FvH4_3g25550

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005245
Physical Location & Seq
Forward (+)
18448216 .. 18456009
7794 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25550.t5

Sequence Viewer

Length: 1296 bp
ATGAGGCCGATACGTCTTCCAGAGCCGCCCAGCCCCAAATTGGGGACGCCGGAAATCTTTGAAGGCGGTGTCTCCGGCGTCGTTCGTCGCGCTGTCGTAATTGGCAACGGCTTCCCTGGTTCTGAGAATCAGAGCATCGGCTTGGTTCGCGCTCTCGGCCTCGCTGATAAGCACGTCTTGTATCGAGTTACAAGGCCAAAAGGAGGTATAAATGAGTGGCTTCACTGGCTCCCGGTTTCTGTTCACAAGAAATTGGATTGTGTCATGAGGAAAATGTTTATTTTACTAATATATCGGGGAAAGAGAGTTGGGTCTCTACCTACAGAAAATGGTGGCGGTCTTGGTTTGAAATGTGTCTTAGAAGCTGATGTCCAGGAGATTGTAACCATGGCTCGTCAGACTTATGAAACGGATGGACCCATACTGGTGGTAGCTTGTGGCAGGGATACCATTTCCGTTGCAAGTTCTATTAGACGACTAGCATCTGAAAACGTCTTTGTTGTTCAGATACTACATCCCAGGTTGCATCTCAATAGATACGACTTGGTTATTACCCCTAAACACGATTACTATCCTTTGACTCCTGAAGCACAGAAGCAGGTTCCTCAATTTCTACGTGGGTGGATAACTCCTCGTGAACCTCCTGACAGGCATGTGGTCCTCACTATTGGAGCTTTGCACCAAATTGATGCTAATTCCCTTCGTAACGCTGCCAGTCAATGGCGTGATGAATTTGCAGCTCTACCAAAGCCCTTACTCGTGGTGAACATTGGTGGTCCTACAAGCAATTGTCGTTATGGTGTGGACCTTGCAAAGCAATTGACAACCTATCTGCTTAGTGTTCTTGTAAGCTGTGGAAGCATCAGAATATGTTTCTCTAGTAGAACACCTGAAAAGGTATGCAGTGTTATAACAAAGGAGCTTGGAGGTAATCCTAAAGTTTACATTTGGGATGGTGAAGAGCCAAACCCACATATGGGCCATCTGGCGTGGGCCGATGCGTTTGTTGTTACTGCAGATTCTGTTAGCATGATAAGTGAGGTTTGCAGCACCGGGATTTTAGTTGTGTCCGCCTGTATTACCAGGAAGCCTGTTTATCTAACGGGATCTGAGCATTGTAAATGGAAGCTTTGCGATTTCCATAAATCACTCAAGGAGCGAGGAATGGTTCGACCATTTACAGGCTTGGAGAATATATCAGAAAGCTGGAGTTATCCACCGCTCAATGAAACTGCGGAAGCAGCCAATCGAGTGCATGAAGCCCTTGCTGAGCGGGGATGGAGATTGCGAGCATAG

Protein Analysis

432

Amino Acids

47.77

Weight (kDa)

