Rh7CG227000

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0009659
Physical Location & Seq
Reverse (-)
21103317 .. 21133399
30083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG227000.1

Sequence Viewer

Length: 399 bp
ATGTATTCAGAGATGACAGCTAGGTGCACTTACATGTCAGAGACTGTGATGGTCTTCCTTGATGGTACGACAAGGTTCTCCGATTTTTTCAATGAAATTTGCATGCGGTTTAAGGGTTTGAAGCCAGGCTTGTTTGAGTTGAAGTATTCCCTTCCGGATTACCCTTCTTGTGCTCTCGATTCTGATTTAGATATGAGGTTGATGTTTAGGTCTTTAGCTGAGTTTAAGAGAACTTCAGTTGACATTTTGGTGAGGATTTGTGATTTTGGTGCAAATTGTGTTGCTGATGGAGTTCAATGTTCTGCCGCTGCTGCTGCTTCTTCTTGTGTGTCAGTGGTATCGACGGTGATTGAGGAGCCTGATTATTTGGTGCTCAAACACTCAGCTGCAGGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

132

Amino Acids

14.66

Weight (kDa)

4.75

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 5 - 85 2.6e-06 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 154
AciI CCGC 2 cut(s) 106, 306
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 1 cut(s) 67
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 4 cut(s) 91, 121, 142, 296
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 3 cut(s) 20, 218, 386
AluI AGCT 3 cut(s) 20, 218, 386
Alw21I GWGCWC 3 cut(s) 29, 175, 375
Alw26I GTCTC 1 cut(s) 35
Alw44I GTGCAC 1 cut(s) 25
AlwNI CAGNNNCTG 1 cut(s) 44
Aor13HI TCCGGA 1 cut(s) 154
ApaLI GTGCAC 1 cut(s) 25
ApeKI GCWGC 4 cut(s) 308, 311, 314, 386
ApoI RAATTY 1 cut(s) 96
AsuHPI GGTGA 2 cut(s) 262, 358
BaeGI GKGCMC 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 3 cut(s) 29, 175, 375
BbvI GCAGC 4 cut(s) 295, 298, 301, 373
BccI CCATC 3 cut(s) 43, 56, 281
BciT130I CCWGG 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 1 cut(s) 21
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 5 cut(s) 306, 309, 312, 315, 387
BlsI GCNGC 5 cut(s) 307, 310, 313, 316, 388
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 126
BpiI GAAGAC 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 154
BseAI TCCGGA 1 cut(s) 154
BseBI CCWGG 1 cut(s) 126
BseMII CTCAG 2 cut(s) 210, 396
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 4 cut(s) 295, 298, 301, 373
BsiHKAI GWGCWC 3 cut(s) 29, 175, 375
BsiSI CCGG 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 35
Bsp1286I GDGCHC 3 cut(s) 29, 175, 375
Bsp13I TCCGGA 1 cut(s) 154
BspACI CCGC 2 cut(s) 106, 306
BspCNI CTCAG 2 cut(s) 211, 395
BspEI TCCGGA 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 357
BspMAI CTGCAG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 46, 346
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 2 cut(s) 219, 382
BstMAI GTCTC 1 cut(s) 35
BstMWI GCNNNNNNNGC 2 cut(s) 311, 314
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 2 cut(s) 37, 106
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 29
BstV1I GCAGC 4 cut(s) 295, 298, 301, 373
BstV2I GAAGAC 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 339
Cac8I GCNNGC 1 cut(s) 104
CaiI CAGNNNCTG 1 cut(s) 44
Csp6I GTAC 1 cut(s) 66
CviAII CATG 2 cut(s) 34, 103
CviJI RGCY 6 cut(s) 20, 124, 129, 218, 358, 386
CviKI_1 RGCY 6 cut(s) 20, 124, 129, 218, 358, 386
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 2 cut(s) 219, 382
Eco57I CTGAAG 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 124
FaeI CATG 2 cut(s) 37, 106
FaiI YATR 3 cut(s) 35, 104, 194
FalI AAGNNNNNCTT 2 cut(s) 113, 145
FatI CATG 2 cut(s) 33, 102
Fnu4HI GCNGC 5 cut(s) 306, 309, 312, 315, 387
Fsp4HI GCNGC 5 cut(s) 306, 309, 312, 315, 387
FspBI CTAG 1 cut(s) 21
GluI GCNGC 5 cut(s) 306, 309, 312, 315, 387
HapII CCGG 1 cut(s) 155
Hin1II CATG 2 cut(s) 37, 106
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 155
HphI GGTGA 2 cut(s) 262, 358
Hpy166II GTNNAC 2 cut(s) 27, 241
Hpy188I TCNGA 4 cut(s) 10, 40, 82, 184
Hpy188III TCNNGA 2 cut(s) 155, 176
Hpy8I GTNNAC 2 cut(s) 27, 241
Hpy99I CGWCG 1 cut(s) 346
HpyAV CCTTC 2 cut(s) 161, 174
HpyCH4III ACNGT 2 cut(s) 46, 346
HpyCH4V TGCA 4 cut(s) 27, 102, 272, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 314
HpyF3I CTNAG 2 cut(s) 219, 382
Hsp92II CATG 2 cut(s) 37, 106
Kpn2I TCCGGA 1 cut(s) 154
LmnI GCTCC 1 cut(s) 355
LpnPI CCDG 5 cut(s) 111, 138, 168, 372, 375
Lsp1109I GCAGC 4 cut(s) 295, 298, 301, 373
MaeI CTAG 1 cut(s) 21
MboII GAAGA 2 cut(s) 46, 312
MhlI GDGCHC 3 cut(s) 29, 175, 375
MluCI AATT 2 cut(s) 96, 274
MnlI CCTC 3 cut(s) 189, 246, 346
MroI TCCGGA 1 cut(s) 154
MseI TTAA 2 cut(s) 111, 225
MslI CAYNNNNRTG 2 cut(s) 32, 248
MspA1I CMGCKG 2 cut(s) 308, 386
MspI CCGG 1 cut(s) 155
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 311, 314
NlaIII CATG 2 cut(s) 37, 106
NlaIV GGNNCC 1 cut(s) 357
NspI RCATGY 2 cut(s) 37, 106
PaeI GCATGC 1 cut(s) 106
PciI ACATGT 1 cut(s) 33
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 5 cut(s) 307, 310, 313, 316, 388
PscI ACATGT 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 2 cut(s) 32, 248
SaqAI TTAA 2 cut(s) 111, 225
SatI GCNGC 5 cut(s) 306, 309, 312, 315, 387
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 3 cut(s) 29, 175, 375
SetI ASST 7 cut(s) 22, 26, 77, 200, 212, 220, 388
SfcI CTRYAG 1 cut(s) 387
SmiMI CAYNNNNRTG 2 cut(s) 32, 248
SphI GCATGC 1 cut(s) 106
Sse9I AATT 2 cut(s) 96, 274
SsiI CCGC 2 cut(s) 106, 306
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 2 cut(s) 46, 346
TaqI TCGA 2 cut(s) 177, 341
TasI AATT 2 cut(s) 96, 274
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 2 cut(s) 111, 225
Tru9I TTAA 2 cut(s) 111, 225
TscAI CASTG 1 cut(s) 339
TseI GCWGC 4 cut(s) 308, 311, 314, 386
TspDTI ATGAA 1 cut(s) 108
TspRI CASTG 1 cut(s) 339
VneI GTGCAC 1 cut(s) 25
XapI RAATTY 1 cut(s) 96
XceI RCATGY 2 cut(s) 37, 106
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.