Rh5DG287000

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015575
Physical Location & Seq
Forward (+)
35655476 .. 35657224
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG287000.1

Sequence Viewer

Length: 1128 bp
ATGAACATCACCAACTGCAAGAAACTACTCACTTTGGGAGCTGAACTCGGCTGGAACATCCACAACGAAACCCACATTGACATCTTGTTCACCATTCCAATATCTATGGACGACACACCAAAATGGGTCGCCTGGGGTGTGAACCCGCGTCGGAAAAGGCCGCGGATGATCGGAACCAGGGCCATTATCGCCGTTTCACAGGCCAACGGGACTTTGAAGGTCAGCAGATACAACATCACCAGTGACACCAAGCTGGGATGTCGTTTGCAGCCTCTGGAGCCTTCCAAAGAGTTCGAGGATGTCGTCGTGCAAAACATGACAGGTGATGTGAAACCTAACAGATACATGTCAATTTTTGCGACGCTAATTTTGCGTCCAGATTTAGCTCATGCGTACGACATTGCAAGGTTGAACCATGTGTGGCAAGTTGGGTATGAAGCTGATGAGGTTGCTCTGGAGCCTAAGATGCACCCGACGGCTCTCCAGAACGTGGATAGCACAGAGACTACAAACTTGCACAGTCGGAAAGGTATCAGCATTGGACATCGCCGGCGTCATCTTAGAACTGTGCATGGGATTCTGAATATTGTGGGATGGGGAACATTGTTGCCCTTTGGAGTGATTATAGCGAGGTACTGCAGAAGATATCCCTTTCAATGTACACATTGGTTTTTATCGCACGTCTCTTGTCAGATCGTTGGTTACATTCTCGGCACGATTGGTTGGGCAATCGGAATATGGCTCGGACATGCTTCCAAAGAATATCACTTCCACACTCACAATATTCTCGCCATGTTCATATTTGCATTTACAACATTACAAATGATTGCTCTGCGTTTAAGGCCAACGCCAACAGATGATTACCGGAAATACTGGGACATGTACCATCATTTTCTGGGCTACGCACTTTTGGCTGTGATCATATTGAATATATTCCATGGCATTGCCATTTTGAAGCCAGACAAAACCTGGCTATGGGCTTATATTGGAATCCTTGCAGTGCTTGCTTCCATCACATTGGGTTTGGAGATTTATACTTGGTGTAAATTCATGAAATCCAAAATGAAGAAAGCAAACAATTCCGGAGAAGTTCAACCTGCAGTTAGTAGTCAGTCTCATACCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

375

Amino Acids

42.81

Weight (kDa)

