RLG00000034009

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015575
Physical Location & Seq
Forward (+)
35276191 .. 35278294
2104 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034009

Sequence Viewer

Length: 1218 bp
ATGTACTGGTGTGCATTAATTCTCGTACTGACCTTGTCCTCCTCCTCCTCTGTCGCCACCACTGCCCATAAGAGAACCAATATTTGTACTCCCATCAACCCCATGAACATCACCAACTGCAAGAAACTACTCACTTTGGGAGCTGAACTCGGCTGGAACATCCACAACAAAACCCACATTGACATCTTGTTCACCATTCCAATATCTATGGACGACACACCAAAATGGGTCGCCTGGGGTGTGAACCCGCTTGGGAAAAGGCCGCGGATGATCGGAACCAGGGCCATTATCGCCATTTCACAGGCCAACGGGACTTTGAAGGTCAGCAGATACAACATCACTAGTGACACCAAGCTGGGATGTCGTTTGCAGCCTCTGCAGCCTTCCATAGAGTTCGAGGATGTCGTCGTCCAAAACATGACAGGTTATGTGAGACCTAACTGTTACATGTCAATTTTTGCGACGCTAATTTTGCGTCCAGATTTAGTTGCGTACGACATTGCAAGGTTGAACCATGTGTGGCAAGTTGGGTATGAAGCTGATGAGGTTGCTCTGGAGCCTAAGATGCACCCGACGGCTCTCCAGAACGTGGATAGCACAGAGACCACAAACTTGTACAGTTGGAAAGGCATCAGCATTGGACATCGCCGGCGTCGTCTTAGAACGGTGCATGGGATTCTGAATATTGTGGGATGGGGAACATTGTTGCCCTTTGGAGTGATTATAGCGAGGTACTGCAGAAGATATCCCTTTGCTAGTACAAATTGGTTTTTATCACACGTCTCTTGTCAGATCGTTGGTTACATTCTCGGCACAATTGGTTGGGCAATCGGAATATGGCTCGGACATGCTTCCAAAGAATATCACTTCCACACTCACAATGTTCTCGCCATGTTCATATTTGCATTTACAACATTACAAATGATTGCTCTGCGTTTAAGGCCAACGCCAACAGATGATTACCGGAAATACTGGGACATGTACCATCATTTTCTGGGCTACGCACTTCTGGCTGTGATCATATTGAATATATTCCATGGCATTGCCATTTTGAAGCCAGACAAAACCTGGCTATGGGCTTATATTGGAATCCTTGCAGTGCTTGCTTCCATCACATTGGGTTTGGAGATTTATACTTGGTGTAAATTCATGAAATCCAAAATGAAGAAACCAACTGCCGCAGAAGTTCAACCTAGTAGTCAGTCTCATACCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

406

Amino Acids

45.95

Weight (kDa)

