MD11G1212800.v1.1

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015575
Physical Location & Seq
Reverse (-)
31062733 .. 31065197
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1212800.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGCCGGCCACTACCAAATCTGTAATTCTCATCATATTAACAGTTTCCTCCTCACTTCTTTTCCCAATTTCTGAGCAATTTTCCAATTGTAGCCCCATCAATCCCATGAACATTACAAACTGCAAGAAACTAATCACTCTAGGAGCAGAATTCGGGTGGAACATTCACAGCAACACCGAAATCAACATCTTGTTCACCATTAGAATGCCTATTAAATGGATAGCCTGGGGTGTAAACCCAGGTTCCCAAAGACCTCAGATGGTAGGGACCAGGGCTATTATAGGTATTCAACAGTCCAATGGAACGTTGGCAGTTAGAAAATACAACATCACCAGTGACACCAAGCTGGGTTGTAGGCTTCAGCCTTCACAAGATTTTGATGATGTTATCGTCAAGAACATGAGAGGTGAAGTAAACCCTAGGTACATGTCGATATCCGCGACGTTAATTTTGCCCCGAGCTCCAGCTGTCTACGATATTTCGAAGCTGAATCATGTGTGGCAGGTAGGGTTTGAGGCTGATGATGTTCTTATGGAGCCTAAGATGCATTCCACTTCTCTCCAGAATGTGGATAGCACAGAGAGCATAAACATGATCAGCGGACGCGGTCTTAGCATAGGACATCGCCGGCATCACCTCAGGACGGTACATGGGATTCTTAATATTCTGGGGTGGGGAACAATGTTGCCATTGGGAGTGATCATAGCAAGGTACTTCAGAAAATACCCTGTTGACTGTGAAAAGTGGTATATAGTTCATGTCTCCTGCCAGATTGTTGGTTACACTCTTGGCACGGCTGGTTGGGCGATCGGACTCTGGCTCGGCCATGCTTCCAGACACTACAGCTTCTCCACTCATCGAATTCTCGCCATTTGCATATTTGCCTTCACCACATTACAAATGCTTGCCCTGCGTTTAAGGCCATCGGCAACTGATGACTACCGGAAATATTGGGACATGTACCATCATTTTCTAGGGTATGCACTGCTGGCTCTGATCTCAGTGAACATATTCCATGGCATTGCGATTTTGAAGCCGAATAAAACCTGGCTGTGGGTTTACATCGGAGTTCTTGGAATGTTTGCTTTGGTTGCGATTGGTTTGGAGATTTTTACTTGGCGTAAGTTCATGCATGCAAAGTCCAAAAGGAGGCAAACAGGTCAACCTGGTTCACAACCTCAAGGCTCCTCGGGCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

400

Amino Acids

44.93

Weight (kDa)

9.8

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 87 - 198 2.1e-18 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015575)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23200
malus_domestica MD03G1196700.v1.1 MD11G1212800.v1.1
prunus_persica Prupe.4G218700_v2.0.a1
pyrus_communis pycom11g18780
rosa_laevigata RLG00000034009
rosa_multiflora Rmu_sc0004953.1_g000005 Rmu_sc0004953.1_g000008
rosa_roxburghii Rroxscaffold_1G00039840
rosa_rugosa Rorug05G0188200 Rorug05G0220100
rosa_samantha Rh5AG273700 Rh5BG278800 Rh5CG310800 Rh5DG287000
rosa_wichuraiana Rw5G025730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 493
AccB7I CCANNNNNTGG 1 cut(s) 568
AccI GTMKAC 1 cut(s) 471
AccII CGCG 2 cut(s) 440, 606
AciI CCGC 3 cut(s) 438, 600, 606
AclI AACGTT 1 cut(s) 305
AcoI YGGCCR 2 cut(s) 6, 823
AcsI RAATTY 2 cut(s) 149, 861
AcuI CTGAAG 2 cut(s) 344, 700
AfaI GTAC 4 cut(s) 425, 648, 713, 962
