FvH4_3g23200

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015575
Physical Location & Seq
Forward (+)
16278255 .. 16280553
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23200.t1

Sequence Viewer

Length: 1260 bp
ATGTGGTATGCATTACTTCTCATACTGATCGTCTCCTCCTCCTTTGTCCCCACCACTGCTCATAGAACCAATGCTTGCACTCCCATCAACCCCATGAACATCACCAGCTGCAAGAAACTAATCACCTTGGGAGCTGAACTCGGCTGGAACATCCACAACGACAACAAAACCCACATTGACATCTTGTTCACCATTCCAATATCTACAAACGACGCACCAAAATGGGTCGCCTGGGGTGTGCACCCAGGTCGGAAAAGGCCTCAGATGATCGGAACCAGGGCCTTTATCGCCATTTCACAGTCCAACGGGACTTTGAAGGTCAGCAAATACAACATCACTAGTGACACCAAGCTGGGATGTAGTTTGCAGCCTCTTCAGCCTTCCGAAGAATTCGAGGATGTCGTCGTCCAAAACATGACAGGTGAAGTGAAACCTAACAGATACATGTCCATTTTTGCGACGCTTATTTTGCGTCCAGATTTAGCTCCGTACGACATTGAAAGGTTGAACCATGTGTGGCAAGTTGGGTATGAAGCTGATGAGGTTCATCTGGAGCCTAAGATGCACCCGACGGCTCTCCAGAACGTGGATAGCACAGAGACTACAAACTTGTTCAATCGGAAAGGTATCAGCATTGGACATCGCCGGCATCATCTTAGAACGGTGCATGGGATTCTTAATATGTTGGGATGGGGAACATTGTTGCCGTTTGGAGTGATTATAGCAAGGTACTACAGAAGATATCCCTTTCATTGTAAAAAGTGGTATTTCTGTCACGTCTCCTGTCAGATCGTTGGTTATATTCTCGGCACGGCTGGTTGGGCAATCGGAATATGGCTCGGACATGCTTCCAAAGAATATCACTTCCACACTCACAATATTCTCGCCATGTTCATATTTCCATTCACCACATTACAAATGATTGCTCTGCGTTTACGGCCCATGCCGACCGATGATTACCGGAAATACTGGGACATGTACCATCATTTTCTGGGATATGCACTTCTGGCTGTGATCATAGTGAACATATTCCACGGCATTGCCATTTTGAAGCCAGAAAAAACCTGGCTATGGGCTTATGTTGGAATCCTTGCGTCGCTTGCTTCCATTGCATTGTGTTCGGAGATTTATACTTGGTGTAAGTTCATGAAATCCAAAATGAAAAAAGCAGCAGCAAACGGCCAAACTGGAGATGCTCAATCTACTACTCAACCCACTACCACAAAAACTTTATCAGACAATGCGCACACTACAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

420

Amino Acids

47.64

Weight (kDa)

