FvH4_3g24442

MuDR family transposase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009659
Physical Location & Seq
Reverse (-)
17506147 .. 17507664
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24442.t1

Sequence Viewer

Length: 1185 bp
ATGAGAACAGTAACCAGAAAAGTTCTTATTTTCTTGCCCACTTTCAAATTGGGAGGAATGGAGACTGCTACAATTGCAAGGTGTAGTTATCGTGCTGTAACTCTGGTCATTCCATTGTTTGAACATACAAAATATGCTGATATGCGTAATGAGATAAGTTCAAGGTTTGAGGAACTGGAAGTAGGGGGTTTTGAGCTGACATTTTCTATTCCTGAGCATCCTAATATCATGTTGGAATCTGATATGGACATGCACATGATGCTCATGTTTTTATCCATGGTGAAGAGTCGTTTTGTTGATATATTGGTAAAGGATCTTGAGAGTTCGAAGAAGGAGGATGTTGCTCGTGCAGTGTCCGGTCAAGCAGCAGATAATCCTGCATTCTCGGATCATGCAGCTTCTGATCATTCGGCGTCTAATTGTGCTGCTTTTAATAATACAGCATCTGACCTTGCAGTGCCTAATCATGAAATGTCTAATGATGCAGCATCTAACCATGCAGCCTCTGATCCTGCACTGCCTAAACATGCAACATCTAATATACAGTCACCTATTCCTGCAATATCTGATTTTGCAGCGTCTAATCATGTATCATCATCGTCATGCATGGTAGAAAGTAAATTCATTGACGAAAATGAATATTTGGGGAACTCTGGAGTACATGAAGGCAATGCATATTTATCTCAAGGTTGGAAGGAATATATTAATCATGTAGGACAGAAGTTTGAGGGTGGTGCAGCTGAATTTCGTAACAAGTTGTGTAAGTATGCCTTTGAGATGGGATTCCGTATTCGTTATTTAAGAAATTATAGGACTCACGTTATTGCTGAATGTCGGAAGAAGCTATCAGATGGATGTAGTTGGCGTATACATGCCTGCAAGTGTCAAGTGAATGCGTTTTTCTACCTTAGGACTTTAAATAATGTTCACACTTGCGAGAGAGTTATCTTCCCTGAACAGGCAAGTAAGTTGACCACTTCAATGATTATATCTTCTGTTCTGGTGGATCAAATTCGTGAGAAACCATATATTAAACCAACTGATATTGTTAAGGATTTCAAGCAAAAATATGGTCTTGATGTCTCTTATCGTAAGGCCTGGTTAGCAAAAGAACTGGCTAAGAGAACAGTTCATGGTGATGAATCCCTCTCATATCGTCAGTTAGTTTGGTTTGATGACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

395

Amino Acids

44.5

Weight (kDa)