AfiI CCNNNNNNNGG 4 cut(s) 568, 643, 1053, 1149
AflIII ACRYGT 2 cut(s) 426, 957
AgsI TTSAA 2 cut(s) 290, 1033
AjnI CCWGG 5 cut(s) 224, 238, 269, 1046, 1165
AluBI AGCT 5 cut(s) 346, 461, 467, 487, 846
AluI AGCT 5 cut(s) 346, 461, 467, 487, 846
Alw21I GWGCWC 1 cut(s) 463
Alw26I GTCTC 1 cut(s) 766
Ama87I CYCGRG 2 cut(s) 456, 1189
AoxI GGCC 3 cut(s) 6, 823, 920
ApoI RAATTY 2 cut(s) 149, 861
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
Asp700I GAANNNNTTC 1 cut(s) 1010
AspA2I CCTAGG 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 267
AsuHPI GGTGA 5 cut(s) 187, 322, 419, 626, 880
AsuII TTCGAA 1 cut(s) 482
AvaI CYCGRG 2 cut(s) 456, 1189
AvaII GGWCC 1 cut(s) 267
AvrII CCTAGG 1 cut(s) 419
AxyI CCTNAGG 1 cut(s) 638
BaeGI GKGCMC 1 cut(s) 1196
BanII GRGCYC 1 cut(s) 463
Bbv12I GWGCWC 1 cut(s) 463
BccI CCATC 4 cut(s) 104, 253, 931, 972
BceAI ACGGC 1 cut(s) 810
BciT130I CCWGG 5 cut(s) 226, 240, 271, 1048, 1167
BclI TGATCA 2 cut(s) 594, 699
BcoDI GTCTC 1 cut(s) 766
BfaI CTAG 3 cut(s) 140, 420, 974
BfmI CTRYAG 1 cut(s) 841
BfuAI ACCTGC 1 cut(s) 493
BlnI CCTAGG 1 cut(s) 419
Bme1390I CCNGG 5 cut(s) 226, 240, 271, 1048, 1167
Bme18I GGWCC 1 cut(s) 267
BmeT110I CYCGRG 2 cut(s) 456, 1189
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 4 cut(s) 244, 268, 537, 1186
BmrFI CCNGG 5 cut(s) 226, 240, 271, 1048, 1167
BmsI GCATC 2 cut(s) 534, 640
BpmI CTGGAG 2 cut(s) 447, 545
Bpu14I TTCGAA 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 1164
BsaJI CCNNGG 6 cut(s) 225, 238, 270, 419, 1015, 1188
BsaWI WCCGGW 1 cut(s) 942
BsaXI ACNNNNNCTCC 2 cut(s) 833, 863
Bsc4I CCNNNNNNNGG 4 cut(s) 568, 643, 1053, 1149
Bse118I RCCGGY 2 cut(s) 4, 627
Bse1I ACTGG 1 cut(s) 333
Bse21I CCTNAGG 1 cut(s) 638
Bse3DI GCAATG 1 cut(s) 1020
BseBI CCWGG 5 cut(s) 226, 240, 271, 1048, 1167
BseDI CCNNGG 6 cut(s) 225, 238, 270, 419, 1015, 1188
BseLI CCNNNNNNNGG 4 cut(s) 568, 643, 1053, 1149
BseMI GCAATG 1 cut(s) 1020
BseMII CTCAG 4 cut(s) 63, 269, 652, 1014
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 2 cut(s) 40, 1177
BseSI GKGCMC 1 cut(s) 1196
BseYI CCCAGC 1 cut(s) 346
Bsh1236I CGCG 2 cut(s) 440, 606
Bsh1285I CGRYCG 1 cut(s) 810
BshFI GGCC 3 cut(s) 8, 825, 922
BsiEI CGRYCG 1 cut(s) 810
BsiHKAI GWGCWC 1 cut(s) 463
BsiHKCI CYCGRG 2 cut(s) 456, 1189
BsiSI CCGG 3 cut(s) 5, 628, 943
BslFI GGGAC 2 cut(s) 280, 968
BslI CCNNNNNNNGG 4 cut(s) 568, 643, 1053, 1149
BsmAI GTCTC 1 cut(s) 766
BsmFI GGGAC 2 cut(s) 280, 968
BsmI GAATGC 2 cut(s) 210, 547
