RchiOBHm_Chr5g0046541

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005245
Physical Location & Seq
Reverse (-)
42595177 .. 42602780
7604 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32453

Sequence Viewer

Length: 1266 bp
ATGAGGCCGATACGTCTTCCTGAGCCGCCCAGCCCCAAAATGGGGACGCCGGAAATCTTCGAAGGCGGCGTATACGGCGTCGTACGCCGCGCCGTCGTGATTGGCAACGGCTTCCCCGGTTCCGAGAATCAGAGTATCGGCCTGGTTCGCGCTCTCGGCCTCGCCGACAAGCACGTCGTGTATCGAGTTACAAGGCCAAGAGGAGGTCTAAATGAGTGGCTTCACTGGCTCCCGGTTTCTGTTCACAAGAAGTTGGATTGTGTCATAAGGAAGATGTGTATTTTGTTAATATATCGGGGGCAGAAAGTTGGGTCTCTACCAACAGAAAATGGTGGCAGTGCTGGGTTGAAATGTGTCTTAGAAGCTGATGTCCAGGAGATTGTAACCATGGCTCGTCAGACTTATGAAAAGGATGGACCCATACTGGTGGTAGCTTGTGGCAGGGATACTATTTCCATTGCAAGTTCTCTTAGACGACTAGCATCTGAAAACGTCTTTGTTGTACAGATACAACATCCTAGGTTGCATCTCAATAGATTTGACTTGGTTGTTACCCCGAAACACGATTACTATCCTTTGACCCCTGAAGCACAGAAGCAGGTTCCTCAATTTCTACGTGGGTGGATAACTCCTCGTGAACCTCCTGACAGGCATGTGGTCCTCACTATTGGAGCTTTGCATCAAATTGATGCTACTGCCCTTCGTAATGCTGCCAGTGCATGGCGTGATGAATTTGCAGCTCTACCAAAGCCCTTACTTGTGGCGAACATTGGTGGTCCTACAAGCAATTGTCGTTATGGTGTGGACCTTGCGAAGCAATTGGCAACCTATTTGCTTAGTGTTCTTGTAAGCTGTGGAAGCATCAGAATATGTTTCTCTAGTAGAACACCTGAAAAGGTATCCAATGTTATAACAAAGGAGCTCGGAGGTAATCCAAAAGTTTACATTTGGGATGGTCAAGAGCCAAACCCGTACATGGGCCATCTGGCCTGGGCTGATGCTTTTGTTGTCACCGCAGATTCCGTTAGCATGATAAGTGAGGTTTGCAGCACCGGGAAACCTGTGTATCTAATGGGAGCTGAGCGTTGTAAATGGAAGCTTTGTGATTTCCATAAATCACTCAAGGAGCGAGGAATGGTTCGGCCATTTACAGGCTTGGAGAATATTTCAGAGAGCTGGAGTTATCCACCGCTCAATGAAACTGCAGAAGCAGCGAGTCGAGTGCATCAAGCCCTTGCTGAACGGGGATGGAGATTGCGGGCATAG

Protein Analysis

421

Amino Acids

46.64

Weight (kDa)