9.05

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 39 - 321 8.8e-64 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 911
Acc36I ACCTGC 1 cut(s) 589
AccB7I CCANNNNNTGG 1 cut(s) 720
AccBSI CCGCTC 2 cut(s) 1222, 1273
AccII CGCG 2 cut(s) 90, 150
AciI CCGC 7 cut(s) 26, 66, 336, 1071, 1220, 1235, 1273
AclWI GGATC 1 cut(s) 1114
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 1 cut(s) 606
AcyI GRCGYC 2 cut(s) 47, 78
AfiI CCNNNNNNNGG 8 cut(s) 40, 41, 42, 203, 720, 976, 977, 1181
AgsI TTSAA 2 cut(s) 62, 349
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 4 cut(s) 115, 372, 518, 1082
AluBI AGCT 8 cut(s) 365, 434, 674, 740, 852, 922, 1129, 1206
AluI AGCT 8 cut(s) 365, 434, 674, 740, 852, 922, 1129, 1206
Alw26I GTCTC 2 cut(s) 76, 318
AlwI GGATC 1 cut(s) 1114
AlwNI CAGNNNCTG 1 cut(s) 1022
AoxI GGCC 5 cut(s) 5, 157, 194, 979, 993
ApeKI GCWGC 4 cut(s) 710, 737, 1047, 1241
ApoI RAATTY 1 cut(s) 731
AspLEI GCGC 2 cut(s) 92, 152
AspS9I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
AsuC2I CCSGG 2 cut(s) 233, 1054
AsuHPI GGTGA 2 cut(s) 775, 968
AvaII GGWCC 4 cut(s) 416, 658, 776, 805
BauI CACGAG 2 cut(s) 633, 758
BbsI GAAGAC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 697, 749, 1059, 1253
BccI CCATC 4 cut(s) 407, 947, 990, 1272
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 4 cut(s) 117, 374, 520, 1084
BciVI GTATCC 1 cut(s) 439
BcnI CCSGG 2 cut(s) 233, 1054
BcoDI GTCTC 2 cut(s) 76, 318
BfaI CTAG 2 cut(s) 479, 879
BfmI CTRYAG 2 cut(s) 321, 1014
BfuAI ACCTGC 1 cut(s) 589
BfuI GTATCC 1 cut(s) 439
BisI GCNGC 5 cut(s) 26, 711, 738, 1048, 1242
BlpI GCTNAGC 1 cut(s) 1269
BlsI GCNGC 5 cut(s) 27, 712, 739, 1049, 1243
Bme1390I CCNGG 6 cut(s) 117, 233, 374, 520, 1054, 1084
Bme18I GGWCC 4 cut(s) 416, 658, 776, 805
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
BmiI GGNNCC 3 cut(s) 230, 418, 603
BmrFI CCNGG 6 cut(s) 117, 233, 374, 520, 1054, 1084
BmsI GCATC 6 cut(s) 144, 491, 535, 679, 870, 988
BpiI GAAGAC 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 1228
Bpu1102I GCTNAGC 1 cut(s) 1269
BpuEI CTTGAG 1 cut(s) 1136
BpuMI CCSGG 2 cut(s) 233, 1054
BsaAI YACGTR 1 cut(s) 617
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 2 cut(s) 47, 78
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 115, 387, 518
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 41, 42, 203, 720, 976, 977, 1181
Bse1I ACTGG 3 cut(s) 230, 429, 714
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 4 cut(s) 117, 374, 520, 1084
BseDI CCNNGG 3 cut(s) 115, 387, 518
BseGI GGATG 4 cut(s) 418, 514, 958, 1283
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 8 cut(s) 40, 41, 42, 203, 720, 976, 977, 1181
BseMII CTCAG 3 cut(s) 114, 1101, 1260
BseNI ACTGG 3 cut(s) 230, 429, 714
BseRI GAGGAG 1 cut(s) 621
BseXI GCAGC 4 cut(s) 697, 749, 1059, 1253
BseYI CCCAGC 1 cut(s) 29
Bsh1236I CGCG 2 cut(s) 90, 150
BshFI GGCC 5 cut(s) 7, 159, 196, 981, 995
BsiSI CCGG 4 cut(s) 50, 75, 233, 1053
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 8 cut(s) 40, 41, 42, 203, 720, 976, 977, 1181
BsmAI GTCTC 2 cut(s) 76, 318
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 5 cut(s) 7, 159, 196, 981, 995
Bso31I GGTCTC 1 cut(s) 318
Bsp143I GATC 1 cut(s) 1106
Bsp1720I GCTNAGC 1 cut(s) 1269
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 7 cut(s) 26, 66, 336, 1071, 1220, 1235, 1273
BspANI GGCC 5 cut(s) 7, 159, 196, 981, 995
