Rh5DG310800

plant mutator transposase zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0009659
Physical Location & Seq
Reverse (-)
39661211 .. 39664856
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG310800.1

Sequence Viewer

Length: 687 bp
ATGGAACCCAAACTCGAATCAGGACGACTGGATAGTGCTACACTAGCAAAGTGCACTTATCGCTCAGTAGCATTCCTAACTGCATTGTATGAAAATAGTAAGTATGCGGACATTTGTGATGAGATTTGTTCAAGGTTTAAGGTACTGGACATAGGGAGCTTTGAGCTGACATATGCTATCCCTGATCACCCTAATTGTTTGCTGCAATCGGATATGGATGTGCGCATGATGTTGATGTGTCTAGAAATGTTGAATATTAGGATTGTTGATATATTAGTGAAGGATCGTGTGAATTCCAATATTGAAGGCGCTGGCCAAAATCATGTAGAGTCTGCAGGTTCTAATAATGTGCCTATTCATACAACACCTATGTCACGCACAGTAGAAACTCACAGTGCCACTGGTGAGAATGAACACTTGGGGAATTCAACATCACATGAAGATAATGCATATTTGTCTCAAGGTGCAACTGGGAAGGATGGAAATCAAGGATATTTTCCTTTTGCGTTTGCGATCATGGACTCGGAAAGCGATGAAAACTGGAGCTGGTTCTTTGATAATTTAGCAAAAGTTTTGACTCCGGAAGGCAGGACAATTACATTTGTATCTGATCGTAACAAAGGTTTGATTGAAGGGATTTGCTCCCAGGAGGATCTCCCAACCCCGGAGGATAAGTCGGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

228

Amino Acids

25.35

Weight (kDa)

