Rw5G025730

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015575
Physical Location & Seq
Forward (+)
36822659 .. 36824508
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025730.1

Sequence Viewer

Length: 1230 bp
ATGTGCTGGTGTGCATTAATTCTCGTACTGACCTTGTCCTCCTCCTCTCTCGTCACCACTGCTTATAAGAGAGCCAATATTTGTACTCCCATCAACCCCATGAACATCACCAACTGCAAGAAACTACTCACTTTGGGAGCTGAACTCGGCTGGAACATCCACAACGAAACCCACATTGACATCTTGTTCACCATTCCAATATCTATGGACGACACACCAAAATGGGTCGCCTGGGGTGTGAACCCGAGTCGGAAAAGGCCGCGGATGATCGGAACCAGGGCCATTATCGCCGTTTCACAGGCCAACGGGACTTTGAAGGTCAGCAGATACAACATCACCAGTGACACCAAGCTGGGATGTCGTTTGCAGCCTCTGGAGCCTTCCAAAGAGTTCGAGGATGTCGTCGTCCAAAACATGACAGGTGATGTGAAACCTAACAGATACATGTCAATTTTTGCGACGCTAATTTTGCGTCCAGATTTAGCTCATGCGTACGACATTGCAAGGTTGAACCATGTGTGGCAAGTTGGGTATGAAGCTGATGAGGTTGCTCTGGAGCCTAAGATGCACCCGACGGCTCTCCAGAACGTGGATAGCACAGAGACTACAAACTTGCACAGTTGGAAAGGTATCAGCATTGGACATCGCCGGCATCATCTTAGAACGGTGCATGGGATTCTGAATATTGTGGGATGGGGAACATTGTTGCCCTTTGGAGTGATTATAGCGAGGTACTGCAGAAGATATCCCTTTCAATGTACACAATGGTTTTTTTCGCACGTCTCTTGTCAGATCGTTGGTTACATTCTCGGCACGATTGGTTGGGCAATCGGAATATGGCTCGGACATGCTTCCAAAGAATATCACTTCCACACTCACAATATTCTCGCCATGTTCATATTTGCATTTACAACATTACAAATGATTGCTCTGCGTTTAAGGCCAACGCCAACAGATGATTACCGGAAATACTGGGACATGTACCATCATTTTCTGGGCTACGCACTTTTGGCTGTGATCATATTGAATATATTCCATGGCATTGCCATTTTGAAGCCAGACAAAACCTGGCTATGGGCTTATATTGGAATCCTTGCAGTGCTTGCTTCCATCACATTGGGTTTGGAGATTTATACTTGGTGTAAATTCATGAAATCCAAAATGAAGAAAGCAAACACTTCCGGAGACCAAGTTCAACCTGTTAGTAGTAGTCAGTCTCATACCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

409

Amino Acids

46.46

Weight (kDa)