9.3

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 89 - 204 1.9e-19 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015575)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23200
malus_domestica MD03G1196700.v1.1 MD11G1212800.v1.1
prunus_persica Prupe.4G218700_v2.0.a1
pyrus_communis pycom11g18780
rosa_laevigata RLG00000034009
rosa_multiflora Rmu_sc0004953.1_g000005 Rmu_sc0004953.1_g000008
rosa_roxburghii Rroxscaffold_1G00039840
rosa_rugosa Rorug05G0188200 Rorug05G0220100
rosa_samantha Rh5AG273700 Rh5BG278800 Rh5CG310800 Rh5DG287000
rosa_wichuraiana Rw5G025730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1245
AccB7I CCANNNNNTGG 1 cut(s) 586
AcoI YGGCCR 1 cut(s) 1180
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 359
AfaI GTAC 3 cut(s) 491, 731, 980
AfiI CCNNNNNNNGG 2 cut(s) 586, 1071
AflIII ACRYGT 2 cut(s) 444, 975
AgsI TTSAA 5 cut(s) 316, 500, 508, 616, 1051
AhlI ACTAGT 1 cut(s) 338
AjiI CACGTC 1 cut(s) 778
AjnI CCWGG 4 cut(s) 230, 244, 275, 1064
AluBI AGCT 5 cut(s) 108, 134, 352, 485, 536
AluI AGCT 5 cut(s) 108, 134, 352, 485, 536
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 3 cut(s) 37, 593, 784
Alw44I GTGCAC 1 cut(s) 239
AoxI GGCC 4 cut(s) 257, 279, 938, 1180
ApaLI GTGCAC 1 cut(s) 239
ApeKI GCWGC 4 cut(s) 108, 367, 1169, 1172
ApoI RAATTY 1 cut(s) 389
ArsI GACNNNNNNTTYG 2 cut(s) 451, 483
Asp700I GAANNNNTTC 1 cut(s) 1028
AspLEI GCGC 1 cut(s) 1246
AspS9I GGNCC 2 cut(s) 279, 939
AsuHPI GGTGA 5 cut(s) 94, 115, 181, 434, 898
BaeGI GKGCMC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 4 cut(s) 95, 379, 1181, 1184
BccI CCATC 3 cut(s) 92, 684, 990
BceAI ACGGC 6 cut(s) 588, 691, 828, 953, 1051, 1195
BcgI CGANNNNNNTGC 4 cut(s) 230, 264, 1101, 1135
BciT130I CCWGG 4 cut(s) 232, 246, 277, 1066
BclI TGATCA 1 cut(s) 1014
BcoDI GTCTC 3 cut(s) 37, 593, 784
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 1 cut(s) 339
BfmI CTRYAG 1 cut(s) 733
BisI GCNGC 4 cut(s) 109, 368, 1170, 1173
BlsI GCNGC 4 cut(s) 110, 369, 1171, 1174
Bme1390I CCNGG 4 cut(s) 232, 246, 277, 1066
BmgBI CACGTC 1 cut(s) 778
BmgT120I GGNCC 2 cut(s) 279, 939
BmiI GGNNCC 2 cut(s) 274, 555
BmrFI CCNGG 4 cut(s) 232, 246, 277, 1066
BmrI ACTGGG 1 cut(s) 979
BmsI GCATC 3 cut(s) 552, 658, 1183
BmuI ACTGGG 1 cut(s) 979
BplI GAGNNNNNCTC 2 cut(s) 123, 155
BpmI CTGGAG 3 cut(s) 563, 572, 1209
BsaJI CCNNGG 5 cut(s) 126, 231, 244, 276, 1033
BsaWI WCCGGW 1 cut(s) 960
Bsc4I CCNNNNNNNGG 2 cut(s) 586, 1071
Bse118I RCCGGY 1 cut(s) 645
Bse1I ACTGG 2 cut(s) 974, 1192
Bse3DI GCAATG 2 cut(s) 1038, 1107
BseBI CCWGG 4 cut(s) 232, 246, 277, 1066
BseDI CCNNGG 5 cut(s) 126, 231, 244, 276, 1033
BseGI GGATG 4 cut(s) 150, 362, 403, 695
BseLI CCNNNNNNNGG 2 cut(s) 586, 1071
BseMI GCAATG 2 cut(s) 1038, 1107
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 2 cut(s) 974, 1192
BseRI GAGGAG 2 cut(s) 25, 28
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 4 cut(s) 95, 379, 1181, 1184
BseYI CCCAGC 1 cut(s) 352
Bsh1285I CGRYCG 1 cut(s) 951
BshFI GGCC 4 cut(s) 259, 281, 940, 1182
BsiEI CGRYCG 1 cut(s) 951
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 2 cut(s) 646, 961
BsiWI CGTACG 1 cut(s) 489
BslFI GGGAC 3 cut(s) 32, 322, 986
BslI CCNNNNNNNGG 2 cut(s) 586, 1071
BsmAI GTCTC 3 cut(s) 37, 593, 784
BsmBI CGTCTC 2 cut(s) 37, 784
