RLG00000033797

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014275
Physical Location & Seq
Reverse (-)
32352081 .. 32352581
501 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033797

Sequence Viewer

Length: 408 bp
ATGAATGAACCCTTAAGAGGCTTCACCAACGTCATGAGCGACGGCACCAAAGTAGTCACCGAGATCGTCTCCGAACATGACACGGAAGACGGGCGAGTCGAACCTCGGGTGGTCCTTATCAAACTCTTGTCATGCGTCGGGTGTGTCCTTATAAGATTGAACCACAAGTCAGGCCCCGTGTGCCCTTCGCTGCTCAATTTCTTCATGATGAAGGACATTGTTTTTGTTGGAGTTCATGTCAACGAGGACGTGAAGATGTTGAAGGAGAATTATGGCGTTGTGGTTAGGAATGTGGTGGATCTGAGTGAATTGGCGACATATGGGCTTCGAAAGCCGCGGCTTTTTGCTTATGGAGCAAGGGATTTGGCAAGAGATGTTTTGAAGCTGGGGCTTGAGCAGAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

136

Amino Acids

15.16

Weight (kDa)

7.73

Isoelectric Point (pI)

23.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11770 AT5G06450
fragaria_vesca FvH4_3g21850
prunus_persica Prupe.4G202600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037631
rosa_laevigata RLG00000033791 RLG00000033797 RLG00000034136
rosa_roxburghii Rroxscaffold_1G00043320 Rroxscaffold_1G00043380
rosa_rugosa Rorug05G0166200
rosa_samantha Rh5BG291800 Rh5CG290500 Rh5DG265600 Rh5DG266100 Rh5DG301600
rosa_wichuraiana Rw5G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AasI GACNNNNNNGTC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 1 cut(s) 337
AciI CCGC 2 cut(s) 335, 337
AclWI GGATC 1 cut(s) 306
AfiI CCNNNNNNNGG 1 cut(s) 17
AflII CTTAAG 1 cut(s) 13
AgsI TTSAA 3 cut(s) 160, 262, 382
AjiI CACGTC 1 cut(s) 250
AluBI AGCT 1 cut(s) 385
AluI AGCT 1 cut(s) 385
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 306
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 190
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
AspS9I GGNCC 2 cut(s) 112, 173
AsuHPI GGTGA 2 cut(s) 16, 49
AsuII TTCGAA 1 cut(s) 328
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 93
BbvI GCAGC 1 cut(s) 177
BceAI ACGGC 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 73
BfrI CTTAAG 1 cut(s) 13
BisI GCNGC 3 cut(s) 191, 335, 338
BlsI GCNGC 3 cut(s) 192, 336, 339
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 1 cut(s) 105
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 112, 173
BmiI GGNNCC 2 cut(s) 46, 175
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 53, 85
Bpu14I TTCGAA 1 cut(s) 328
BsaJI CCNNGG 2 cut(s) 104, 335
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 104, 335
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 1 cut(s) 177
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 1 cut(s) 337
BshFI GGCC 1 cut(s) 174
BshNI GGYRCC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 73
BsmBI CGTCTC 1 cut(s) 73
BsnI GGCC 1 cut(s) 174
BsoBI CYCGRG 1 cut(s) 105
Bsp119I TTCGAA 1 cut(s) 328
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 2 cut(s) 63, 298
BspACI CCGC 2 cut(s) 335, 337
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 1 cut(s) 294
BspFNI CGCG 1 cut(s) 337
BspHI TCATGA 2 cut(s) 33, 204
BspLI GGNNCC 2 cut(s) 46, 175
BspPI GGATC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 328
BspT107I GGYRCC 1 cut(s) 44
BspTI CTTAAG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 104, 335
BssMI GATC 2 cut(s) 63, 298
BstAFI CTTAAG 1 cut(s) 13
BstBI TTCGAA 1 cut(s) 328
BstDEI CTNAG 1 cut(s) 302
BstDSI CCRYGG 1 cut(s) 335
BstFNI CGCG 1 cut(s) 337
BstKTI GATC 2 cut(s) 66, 301
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 2 cut(s) 63, 298
BstMWI GCNNNNNNNGC 3 cut(s) 180, 331, 353