9.31

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 89 - 205 6.2e-18 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015575)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23200
malus_domestica MD03G1196700.v1.1 MD11G1212800.v1.1
prunus_persica Prupe.4G218700_v2.0.a1
pyrus_communis pycom11g18780
rosa_laevigata RLG00000034009
rosa_multiflora Rmu_sc0004953.1_g000005 Rmu_sc0004953.1_g000008
rosa_roxburghii Rroxscaffold_1G00039840
rosa_rugosa Rorug05G0188200 Rorug05G0220100
rosa_samantha Rh5AG273700 Rh5BG278800 Rh5CG310800 Rh5DG287000
rosa_wichuraiana Rw5G025730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
AccB7I CCANNNNNTGG 1 cut(s) 589
AccII CGCG 1 cut(s) 262
AccIII TCCGGA 1 cut(s) 1181
AciI CCGC 2 cut(s) 260, 262
AcsI RAATTY 1 cut(s) 1145
AfaI GTAC 6 cut(s) 27, 85, 494, 734, 760, 983
AfiI CCNNNNNNNGG 2 cut(s) 589, 1074
AflIII ACRYGT 2 cut(s) 444, 978
AgsI TTSAA 6 cut(s) 316, 511, 755, 1027, 1054, 1196
AjiI CACGTC 1 cut(s) 781
AjnI CCWGG 3 cut(s) 230, 275, 1067
AloI GAACNNNNNNTCC 2 cut(s) 1176, 1208
AluBI AGCT 4 cut(s) 140, 352, 485, 539
AluI AGCT 4 cut(s) 140, 352, 485, 539
Alw26I GTCTC 4 cut(s) 596, 787, 1179, 1221
AlwNI CAGNNNCTG 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 244
Aor13HI TCCGGA 1 cut(s) 1181
AoxI GGCC 4 cut(s) 257, 279, 300, 941
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 1 cut(s) 1145
ArsI GACNNNNNNTTYG 2 cut(s) 451, 483
AseI ATTAAT 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 1031
AspS9I GGNCC 1 cut(s) 279
AsuHPI GGTGA 5 cut(s) 46, 100, 181, 328, 434
AvaI CYCGRG 1 cut(s) 244
BbvI GCAGC 1 cut(s) 379
BccI CCATC 4 cut(s) 98, 687, 993, 1118
BceAI ACGGC 2 cut(s) 275, 591
BciT130I CCWGG 3 cut(s) 232, 277, 1069
BclI TGATCA 1 cut(s) 1017
BcoDI GTCTC 4 cut(s) 596, 787, 1179, 1221
BfmI CTRYAG 1 cut(s) 736
BisI GCNGC 2 cut(s) 260, 368
BlsI GCNGC 2 cut(s) 261, 369
Bme1390I CCNGG 3 cut(s) 232, 277, 1069
BmeT110I CYCGRG 1 cut(s) 244
BmgBI CACGTC 1 cut(s) 781
BmgT120I GGNCC 1 cut(s) 279
BmiI GGNNCC 3 cut(s) 274, 378, 558
BmrFI CCNGG 3 cut(s) 232, 277, 1069
BmrI ACTGGG 1 cut(s) 982
BmsI GCATC 2 cut(s) 555, 661
BmuI ACTGGG 1 cut(s) 982
BplI GAGNNNNNCTC 2 cut(s) 129, 161
BpmI CTGGAG 3 cut(s) 395, 566, 575
BsaI GGTCTC 1 cut(s) 1179
BsaJI CCNNGG 4 cut(s) 231, 260, 276, 1036
BsaWI WCCGGW 2 cut(s) 963, 1181
BsaXI ACNNNNNCTCC 2 cut(s) 1176, 1206
Bsc4I CCNNNNNNNGG 2 cut(s) 589, 1074
Bse118I RCCGGY 1 cut(s) 648
Bse1I ACTGG 2 cut(s) 339, 977
Bse3DI GCAATG 2 cut(s) 498, 1041
BseAI TCCGGA 1 cut(s) 1181
BseBI CCWGG 3 cut(s) 232, 277, 1069
BseDI CCNNGG 4 cut(s) 231, 260, 276, 1036
BseGI GGATG 5 cut(s) 156, 270, 362, 403, 698
BseLI CCNNNNNNNGG 2 cut(s) 589, 1074
BseMI GCAATG 2 cut(s) 498, 1041
BseNI ACTGG 2 cut(s) 339, 977
BseRI GAGGAG 2 cut(s) 31, 34
BseXI GCAGC 1 cut(s) 379
BseYI CCCAGC 1 cut(s) 352
Bsh1236I CGCG 1 cut(s) 262
BshFI GGCC 4 cut(s) 259, 281, 302, 943
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 3 cut(s) 649, 964, 1182
BsiWI CGTACG 1 cut(s) 492
BslFI GGGAC 2 cut(s) 322, 989
BslI CCNNNNNNNGG 2 cut(s) 589, 1074
BsmAI GTCTC 4 cut(s) 596, 787, 1179, 1221
BsmBI CGTCTC 1 cut(s) 787
BsmFI GGGAC 2 cut(s) 322, 989
BsnI GGCC 4 cut(s) 259, 281, 302, 943
Bso31I GGTCTC 1 cut(s) 1179
BsoBI CYCGRG 1 cut(s) 244
Bsp13I TCCGGA 1 cut(s) 1181
Bsp1407I TGTACA 1 cut(s) 758