BsmFI GGGAC 3 cut(s) 32, 322, 986
BsnI GGCC 4 cut(s) 259, 281, 940, 1182
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 4 cut(s) 27, 267, 789, 1014
BspANI GGCC 4 cut(s) 259, 281, 940, 1182
BspCNI CTCAG 1 cut(s) 274
BspHI TCATGA 1 cut(s) 1146
BspLI GGNNCC 2 cut(s) 274, 555
BsrDI GCAATG 2 cut(s) 1038, 1107
BsrFI RCCGGY 1 cut(s) 645
BsrI ACTGG 2 cut(s) 974, 1192
BssAI RCCGGY 1 cut(s) 645
BssECI CCNNGG 5 cut(s) 126, 231, 244, 276, 1033
BssMI GATC 4 cut(s) 27, 267, 789, 1014
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 4 cut(s) 232, 246, 277, 1066
Bst4CI ACNGT 2 cut(s) 300, 664
Bst6I CTCTTC 1 cut(s) 378
BstC8I GCNNGC 3 cut(s) 76, 647, 1101
BstDEI CTNAG 3 cut(s) 261, 558, 656
BstDSI CCRYGG 1 cut(s) 1033
BstF5I GGATG 4 cut(s) 150, 362, 403, 695
BstHHI GCGC 1 cut(s) 1246
BstKTI GATC 4 cut(s) 30, 270, 792, 1017
BstMAI GTCTC 3 cut(s) 37, 593, 784
BstMBI GATC 4 cut(s) 27, 267, 789, 1014
BstMCI CGRYCG 1 cut(s) 951
BstMWI GCNNNNNNNGC 9 cut(s) 287, 376, 469, 562, 821, 937, 1007, 1100, 1109
BstNI CCWGG 4 cut(s) 232, 246, 277, 1066
BstNSI RCATGY 3 cut(s) 448, 848, 979
BstSCI CCNGG 4 cut(s) 230, 244, 275, 1064
BstSFI CTRYAG 1 cut(s) 733
BstSLI GKGCMC 1 cut(s) 243
BstV1I GCAGC 4 cut(s) 95, 379, 1181, 1184
BsuRI GGCC 4 cut(s) 259, 281, 940, 1182
BtgI CCRYGG 1 cut(s) 1033
BtgZI GCGATG 1 cut(s) 626
BtrI CACGTC 1 cut(s) 778
BtsCI GGATG 4 cut(s) 150, 362, 403, 695
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 3 cut(s) 76, 647, 1101
CciI TCATGA 1 cut(s) 1146
CfoI GCGC 1 cut(s) 1246
Cfr10I RCCGGY 1 cut(s) 645
Cfr13I GGNCC 2 cut(s) 279, 939
CseI GACGC 4 cut(s) 221, 461, 469, 1083
Csp6I GTAC 3 cut(s) 490, 730, 979
CviQI GTAC 3 cut(s) 490, 730, 979
DdeI CTNAG 3 cut(s) 261, 558, 656
DpnI GATC 4 cut(s) 29, 269, 791, 1016
DpnII GATC 4 cut(s) 27, 267, 789, 1014
EaeI YGGCCR 1 cut(s) 1180
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 126
Eco147I AGGCCT 1 cut(s) 259
Eco32I GATATC 1 cut(s) 743
Eco57I CTGAAG 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 279
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 4 cut(s) 230, 244, 275, 1064
EcoRV GATATC 1 cut(s) 743
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 126
Esp3I CGTCTC 2 cut(s) 37, 784
FaqI GGGAC 3 cut(s) 32, 322, 986
FbaI TGATCA 1 cut(s) 1014
Fnu4HI GCNGC 4 cut(s) 109, 368, 1170, 1173
FokI GGATG 4 cut(s) 137, 369, 410, 702
Fsp4HI GCNGC 4 cut(s) 109, 368, 1170, 1173
FspAI RTGCGCAY 1 cut(s) 1245
FspBI CTAG 1 cut(s) 339
FspI TGCGCA 1 cut(s) 1245
GlaI GCGC 1 cut(s) 1245
GluI GCNGC 4 cut(s) 109, 368, 1170, 1173
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 3 cut(s) 563, 572, 1209
HaeIII GGCC 4 cut(s) 259, 281, 940, 1182
HapII CCGG 2 cut(s) 646, 961
HgaI GACGC 4 cut(s) 221, 461, 469, 1083
HhaI GCGC 1 cut(s) 1246
Hin6I GCGC 1 cut(s) 1244
HinP1I GCGC 1 cut(s) 1244
HinfI GANTC 2 cut(s) 673, 1086
HpaII CCGG 2 cut(s) 646, 961
HphI GGTGA 5 cut(s) 94, 115, 181, 434, 898
Hpy166II GTNNAC 4 cut(s) 189, 241, 935, 1024
Hpy188III TCNNGA 4 cut(s) 476, 551, 580, 1147
Hpy8I GTNNAC 4 cut(s) 189, 241, 935, 1024
Hpy99I CGWCG 5 cut(s) 215, 407, 463, 574, 1099
HpyAV CCTTC 2 cut(s) 310, 390
HpyCH4III ACNGT 2 cut(s) 300, 664
HpyCH4IV ACGT 2 cut(s) 585, 777
HpyCH4V TGCA 9 cut(s) 11, 78, 111, 241, 367, 565, 667, 1001, 1112
HpyF10VI GCNNNNNNNGC 9 cut(s) 287, 376, 469, 562, 821, 937, 1007, 1100, 1109