9.51

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 90 - 205 2.3e-18 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015575)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23200
malus_domestica MD03G1196700.v1.1 MD11G1212800.v1.1
prunus_persica Prupe.4G218700_v2.0.a1
pyrus_communis pycom11g18780
rosa_laevigata RLG00000034009
rosa_multiflora Rmu_sc0004953.1_g000005 Rmu_sc0004953.1_g000008
rosa_roxburghii Rroxscaffold_1G00039840
rosa_rugosa Rorug05G0188200 Rorug05G0220100
rosa_samantha Rh5AG273700 Rh5BG278800 Rh5CG310800 Rh5DG287000
rosa_wichuraiana Rw5G025730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 589
AccII CGCG 1 cut(s) 265
AciI CCGC 4 cut(s) 248, 263, 265, 1179
AcsI RAATTY 1 cut(s) 1145
AcyI GRCGYC 1 cut(s) 652
AfaI GTAC 8 cut(s) 5, 27, 88, 494, 617, 734, 760, 983
AfiI CCNNNNNNNGG 2 cut(s) 589, 1074
AflIII ACRYGT 3 cut(s) 447, 778, 978
AgsI TTSAA 5 cut(s) 319, 511, 1027, 1054, 1190
AhlI ACTAGT 1 cut(s) 341
AjiI CACGTC 1 cut(s) 781
AjnI CCWGG 3 cut(s) 233, 278, 1067
AluBI AGCT 3 cut(s) 143, 355, 539
AluI AGCT 3 cut(s) 143, 355, 539
Alw26I GTCTC 4 cut(s) 427, 596, 787, 1209
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 4 cut(s) 260, 282, 303, 941
ApeKI GCWGC 2 cut(s) 370, 379
ApoI RAATTY 1 cut(s) 1145
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
AseI ATTAAT 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 1031
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 103, 184
BbvI GCAGC 2 cut(s) 382, 391
BccI CCATC 4 cut(s) 101, 687, 993, 1118
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 3 cut(s) 235, 280, 1069
BclI TGATCA 1 cut(s) 1017
BcoDI GTCTC 4 cut(s) 427, 596, 787, 1209
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 4 cut(s) 342, 756, 1194, 1216
BfmI CTRYAG 2 cut(s) 377, 736
BisI GCNGC 4 cut(s) 263, 371, 380, 1179
BlsI GCNGC 4 cut(s) 264, 372, 381, 1180
Bme1390I CCNGG 3 cut(s) 235, 280, 1069
BmgBI CACGTC 1 cut(s) 781
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 277, 558
BmrFI CCNGG 3 cut(s) 235, 280, 1069
BmrI ACTGGG 1 cut(s) 982
BmsI GCATC 2 cut(s) 555, 639
BmuI ACTGGG 1 cut(s) 982
BplI GAGNNNNNCTC 2 cut(s) 132, 164
BpmI CTGGAG 2 cut(s) 566, 575
BsaHI GRCGYC 1 cut(s) 652
BsaI GGTCTC 2 cut(s) 427, 596
BsaJI CCNNGG 4 cut(s) 234, 263, 279, 1036
BsaWI WCCGGW 1 cut(s) 963
Bsc4I CCNNNNNNNGG 2 cut(s) 589, 1074
Bse118I RCCGGY 1 cut(s) 648
Bse1I ACTGG 2 cut(s) 11, 977
Bse3DI GCAATG 2 cut(s) 498, 1041
BseBI CCWGG 3 cut(s) 235, 280, 1069
BseDI CCNNGG 4 cut(s) 234, 263, 279, 1036
BseGI GGATG 5 cut(s) 159, 273, 365, 406, 698
BseLI CCNNNNNNNGG 2 cut(s) 589, 1074
BseMI GCAATG 2 cut(s) 498, 1041
BseNI ACTGG 2 cut(s) 11, 977
BseRI GAGGAG 3 cut(s) 31, 34, 37
BseXI GCAGC 2 cut(s) 382, 391
BseYI CCCAGC 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 265
BshFI GGCC 4 cut(s) 262, 284, 305, 943
BsiSI CCGG 2 cut(s) 649, 964
BsiWI CGTACG 1 cut(s) 492
BslFI GGGAC 2 cut(s) 325, 989
BslI CCNNNNNNNGG 2 cut(s) 589, 1074
BsmAI GTCTC 4 cut(s) 427, 596, 787, 1209
BsmBI CGTCTC 1 cut(s) 787
BsmFI GGGAC 2 cut(s) 325, 989
BsnI GGCC 4 cut(s) 262, 284, 305, 943
Bso31I GGTCTC 2 cut(s) 427, 596
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 3 cut(s) 270, 792, 1017
Bsp19I CCATGG 1 cut(s) 1036
BspACI CCGC 4 cut(s) 248, 263, 265, 1179
BspANI GGCC 4 cut(s) 262, 284, 305, 943
BspFNI CGCG 1 cut(s) 265
