RchiOBHm_Chr5g0046451

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005245
Physical Location & Seq
Forward (+)
42503935 .. 42508874
4940 bp
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UTR
Exon/CDS
Intron
PRQ32444

Sequence Viewer

Length: 828 bp
ATGGCTCGTCGGACTTATGAAAAGGATGGACCCATACTGGCGGTAGCTTGTGGCAGGGATACTATTTCCATTGTAAGTTCTATTAGACGACTAGCATCTGAAAACATCTTTGTTGTTCAGATACAACATCCCAGGTTGCATCTCAATAGATTTGACTTGGATTTTACCCCGAAACACGATTACTATCCTTTGACTCCTGAATCACAGAAGCAGGTTCCTCAATTTCTACGTGGGTGGATAACTCCTCGTGAACCTCCTGACAGGCATGTGGTCCTCACTATTAGAGCTTTGCATCAAATTGATGCTACTGCCCTTCGTAATGCTGCCAGTGCATGGCGTGATGAATTTGCAGCTCTACCAAAGCCCTTACTTGTGGTGAACATTGGTGGTCCTACAAGCAATTGTCGTTATGGTGTGGACCTTGCGAAGCAATTGACAACCTATTTGCTTAGTGTTCTTGTATCCAATTTTGTAACAAAGGAGCTCGGTGGTAATCCAAAAGTTTACACTTGGGATGGTCAAGAGCCAAACCCGCACATGGGCCATCTAGCTTGGGTCGATGCTTTTGTTGTCACCGCAGATTCTGTTAGCATGATAAGTGAGGTTTGCAGCACCGGGAAGCCTGTCTATCTAATGGGAGCTGAGCATTGTAAATGGAAGCTTTGTGATTTCCATAAATCACTCAAGGAGCGAGGAATGGTTCGGCCATTTACAGGCTTGGAGAATATTTCAGAGAGCTGGAGTTATCCACCGCTCAATGAAACCGCAGAAGCAGCCAGTCGAGTGCATGAAGCCCTTGCTGAGCGGGGATGGAGATTGTGGGCATAG

Protein Analysis

275

Amino Acids

31.03

Weight (kDa)