6.74

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBD_Tnp_Mut PF03108 237 - 296 4.3e-09 MuDR family transposase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 868
AclWI GGATC 4 cut(s) 321, 396, 503, 1014
AcsI RAATTY 3 cut(s) 620, 743, 1011
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 1 cut(s) 660
AfiI CCNNNNNNNGG 1 cut(s) 958
AgsI TTSAA 5 cut(s) 46, 122, 162, 981, 1060
AjnI CCWGG 1 cut(s) 1097
AluBI AGCT 4 cut(s) 196, 398, 740, 844
AluI AGCT 4 cut(s) 196, 398, 740, 844
Alw26I GTCTC 2 cut(s) 56, 1087
AlwI GGATC 4 cut(s) 321, 396, 503, 1014
AlwNI CAGNNNCTG 3 cut(s) 401, 446, 506
AoxI GGCC 1 cut(s) 1095
ApeKI GCWGC 7 cut(s) 365, 395, 425, 485, 500, 575, 737
ApoI RAATTY 3 cut(s) 620, 743, 1011
ArsI GACNNNNNNTTYG 2 cut(s) 1057, 1089
AseI ATTAAT 1 cut(s) 705
AsuHPI GGTGA 3 cut(s) 292, 540, 1148
AsuII TTCGAA 1 cut(s) 326
AxyI CCTNAGG 1 cut(s) 908
BauI CACGAG 1 cut(s) 345
BbvI GCAGC 7 cut(s) 377, 407, 412, 497, 512, 587, 749
BccI CCATC 2 cut(s) 772, 845
BciT130I CCWGG 1 cut(s) 1099
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 56, 1087
BisI GCNGC 7 cut(s) 366, 396, 426, 486, 501, 576, 738
BlsI GCNGC 7 cut(s) 367, 397, 427, 487, 502, 577, 739
Bme1390I CCNGG 1 cut(s) 1099
BmrFI CCNGG 1 cut(s) 1099
BmsI GCATC 5 cut(s) 226, 249, 452, 472, 497
BpmI CTGGAG 1 cut(s) 675
Bpu10I CCTNAGC 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 326
BpuEI CTTGAG 2 cut(s) 338, 669
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 1 cut(s) 958
Bse1I ACTGG 2 cut(s) 180, 1119
Bse21I CCTNAGG 1 cut(s) 908
Bse3DI GCAATG 1 cut(s) 676
BseBI CCWGG 1 cut(s) 1099
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 3 cut(s) 217, 343, 860
BseLI CCNNNNNNNGG 1 cut(s) 958
BseMI GCAATG 1 cut(s) 676
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 180, 1119
BseXI GCAGC 7 cut(s) 377, 407, 412, 497, 512, 587, 749
BsgI GTGCAG 3 cut(s) 369, 498, 756
BshFI GGCC 1 cut(s) 1097
BsiSI CCGG 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 958
BsmAI GTCTC 2 cut(s) 56, 1087
BsmI GAATGC 2 cut(s) 380, 898
BsnI GGCC 1 cut(s) 1097
Bsp119I TTCGAA 1 cut(s) 326
Bsp143I GATC 5 cut(s) 313, 388, 403, 508, 1006
Bsp19I CCATGG 1 cut(s) 276
BspANI GGCC 1 cut(s) 1097
BspCNI CTCAG 1 cut(s) 205
BspHI TCATGA 1 cut(s) 466
BspPI GGATC 4 cut(s) 321, 396, 503, 1014
BspT104I TTCGAA 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 676
BsrI ACTGG 2 cut(s) 180, 1119
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 5 cut(s) 313, 388, 403, 508, 1006
BssNAI GTATAC 1 cut(s) 869
BssNI GRCGYC 1 cut(s) 413
BssSI CACGAG 1 cut(s) 345
BssT1I CCWWGG 1 cut(s) 276
Bst1107I GTATAC 1 cut(s) 869
Bst2BI CACGAG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 1099
Bst4CI ACNGT 3 cut(s) 10, 546, 1129
Bst6I CTCTTC 1 cut(s) 278