Bsp143I GATC 3 cut(s) 267, 792, 1017
Bsp19I CCATGG 1 cut(s) 1036
BspACI CCGC 2 cut(s) 260, 262
BspANI GGCC 4 cut(s) 259, 281, 302, 943
BspEI TCCGGA 1 cut(s) 1181
BspFNI CGCG 1 cut(s) 262
BspHI TCATGA 1 cut(s) 1149
BspLI GGNNCC 3 cut(s) 274, 378, 558
BspMAI CTGCAG 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 1179
BsrDI GCAATG 2 cut(s) 498, 1041
BsrFI RCCGGY 1 cut(s) 648
BsrGI TGTACA 1 cut(s) 758
BsrI ACTGG 2 cut(s) 339, 977
BssAI RCCGGY 1 cut(s) 648
BssECI CCNNGG 4 cut(s) 231, 260, 276, 1036
BssMI GATC 3 cut(s) 267, 792, 1017
BssT1I CCWWGG 1 cut(s) 1036
Bst2UI CCWGG 3 cut(s) 232, 277, 1069
Bst4CI ACNGT 2 cut(s) 620, 667
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstAUI TGTACA 1 cut(s) 758
BstC8I GCNNGC 2 cut(s) 650, 1104
BstDEI CTNAG 3 cut(s) 561, 659, 1227
BstDSI CCRYGG 2 cut(s) 260, 1036
BstF5I GGATG 5 cut(s) 156, 270, 362, 403, 698
BstFNI CGCG 1 cut(s) 262
BstKTI GATC 3 cut(s) 270, 795, 1020
BstMAI GTCTC 4 cut(s) 596, 787, 1179, 1221
BstMBI GATC 3 cut(s) 267, 792, 1017
BstMWI GCNNNNNNNGC 7 cut(s) 287, 376, 469, 565, 940, 1010, 1103
BstNI CCWGG 3 cut(s) 232, 277, 1069
BstNSI RCATGY 3 cut(s) 448, 851, 982
BstSCI CCNGG 3 cut(s) 230, 275, 1067
BstSFI CTRYAG 1 cut(s) 736
BstUI CGCG 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 379
BstXI CCANNNNNNTGG 1 cut(s) 1117
BsuRI GGCC 4 cut(s) 259, 281, 302, 943
BtgI CCRYGG 2 cut(s) 260, 1036
BtgZI GCGATG 1 cut(s) 629
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 5 cut(s) 156, 270, 362, 403, 698
BtsI GCAGTG 2 cut(s) 57, 1104
BtsIMutI CAGTG 3 cut(s) 57, 346, 1104
Cac8I GCNNGC 2 cut(s) 650, 1104
CaiI CAGNNNCTG 1 cut(s) 373
CciI TCATGA 1 cut(s) 1149
Cfr10I RCCGGY 1 cut(s) 648
Cfr13I GGNCC 1 cut(s) 279
Cfr42I CCGCGG 1 cut(s) 263
CseI GACGC 2 cut(s) 461, 469
Csp6I GTAC 6 cut(s) 26, 84, 493, 733, 759, 982
CviQI GTAC 6 cut(s) 26, 84, 493, 733, 759, 982
DdeI CTNAG 3 cut(s) 561, 659, 1227
DpnI GATC 3 cut(s) 269, 794, 1019
DpnII GATC 3 cut(s) 267, 792, 1017
Eco130I CCWWGG 1 cut(s) 1036
Eco31I GGTCTC 1 cut(s) 1179
Eco32I GATATC 1 cut(s) 746
Eco88I CYCGRG 1 cut(s) 244
EcoRII CCWGG 3 cut(s) 230, 275, 1067
EcoRV GATATC 1 cut(s) 746
EcoT14I CCWWGG 1 cut(s) 1036
ErhI CCWWGG 1 cut(s) 1036
Esp3I CGTCTC 1 cut(s) 787
FaqI GGGAC 2 cut(s) 322, 989
FbaI TGATCA 1 cut(s) 1017
Fnu4HI GCNGC 2 cut(s) 260, 368
FokI GGATG 5 cut(s) 143, 277, 369, 410, 705
Fsp4HI GCNGC 2 cut(s) 260, 368
GluI GCNGC 2 cut(s) 260, 368
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 3 cut(s) 395, 566, 575
HaeIII GGCC 4 cut(s) 259, 281, 302, 943
HapII CCGG 3 cut(s) 649, 964, 1182
HgaI GACGC 2 cut(s) 461, 469
HinfI GANTC 3 cut(s) 247, 676, 1089
HpaII CCGG 3 cut(s) 649, 964, 1182
HphI GGTGA 5 cut(s) 46, 100, 181, 328, 434
Hpy166II GTNNAC 3 cut(s) 189, 241, 761
Hpy188I TCNGA 6 cut(s) 252, 272, 681, 792, 833, 845
Hpy188III TCNNGA 6 cut(s) 374, 476, 554, 583, 1150, 1182
Hpy8I GTNNAC 3 cut(s) 189, 241, 761
Hpy99I CGWCG 3 cut(s) 407, 463, 577
HpyAV CCTTC 2 cut(s) 310, 390
HpyCH4III ACNGT 2 cut(s) 620, 667
HpyCH4IV ACGT 2 cut(s) 588, 780
HpyF10VI GCNNNNNNNGC 7 cut(s) 287, 376, 469, 565, 940, 1010, 1103
HpyF3I CTNAG 3 cut(s) 561, 659, 1227
HpySE526I ACGT 2 cut(s) 588, 780
Kpn2I TCCGGA 1 cut(s) 1181
KroI GCCGGC 1 cut(s) 648
KroNI GCCGGC 1 cut(s) 650
Ksp22I TGATCA 1 cut(s) 1017