4.55

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MULE PF10551 156 - 215 2.6e-07 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 224
Acc36I ACCTGC 1 cut(s) 326
AccIII TCCGGA 1 cut(s) 580
AciI CCGC 1 cut(s) 107
AclWI GGATC 2 cut(s) 291, 660
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 2 cut(s) 292, 424
AfaI GTAC 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 664
AgsI TTSAA 5 cut(s) 132, 253, 305, 429, 632
AjnI CCWGG 1 cut(s) 645
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AluBI AGCT 3 cut(s) 159, 166, 546
AluI AGCT 3 cut(s) 159, 166, 546
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 1 cut(s) 462
Alw44I GTGCAC 1 cut(s) 52
AlwI GGATC 2 cut(s) 291, 660
Aor13HI TCCGGA 1 cut(s) 580
AoxI GGCC 1 cut(s) 313
ApaLI GTGCAC 1 cut(s) 52
ApeKI GCWGC 1 cut(s) 202
ApoI RAATTY 2 cut(s) 292, 424
AspLEI GCGC 2 cut(s) 225, 311
AsuC2I CCSGG 1 cut(s) 665
AsuHPI GGTGA 2 cut(s) 179, 416
BaeGI GKGCMC 1 cut(s) 56
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BalI TGGCCA 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 189
BccI CCATC 1 cut(s) 473
BciT130I CCWGG 1 cut(s) 647
BclI TGATCA 1 cut(s) 184
BcnI CCSGG 1 cut(s) 665
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 2 cut(s) 44, 242
BfmI CTRYAG 1 cut(s) 333
BfoI RGCGCY 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 326
BisI GCNGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 204
Bme1390I CCNGG 2 cut(s) 647, 665
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 647, 665
BmrI ACTGGG 1 cut(s) 480
BmuI ACTGGG 1 cut(s) 480
BpmI CTGGAG 1 cut(s) 562
BpuEI CTTGAG 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 2 cut(s) 645, 663
BsaWI WCCGGW 1 cut(s) 580
BsaXI ACNNNNNCTCC 1 cut(s) 659
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse1I ACTGG 5 cut(s) 33, 150, 406, 475, 545
Bse8I GATNNNNATC 1 cut(s) 483
BseAI TCCGGA 1 cut(s) 580
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 2 cut(s) 645, 663
BseGI GGATG 2 cut(s) 223, 484
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 5 cut(s) 33, 150, 406, 475, 545
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 1 cut(s) 189
BshFI GGCC 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 581, 665
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 1 cut(s) 462
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 56
Bsp13I TCCGGA 1 cut(s) 580
Bsp143I GATC 5 cut(s) 184, 283, 513, 610, 652
BspACI CCGC 1 cut(s) 107
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 1 cut(s) 77
BspEI TCCGGA 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 337
BspMI ACCTGC 1 cut(s) 326
BspPI GGATC 2 cut(s) 291, 660
BsrI ACTGG 5 cut(s) 33, 150, 406, 475, 545
BssECI CCNNGG 2 cut(s) 645, 663
BssMI GATC 5 cut(s) 184, 283, 513, 610, 652
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 2 cut(s) 382, 395
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 64
BstF5I GGATG 2 cut(s) 223, 484
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 225, 311
BstKTI GATC 5 cut(s) 187, 286, 516, 613, 655
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 5 cut(s) 184, 283, 513, 610, 652
BstMWI GCNNNNNNNGC 2 cut(s) 44, 60
BstNI CCWGG 1 cut(s) 647
BstSCI CCNGG 2 cut(s) 645, 663
BstSFI CTRYAG 1 cut(s) 333
BstSLI GKGCMC 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 652
BstYI RGATCY 1 cut(s) 652
BsuRI GGCC 1 cut(s) 315
BtgZI GCGATG 1 cut(s) 546
BtsCI GGATG 2 cut(s) 223, 484
BtsIMutI CAGTG 2 cut(s) 399, 400
BveI ACCTGC 1 cut(s) 326
Cac8I GCNNGC 1 cut(s) 313
CfoI GCGC 2 cut(s) 225, 311
Csp6I GTAC 1 cut(s) 143
CviAII CATG 4 cut(s) 226, 323, 437, 517
CviJI RGCY 4 cut(s) 159, 166, 315, 546
CviKI_1 RGCY 4 cut(s) 159, 166, 315, 546
CviQI GTAC 1 cut(s) 143
DdeI CTNAG 1 cut(s) 64
DpnI GATC 5 cut(s) 186, 285, 515, 612, 654
DpnII GATC 5 cut(s) 184, 283, 513, 610, 652
EaeI YGGCCR 1 cut(s) 313
EcoRI GAATTC 2 cut(s) 292, 424
EcoRII CCWGG 1 cut(s) 645
EcoT22I ATGCAT 1 cut(s) 451
FaeI CATG 4 cut(s) 229, 326, 440, 520
FatI CATG 4 cut(s) 225, 322, 436, 516
FauNDI CATATG 1 cut(s) 172
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 203
FokI GGATG 2 cut(s) 230, 491
Fsp4HI GCNGC 1 cut(s) 203
FspAI RTGCGCAY 1 cut(s) 224
FspBI CTAG 2 cut(s) 44, 242
FspI TGCGCA 1 cut(s) 224
GlaI GCGC 2 cut(s) 224, 310
GluI GCNGC 1 cut(s) 203
GsuI CTGGAG 1 cut(s) 562
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 1 cut(s) 315
HapII CCGG 2 cut(s) 581, 665
HhaI GCGC 2 cut(s) 225, 311
Hin1II CATG 4 cut(s) 229, 326, 440, 520
Hin6I GCGC 2 cut(s) 223, 309
HinP1I GCGC 2 cut(s) 223, 309
HinfI GANTC 4 cut(s) 17, 329, 521, 577
HpaII CCGG 2 cut(s) 581, 665
HphI GGTGA 2 cut(s) 179, 416
Hpy166II GTNNAC 1 cut(s) 54
Hpy188I TCNGA 4 cut(s) 211, 526, 610, 679
Hpy188III TCNNGA 3 cut(s) 21, 242, 581
Hpy8I GTNNAC 1 cut(s) 54
HpyAV CCTTC 5 cut(s) 274, 299, 469, 578, 626
HpyCH4III ACNGT 2 cut(s) 382, 395
HpyCH4V TGCA 6 cut(s) 54, 83, 205, 335, 449, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 60
HpyF3I CTNAG 1 cut(s) 64
Hsp92II CATG 4 cut(s) 229, 326, 440, 520
HspAI GCGC 2 cut(s) 223, 309
Kpn2I TCCGGA 1 cut(s) 580
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 5 cut(s) 184, 283, 513, 610, 652
LmnI GCTCC 3 cut(s) 156, 543, 647
Lsp1109I GCAGC 1 cut(s) 189
MaeI CTAG 2 cut(s) 44, 242
MaeIII GTNAC 2 cut(s) 372, 614
MalI GATC 5 cut(s) 186, 285, 515, 612, 654
MboI GATC 5 cut(s) 184, 283, 513, 610, 652
MboII GAAGA 1 cut(s) 452
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 1 cut(s) 56
MlsI TGGCCA 1 cut(s) 315
MluCI AATT 5 cut(s) 193, 292, 424, 559, 594
MluNI TGGCCA 1 cut(s) 315
MlyI GAGTC 3 cut(s) 338, 515, 571
MmeI TCCRAC 1 cut(s) 657
MnlI CCTC 2 cut(s) 643, 661
Mox20I TGGCCA 1 cut(s) 315
Mph1103I ATGCAT 1 cut(s) 451
MroI TCCGGA 1 cut(s) 580
MscI TGGCCA 1 cut(s) 315
MseI TTAA 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 315
MspI CCGG 2 cut(s) 581, 665
MspR9I CCNGG 2 cut(s) 647, 665
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 2 cut(s) 44, 60
NciI CCSGG 1 cut(s) 665
NdeI CATATG 1 cut(s) 172
NdeII GATC 5 cut(s) 184, 283, 513, 610, 652
NlaIII CATG 4 cut(s) 229, 326, 440, 520
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 372
NsbI TGCGCA 1 cut(s) 224
NsiI ATGCAT 1 cut(s) 451
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 204
PleI GAGTC 3 cut(s) 337, 515, 571
PpsI GAGTC 3 cut(s) 337, 515, 571
Psp6I CCWGG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 6
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 652
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 1 cut(s) 203
Sau3AI GATC 5 cut(s) 184, 283, 513, 610, 652
SchI GAGTC 3 cut(s) 338, 515, 571
ScrFI CCNGG 2 cut(s) 647, 665
SduI GDGCHC 1 cut(s) 56
SetI ASST 9 cut(s) 137, 144, 161, 168, 340, 370, 466, 548, 625
SfcI CTRYAG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 5 cut(s) 193, 292, 424, 559, 594
SsiI CCGC 1 cut(s) 107
SspI AATATT 2 cut(s) 256, 301
SspMI CTAG 2 cut(s) 44, 242
StyD4I CCNGG 2 cut(s) 645, 663
TaaI ACNGT 2 cut(s) 382, 395
TaqI TCGA 1 cut(s) 15
TasI AATT 5 cut(s) 193, 292, 424, 559, 594
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 2 cut(s) 400, 406
TseFI GTSAC 1 cut(s) 372
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 372
TspDTI ATGAA 5 cut(s) 105, 347, 426, 453, 549
TspRI CASTG 2 cut(s) 400, 406
VneI GTGCAC 1 cut(s) 52
XapI RAATTY 2 cut(s) 292, 424
XbaI TCTAGA 1 cut(s) 241
XspI CTAG 2 cut(s) 44, 242
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.