9.23

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 39 - 412 1.2e-109 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 911
AasI GACNNNNNNGTC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 589
AccB7I CCANNNNNTGG 1 cut(s) 720
AccBSI CCGCTC 1 cut(s) 1192
AccI GTMKAC 1 cut(s) 72
AccII CGCG 2 cut(s) 90, 150
AciI CCGC 6 cut(s) 26, 66, 88, 1014, 1190, 1258
AcoI YGGCCR 1 cut(s) 1142
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 1 cut(s) 606
AcyI GRCGYC 2 cut(s) 47, 78
AdeI CACNNNGTG 1 cut(s) 178
AfaI GTAC 3 cut(s) 84, 504, 974
AfiI CCNNNNNNNGG 8 cut(s) 40, 41, 42, 203, 720, 976, 977, 1151
AgsI TTSAA 1 cut(s) 349
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 3 cut(s) 141, 372, 989
AluBI AGCT 9 cut(s) 365, 434, 674, 740, 852, 922, 1079, 1099, 1176
AluI AGCT 9 cut(s) 365, 434, 674, 740, 852, 922, 1079, 1099, 1176
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 1 cut(s) 318
AoxI GGCC 7 cut(s) 5, 139, 157, 194, 979, 987, 1142
ApeKI GCWGC 4 cut(s) 710, 737, 1047, 1211
ApoI RAATTY 1 cut(s) 731
AspA2I CCTAGG 1 cut(s) 518
AspLEI GCGC 2 cut(s) 92, 152
AspS9I GGNCC 5 cut(s) 416, 658, 776, 805, 979
AsuC2I CCSGG 3 cut(s) 117, 233, 1054
AsuHPI GGTGA 1 cut(s) 1003
AsuII TTCGAA 1 cut(s) 60
AvaII GGWCC 4 cut(s) 416, 658, 776, 805
AvrII CCTAGG 1 cut(s) 518
BaeI ACNNNNGTAYC 2 cut(s) 1049, 1082
BanII GRGCYC 1 cut(s) 924
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 4 cut(s) 697, 749, 1059, 1223
BccI CCATC 4 cut(s) 407, 947, 990, 1242
BceAI ACGGC 3 cut(s) 77, 91, 124
BcgI CGANNNNNNTGC 2 cut(s) 1204, 1238
BciT130I CCWGG 3 cut(s) 143, 374, 991
BciVI GTATCC 2 cut(s) 439, 910
BcnI CCSGG 3 cut(s) 117, 233, 1054
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 3 cut(s) 479, 519, 879
BfmI CTRYAG 1 cut(s) 1203
BfuAI ACCTGC 1 cut(s) 589
BfuI GTATCC 2 cut(s) 439, 910
BisI GCNGC 7 cut(s) 26, 67, 88, 711, 738, 1048, 1212
BlnI CCTAGG 1 cut(s) 518
BlpI GCTNAGC 1 cut(s) 1080
BlsI GCNGC 7 cut(s) 27, 68, 89, 712, 739, 1049, 1213
Bme1390I CCNGG 6 cut(s) 117, 143, 233, 374, 991, 1054
Bme18I GGWCC 4 cut(s) 416, 658, 776, 805
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 5 cut(s) 416, 658, 776, 805, 979
BmiI GGNNCC 4 cut(s) 121, 230, 418, 603
BmrFI CCNGG 6 cut(s) 117, 143, 233, 374, 991, 1054
BmsI GCATC 7 cut(s) 491, 535, 679, 688, 870, 988, 1234
BpiI GAAGAC 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 1198
Bpu10I CCTNAGC 1 cut(s) 21
Bpu1102I GCTNAGC 1 cut(s) 1080
Bpu14I TTCGAA 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 1106
BpuMI CCSGG 3 cut(s) 117, 233, 1054
BsaAI YACGTR 1 cut(s) 617
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 2 cut(s) 47, 78
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 115, 387, 518, 990
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 41, 42, 203, 720, 976, 977, 1151
Bse1I ACTGG 3 cut(s) 230, 429, 714
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 3 cut(s) 143, 374, 991
BseDI CCNNGG 4 cut(s) 115, 387, 518, 990
BseGI GGATG 4 cut(s) 418, 514, 958, 1253
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 8 cut(s) 40, 41, 42, 203, 720, 976, 977, 1151
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 2 cut(s) 12, 1071
BseNI ACTGG 3 cut(s) 230, 429, 714
BseRI GAGGAG 2 cut(s) 216, 621
BseXI GCAGC 4 cut(s) 697, 749, 1059, 1223
BseYI CCCAGC 2 cut(s) 29, 341
Bsh1236I CGCG 2 cut(s) 90, 150
BshFI GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 1144
BsiHKAI GWGCWC 1 cut(s) 924
BsiSI CCGG 4 cut(s) 50, 117, 233, 1053
BsiWI CGTACG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 8 cut(s) 40, 41, 42, 203, 720, 976, 977, 1151
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 1144
Bso31I GGTCTC 1 cut(s) 318
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 924
Bsp1407I TGTACA 1 cut(s) 502
Bsp1720I GCTNAGC 1 cut(s) 1080