BspCNI CTCAG 3 cut(s) 115, 1102, 1261
BspFNI CGCG 2 cut(s) 90, 150
BspHI TCATGA 1 cut(s) 264
BspLI GGNNCC 3 cut(s) 230, 418, 603
BspMAI CTGCAG 1 cut(s) 1018
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 1 cut(s) 1114
BspQI GCTCTTC 1 cut(s) 954
BspTNI GGTCTC 1 cut(s) 318
BsrBI CCGCTC 2 cut(s) 1222, 1273
BsrI ACTGG 3 cut(s) 230, 429, 714
BssECI CCNNGG 3 cut(s) 115, 387, 518
BssMI GATC 1 cut(s) 1106
BssNI GRCGYC 2 cut(s) 47, 78
BssSI CACGAG 2 cut(s) 633, 758
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 2 cut(s) 633, 758
Bst2UI CCWGG 4 cut(s) 117, 374, 520, 1084
Bst6I CTCTTC 1 cut(s) 954
BstACI GRCGYC 2 cut(s) 47, 78
BstBAI YACGTR 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 1290
BstDEI CTNAG 5 cut(s) 123, 358, 836, 1110, 1269
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 4 cut(s) 418, 514, 958, 1283
BstFNI CGCG 2 cut(s) 90, 150
BstHHI GCGC 2 cut(s) 92, 152
BstKTI GATC 1 cut(s) 1109
BstMAI GTCTC 2 cut(s) 76, 318
BstMBI GATC 1 cut(s) 1106
BstMWI GCNNNNNNNGC 5 cut(s) 147, 156, 226, 858, 1241
BstNI CCWGG 4 cut(s) 117, 374, 520, 1084
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 6 cut(s) 115, 231, 372, 518, 1052, 1082
BstSFI CTRYAG 2 cut(s) 321, 1014
BstUI CGCG 2 cut(s) 90, 150
BstV1I GCAGC 4 cut(s) 697, 749, 1059, 1253
BstV2I GAAGAC 1 cut(s) 8
BstX2I RGATCY 1 cut(s) 1106
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 1 cut(s) 1106
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 5 cut(s) 7, 159, 196, 981, 995
BtgI CCRYGG 1 cut(s) 387
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 4 cut(s) 418, 514, 958, 1283
BtsI GCAGTG 1 cut(s) 910
BtsIMutI CAGTG 2 cut(s) 223, 910
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 1 cut(s) 1290
CaiI CAGNNNCTG 1 cut(s) 1022
CciI TCATGA 1 cut(s) 264
CfoI GCGC 2 cut(s) 92, 152
Cfr13I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
CseI GACGC 2 cut(s) 55, 67
CviAII CATG 5 cut(s) 265, 388, 653, 1030, 1256
DdeI CTNAG 5 cut(s) 123, 358, 836, 1110, 1269
DpnI GATC 1 cut(s) 1108
DpnII GATC 1 cut(s) 1106
Eam1104I CTCTTC 1 cut(s) 954
EarI CTCTTC 1 cut(s) 954
EciI GGCGGA 1 cut(s) 1060
Eco130I CCWWGG 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 318
Eco47I GGWCC 4 cut(s) 416, 658, 776, 805
Eco57I CTGAAG 1 cut(s) 606
EcoRII CCWGG 4 cut(s) 115, 372, 518, 1082
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 268, 391, 656, 1033, 1259
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 58
FatI CATG 5 cut(s) 264, 387, 652, 1029, 1255
FauI CCCGC 1 cut(s) 1266
FauNDI CATATG 1 cut(s) 975
Fnu4HI GCNGC 5 cut(s) 26, 711, 738, 1048, 1242
FokI GGATG 4 cut(s) 425, 501, 965, 1290
Fsp4HI GCNGC 5 cut(s) 26, 711, 738, 1048, 1242
FspBI CTAG 2 cut(s) 479, 879
GlaI GCGC 2 cut(s) 91, 151
GluI GCNGC 5 cut(s) 26, 711, 738, 1048, 1242
GsaI CCCAGC 1 cut(s) 33
GsuI CTGGAG 1 cut(s) 1228
HaeIII GGCC 5 cut(s) 7, 159, 196, 981, 995
HapII CCGG 4 cut(s) 50, 75, 233, 1053
HgaI GACGC 2 cut(s) 55, 67
HhaI GCGC 2 cut(s) 92, 152
Hin1I GRCGYC 2 cut(s) 47, 78
Hin1II CATG 5 cut(s) 268, 391, 656, 1033, 1259
Hin6I GCGC 2 cut(s) 90, 150
HinP1I GCGC 2 cut(s) 90, 150
HindIII AAGCTT 1 cut(s) 1127
HinfI GANTC 3 cut(s) 127, 580, 1019
HpaII CCGG 4 cut(s) 50, 75, 233, 1053
HphI GGTGA 2 cut(s) 775, 968
Hpy166II GTNNAC 5 cut(s) 244, 638, 766, 805, 943
Hpy188I TCNGA 8 cut(s) 124, 132, 399, 487, 507, 866, 1111, 1201
Hpy188III TCNNGA 5 cut(s) 20, 265, 584, 635, 644
Hpy8I GTNNAC 5 cut(s) 244, 638, 766, 805, 943