BspHI TCATGA 1 cut(s) 1149
BspLI GGNNCC 2 cut(s) 277, 558
BspMAI CTGCAG 2 cut(s) 381, 740
BspTNI GGTCTC 2 cut(s) 427, 596
BsrDI GCAATG 2 cut(s) 498, 1041
BsrFI RCCGGY 1 cut(s) 648
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 2 cut(s) 11, 977
BssAI RCCGGY 1 cut(s) 648
BssECI CCNNGG 4 cut(s) 234, 263, 279, 1036
BssMI GATC 3 cut(s) 270, 792, 1017
BssNI GRCGYC 1 cut(s) 652
BssT1I CCWWGG 1 cut(s) 1036
Bst2UI CCWGG 3 cut(s) 235, 280, 1069
Bst4CI ACNGT 3 cut(s) 443, 620, 667
BstACI GRCGYC 1 cut(s) 652
BstAPI GCANNNNNTGC 2 cut(s) 376, 1103
BstAUI TGTACA 1 cut(s) 615
BstC8I GCNNGC 2 cut(s) 650, 1104
BstDEI CTNAG 2 cut(s) 561, 659
BstDSI CCRYGG 2 cut(s) 263, 1036
BstF5I GGATG 5 cut(s) 159, 273, 365, 406, 698
BstFNI CGCG 1 cut(s) 265
BstKTI GATC 3 cut(s) 273, 795, 1020
BstMAI GTCTC 4 cut(s) 427, 596, 787, 1209
BstMBI GATC 3 cut(s) 270, 792, 1017
BstMWI GCNNNNNNNGC 9 cut(s) 62, 290, 376, 379, 472, 565, 940, 1010, 1103
BstNI CCWGG 3 cut(s) 235, 280, 1069
BstNSI RCATGY 3 cut(s) 451, 851, 982
BstSCI CCNGG 3 cut(s) 233, 278, 1067
BstSFI CTRYAG 2 cut(s) 377, 736
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 2 cut(s) 382, 391
BstXI CCANNNNNNTGG 1 cut(s) 1117
BsuRI GGCC 4 cut(s) 262, 284, 305, 943
BtgI CCRYGG 2 cut(s) 263, 1036
BtgZI GCGATG 1 cut(s) 629
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 5 cut(s) 159, 273, 365, 406, 698
BtsI GCAGTG 2 cut(s) 60, 1104
BtsIMutI CAGTG 2 cut(s) 60, 1104
Cac8I GCNNGC 2 cut(s) 650, 1104
CaiI CAGNNNCTG 1 cut(s) 376
CciI TCATGA 1 cut(s) 1149
Cfr10I RCCGGY 1 cut(s) 648
Cfr13I GGNCC 1 cut(s) 282
Cfr42I CCGCGG 1 cut(s) 266
CseI GACGC 3 cut(s) 464, 472, 641
Csp6I GTAC 8 cut(s) 4, 26, 87, 493, 616, 733, 759, 982
CviQI GTAC 8 cut(s) 4, 26, 87, 493, 616, 733, 759, 982
DdeI CTNAG 2 cut(s) 561, 659
DpnI GATC 3 cut(s) 272, 794, 1019
DpnII GATC 3 cut(s) 270, 792, 1017
Eco130I CCWWGG 1 cut(s) 1036
Eco31I GGTCTC 2 cut(s) 427, 596
Eco32I GATATC 1 cut(s) 746
EcoRII CCWGG 3 cut(s) 233, 278, 1067
EcoRV GATATC 1 cut(s) 746
EcoT14I CCWWGG 1 cut(s) 1036
ErhI CCWWGG 1 cut(s) 1036
Esp3I CGTCTC 1 cut(s) 787
FaqI GGGAC 2 cut(s) 325, 989
FauI CCCGC 1 cut(s) 255
FbaI TGATCA 1 cut(s) 1017
Fnu4HI GCNGC 4 cut(s) 263, 371, 380, 1179
FokI GGATG 5 cut(s) 146, 280, 372, 413, 705
Fsp4HI GCNGC 4 cut(s) 263, 371, 380, 1179
FspBI CTAG 4 cut(s) 342, 756, 1194, 1216
GluI GCNGC 4 cut(s) 263, 371, 380, 1179
GsaI CCCAGC 1 cut(s) 359
GsuI CTGGAG 2 cut(s) 566, 575
HaeIII GGCC 4 cut(s) 262, 284, 305, 943
HapII CCGG 2 cut(s) 649, 964
HgaI GACGC 3 cut(s) 464, 472, 641
Hin1I GRCGYC 1 cut(s) 652
HinfI GANTC 2 cut(s) 676, 1089
HpaII CCGG 2 cut(s) 649, 964
HphI GGTGA 2 cut(s) 103, 184
Hpy166II GTNNAC 2 cut(s) 192, 244
Hpy188I TCNGA 5 cut(s) 275, 681, 792, 833, 845
Hpy188III TCNNGA 4 cut(s) 479, 554, 583, 1150
Hpy8I GTNNAC 2 cut(s) 192, 244
Hpy99I CGWCG 4 cut(s) 410, 466, 577, 657
HpyAV CCTTC 2 cut(s) 313, 393
HpyCH4III ACNGT 3 cut(s) 443, 620, 667
HpyCH4IV ACGT 2 cut(s) 588, 780
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 290, 376, 379, 472, 565, 940, 1010, 1103
HpyF3I CTNAG 2 cut(s) 561, 659
HpySE526I ACGT 2 cut(s) 588, 780
Hsp92I GRCGYC 1 cut(s) 652
KroI GCCGGC 1 cut(s) 648
KroNI GCCGGC 1 cut(s) 650
Ksp22I TGATCA 1 cut(s) 1017
KspI CCGCGG 1 cut(s) 266
Kzo9I GATC 3 cut(s) 270, 792, 1017
LmnI GCTCC 2 cut(s) 140, 556
Lsp1109I GCAGC 2 cut(s) 382, 391