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 6 cut(s) 26, 66, 88, 1014, 1190, 1258
BspANI GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 1144
BspCNI CTCAG 2 cut(s) 13, 1072
BspFNI CGCG 2 cut(s) 90, 150
BspLI GGNNCC 4 cut(s) 121, 230, 418, 603
BspMAI CTGCAG 1 cut(s) 1207
BspMI ACCTGC 1 cut(s) 589
BspT104I TTCGAA 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 318
BsrBI CCGCTC 1 cut(s) 1192
BsrDI GCAATG 1 cut(s) 456
BsrGI TGTACA 1 cut(s) 502
BsrI ACTGG 3 cut(s) 230, 429, 714
BssECI CCNNGG 4 cut(s) 115, 387, 518, 990
BssNAI GTATAC 1 cut(s) 73
BssNI GRCGYC 2 cut(s) 47, 78
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 2 cut(s) 387, 518
Bst1107I GTATAC 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 3 cut(s) 143, 374, 991
BstACI GRCGYC 2 cut(s) 47, 78
BstAUI TGTACA 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 1260
BstDEI CTNAG 5 cut(s) 21, 358, 470, 836, 1080
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 4 cut(s) 418, 514, 958, 1253
BstFNI CGCG 2 cut(s) 90, 150
BstHHI GCGC 2 cut(s) 92, 152
BstMAI GTCTC 1 cut(s) 318
BstMWI GCNNNNNNNGC 8 cut(s) 75, 84, 147, 156, 226, 716, 858, 1211
BstNI CCWGG 3 cut(s) 143, 374, 991
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 6 cut(s) 115, 141, 231, 372, 989, 1052
BstSFI CTRYAG 1 cut(s) 1203
BstUI CGCG 2 cut(s) 90, 150
BstV1I GCAGC 4 cut(s) 697, 749, 1059, 1223
BstV2I GAAGAC 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 427
BstZ17I GTATAC 1 cut(s) 73
BsuI GTATCC 2 cut(s) 439, 910
BsuRI GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 1144
BtgI CCRYGG 1 cut(s) 387
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 4 cut(s) 418, 514, 958, 1253
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 3 cut(s) 223, 343, 721
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 1 cut(s) 1260
CfoI GCGC 2 cut(s) 92, 152
Cfr13I GGNCC 5 cut(s) 416, 658, 776, 805, 979
CseI GACGC 2 cut(s) 55, 67
Csp6I GTAC 3 cut(s) 83, 503, 973
CviAII CATG 5 cut(s) 388, 653, 720, 976, 1030
CviQI GTAC 3 cut(s) 83, 503, 973
DdeI CTNAG 5 cut(s) 21, 358, 470, 836, 1080
DraIII CACNNNGTG 1 cut(s) 178
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 1142
Ecl136II GAGCTC 1 cut(s) 922
Eco130I CCWWGG 2 cut(s) 387, 518
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 1 cut(s) 318
Eco47I GGWCC 4 cut(s) 416, 658, 776, 805
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 606
EcoICRI GAGCTC 1 cut(s) 922
EcoRII CCWGG 3 cut(s) 141, 372, 989
EcoT14I CCWWGG 2 cut(s) 387, 518
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 2 cut(s) 387, 518
FaeI CATG 5 cut(s) 391, 656, 723, 979, 1033
FaqI GGGAC 1 cut(s) 58
FatI CATG 5 cut(s) 387, 652, 719, 975, 1029
FauI CCCGC 1 cut(s) 1251
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 7 cut(s) 26, 67, 88, 711, 738, 1048, 1212
FokI GGATG 4 cut(s) 425, 501, 965, 1260
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 7 cut(s) 26, 67, 88, 711, 738, 1048, 1212
FspBI CTAG 3 cut(s) 479, 519, 879
GlaI GCGC 2 cut(s) 91, 151
GluI GCNGC 7 cut(s) 26, 67, 88, 711, 738, 1048, 1212
GsaI CCCAGC 2 cut(s) 33, 345
GsuI CTGGAG 1 cut(s) 1198
HaeIII GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 1144
HapII CCGG 4 cut(s) 50, 117, 233, 1053
HgaI GACGC 2 cut(s) 55, 67
HhaI GCGC 2 cut(s) 92, 152
Hin1I GRCGYC 2 cut(s) 47, 78
Hin1II CATG 5 cut(s) 391, 656, 723, 979, 1033
Hin6I GCGC 2 cut(s) 90, 150
HinP1I GCGC 2 cut(s) 90, 150
HindIII AAGCTT 1 cut(s) 1097
HinfI GANTC 3 cut(s) 127, 1019, 1216
HpaII CCGG 4 cut(s) 50, 117, 233, 1053
HphI GGTGA 1 cut(s) 1003
Hpy166II GTNNAC 5 cut(s) 73, 244, 638, 805, 943
Hpy188I TCNGA 7 cut(s) 124, 132, 399, 487, 866, 926, 1171
Hpy188III TCNNGA 5 cut(s) 20, 97, 635, 644, 959
Hpy8I GTNNAC 5 cut(s) 73, 244, 638, 805, 943
Hpy99I CGWCG 3 cut(s) 83, 98, 179