HpyF3I CTNAG 3 cut(s) 261, 558, 656
HpySE526I ACGT 2 cut(s) 585, 777
HspAI GCGC 1 cut(s) 1244
KroI GCCGGC 1 cut(s) 645
KroNI GCCGGC 1 cut(s) 647
Ksp22I TGATCA 1 cut(s) 1014
Kzo9I GATC 4 cut(s) 27, 267, 789, 1014
LmnI GCTCC 3 cut(s) 131, 490, 553
Lsp1109I GCAGC 4 cut(s) 95, 379, 1181, 1184
LweI GCATC 3 cut(s) 552, 658, 1183
MaeI CTAG 1 cut(s) 339
MaeII ACGT 2 cut(s) 585, 777
MaeIII GTNAC 2 cut(s) 341, 773
MalI GATC 4 cut(s) 29, 269, 791, 1016
MboI GATC 4 cut(s) 27, 267, 789, 1014
MboII GAAGA 3 cut(s) 365, 398, 750
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 1 cut(s) 389
MmeI TCCRAC 3 cut(s) 230, 327, 1063
MnlI CCTC 6 cut(s) 46, 49, 270, 381, 388, 535
Mph1103I ATGCAT 1 cut(s) 13
MroNI GCCGGC 1 cut(s) 645
MroXI GAANNNNTTC 1 cut(s) 1028
MseI TTAA 1 cut(s) 678
MslI CAYNNNNRTG 1 cut(s) 220
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 2 cut(s) 646, 961
MspR9I CCNGG 4 cut(s) 232, 246, 277, 1066
MvaI CCWGG 4 cut(s) 232, 246, 277, 1066
MwoI GCNNNNNNNGC 9 cut(s) 287, 376, 469, 562, 821, 937, 1007, 1100, 1109
NaeI GCCGGC 1 cut(s) 647
NdeII GATC 4 cut(s) 27, 267, 789, 1014
NgoMIV GCCGGC 1 cut(s) 645
NlaIV GGNNCC 2 cut(s) 274, 555
NmeAIII GCCGAG 2 cut(s) 120, 786
NmuCI GTSAC 2 cut(s) 341, 773
NsbI TGCGCA 1 cut(s) 1245
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 3 cut(s) 448, 848, 979
PagI TCATGA 1 cut(s) 1146
PceI AGGCCT 1 cut(s) 259
PciI ACATGT 2 cut(s) 444, 975
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PdiI GCCGGC 1 cut(s) 647
PdmI GAANNNNTTC 1 cut(s) 1028
PfeI GAWTC 2 cut(s) 673, 1086
Pfl23II CGTACG 1 cut(s) 489
PflMI CCANNNNNTGG 1 cut(s) 586
PkrI GCNGC 4 cut(s) 110, 369, 1171, 1174
PscI ACATGT 2 cut(s) 444, 975
Psp6I CCWGG 4 cut(s) 230, 244, 275, 1064
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 4 cut(s) 230, 244, 275, 1064
PspLI CGTACG 1 cut(s) 489
PspN4I GGNNCC 2 cut(s) 274, 555
PspPI GGNCC 2 cut(s) 279, 939
PvuII CAGCTG 1 cut(s) 108
RsaI GTAC 3 cut(s) 491, 731, 980
RsaNI GTAC 3 cut(s) 490, 730, 979
RseI CAYNNNNRTG 1 cut(s) 220
SaqAI TTAA 1 cut(s) 678
SatI GCNGC 4 cut(s) 109, 368, 1170, 1173
Sau3AI GATC 4 cut(s) 27, 267, 789, 1014
Sau96I GGNCC 2 cut(s) 279, 939
ScrFI CCNGG 4 cut(s) 232, 246, 277, 1066
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 3 cut(s) 552, 658, 1183
SfcI CTRYAG 1 cut(s) 733
SmiMI CAYNNNNRTG 1 cut(s) 220
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 1 cut(s) 389
SseBI AGGCCT 1 cut(s) 259
SspI AATATT 1 cut(s) 880
SspMI CTAG 1 cut(s) 339
StuI AGGCCT 1 cut(s) 259
StyD4I CCNGG 4 cut(s) 230, 244, 275, 1064
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 300, 664
TaiI ACGT 2 cut(s) 588, 780
TaqI TCGA 1 cut(s) 393
TaqII GACCGA 1 cut(s) 965
TasI AATT 1 cut(s) 389
TfiI GAWTC 2 cut(s) 673, 1086
Tru1I TTAA 1 cut(s) 678
Tru9I TTAA 1 cut(s) 678
TscAI CASTG 1 cut(s) 61
TseFI GTSAC 2 cut(s) 341, 773
TseI GCWGC 4 cut(s) 108, 367, 1169, 1172
Tsp45I GTSAC 2 cut(s) 341, 773
TspDTI ATGAA 8 cut(s) 110, 536, 546, 740, 883, 1135, 1163, 1175
TspGWI ACGGA 1 cut(s) 477
TspRI CASTG 1 cut(s) 61
Van91I CCANNNNNTGG 1 cut(s) 586
VneI GTGCAC 1 cut(s) 239
XapI RAATTY 1 cut(s) 389
XceI RCATGY 3 cut(s) 448, 848, 979
XcmI CCANNNNNNNNNTGG 1 cut(s) 1062
XmnI GAANNNNTTC 1 cut(s) 1028
XspI CTAG 1 cut(s) 339
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.