8.25

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 1 - 266 5.9e-81 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccB7I CCANNNNNTGG 1 cut(s) 333
AccBSI CCGCTC 2 cut(s) 754, 805
AciI CCGC 6 cut(s) 41, 533, 576, 752, 765, 805
AcoI YGGCCR 1 cut(s) 704
AcsI RAATTY 1 cut(s) 344
AfiI CCNNNNNNNGG 4 cut(s) 333, 538, 539, 713
AjnI CCWGG 1 cut(s) 131
AluBI AGCT 8 cut(s) 47, 287, 353, 484, 551, 641, 661, 738
AluI AGCT 8 cut(s) 47, 287, 353, 484, 551, 641, 661, 738
Alw21I GWGCWC 1 cut(s) 486
AlwNI CAGNNNCTG 1 cut(s) 584
AoxI GGCC 2 cut(s) 541, 704
ApeKI GCWGC 4 cut(s) 323, 350, 609, 773
ApoI RAATTY 1 cut(s) 344
ArsI GACNNNNNNTTYG 2 cut(s) 427, 459
AspS9I GGNCC 5 cut(s) 29, 271, 389, 418, 541
AsuC2I CCSGG 1 cut(s) 616
AsuHPI GGTGA 2 cut(s) 388, 565
AvaII GGWCC 4 cut(s) 29, 271, 389, 418
BanII GRGCYC 1 cut(s) 486
BauI CACGAG 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 4 cut(s) 310, 362, 621, 785
BccI CCATC 4 cut(s) 20, 509, 552, 804
BciT130I CCWGG 1 cut(s) 133
BciVI GTATCC 2 cut(s) 52, 472
BcnI CCSGG 1 cut(s) 616
BfaI CTAG 2 cut(s) 92, 548
BfuAI ACCTGC 1 cut(s) 202
BfuI GTATCC 2 cut(s) 52, 472
BisI GCNGC 4 cut(s) 324, 351, 610, 774
BlpI GCTNAGC 2 cut(s) 642, 801
BlsI GCNGC 4 cut(s) 325, 352, 611, 775
Bme1390I CCNGG 2 cut(s) 133, 616
Bme18I GGWCC 4 cut(s) 29, 271, 389, 418
BmgT120I GGNCC 5 cut(s) 29, 271, 389, 418, 541
BmiI GGNNCC 2 cut(s) 31, 216
BmrFI CCNGG 2 cut(s) 133, 616
BmsI GCATC 5 cut(s) 104, 148, 292, 301, 550
BpmI CTGGAG 1 cut(s) 760
Bpu1102I GCTNAGC 2 cut(s) 642, 801
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 616
BsaAI YACGTR 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 131
Bsc4I CCNNNNNNNGG 4 cut(s) 333, 538, 539, 713
Bse1I ACTGG 3 cut(s) 42, 327, 777
Bse8I GATNNNNATC 1 cut(s) 183
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 131
BseGI GGATG 4 cut(s) 31, 127, 520, 815
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 4 cut(s) 333, 538, 539, 713
BseMII CTCAG 2 cut(s) 633, 792
BseNI ACTGG 3 cut(s) 42, 327, 777
BseRI GAGGAG 1 cut(s) 234
BseXI GCAGC 4 cut(s) 310, 362, 621, 785
BshFI GGCC 2 cut(s) 543, 706
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 1 cut(s) 615
BslI CCNNNNNNNGG 4 cut(s) 333, 538, 539, 713
BsnI GGCC 2 cut(s) 543, 706
Bsp1286I GDGCHC 1 cut(s) 486
Bsp1720I GCTNAGC 2 cut(s) 642, 801
BspACI CCGC 6 cut(s) 41, 533, 576, 752, 765, 805
BspANI GGCC 2 cut(s) 543, 706
BspCNI CTCAG 2 cut(s) 634, 793
BspLI GGNNCC 2 cut(s) 31, 216
BspMI ACCTGC 1 cut(s) 202
BsrBI CCGCTC 2 cut(s) 754, 805
BsrI ACTGG 3 cut(s) 42, 327, 777
BssECI CCNNGG 1 cut(s) 131
BssSI CACGAG 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 133
BstBAI YACGTR 1 cut(s) 230
BstDEI CTNAG 3 cut(s) 449, 642, 801
BstF5I GGATG 4 cut(s) 31, 127, 520, 815
BstMWI GCNNNNNNNGC 3 cut(s) 329, 532, 773
BstNI CCWGG 1 cut(s) 133
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 131, 614
BstV1I GCAGC 4 cut(s) 310, 362, 621, 785
BsuI GTATCC 2 cut(s) 52, 472
BsuRI GGCC 2 cut(s) 543, 706
BtsCI GGATG 4 cut(s) 31, 127, 520, 815
BtsIMutI CAGTG 1 cut(s) 334
BveI ACCTGC 1 cut(s) 202
CaiI CAGNNNCTG 1 cut(s) 584
Cfr13I GGNCC 5 cut(s) 29, 271, 389, 418, 541
CviAII CATG 5 cut(s) 266, 333, 538, 592, 788
DdeI CTNAG 3 cut(s) 449, 642, 801
EaeI YGGCCR 1 cut(s) 704
Ecl136II GAGCTC 1 cut(s) 484
Eco24I GRGCYC 1 cut(s) 486
Eco47I GGWCC 4 cut(s) 29, 271, 389, 418