Hpy99I CGWCG 2 cut(s) 83, 90
HpyAV CCTTC 2 cut(s) 56, 710
HpyCH4IV ACGT 4 cut(s) 13, 174, 492, 616
HpyCH4V TGCA 9 cut(s) 461, 526, 679, 737, 812, 903, 1016, 1047, 1255
HpyF10VI GCNNNNNNNGC 5 cut(s) 147, 156, 226, 858, 1241
HpyF3I CTNAG 5 cut(s) 123, 358, 836, 1110, 1269
HpySE526I ACGT 4 cut(s) 13, 174, 492, 616
Hsp92I GRCGYC 2 cut(s) 47, 78
Hsp92II CATG 5 cut(s) 268, 391, 656, 1033, 1259
HspAI GCGC 2 cut(s) 90, 150
Kzo9I GATC 1 cut(s) 1106
LguI GCTCTTC 1 cut(s) 954
LmnI GCTCC 4 cut(s) 234, 671, 919, 1156
Lsp1109I GCAGC 4 cut(s) 697, 749, 1059, 1253
LweI GCATC 6 cut(s) 144, 491, 535, 679, 870, 988
MaeI CTAG 2 cut(s) 479, 879
MaeII ACGT 4 cut(s) 13, 174, 492, 616
MaeIII GTNAC 4 cut(s) 187, 382, 704, 1009
MalI GATC 1 cut(s) 1108
MbiI CCGCTC 2 cut(s) 1222, 1273
MboI GATC 1 cut(s) 1106
MboII GAAGA 2 cut(s) 8, 971
MfeI CAATTG 2 cut(s) 787, 818
MflI RGATCY 1 cut(s) 1106
MluCI AATT 9 cut(s) 38, 99, 251, 608, 684, 694, 731, 787, 818
MlyI GAGTC 1 cut(s) 574
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 4 cut(s) 50, 75, 233, 1053
MspR9I CCNGG 6 cut(s) 117, 233, 374, 520, 1054, 1084
MunI CAATTG 2 cut(s) 787, 818
MvaI CCWGG 4 cut(s) 117, 374, 520, 1084
MvnI CGCG 2 cut(s) 90, 150
MwoI GCNNNNNNNGC 5 cut(s) 147, 156, 226, 858, 1241
NciI CCSGG 2 cut(s) 233, 1054
NcoI CCATGG 1 cut(s) 387
NdeI CATATG 1 cut(s) 975
NdeII GATC 1 cut(s) 1106
NlaIII CATG 5 cut(s) 268, 391, 656, 1033, 1259
NlaIV GGNNCC 3 cut(s) 230, 418, 603
NmeAIII GCCGAG 1 cut(s) 135
NspI RCATGY 1 cut(s) 656
PagI TCATGA 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 954
PfeI GAWTC 2 cut(s) 127, 1019
PflMI CCANNNNNTGG 1 cut(s) 720
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 5 cut(s) 27, 712, 739, 1049, 1243
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
Ppu21I YACGTR 1 cut(s) 617
PsiI TTATAA 1 cut(s) 911
Psp6I CCWGG 4 cut(s) 115, 372, 518, 1082
PspFI CCCAGC 1 cut(s) 29
PspGI CCWGG 4 cut(s) 115, 372, 518, 1082
PspN4I GGNNCC 3 cut(s) 230, 418, 603
PspPI GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
PstI CTGCAG 1 cut(s) 1018
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 1 cut(s) 1106
RseI CAYNNNNRTG 1 cut(s) 425
SapI GCTCTTC 1 cut(s) 954
SatI GCNGC 5 cut(s) 26, 711, 738, 1048, 1242
Sau3AI GATC 1 cut(s) 1106
Sau96I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 6 cut(s) 117, 233, 374, 520, 1054, 1084
SfaNI GCATC 6 cut(s) 144, 491, 535, 679, 870, 988
SfcI CTRYAG 2 cut(s) 321, 1014
SinI GGWCC 4 cut(s) 416, 658, 776, 805
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 1151
SmoI CTYRAG 1 cut(s) 1151
Sse9I AATT 9 cut(s) 38, 99, 251, 608, 684, 694, 731, 787, 818
SsiI CCGC 7 cut(s) 26, 66, 336, 1071, 1220, 1235, 1273
SspMI CTAG 2 cut(s) 479, 879
StyD4I CCNGG 6 cut(s) 115, 231, 372, 518, 1052, 1082
StyI CCWWGG 1 cut(s) 387
TaiI ACGT 4 cut(s) 16, 177, 495, 619
TaqI TCGA 3 cut(s) 184, 1171, 1249
TasI AATT 9 cut(s) 38, 99, 251, 608, 684, 694, 731, 787, 818
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 2 cut(s) 127, 1019
TscAI CASTG 2 cut(s) 230, 910
TseI GCWGC 4 cut(s) 710, 737, 1047, 1241
TspDTI ATGAA 4 cut(s) 420, 744, 1242, 1272
TspGWI ACGGA 2 cut(s) 425, 445
TspRI CASTG 2 cut(s) 230, 910
Van91I CCANNNNNTGG 1 cut(s) 720
VpaK11BI GGWCC 4 cut(s) 416, 658, 776, 805
XapI RAATTY 1 cut(s) 731
XceI RCATGY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XspI CTAG 2 cut(s) 479, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.