9.53

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 56 - 172 5.3e-18 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015575)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23200
malus_domestica MD03G1196700.v1.1 MD11G1212800.v1.1
prunus_persica Prupe.4G218700_v2.0.a1
pyrus_communis pycom11g18780
rosa_laevigata RLG00000034009
rosa_multiflora Rmu_sc0004953.1_g000005 Rmu_sc0004953.1_g000008
rosa_roxburghii Rroxscaffold_1G00039840
rosa_rugosa Rorug05G0188200 Rorug05G0220100
rosa_samantha Rh5AG273700 Rh5BG278800 Rh5CG310800 Rh5DG287000
rosa_wichuraiana Rw5G025730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1105
AccB7I CCANNNNNTGG 1 cut(s) 490
AccII CGCG 2 cut(s) 148, 163
AccIII TCCGGA 1 cut(s) 1082
AciI CCGC 3 cut(s) 146, 161, 163
AcsI RAATTY 1 cut(s) 1046
AcyI GRCGYC 1 cut(s) 553
AfaI GTAC 4 cut(s) 395, 635, 661, 884
AfiI CCNNNNNNNGG 2 cut(s) 490, 975
AflIII ACRYGT 2 cut(s) 345, 879
AgsI TTSAA 6 cut(s) 217, 412, 656, 928, 955, 1094
AjiI CACGTC 1 cut(s) 682
AjnI CCWGG 3 cut(s) 131, 176, 968
AluBI AGCT 4 cut(s) 41, 253, 386, 440
AluI AGCT 4 cut(s) 41, 253, 386, 440
Alw26I GTCTC 3 cut(s) 497, 688, 1119
AlwNI CAGNNNCTG 1 cut(s) 274
Aor13HI TCCGGA 1 cut(s) 1082
AoxI GGCC 4 cut(s) 158, 180, 201, 842
ApeKI GCWGC 1 cut(s) 268
ApoI RAATTY 1 cut(s) 1046
ArsI GACNNNNNNTTYG 2 cut(s) 352, 384
Asp700I GAANNNNTTC 1 cut(s) 932
AspS9I GGNCC 1 cut(s) 180
AsuHPI GGTGA 3 cut(s) 82, 229, 335
BbvI GCAGC 1 cut(s) 280
BccI CCATC 3 cut(s) 588, 894, 1019
BceAI ACGGC 2 cut(s) 176, 492
BciT130I CCWGG 3 cut(s) 133, 178, 970
BclI TGATCA 1 cut(s) 918
BcoDI GTCTC 3 cut(s) 497, 688, 1119
BfmI CTRYAG 2 cut(s) 637, 1098
BfuAI ACCTGC 1 cut(s) 1105
BisI GCNGC 2 cut(s) 161, 269
BlsI GCNGC 2 cut(s) 162, 270
Bme1390I CCNGG 3 cut(s) 133, 178, 970
BmgBI CACGTC 1 cut(s) 682
BmgT120I GGNCC 1 cut(s) 180
BmiI GGNNCC 3 cut(s) 175, 279, 459
BmrFI CCNGG 3 cut(s) 133, 178, 970
BmrI ACTGGG 1 cut(s) 883
BmsI GCATC 1 cut(s) 456
BmuI ACTGGG 1 cut(s) 883
BplI GAGNNNNNCTC 2 cut(s) 30, 62
BpmI CTGGAG 3 cut(s) 296, 467, 476
BsaHI GRCGYC 1 cut(s) 553
BsaJI CCNNGG 4 cut(s) 132, 161, 177, 937
BsaWI WCCGGW 2 cut(s) 864, 1082
Bsc4I CCNNNNNNNGG 2 cut(s) 490, 975
Bse118I RCCGGY 1 cut(s) 549
Bse1I ACTGG 2 cut(s) 240, 878
Bse3DI GCAATG 2 cut(s) 399, 942
BseAI TCCGGA 1 cut(s) 1082
BseBI CCWGG 3 cut(s) 133, 178, 970
BseDI CCNNGG 4 cut(s) 132, 161, 177, 937
BseGI GGATG 5 cut(s) 57, 171, 263, 304, 599
BseLI CCNNNNNNNGG 2 cut(s) 490, 975
BseMI GCAATG 2 cut(s) 399, 942
BseNI ACTGG 2 cut(s) 240, 878
BseXI GCAGC 1 cut(s) 280
BseYI CCCAGC 1 cut(s) 253
Bsh1236I CGCG 2 cut(s) 148, 163
BshFI GGCC 4 cut(s) 160, 182, 203, 844
BsiSI CCGG 3 cut(s) 550, 865, 1083
BsiWI CGTACG 1 cut(s) 393
BslFI GGGAC 2 cut(s) 223, 890
BslI CCNNNNNNNGG 2 cut(s) 490, 975
BsmAI GTCTC 3 cut(s) 497, 688, 1119
BsmBI CGTCTC 1 cut(s) 688
BsmFI GGGAC 2 cut(s) 223, 890
BsnI GGCC 4 cut(s) 160, 182, 203, 844
Bsp13I TCCGGA 1 cut(s) 1082
Bsp1407I TGTACA 1 cut(s) 659
Bsp143I GATC 3 cut(s) 168, 693, 918
Bsp19I CCATGG 1 cut(s) 937
BspACI CCGC 3 cut(s) 146, 161, 163
