, 199, 345, 396, 426, 440
SfaNI GCATC 1 cut(s) 478
Sfr303I CCGCGG 1 cut(s) 462
SgrBI CCGCGG 1 cut(s) 462
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 2 cut(s) 361, 483
SseBI AGGCCT 1 cut(s) 415
SsiI CCGC 2 cut(s) 459, 461
StuI AGGCCT 1 cut(s) 415
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 1 cut(s) 80
TaiI ACGT 2 cut(s) 33, 345
TaqI TCGA 2 cut(s) 192, 471
TasI AATT 2 cut(s) 361, 483
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 2 cut(s) 64, 90
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 2 cut(s) 283, 394
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 5 cut(s) 17, 21, 286, 317, 317
TspGWI ACGGA 2 cut(s) 110, 191
TspRI CASTG 2 cut(s) 64, 90
VneI GTGCAC 1 cut(s) 86
XagI CCTNNNNNAGG 1 cut(s) 15
XmiI GTMKAC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.