BsnI GGCC 3 cut(s) 8, 825, 922
BsoBI CYCGRG 2 cut(s) 456, 1189
Bsp119I TTCGAA 1 cut(s) 482
Bsp1286I GDGCHC 2 cut(s) 463, 1196
Bsp143I GATC 4 cut(s) 594, 699, 807, 996
Bsp19I CCATGG 1 cut(s) 1015
BspACI CCGC 3 cut(s) 438, 600, 606
BspANI GGCC 3 cut(s) 8, 825, 922
BspCNI CTCAG 4 cut(s) 64, 268, 651, 1013
BspFNI CGCG 2 cut(s) 440, 606
BspLI GGNNCC 4 cut(s) 244, 268, 537, 1186
BspMI ACCTGC 1 cut(s) 493
BspT104I TTCGAA 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 1020
BsrFI RCCGGY 2 cut(s) 4, 627
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 2 cut(s) 4, 627
BssECI CCNNGG 6 cut(s) 225, 238, 270, 419, 1015, 1188
BssMI GATC 4 cut(s) 594, 699, 807, 996
BssT1I CCWWGG 2 cut(s) 419, 1015
Bst2UI CCWGG 5 cut(s) 226, 240, 271, 1048, 1167
Bst4CI ACNGT 4 cut(s) 43, 294, 646, 737
BstBI TTCGAA 1 cut(s) 482
BstC8I GCNNGC 5 cut(s) 6, 629, 906, 990, 1134
BstDEI CTNAG 6 cut(s) 72, 255, 540, 611, 638, 1000
BstDSI CCRYGG 1 cut(s) 1015
BstFNI CGCG 2 cut(s) 440, 606
BstKTI GATC 4 cut(s) 597, 702, 810, 999
BstMAI GTCTC 1 cut(s) 766
BstMBI GATC 4 cut(s) 594, 699, 807, 996
BstMCI CGRYCG 1 cut(s) 810
BstMWI GCNNNNNNNGC 9 cut(s) 544, 582, 612, 803, 910, 919, 989, 1091, 1191
BstNI CCWGG 5 cut(s) 226, 240, 271, 1048, 1167
BstNSI RCATGY 3 cut(s) 430, 961, 1136
BstSCI CCNGG 5 cut(s) 224, 238, 269, 1046, 1165
BstSFI CTRYAG 1 cut(s) 841
BstSLI GKGCMC 1 cut(s) 1196
BstUI CGCG 2 cut(s) 440, 606
BstXI CCANNNNNNTGG 1 cut(s) 776
Bsu36I CCTNAGG 1 cut(s) 638
BsuRI GGCC 3 cut(s) 8, 825, 922
BtgI CCRYGG 1 cut(s) 1015
BtgZI GCGATG 1 cut(s) 608
BtsI GCAGTG 1 cut(s) 983
BtsIMutI CAGTG 3 cut(s) 340, 983, 1008
BveI ACCTGC 1 cut(s) 493
Cac8I GCNNGC 5 cut(s) 6, 629, 906, 990, 1134
Cfr10I RCCGGY 2 cut(s) 4, 627
Cfr13I GGNCC 1 cut(s) 267
CseI GACGC 1 cut(s) 612
CsiI ACCWGGT 1 cut(s) 1165
Csp6I GTAC 4 cut(s) 424, 647, 712, 961
CviQI GTAC 4 cut(s) 424, 647, 712, 961
DdeI CTNAG 6 cut(s) 72, 255, 540, 611, 638, 1000
DpnI GATC 4 cut(s) 596, 701, 809, 998
DpnII GATC 4 cut(s) 594, 699, 807, 996
EaeI YGGCCR 2 cut(s) 6, 823
Ecl136II GAGCTC 1 cut(s) 461
Eco130I CCWWGG 2 cut(s) 419, 1015
Eco24I GRGCYC 1 cut(s) 463
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 461
Eco57I CTGAAG 2 cut(s) 344, 700
Eco81I CCTNAGG 1 cut(s) 638
Eco88I CYCGRG 2 cut(s) 456, 1189
EcoICRI GAGCTC 1 cut(s) 461
EcoRI GAATTC 2 cut(s) 149, 861
EcoRII CCWGG 5 cut(s) 224, 238, 269, 1046, 1165
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 419, 1015
EcoT22I ATGCAT 2 cut(s) 549, 1134