BstACI GRCGYC 1 cut(s) 413
BstAPI GCANNNNNTGC 1 cut(s) 259
BstBI TTCGAA 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 877
BstDEI CTNAG 3 cut(s) 213, 908, 1119
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 3 cut(s) 217, 343, 860
BstKTI GATC 5 cut(s) 316, 391, 406, 511, 1009
BstMAI GTCTC 2 cut(s) 56, 1087
BstMBI GATC 5 cut(s) 313, 388, 403, 508, 1006
BstMWI GCNNNNNNNGC 3 cut(s) 74, 259, 1103
BstNI CCWGG 1 cut(s) 1099
BstNSI RCATGY 3 cut(s) 253, 530, 875
BstSCI CCNGG 1 cut(s) 1097
BstV1I GCAGC 7 cut(s) 377, 407, 412, 497, 512, 587, 749
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BstZ17I GTATAC 1 cut(s) 869
Bsu36I CCTNAGG 1 cut(s) 908
BsuRI GGCC 1 cut(s) 1097
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 3 cut(s) 217, 343, 860
BtsI GCAGTG 3 cut(s) 357, 462, 515
BtsIMutI CAGTG 3 cut(s) 357, 462, 515
Cac8I GCNNGC 1 cut(s) 877
CaiI CAGNNNCTG 3 cut(s) 401, 446, 506
CciI TCATGA 1 cut(s) 466
CseI GACGC 2 cut(s) 402, 567
Csp6I GTAC 1 cut(s) 659
CviJI RGCY 7 cut(s) 196, 398, 503, 740, 844, 1097, 1118
CviKI_1 RGCY 7 cut(s) 196, 398, 503, 740, 844, 1097, 1118
CviQI GTAC 1 cut(s) 659
DdeI CTNAG 3 cut(s) 213, 908, 1119
DpnI GATC 5 cut(s) 315, 390, 405, 510, 1008
DpnII GATC 5 cut(s) 313, 388, 403, 508, 1006
DraI TTTAAA 1 cut(s) 918
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 1097
Eco81I CCTNAGG 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 1097
EcoT14I CCWWGG 1 cut(s) 276
EcoT22I ATGCAT 2 cut(s) 608, 676
ErhI CCWWGG 1 cut(s) 276
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 7 cut(s) 366, 396, 426, 486, 501, 576, 738
FokI GGATG 3 cut(s) 204, 350, 867
Fsp4HI GCNGC 7 cut(s) 366, 396, 426, 486, 501, 576, 738
GluI GCNGC 7 cut(s) 366, 396, 426, 486, 501, 576, 738
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 1 cut(s) 1097
HapII CCGG 1 cut(s) 357
HgaI GACGC 2 cut(s) 402, 567
Hin1I GRCGYC 1 cut(s) 413
HincII GTYRAC 1 cut(s) 972
HindII GTYRAC 1 cut(s) 972
HinfI GANTC 5 cut(s) 236, 286, 783, 814, 1142
HpaII CCGG 1 cut(s) 357
HphI GGTGA 3 cut(s) 292, 540, 1148
Hpy166II GTNNAC 3 cut(s) 869, 928, 972
Hpy188I TCNGA 8 cut(s) 241, 388, 403, 448, 508, 568, 837, 850
Hpy188III TCNNGA 6 cut(s) 212, 317, 467, 654, 1016, 1076
Hpy8I GTNNAC 3 cut(s) 869, 928, 972
HpyAV CCTTC 3 cut(s) 325, 659, 688
HpyCH4III ACNGT 3 cut(s) 10, 546, 1129
HpyCH4IV ACGT 1 cut(s) 819
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 259, 1103
HpyF3I CTNAG 3 cut(s) 213, 908, 1119
HpySE526I ACGT 1 cut(s) 819
Hsp92I GRCGYC 1 cut(s) 413
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 5 cut(s) 313, 388, 403, 508, 1006
Lsp1109I GCAGC 7 cut(s) 377, 407, 412, 497, 512, 587, 749
LweI GCATC 5 cut(s) 226, 249, 452, 472, 497
MaeII ACGT 1 cut(s) 819
MaeIII GTNAC 4 cut(s) 10, 97, 546, 749