Eco53kI GAGCTC 1 cut(s) 484
EcoICRI GAGCTC 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 131
EcoT38I GRGCYC 1 cut(s) 486
FaeI CATG 5 cut(s) 269, 336, 541, 595, 791
FatI CATG 5 cut(s) 265, 332, 537, 591, 787
FauI CCCGC 2 cut(s) 540, 798
Fnu4HI GCNGC 4 cut(s) 324, 351, 610, 774
FokI GGATG 4 cut(s) 38, 114, 527, 822
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 4 cut(s) 324, 351, 610, 774
FspBI CTAG 2 cut(s) 92, 548
GluI GCNGC 4 cut(s) 324, 351, 610, 774
GsuI CTGGAG 1 cut(s) 760
HaeIII GGCC 2 cut(s) 543, 706
HapII CCGG 1 cut(s) 615
Hin1II CATG 5 cut(s) 269, 336, 541, 595, 791
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 3 cut(s) 193, 200, 581
HpaII CCGG 1 cut(s) 615
HphI GGTGA 2 cut(s) 388, 565
Hpy166II GTNNAC 4 cut(s) 251, 379, 418, 505
Hpy188I TCNGA 4 cut(s) 12, 100, 120, 733
Hpy188III TCNNGA 4 cut(s) 197, 248, 257, 521
Hpy8I GTNNAC 4 cut(s) 251, 379, 418, 505
Hpy99I CGWCG 1 cut(s) 12
HpyAV CCTTC 1 cut(s) 323
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 6 cut(s) 139, 292, 332, 350, 609, 787
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 532, 773
HpyF3I CTNAG 3 cut(s) 449, 642, 801
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 5 cut(s) 269, 336, 541, 595, 791
LmnI GCTCC 3 cut(s) 481, 638, 688
Lsp1109I GCAGC 4 cut(s) 310, 362, 621, 785
LweI GCATC 5 cut(s) 104, 148, 292, 301, 550
MaeI CTAG 2 cut(s) 92, 548
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 2 cut(s) 472, 571
MbiI CCGCTC 2 cut(s) 754, 805
MfeI CAATTG 2 cut(s) 400, 431
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 6 cut(s) 221, 297, 344, 400, 431, 466
MlyI GAGTC 1 cut(s) 187
MnlI CCTC 6 cut(s) 228, 255, 264, 284, 595, 686
MspI CCGG 1 cut(s) 615
MspR9I CCNGG 2 cut(s) 133, 616
MunI CAATTG 2 cut(s) 400, 431
MvaI CCWGG 1 cut(s) 133
MwoI GCNNNNNNNGC 3 cut(s) 329, 532, 773
NciI CCSGG 1 cut(s) 616
NlaIII CATG 5 cut(s) 269, 336, 541, 595, 791
NlaIV GGNNCC 2 cut(s) 31, 216
NmuCI GTSAC 1 cut(s) 571
NspI RCATGY 1 cut(s) 269
PfeI GAWTC 2 cut(s) 200, 581
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 4 cut(s) 325, 352, 611, 775
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 31, 216
PspPI GGNCC 5 cut(s) 29, 271, 389, 418, 541
PstNI CAGNNNCTG 1 cut(s) 584
SacI GAGCTC 1 cut(s) 486
SatI GCNGC 4 cut(s) 324, 351, 610, 774
Sau96I GGNCC 5 cut(s) 29, 271, 389, 418, 541
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 2 cut(s) 133, 616
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 5 cut(s) 104, 148, 292, 301, 550
SinI GGWCC 4 cut(s) 29, 271, 389, 418
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 6 cut(s) 221, 297, 344, 400, 431, 466
SsiI CCGC 6 cut(s) 41, 533, 576, 752, 765, 805
SspI AATATT 1 cut(s) 727
SspMI CTAG 2 cut(s) 92, 548
SstI GAGCTC 1 cut(s) 486
StyD4I CCNGG 2 cut(s) 131, 614
TaiI ACGT 1 cut(s) 232
TaqI TCGA 2 cut(s) 558, 781
TasI AATT 6 cut(s) 221, 297, 344, 400, 431, 466
TfiI GAWTC 2 cut(s) 200, 581
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 571
TseI GCWGC 4 cut(s) 323, 350, 609, 773
Tsp45I GTSAC 1 cut(s) 571
TspDTI ATGAA 4 cut(s) 33, 357, 774, 804
TspRI CASTG 1 cut(s) 334
Van91I CCANNNNNTGG 1 cut(s) 333
VpaK11BI GGWCC 4 cut(s) 29, 271, 389, 418
XapI RAATTY 1 cut(s) 344
XceI RCATGY 1 cut(s) 269
XspI CTAG 2 cut(s) 92, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.