RchiOBHm_Chr5g0037631

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014275
Physical Location & Seq
Reverse (-)
32265456 .. 32266040
585 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31633

Sequence Viewer

Length: 585 bp
ATGAGCGACAGCACCAAAGTAGTCACTGAGATCGTCTGCGAACACGGTGCAGTGCACCTCTGCCTCTACCTCATCACCCAGAAAATCCGTGACGAGAAGGACAATATGGTTGTAGGGTTTGACAGGGAATGTTCTCTGGTAGACACGGAAGATGGCCGAGTCGAACCTCGGGTGGTCCTTATCAAACTCTTCTCCTGCGTCGGGTGTGTCCTTACAAGATTGAACCATAAGTCAGGCCATGTGTGCCCTTCGCTGCTCAACTTCTTCATGATGAAGGACATTGTTTTTGTTGGAGTTCATGTCAACGAGGACGTGAGGATGTTGAAGGAGAATTATGGGGTTGTGGTTAGGAATGTGGTGGAGCTGAGTGAATTGGCGGCATATGGGCTTCAAAAGCCGCGGCTTTTTGCTTATGGAGCAAGGGATTTGGCAAGAGATGTTTTGAAGCTGGGGCTTGAGCAGAGGCCTCAGAAGGTGGCTTGGATGAGCTGGACTGAGGAGTTTCTGACAGCGGAGCAGATCGAGTGTGTAACAATTGAGGCTTATGTTGCTTATAAAACTGGGAAGAAGTTACTGAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

194

Amino Acids

21.99

Weight (kDa)

6.22

Isoelectric Point (pI)