MalI GATC 5 cut(s) 315, 390, 405, 510, 1008
MboI GATC 5 cut(s) 313, 388, 403, 508, 1006
MboII GAAGA 5 cut(s) 295, 340, 850, 940, 984
MfeI CAATTG 1 cut(s) 72
MflI RGATCY 1 cut(s) 313
MluCI AATT 7 cut(s) 47, 72, 418, 620, 743, 805, 1011
MlyI GAGTC 2 cut(s) 295, 808
MmeI TCCRAC 3 cut(s) 213, 671, 815
MnlI CCTC 6 cut(s) 47, 163, 328, 514, 721, 1157
Mph1103I ATGCAT 2 cut(s) 608, 676
MseI TTAA 6 cut(s) 432, 705, 800, 917, 1032, 1050
MslI CAYNNNNRTG 4 cut(s) 254, 601, 980, 1137
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 1099
MunI CAATTG 1 cut(s) 72
Mva1269I GAATGC 2 cut(s) 380, 898
MvaI CCWGG 1 cut(s) 1099
MwoI GCNNNNNNNGC 3 cut(s) 74, 259, 1103
NcoI CCATGG 1 cut(s) 276
NdeII GATC 5 cut(s) 313, 388, 403, 508, 1006
NmuCI GTSAC 1 cut(s) 546
NsiI ATGCAT 2 cut(s) 608, 676
NspI RCATGY 3 cut(s) 253, 530, 875
NspV TTCGAA 1 cut(s) 326
PagI TCATGA 1 cut(s) 466
PceI AGGCCT 1 cut(s) 1097
PctI GAATGC 2 cut(s) 380, 898
PfeI GAWTC 3 cut(s) 236, 783, 1142
PkrI GCNGC 7 cut(s) 367, 397, 427, 487, 502, 577, 739
PleI GAGTC 2 cut(s) 294, 808
PpsI GAGTC 2 cut(s) 294, 808
PshBI ATTAAT 1 cut(s) 705
Psp6I CCWGG 1 cut(s) 1097
PspGI CCWGG 1 cut(s) 1097
PstNI CAGNNNCTG 3 cut(s) 401, 446, 506
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 1 cut(s) 660
RsaNI GTAC 1 cut(s) 659
RseI CAYNNNNRTG 4 cut(s) 254, 601, 980, 1137
SaqAI TTAA 6 cut(s) 432, 705, 800, 917, 1032, 1050
SatI GCNGC 7 cut(s) 366, 396, 426, 486, 501, 576, 738
Sau3AI GATC 5 cut(s) 313, 388, 403, 508, 1006
SchI GAGTC 2 cut(s) 295, 808
ScrFI CCNGG 1 cut(s) 1099
SfaNI GCATC 5 cut(s) 226, 249, 452, 472, 497
SfuI TTCGAA 1 cut(s) 326
SmiMI CAYNNNNRTG 4 cut(s) 254, 601, 980, 1137
SmlI CTYRAG 2 cut(s) 317, 684
SmoI CTYRAG 2 cut(s) 317, 684
Sse9I AATT 7 cut(s) 47, 72, 418, 620, 743, 805, 1011
SseBI AGGCCT 1 cut(s) 1097
SspI AATATT 1 cut(s) 641
StuI AGGCCT 1 cut(s) 1097
StyD4I CCNGG 1 cut(s) 1097
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 3 cut(s) 10, 546, 1129
TaiI ACGT 1 cut(s) 822
TaqI TCGA 1 cut(s) 326
TasI AATT 7 cut(s) 47, 72, 418, 620, 743, 805, 1011
TatI WGTACW 1 cut(s) 658
TfiI GAWTC 3 cut(s) 236, 783, 1142
Tru1I TTAA 6 cut(s) 432, 705, 800, 917, 1032, 1050
Tru9I TTAA 6 cut(s) 432, 705, 800, 917, 1032, 1050
TscAI CASTG 3 cut(s) 357, 462, 522
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 7 cut(s) 365, 395, 425, 485, 500, 575, 737
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 6 cut(s) 483, 613, 651, 678, 1121, 1155
TspGWI ACGGA 1 cut(s) 776
TspRI CASTG 3 cut(s) 357, 462, 522
VspI ATTAAT 1 cut(s) 705
XapI RAATTY 3 cut(s) 620, 743, 1011
XceI RCATGY 3 cut(s) 253, 530, 875
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 868
Zsp2I ATGCAT 2 cut(s) 608, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.