BstSLI GKGCMC 1 cut(s) 185
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 177
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 335
BtrI CACGTC 1 cut(s) 250
CciI TCATGA 2 cut(s) 33, 204
Cfr13I GGNCC 2 cut(s) 112, 173
Cfr42I CCGCGG 1 cut(s) 338
CseI GACGC 1 cut(s) 124
CviAII CATG 5 cut(s) 34, 77, 132, 205, 236
CviJI RGCY 8 cut(s) 21, 174, 325, 334, 340, 385, 391, 403
CviKI_1 RGCY 8 cut(s) 21, 174, 325, 334, 340, 385, 391, 403
DdeI CTNAG 1 cut(s) 302
DpnI GATC 2 cut(s) 65, 300
DpnII GATC 2 cut(s) 63, 298
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 112
Eco88I CYCGRG 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 173
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 5 cut(s) 37, 80, 135, 208, 239
FatI CATG 5 cut(s) 33, 76, 131, 204, 235
FauNDI CATATG 1 cut(s) 319
Fnu4HI GCNGC 3 cut(s) 191, 335, 338
Fsp4HI GCNGC 3 cut(s) 191, 335, 338
GluI GCNGC 3 cut(s) 191, 335, 338
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 1 cut(s) 174
HgaI GACGC 1 cut(s) 124
Hin1II CATG 5 cut(s) 37, 80, 135, 208, 239
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 1 cut(s) 96
HphI GGTGA 2 cut(s) 16, 49
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 2 cut(s) 73, 303
Hpy188III TCNNGA 2 cut(s) 34, 205
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 2 cut(s) 44, 140
HpyAV CCTTC 3 cut(s) 195, 205, 256
HpyCH4IV ACGT 2 cut(s) 30, 249
HpyF10VI GCNNNNNNNGC 3 cut(s) 180, 331, 353
HpyF3I CTNAG 1 cut(s) 302
HpySE526I ACGT 2 cut(s) 30, 249
Hsp92II CATG 5 cut(s) 37, 80, 135, 208, 239
KspI CCGCGG 1 cut(s) 338
Kzo9I GATC 2 cut(s) 63, 298
LmnI GCTCC 1 cut(s) 353
LpnPI CCDG 2 cut(s) 156, 371
Lsp1109I GCAGC 1 cut(s) 177
MaeII ACGT 2 cut(s) 30, 249
MaeIII GTNAC 1 cut(s) 55
MalI GATC 2 cut(s) 65, 300
MboI GATC 2 cut(s) 63, 298
MboII GAAGA 3 cut(s) 98, 193, 265
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 3 cut(s) 196, 268, 308
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 4 cut(s) 11, 114, 238, 393
MseI TTAA 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 337
MspCI CTTAAG 1 cut(s) 13
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 3 cut(s) 180, 331, 353
NdeI CATATG 1 cut(s) 319
NdeII GATC 2 cut(s) 63, 298
NlaIII CATG 5 cut(s) 37, 80, 135, 208, 239
NlaIV GGNNCC 2 cut(s) 46, 175
NmuCI GTSAC 1 cut(s) 55
NspV TTCGAA 1 cut(s) 328
PagI TCATGA 2 cut(s) 33, 204
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 96
PkrI GCNGC 3 cut(s) 192, 336, 339
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PsiI TTATAA 1 cut(s) 152
PspFI CCCAGC 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 46, 175
PspPI GGNCC 2 cut(s) 112, 173
PsuI RGATCY 1 cut(s) 298
SacII CCGCGG 1 cut(s) 338
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 3 cut(s) 191, 335, 338
Sau3AI GATC 2 cut(s) 63, 298
Sau96I GGNCC 2 cut(s) 112, 173
SchI GAGTC 1 cut(s) 105
SduI GDGCHC 1 cut(s) 185
SetI ASST 4 cut(s) 33, 106, 252, 387
Sfr303I CCGCGG 1 cut(s) 338
SfuI TTCGAA 1 cut(s) 328
SgrBI CCGCGG 1 cut(s) 338
SinI GGWCC 1 cut(s) 112
SmlI CTYRAG 2 cut(s) 13, 392
SmoI CTYRAG 2 cut(s) 13, 392
Sse9I AATT 3 cut(s) 196, 268, 308
SsiI CCGC 2 cut(s) 335, 337
TaiI ACGT 2 cut(s) 33, 252
TaqI TCGA 2 cut(s) 99, 328
TasI AATT 3 cut(s) 196, 268, 308
TauI GCSGC 2 cut(s) 337, 340
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 5 cut(s) 17, 21, 193, 224, 224
TspGWI ACGGA 1 cut(s) 98
Vha464I CTTAAG 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.