LweI GCATC 2 cut(s) 555, 639
MaeI CTAG 4 cut(s) 342, 756, 1194, 1216
MaeII ACGT 2 cut(s) 588, 780
MaeIII GTNAC 3 cut(s) 344, 443, 800
MalI GATC 3 cut(s) 272, 794, 1019
MboI GATC 3 cut(s) 270, 792, 1017
MboII GAAGA 2 cut(s) 753, 1177
MfeI CAATTG 1 cut(s) 816
MluCI AATT 6 cut(s) 18, 453, 468, 763, 816, 1145
MmeI TCCRAC 1 cut(s) 602
MnlI CCTC 8 cut(s) 49, 52, 55, 58, 384, 391, 538, 723
MreI CGCCGGCG 1 cut(s) 648
MroNI GCCGGC 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 1031
MseI TTAA 2 cut(s) 17, 938
MslI CAYNNNNRTG 1 cut(s) 223
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 2 cut(s) 649, 964
MspR9I CCNGG 3 cut(s) 235, 280, 1069
MunI CAATTG 1 cut(s) 816
MvaI CCWGG 3 cut(s) 235, 280, 1069
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 9 cut(s) 62, 290, 376, 379, 472, 565, 940, 1010, 1103
NaeI GCCGGC 1 cut(s) 650
NcoI CCATGG 1 cut(s) 1036
NdeII GATC 3 cut(s) 270, 792, 1017
NgoMIV GCCGGC 1 cut(s) 648
NlaIV GGNNCC 2 cut(s) 277, 558
NmeAIII GCCGAG 2 cut(s) 129, 789
NmuCI GTSAC 1 cut(s) 344
NspI RCATGY 3 cut(s) 451, 851, 982
PagI TCATGA 1 cut(s) 1149
PciI ACATGT 2 cut(s) 447, 978
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 652
PdiI GCCGGC 1 cut(s) 650
PdmI GAANNNNTTC 1 cut(s) 1031
PfeI GAWTC 2 cut(s) 676, 1089
Pfl23II CGTACG 1 cut(s) 492
PflFI GACNNNGTC 1 cut(s) 34
PflMI CCANNNNNTGG 1 cut(s) 589
PkrI GCNGC 4 cut(s) 264, 372, 381, 1180
PscI ACATGT 2 cut(s) 447, 978
PshBI ATTAAT 1 cut(s) 17
Psp6I CCWGG 3 cut(s) 233, 278, 1067
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 3 cut(s) 233, 278, 1067
PspLI CGTACG 1 cut(s) 492
PspN4I GGNNCC 2 cut(s) 277, 558
PspPI GGNCC 1 cut(s) 282
PstI CTGCAG 2 cut(s) 381, 740
PstNI CAGNNNCTG 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 34
RsaI GTAC 8 cut(s) 5, 27, 88, 494, 617, 734, 760, 983
RsaNI GTAC 8 cut(s) 4, 26, 87, 493, 616, 733, 759, 982
RseI CAYNNNNRTG 1 cut(s) 223
SacII CCGCGG 1 cut(s) 266
SaqAI TTAA 2 cut(s) 17, 938
SatI GCNGC 4 cut(s) 263, 371, 380, 1179
Sau3AI GATC 3 cut(s) 270, 792, 1017
Sau96I GGNCC 1 cut(s) 282
ScrFI CCNGG 3 cut(s) 235, 280, 1069
SfaNI GCATC 2 cut(s) 555, 639
SfcI CTRYAG 2 cut(s) 377, 736
Sfr303I CCGCGG 1 cut(s) 266
SgrAI CRCCGGYG 1 cut(s) 648
SgrBI CCGCGG 1 cut(s) 266
SmiMI CAYNNNNRTG 1 cut(s) 223
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 6 cut(s) 18, 453, 468, 763, 816, 1145
SsiI CCGC 4 cut(s) 248, 263, 265, 1179
SspI AATATT 2 cut(s) 82, 685
SspMI CTAG 4 cut(s) 342, 756, 1194, 1216
StyD4I CCNGG 3 cut(s) 233, 278, 1067
StyI CCWWGG 1 cut(s) 1036
TaaI ACNGT 3 cut(s) 443, 620, 667
TaiI ACGT 2 cut(s) 591, 783
TaqI TCGA 1 cut(s) 396
TasI AATT 6 cut(s) 18, 453, 468, 763, 816, 1145
TatI WGTACW 4 cut(s) 3, 86, 615, 758
TauI GCSGC 2 cut(s) 265, 1181
TfiI GAWTC 2 cut(s) 676, 1089
Tru1I TTAA 2 cut(s) 17, 938
Tru9I TTAA 2 cut(s) 17, 938
TscAI CASTG 2 cut(s) 67, 1104
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 2 cut(s) 370, 379
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 6 cut(s) 119, 549, 886, 1138, 1166, 1178
TspRI CASTG 2 cut(s) 67, 1104
Tth111I GACNNNGTC 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 589
VspI ATTAAT 1 cut(s) 17
XapI RAATTY 1 cut(s) 1145
XceI RCATGY 3 cut(s) 451, 851, 982
XcmI CCANNNNNNNNNTGG 1 cut(s) 1065
XmnI GAANNNNTTC 1 cut(s) 1031
XspI CTAG 4 cut(s) 342, 756, 1194, 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.