KspI CCGCGG 1 cut(s) 263
Kzo9I GATC 3 cut(s) 267, 792, 1017
LmnI GCTCC 3 cut(s) 137, 376, 556
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 2 cut(s) 555, 661
MaeII ACGT 2 cut(s) 588, 780
MaeIII GTNAC 3 cut(s) 52, 341, 800
MalI GATC 3 cut(s) 269, 794, 1019
MboI GATC 3 cut(s) 267, 792, 1017
MboII GAAGA 2 cut(s) 753, 1177
MluCI AATT 4 cut(s) 18, 450, 465, 1145
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 2 cut(s) 230, 602
MnlI CCTC 7 cut(s) 49, 52, 55, 381, 388, 538, 723
MroI TCCGGA 1 cut(s) 1181
MroNI GCCGGC 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 1031
MseI TTAA 2 cut(s) 17, 938
MslI CAYNNNNRTG 1 cut(s) 220
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 3 cut(s) 649, 964, 1182
MspR9I CCNGG 3 cut(s) 232, 277, 1069
MvaI CCWGG 3 cut(s) 232, 277, 1069
MvnI CGCG 1 cut(s) 262
MwoI GCNNNNNNNGC 7 cut(s) 287, 376, 469, 565, 940, 1010, 1103
NaeI GCCGGC 1 cut(s) 650
NcoI CCATGG 1 cut(s) 1036
NdeII GATC 3 cut(s) 267, 792, 1017
NgoMIV GCCGGC 1 cut(s) 648
NlaIV GGNNCC 3 cut(s) 274, 378, 558
NmeAIII GCCGAG 2 cut(s) 126, 789
NmuCI GTSAC 2 cut(s) 52, 341
NspI RCATGY 3 cut(s) 448, 851, 982
PagI TCATGA 1 cut(s) 1149
PciI ACATGT 2 cut(s) 444, 978
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PdiI GCCGGC 1 cut(s) 650
PdmI GAANNNNTTC 1 cut(s) 1031
PfeI GAWTC 2 cut(s) 676, 1089
Pfl23II CGTACG 1 cut(s) 492
PflFI GACNNNGTC 1 cut(s) 34
PflMI CCANNNNNTGG 1 cut(s) 589
PkrI GCNGC 2 cut(s) 261, 369
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PscI ACATGT 2 cut(s) 444, 978
PshBI ATTAAT 1 cut(s) 17
PsiI TTATAA 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 230, 275, 1067
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 3 cut(s) 230, 275, 1067
PspLI CGTACG 1 cut(s) 492
PspN4I GGNNCC 3 cut(s) 274, 378, 558
PspPI GGNCC 1 cut(s) 279
PstI CTGCAG 1 cut(s) 740
PstNI CAGNNNCTG 1 cut(s) 373
PsyI GACNNNGTC 1 cut(s) 34
RsaI GTAC 6 cut(s) 27, 85, 494, 734, 760, 983
RsaNI GTAC 6 cut(s) 26, 84, 493, 733, 759, 982
RseI CAYNNNNRTG 1 cut(s) 220
SacII CCGCGG 1 cut(s) 263
SaqAI TTAA 2 cut(s) 17, 938
SatI GCNGC 2 cut(s) 260, 368
Sau3AI GATC 3 cut(s) 267, 792, 1017
Sau96I GGNCC 1 cut(s) 279
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 3 cut(s) 232, 277, 1069
SfaNI GCATC 2 cut(s) 555, 661
SfcI CTRYAG 1 cut(s) 736
Sfr303I CCGCGG 1 cut(s) 263
SgrBI CCGCGG 1 cut(s) 263
SmiMI CAYNNNNRTG 1 cut(s) 220
Sse9I AATT 4 cut(s) 18, 450, 465, 1145
SsiI CCGC 2 cut(s) 260, 262
SspI AATATT 3 cut(s) 79, 685, 883
StyD4I CCNGG 3 cut(s) 230, 275, 1067
StyI CCWWGG 1 cut(s) 1036
TaaI ACNGT 2 cut(s) 620, 667
TaiI ACGT 2 cut(s) 591, 783
TaqI TCGA 1 cut(s) 393
TasI AATT 4 cut(s) 18, 450, 465, 1145
TatI WGTACW 2 cut(s) 83, 758
TauI GCSGC 1 cut(s) 262
TfiI GAWTC 2 cut(s) 676, 1089
Tru1I TTAA 2 cut(s) 17, 938
Tru9I TTAA 2 cut(s) 17, 938
TscAI CASTG 3 cut(s) 64, 346, 1104
TseFI GTSAC 2 cut(s) 52, 341
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 2 cut(s) 52, 341
TspDTI ATGAA 6 cut(s) 116, 549, 886, 1138, 1166, 1178
TspRI CASTG 3 cut(s) 64, 346, 1104
Tth111I GACNNNGTC 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 589
VspI ATTAAT 1 cut(s) 17
XapI RAATTY 1 cut(s) 1145
XceI RCATGY 3 cut(s) 448, 851, 982
XcmI CCANNNNNNNNNTGG 1 cut(s) 1065
XmnI GAANNNNTTC 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.