31.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 33 - 174 2e-08 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11770 AT5G06450
fragaria_vesca FvH4_3g21850
prunus_persica Prupe.4G202600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037631
rosa_laevigata RLG00000033791 RLG00000033797 RLG00000034136
rosa_roxburghii Rroxscaffold_1G00043320 Rroxscaffold_1G00043380
rosa_rugosa Rorug05G0166200
rosa_samantha Rh5BG291800 Rh5CG290500 Rh5DG265600 Rh5DG266100 Rh5DG301600
rosa_wichuraiana Rw5G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 400
AciI CCGC 4 cut(s) 377, 398, 400, 512
AcoI YGGCCR 1 cut(s) 154
AfiI CCNNNNNNNGG 1 cut(s) 201
AgsI TTSAA 4 cut(s) 223, 325, 392, 445
AjiI CACGTC 1 cut(s) 313
AluBI AGCT 4 cut(s) 364, 448, 489, 582
AluI AGCT 4 cut(s) 364, 448, 489, 582
Alw21I GWGCWC 1 cut(s) 57
Alw44I GTGCAC 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 168
AoxI GGCC 3 cut(s) 154, 235, 464
ApaLI GTGCAC 1 cut(s) 53
ApeKI GCWGC 2 cut(s) 253, 579
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AspS9I GGNCC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 1 cut(s) 175
BaeGI GKGCMC 2 cut(s) 57, 248
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 240
BccI CCATC 1 cut(s) 146
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BfaI CTAG 1 cut(s) 583
BisI GCNGC 5 cut(s) 254, 378, 398, 401, 580
BlsI GCNGC 5 cut(s) 255, 379, 399, 402, 581
Bme18I GGWCC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 168
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 175
BmrI ACTGGG 1 cut(s) 570
BmuI ACTGGG 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 476
BsaJI CCNNGG 2 cut(s) 167, 398
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 201
Bse1I ACTGG 1 cut(s) 565
BseDI CCNNGG 2 cut(s) 167, 398
BseGI GGATG 2 cut(s) 324, 489
BseLI CCNNNNNNNGG 1 cut(s) 201
BseMII CTCAG 5 cut(s) 18, 356, 482, 486, 566
BseNI ACTGG 1 cut(s) 565
BseRI GAGGAG 1 cut(s) 512
BseSI GKGCMC 2 cut(s) 57, 248
BseXI GCAGC 1 cut(s) 240
BseYI CCCAGC 1 cut(s) 448
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 400
BshFI GGCC 3 cut(s) 156, 237, 466
BsiHKAI GWGCWC 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 201
BsnI GGCC 3 cut(s) 156, 237, 466
BsoBI CYCGRG 1 cut(s) 168
Bsp1286I GDGCHC 2 cut(s) 57, 248
Bsp143I GATC 2 cut(s) 30, 519
BspACI CCGC 4 cut(s) 377, 398, 400, 512
BspANI GGCC 3 cut(s) 156, 237, 466
BspCNI CTCAG 5 cut(s) 19, 357, 481, 487, 567
BspFNI CGCG 1 cut(s) 400
BspHI TCATGA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 2 cut(s) 167, 398
BssMI GATC 2 cut(s) 30, 519
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 1 cut(s) 194
BstDEI CTNAG 5 cut(s) 27, 365, 468, 495, 575
BstDSI CCRYGG 1 cut(s) 398
BstF5I GGATG 2 cut(s) 324, 489
BstFNI CGCG 1 cut(s) 400
BstKTI GATC 2 cut(s) 33, 522
BstMBI GATC 2 cut(s) 30, 519
BstMWI GCNNNNNNNGC 4 cut(s) 243, 394, 416, 548
BstSLI GKGCMC 2 cut(s) 57, 248
BstUI CGCG 1 cut(s) 400
BstV1I GCAGC 1 cut(s) 240
BsuRI GGCC 3 cut(s) 156, 237, 466
BtgI CCRYGG 1 cut(s) 398
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 2 cut(s) 324, 489
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 2 cut(s) 24, 57
CciI TCATGA 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 175
Cfr42I CCGCGG 1 cut(s) 401
CseI GACGC 1 cut(s) 187
CviAII CATG 3 cut(s) 239, 268, 299
DdeI CTNAG 5 cut(s) 27, 365, 468, 495, 575
DpnI GATC 2 cut(s) 32, 521
DpnII GATC 2 cut(s) 30, 519
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco147I AGGCCT 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 168
FaeI CATG 3 cut(s) 242, 271, 302
FatI CATG 3 cut(s) 238, 267, 298
FauNDI CATATG 1 cut(s) 382
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 5 cut(s) 254, 378, 398, 401, 580
FokI GGATG 2 cut(s) 331, 496
Fsp4HI GCNGC 5 cut(s) 254, 378, 398, 401, 580
FspBI CTAG 1 cut(s) 583
GluI GCNGC 5 cut(s) 254, 378, 398, 401, 580
GsaI CCCAGC 1 cut(s) 452
HaeIII GGCC 3 cut(s) 156, 237, 466
HgaI GACGC 1 cut(s) 187
Hin1II CATG 3 cut(s) 242, 271, 302
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 1 cut(s) 159
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 3 cut(s) 55, 142, 304
Hpy188I TCNGA 2 cut(s) 471, 507
Hpy188III TCNNGA 1 cut(s) 268
Hpy8I GTNNAC 3 cut(s) 55, 142, 304
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 5 cut(s) 91, 258, 268, 319, 466
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 2 cut(s) 50, 55
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 394, 416, 548
HpyF3I CTNAG 5 cut(s) 27, 365, 468, 495, 575
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 3 cut(s) 242, 271, 302
KspI CCGCGG 1 cut(s) 401
Kzo9I GATC 2 cut(s) 30, 519
LmnI GCTCC 3 cut(s) 361, 416, 514
LpnPI CCDG 8 cut(s) 92, 109, 122, 208, 219, 434, 475, 546
Lsp1109I GCAGC 1 cut(s) 240
MaeI CTAG 1 cut(s) 583
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 4 cut(s) 22, 89, 529, 570
MalI GATC 2 cut(s) 32, 521
MboI GATC 2 cut(s) 30, 519
MboII GAAGA 4 cut(s) 161, 181, 256, 577
MfeI CAATTG 1 cut(s) 534
MhlI GDGCHC 2 cut(s) 57, 248
MluCI AATT 3 cut(s) 331, 371, 534
MlyI GAGTC 1 cut(s) 168
MmeI TCCRAC 1 cut(s) 271
MspA1I CMGCKG 2 cut(s) 400, 512
MunI CAATTG 1 cut(s) 534
MvnI CGCG 1 cut(s) 400
MwoI GCNNNNNNNGC 4 cut(s) 243, 394, 416, 548
NdeI CATATG 1 cut(s) 382
NdeII GATC 2 cut(s) 30, 519
NlaIII CATG 3 cut(s) 242, 271, 302
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 2 cut(s) 22, 89
PagI TCATGA 1 cut(s) 267
PceI AGGCCT 1 cut(s) 466
PkrI GCNGC 5 cut(s) 255, 379, 399, 402, 581
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PsiI TTATAA 1 cut(s) 555
PspFI CCCAGC 1 cut(s) 448
PspPI GGNCC 1 cut(s) 175
SacII CCGCGG 1 cut(s) 401
SatI GCNGC 5 cut(s) 254, 378, 398, 401, 580
Sau3AI GATC 2 cut(s) 30, 519
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 1 cut(s) 168
SduI GDGCHC 2 cut(s) 57, 248
SetI ASST 9 cut(s) 60, 72, 169, 315, 366, 450, 477, 491, 584
Sfr303I CCGCGG 1 cut(s) 401
SgrBI CCGCGG 1 cut(s) 401
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 3 cut(s) 331, 371, 534
SseBI AGGCCT 1 cut(s) 466
SsiI CCGC 4 cut(s) 377, 398, 400, 512
SspMI CTAG 1 cut(s) 583
StuI AGGCCT 1 cut(s) 466
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 162, 522
TasI AATT 3 cut(s) 331, 371, 534
TauI GCSGC 3 cut(s) 380, 400, 403
TscAI CASTG 2 cut(s) 31, 57
TseFI GTSAC 2 cut(s) 22, 89
TseI GCWGC 2 cut(s) 253, 579
Tsp45I GTSAC 2 cut(s) 22, 89
TspDTI ATGAA 3 cut(s) 256, 287, 287
TspGWI ACGGA 2 cut(s) 77, 161
TspRI CASTG 2 cut(s) 31, 57
VneI GTGCAC 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 175
XmiI GTMKAC 1 cut(s) 141
XspI CTAG 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.