HpyAV CCTTC 2 cut(s) 56, 710
HpyCH4IV ACGT 4 cut(s) 13, 174, 492, 616
HpyCH4V TGCA 8 cut(s) 461, 526, 679, 719, 737, 1047, 1205, 1225
HpyF10VI GCNNNNNNNGC 8 cut(s) 75, 84, 147, 156, 226, 716, 858, 1211
HpyF3I CTNAG 5 cut(s) 21, 358, 470, 836, 1080
HpySE526I ACGT 4 cut(s) 13, 174, 492, 616
Hsp92I GRCGYC 2 cut(s) 47, 78
Hsp92II CATG 5 cut(s) 391, 656, 723, 979, 1033
HspAI GCGC 2 cut(s) 90, 150
LmnI GCTCC 5 cut(s) 234, 671, 919, 1076, 1126
Lsp1109I GCAGC 4 cut(s) 697, 749, 1059, 1223
LweI GCATC 7 cut(s) 491, 535, 679, 688, 870, 988, 1234
MaeI CTAG 3 cut(s) 479, 519, 879
MaeII ACGT 4 cut(s) 13, 174, 492, 616
MaeIII GTNAC 4 cut(s) 187, 382, 550, 1009
MbiI CCGCTC 1 cut(s) 1192
MboII GAAGA 3 cut(s) 8, 49, 283
MfeI CAATTG 2 cut(s) 787, 818
MhlI GDGCHC 1 cut(s) 924
MluCI AATT 5 cut(s) 608, 684, 731, 787, 818
MlyI GAGTC 1 cut(s) 1225
MmeI TCCRAC 1 cut(s) 234
MseI TTAA 1 cut(s) 287
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 4 cut(s) 50, 117, 233, 1053
MspR9I CCNGG 6 cut(s) 117, 143, 233, 374, 991, 1054
MunI CAATTG 2 cut(s) 787, 818
MvaI CCWGG 3 cut(s) 143, 374, 991
MvnI CGCG 2 cut(s) 90, 150
MwoI GCNNNNNNNGC 8 cut(s) 75, 84, 147, 156, 226, 716, 858, 1211
NciI CCSGG 3 cut(s) 117, 233, 1054
NcoI CCATGG 1 cut(s) 387
NlaIII CATG 5 cut(s) 391, 656, 723, 979, 1033
NlaIV GGNNCC 4 cut(s) 121, 230, 418, 603
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 1009
NspI RCATGY 1 cut(s) 656
NspV TTCGAA 1 cut(s) 60
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 162
PfeI GAWTC 2 cut(s) 127, 1019
Pfl23II CGTACG 1 cut(s) 82
PflMI CCANNNNNTGG 1 cut(s) 720
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 7 cut(s) 27, 68, 89, 712, 739, 1049, 1213
PleI GAGTC 1 cut(s) 1224
PpsI GAGTC 1 cut(s) 1224
Ppu21I YACGTR 1 cut(s) 617
PsiI TTATAA 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 924
Psp6I CCWGG 3 cut(s) 141, 372, 989
PspFI CCCAGC 2 cut(s) 29, 341
PspGI CCWGG 3 cut(s) 141, 372, 989
PspLI CGTACG 1 cut(s) 82
PspN4I GGNNCC 4 cut(s) 121, 230, 418, 603
PspPI GGNCC 5 cut(s) 416, 658, 776, 805, 979
PstI CTGCAG 1 cut(s) 1207
RsaI GTAC 3 cut(s) 84, 504, 974
RsaNI GTAC 3 cut(s) 83, 503, 973
RseI CAYNNNNRTG 1 cut(s) 425
SacI GAGCTC 1 cut(s) 924
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 7 cut(s) 26, 67, 88, 711, 738, 1048, 1212
Sau96I GGNCC 5 cut(s) 416, 658, 776, 805, 979
SchI GAGTC 1 cut(s) 1225
ScrFI CCNGG 6 cut(s) 117, 143, 233, 374, 991, 1054
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 7 cut(s) 491, 535, 679, 688, 870, 988, 1234
SfcI CTRYAG 1 cut(s) 1203
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 4 cut(s) 416, 658, 776, 805
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 1121
SmoI CTYRAG 1 cut(s) 1121
Sse9I AATT 5 cut(s) 608, 684, 731, 787, 818
SsiI CCGC 6 cut(s) 26, 66, 88, 1014, 1190, 1258
SspI AATATT 1 cut(s) 1165
SspMI CTAG 3 cut(s) 479, 519, 879
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 6 cut(s) 115, 141, 231, 372, 989, 1052
StyI CCWWGG 2 cut(s) 387, 518
TaiI ACGT 4 cut(s) 16, 177, 495, 619
TaqI TCGA 3 cut(s) 60, 184, 1219
TasI AATT 5 cut(s) 608, 684, 731, 787, 818
TatI WGTACW 1 cut(s) 502
TauI GCSGC 3 cut(s) 28, 69, 90
TfiI GAWTC 2 cut(s) 127, 1019
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 3 cut(s) 230, 343, 721
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 4 cut(s) 710, 737, 1047, 1211
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 3 cut(s) 420, 744, 1212
TspGWI ACGGA 1 cut(s) 1012
TspRI CASTG 3 cut(s) 230, 343, 721
Van91I CCANNNNNTGG 1 cut(s) 720
VpaK11BI GGWCC 4 cut(s) 416, 658, 776, 805
XapI RAATTY 1 cut(s) 731
XceI RCATGY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmaJI CCTAGG 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 3 cut(s) 479, 519, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.