BspANI GGCC 4 cut(s) 160, 182, 203, 844
BspEI TCCGGA 1 cut(s) 1082
BspFNI CGCG 2 cut(s) 148, 163
BspHI TCATGA 1 cut(s) 1050
BspLI GGNNCC 3 cut(s) 175, 279, 459
BspMAI CTGCAG 2 cut(s) 641, 1102
BspMI ACCTGC 1 cut(s) 1105
BsrDI GCAATG 2 cut(s) 399, 942
BsrFI RCCGGY 1 cut(s) 549
BsrGI TGTACA 1 cut(s) 659
BsrI ACTGG 2 cut(s) 240, 878
BssAI RCCGGY 1 cut(s) 549
BssECI CCNNGG 4 cut(s) 132, 161, 177, 937
BssMI GATC 3 cut(s) 168, 693, 918
BssNI GRCGYC 1 cut(s) 553
BssT1I CCWWGG 1 cut(s) 937
Bst2UI CCWGG 3 cut(s) 133, 178, 970
Bst4CI ACNGT 2 cut(s) 521, 568
BstACI GRCGYC 1 cut(s) 553
BstAPI GCANNNNNTGC 1 cut(s) 1004
BstAUI TGTACA 1 cut(s) 659
BstC8I GCNNGC 2 cut(s) 551, 1005
BstDEI CTNAG 3 cut(s) 462, 560, 1125
BstDSI CCRYGG 2 cut(s) 161, 937
BstF5I GGATG 5 cut(s) 57, 171, 263, 304, 599
BstFNI CGCG 2 cut(s) 148, 163
BstKTI GATC 3 cut(s) 171, 696, 921
BstMAI GTCTC 3 cut(s) 497, 688, 1119
BstMBI GATC 3 cut(s) 168, 693, 918
BstMWI GCNNNNNNNGC 7 cut(s) 188, 277, 370, 466, 841, 911, 1004
BstNI CCWGG 3 cut(s) 133, 178, 970
BstNSI RCATGY 3 cut(s) 349, 752, 883
BstSCI CCNGG 3 cut(s) 131, 176, 968
BstSFI CTRYAG 2 cut(s) 637, 1098
BstUI CGCG 2 cut(s) 148, 163
BstV1I GCAGC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 1018
BsuRI GGCC 4 cut(s) 160, 182, 203, 844
BtgI CCRYGG 2 cut(s) 161, 937
BtgZI GCGATG 1 cut(s) 530
BtrI CACGTC 1 cut(s) 682
BtsCI GGATG 5 cut(s) 57, 171, 263, 304, 599
BtsI GCAGTG 1 cut(s) 1005
BtsIMutI CAGTG 2 cut(s) 247, 1005
BveI ACCTGC 1 cut(s) 1105
Cac8I GCNNGC 2 cut(s) 551, 1005
CaiI CAGNNNCTG 1 cut(s) 274
CciI TCATGA 1 cut(s) 1050
Cfr10I RCCGGY 1 cut(s) 549
Cfr13I GGNCC 1 cut(s) 180
Cfr42I CCGCGG 1 cut(s) 164
CseI GACGC 4 cut(s) 137, 362, 370, 542
Csp6I GTAC 4 cut(s) 394, 634, 660, 883
CviQI GTAC 4 cut(s) 394, 634, 660, 883
DdeI CTNAG 3 cut(s) 462, 560, 1125
DpnI GATC 3 cut(s) 170, 695, 920
DpnII GATC 3 cut(s) 168, 693, 918
Eco130I CCWWGG 1 cut(s) 937
Eco32I GATATC 1 cut(s) 647
EcoRII CCWGG 3 cut(s) 131, 176, 968
EcoRV GATATC 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 937
ErhI CCWWGG 1 cut(s) 937
Esp3I CGTCTC 1 cut(s) 688
FaqI GGGAC 2 cut(s) 223, 890
FauI CCCGC 1 cut(s) 153
FbaI TGATCA 1 cut(s) 918
Fnu4HI GCNGC 2 cut(s) 161, 269
FokI GGATG 5 cut(s) 44, 178, 270, 311, 606
Fsp4HI GCNGC 2 cut(s) 161, 269
GluI GCNGC 2 cut(s) 161, 269
GsaI CCCAGC 1 cut(s) 257
GsuI CTGGAG 3 cut(s) 296, 467, 476
HaeIII GGCC 4 cut(s) 160, 182, 203, 844
HapII CCGG 3 cut(s) 550, 865, 1083
HgaI GACGC 4 cut(s) 137, 362, 370, 542
Hin1I GRCGYC 1 cut(s) 553
HinfI GANTC 2 cut(s) 577, 990
HpaII CCGG 3 cut(s) 550, 865, 1083
HphI GGTGA 3 cut(s) 82, 229, 335
Hpy166II GTNNAC 3 cut(s) 90, 142, 662
Hpy188I TCNGA 7 cut(s) 153, 173, 525, 582, 693, 734, 746
Hpy188III TCNNGA 6 cut(s) 275, 377, 455, 484, 1051, 1083
Hpy8I GTNNAC 3 cut(s) 90, 142, 662
Hpy99I CGWCG 4 cut(s) 153, 308, 364, 478
HpyAV CCTTC 2 cut(s) 211, 291
HpyCH4III ACNGT 2 cut(s) 521, 568
HpyCH4IV ACGT 2 cut(s) 489, 681
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 277, 370, 466, 841, 911, 1004
HpyF3I CTNAG 3 cut(s) 462, 560, 1125
HpySE526I ACGT 2 cut(s) 489, 681