EcoT38I GRGCYC 1 cut(s) 463
ErhI CCWWGG 2 cut(s) 419, 1015
FaqI GGGAC 2 cut(s) 280, 968
FbaI TGATCA 2 cut(s) 594, 699
FblI GTMKAC 1 cut(s) 471
FriOI GRGCYC 1 cut(s) 463
FspBI CTAG 3 cut(s) 140, 420, 974
GsaI CCCAGC 1 cut(s) 350
GsuI CTGGAG 2 cut(s) 447, 545
HaeIII GGCC 3 cut(s) 8, 825, 922
HapII CCGG 3 cut(s) 5, 628, 943
HgaI GACGC 1 cut(s) 612
HincII GTYRAC 2 cut(s) 733, 1163
HindII GTYRAC 2 cut(s) 733, 1163
HinfI GANTC 3 cut(s) 490, 655, 813
HpaII CCGG 3 cut(s) 5, 628, 943
HphI GGTGA 5 cut(s) 187, 322, 419, 626, 880
Hpy166II GTNNAC 9 cut(s) 195, 235, 415, 472, 733, 1006, 1060, 1163, 1172
Hpy188I TCNGA 6 cut(s) 73, 258, 719, 812, 996, 1067
Hpy188III TCNNGA 4 cut(s) 394, 562, 640, 834
Hpy8I GTNNAC 9 cut(s) 195, 235, 415, 472, 733, 1006, 1060, 1163, 1172
Hpy99I CGWCG 1 cut(s) 445
HpyAV CCTTC 2 cut(s) 375, 895
HpyCH4III ACNGT 4 cut(s) 43, 294, 646, 737
HpyCH4IV ACGT 2 cut(s) 305, 443
HpyCH4V TGCA 6 cut(s) 123, 547, 876, 983, 1132, 1136
HpyF10VI GCNNNNNNNGC 9 cut(s) 544, 582, 612, 803, 910, 919, 989, 1091, 1191
HpyF3I CTNAG 6 cut(s) 72, 255, 540, 611, 638, 1000
HpySE526I ACGT 2 cut(s) 305, 443
KroI GCCGGC 2 cut(s) 4, 627
KroNI GCCGGC 2 cut(s) 6, 629
Ksp22I TGATCA 2 cut(s) 594, 699
Kzo9I GATC 4 cut(s) 594, 699, 807, 996
LmnI GCTCC 4 cut(s) 143, 466, 535, 1190
LweI GCATC 2 cut(s) 534, 640
MabI ACCWGGT 1 cut(s) 1165
MaeI CTAG 3 cut(s) 140, 420, 974
MaeII ACGT 2 cut(s) 305, 443
MaeIII GTNAC 2 cut(s) 335, 779
MalI GATC 4 cut(s) 596, 701, 809, 998
MboI GATC 4 cut(s) 594, 699, 807, 996
MfeI CAATTG 1 cut(s) 85
MhlI GDGCHC 2 cut(s) 463, 1196
MluCI AATT 7 cut(s) 24, 66, 77, 85, 149, 447, 861
MlyI GAGTC 1 cut(s) 807
MnlI CCTC 9 cut(s) 58, 61, 264, 398, 508, 647, 1143, 1188, 1198
Mph1103I ATGCAT 2 cut(s) 549, 1134
MroNI GCCGGC 2 cut(s) 4, 627
MroXI GAANNNNTTC 1 cut(s) 1010
MseI TTAA 5 cut(s) 38, 213, 446, 660, 917
MslI CAYNNNNRTG 2 cut(s) 203, 590
MspA1I CMGCKG 2 cut(s) 467, 600
MspI CCGG 3 cut(s) 5, 628, 943
MspR9I CCNGG 5 cut(s) 226, 240, 271, 1048, 1167
MunI CAATTG 1 cut(s) 85
Mva1269I GAATGC 2 cut(s) 210, 547
MvaI CCWGG 5 cut(s) 226, 240, 271, 1048, 1167
MvnI CGCG 2 cut(s) 440, 606
MwoI GCNNNNNNNGC 9 cut(s) 544, 582, 612, 803, 910, 919, 989, 1091, 1191
NaeI GCCGGC 2 cut(s) 6, 629
NcoI CCATGG 1 cut(s) 1015
NdeII GATC 4 cut(s) 594, 699, 807, 996
NgoMIV GCCGGC 2 cut(s) 4, 627
NlaIV GGNNCC 4 cut(s) 244, 268, 537, 1186
NmeAIII GCCGAG 1 cut(s) 801
NmuCI GTSAC 1 cut(s) 335
NsiI ATGCAT 2 cut(s) 549, 1134