Hsp92I GRCGYC 1 cut(s) 553
Kpn2I TCCGGA 1 cut(s) 1082
KroI GCCGGC 1 cut(s) 549
KroNI GCCGGC 1 cut(s) 551
Ksp22I TGATCA 1 cut(s) 918
KspI CCGCGG 1 cut(s) 164
Kzo9I GATC 3 cut(s) 168, 693, 918
LmnI GCTCC 3 cut(s) 38, 277, 457
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 1 cut(s) 456
MaeII ACGT 2 cut(s) 489, 681
MaeIII GTNAC 2 cut(s) 242, 701
MalI GATC 3 cut(s) 170, 695, 920
MboI GATC 3 cut(s) 168, 693, 918
MboII GAAGA 2 cut(s) 654, 1078
MluCI AATT 4 cut(s) 351, 366, 1046, 1078
MmeI TCCRAC 2 cut(s) 131, 503
MnlI CCTC 4 cut(s) 282, 289, 439, 624
MreI CGCCGGCG 1 cut(s) 549
MroI TCCGGA 1 cut(s) 1082
MroNI GCCGGC 1 cut(s) 549
MroXI GAANNNNTTC 1 cut(s) 932
MseI TTAA 1 cut(s) 839
MslI CAYNNNNRTG 1 cut(s) 121
MspA1I CMGCKG 1 cut(s) 163
MspI CCGG 3 cut(s) 550, 865, 1083
MspR9I CCNGG 3 cut(s) 133, 178, 970
MvaI CCWGG 3 cut(s) 133, 178, 970
MvnI CGCG 2 cut(s) 148, 163
MwoI GCNNNNNNNGC 7 cut(s) 188, 277, 370, 466, 841, 911, 1004
NaeI GCCGGC 1 cut(s) 551
NcoI CCATGG 1 cut(s) 937
NdeII GATC 3 cut(s) 168, 693, 918
NgoMIV GCCGGC 1 cut(s) 549
NlaIV GGNNCC 3 cut(s) 175, 279, 459
NmeAIII GCCGAG 2 cut(s) 27, 690
NmuCI GTSAC 1 cut(s) 242
NspI RCATGY 3 cut(s) 349, 752, 883
PagI TCATGA 1 cut(s) 1050
PciI ACATGT 2 cut(s) 345, 879
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PdiI GCCGGC 1 cut(s) 551
PdmI GAANNNNTTC 1 cut(s) 932
PfeI GAWTC 2 cut(s) 577, 990
Pfl23II CGTACG 1 cut(s) 393
PflMI CCANNNNNTGG 1 cut(s) 490
PkrI GCNGC 2 cut(s) 162, 270
PscI ACATGT 2 cut(s) 345, 879
Psp6I CCWGG 3 cut(s) 131, 176, 968
PspFI CCCAGC 1 cut(s) 253
PspGI CCWGG 3 cut(s) 131, 176, 968
PspLI CGTACG 1 cut(s) 393
PspN4I GGNNCC 3 cut(s) 175, 279, 459
PspPI GGNCC 1 cut(s) 180
PstI CTGCAG 2 cut(s) 641, 1102
PstNI CAGNNNCTG 1 cut(s) 274
RsaI GTAC 4 cut(s) 395, 635, 661, 884
RsaNI GTAC 4 cut(s) 394, 634, 660, 883
RseI CAYNNNNRTG 1 cut(s) 121
SacII CCGCGG 1 cut(s) 164
SaqAI TTAA 1 cut(s) 839
SatI GCNGC 2 cut(s) 161, 269
Sau3AI GATC 3 cut(s) 168, 693, 918
Sau96I GGNCC 1 cut(s) 180
ScrFI CCNGG 3 cut(s) 133, 178, 970
SfaNI GCATC 1 cut(s) 456
SfcI CTRYAG 2 cut(s) 637, 1098
Sfr303I CCGCGG 1 cut(s) 164
SgrAI CRCCGGYG 1 cut(s) 549
SgrBI CCGCGG 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 121
Sse9I AATT 4 cut(s) 351, 366, 1046, 1078
SsiI CCGC 3 cut(s) 146, 161, 163
SspI AATATT 2 cut(s) 586, 784
StyD4I CCNGG 3 cut(s) 131, 176, 968
StyI CCWWGG 1 cut(s) 937
TaaI ACNGT 2 cut(s) 521, 568
TaiI ACGT 2 cut(s) 492, 684
TaqI TCGA 1 cut(s) 294
TasI AATT 4 cut(s) 351, 366, 1046, 1078
TatI WGTACW 1 cut(s) 659
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 2 cut(s) 577, 990
Tru1I TTAA 1 cut(s) 839
Tru9I TTAA 1 cut(s) 839
TscAI CASTG 2 cut(s) 247, 1005
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 1 cut(s) 268
Tsp45I GTSAC 1 cut(s) 242
TspDTI ATGAA 6 cut(s) 17, 450, 787, 1039, 1067, 1079
TspRI CASTG 2 cut(s) 247, 1005
Van91I CCANNNNNTGG 1 cut(s) 490
XapI RAATTY 1 cut(s) 1046
XceI RCATGY 3 cut(s) 349, 752, 883
XcmI CCANNNNNNNNNTGG 1 cut(s) 966
XmnI GAANNNNTTC 1 cut(s) 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.