NspI RCATGY 3 cut(s) 430, 961, 1136
NspV TTCGAA 1 cut(s) 482
PaeI GCATGC 1 cut(s) 1136
PciI ACATGT 2 cut(s) 426, 957
PcsI WCGNNNNNNNCGW 1 cut(s) 437
PctI GAATGC 2 cut(s) 210, 547
PdiI GCCGGC 2 cut(s) 6, 629
PdmI GAANNNNTTC 1 cut(s) 1010
PfeI GAWTC 2 cut(s) 490, 655
PflFI GACNNNGTC 1 cut(s) 606
PflMI CCANNNNNTGG 1 cut(s) 568
Ple19I CGATCG 1 cut(s) 810
PleI GAGTC 1 cut(s) 807
PpsI GAGTC 1 cut(s) 807
PscI ACATGT 2 cut(s) 426, 957
Psp124BI GAGCTC 1 cut(s) 463
Psp1406I AACGTT 1 cut(s) 305
Psp6I CCWGG 5 cut(s) 224, 238, 269, 1046, 1165
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 5 cut(s) 224, 238, 269, 1046, 1165
PspN4I GGNNCC 4 cut(s) 244, 268, 537, 1186
PspPI GGNCC 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 606
PvuI CGATCG 1 cut(s) 810
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 4 cut(s) 425, 648, 713, 962
RsaNI GTAC 4 cut(s) 424, 647, 712, 961
RseI CAYNNNNRTG 2 cut(s) 203, 590
SacI GAGCTC 1 cut(s) 463
SaqAI TTAA 5 cut(s) 38, 213, 446, 660, 917
Sau3AI GATC 4 cut(s) 594, 699, 807, 996
Sau96I GGNCC 1 cut(s) 267
SchI GAGTC 1 cut(s) 807
ScrFI CCNGG 5 cut(s) 226, 240, 271, 1048, 1167
SduI GDGCHC 2 cut(s) 463, 1196
SexAI ACCWGGT 1 cut(s) 1165
SfaNI GCATC 2 cut(s) 534, 640
SfcI CTRYAG 1 cut(s) 841
SfuI TTCGAA 1 cut(s) 482
SinI GGWCC 1 cut(s) 267
SmiMI CAYNNNNRTG 2 cut(s) 203, 590
SmlI CTYRAG 1 cut(s) 1179
SmoI CTYRAG 1 cut(s) 1179
SphI GCATGC 1 cut(s) 1136
Sse9I AATT 7 cut(s) 24, 66, 77, 85, 149, 447, 861
SsiI CCGC 3 cut(s) 438, 600, 606
SspI AATATT 2 cut(s) 664, 950
SspMI CTAG 3 cut(s) 140, 420, 974
SstI GAGCTC 1 cut(s) 463
StyD4I CCNGG 5 cut(s) 224, 238, 269, 1046, 1165
StyI CCWWGG 2 cut(s) 419, 1015
TaaI ACNGT 4 cut(s) 43, 294, 646, 737
TaiI ACGT 2 cut(s) 308, 446
TaqI TCGA 3 cut(s) 431, 482, 859
TasI AATT 7 cut(s) 24, 66, 77, 85, 149, 447, 861
TfiI GAWTC 2 cut(s) 490, 655
Tru1I TTAA 5 cut(s) 38, 213, 446, 660, 917
Tru9I TTAA 5 cut(s) 38, 213, 446, 660, 917
TscAI CASTG 3 cut(s) 340, 990, 1008
TseFI GTSAC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 3 cut(s) 122, 746, 1117
TspRI CASTG 3 cut(s) 340, 990, 1008
Tth111I GACNNNGTC 1 cut(s) 606
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 267
XapI RAATTY 2 cut(s) 149, 861
XceI RCATGY 3 cut(s) 430, 961, 1136
XcmI CCANNNNNNNNNTGG 1 cut(s) 304
XmaJI CCTAGG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 1010
XspI CTAG 3 cut(s) 140, 420, 974
Zsp2I ATGCAT 2 cut(s) 549, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.