RchiOBHm_Chr5g0046461

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005245
Physical Location & Seq
Reverse (-)
42521681 .. 42527287
5607 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32445

Sequence Viewer

Length: 765 bp
ATGGAAATCCATTCTTTTCTGCGAGTTTCAAGGCCAAGAGGTGGTCTAAATGAGTGGCTTCACTGGCTCCCGGTTTCTGTTCACAAGAAGTTGGATTGCGTCGTAAGGAAGATGTGTATTTTCTTAATATATCGGGGCCAGAAAGTTGGGTCTCTACCAACAGAAAATGGTGGCAGTGCTGGGTTGAAATGTGTCTTAGAAGCTGATGTCCAGGAGATTGTAACCATGGCTTGTCGGACTTATGAGAAGGATGGACCCATACTGTGGGTAACTTGTGGCAGGGATACTATTTCCATTGTAAGTTCTATTAGACGACTAGCATCTGAAAACATCTTTGTTGTTCTGGTTTATACAACATCCGATAGATTTGACTTGGATTTTACCCCGAAACACGATTACTATCCTTTGACTCCTGAAGCACAGAAGCAGGTTCCTCAATTTCTACGTGGGTGGATAACTCCTCGTGAACCTCCTGACAGGCATGTCCTCACTATTGGAGCTTTGCATCAAATTGATGCTACTGCCCTTCGAAATGCTGCCAGTGCATGGCGTGCTGAATTTGCAGCTTTACCAAAGCCCTTACTTGTGGTGAACATTGGTGGTCCTACTAGCAATTGTCGTTATGGTGTGGACCTTGCGAAGCAATTGACAACCTATTTGCTTAGTGTTCTTGACCGGCCCTGTCTACATGTATCCAATTTTATAACAAAGGAGCTCGGTTGTAATCCAAAAGTTTACACTTGGGATGGTCAAGGTAACATGTGA

Protein Analysis

254

Amino Acids

28.55

Weight (kDa)

8.76

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 38 - 226 7.7e-34 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AasI GACNNNNNNGTC 1 cut(s) 482
Acc36I ACCTGC 1 cut(s) 418
AccB7I CCANNNNNTGG 3 cut(s) 41, 264, 546
AccI GTMKAC 1 cut(s) 685
AcsI RAATTY 1 cut(s) 557
AcuI CTGAAG 1 cut(s) 435
AfiI CCNNNNNNNGG 3 cut(s) 41, 264, 546
AflIII ACRYGT 2 cut(s) 688, 759
AgsI TTSAA 2 cut(s) 30, 187
AjnI CCWGG 1 cut(s) 210
AluBI AGCT 4 cut(s) 203, 500, 566, 715
AluI AGCT 4 cut(s) 203, 500, 566, 715
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 1 cut(s) 156
AoxI GGCC 3 cut(s) 32, 136, 677
ApeKI GCWGC 2 cut(s) 536, 563
ApoI RAATTY 1 cut(s) 557
ArsI GACNNNNNNTTYG 2 cut(s) 640, 672
AspS9I GGNCC 5 cut(s) 136, 254, 602, 631, 678
AsuC2I CCSGG 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 601
AsuII TTCGAA 1 cut(s) 529
AvaII GGWCC 3 cut(s) 254, 602, 631
BanII GRGCYC 1 cut(s) 717
BauI CACGAG 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 2 cut(s) 523, 575
BccI CCATC 2 cut(s) 245, 740
BciT130I CCWGG 1 cut(s) 212
BciVI GTATCC 2 cut(s) 277, 703
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 2 cut(s) 317, 609
BfuAI ACCTGC 1 cut(s) 418
BfuI GTATCC 2 cut(s) 277, 703
BisI GCNGC 2 cut(s) 537, 564
BlsI GCNGC 2 cut(s) 538, 565
Bme1390I CCNGG 2 cut(s) 71, 212
Bme18I GGWCC 3 cut(s) 254, 602, 631
BmgT120I GGNCC 5 cut(s) 136, 254, 602, 631, 678
BmiI GGNNCC 4 cut(s) 68, 137, 256, 432
BmrFI CCNGG 2 cut(s) 71, 212
BmsI GCATC 3 cut(s) 329, 505, 514
Bpu14I TTCGAA 1 cut(s) 529
BpuMI CCSGG 1 cut(s) 71
BsaAI YACGTR 1 cut(s) 446
BsaBI GATNNNNATC 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 225
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 264, 546
Bse118I RCCGGY 1 cut(s) 675
Bse1I ACTGG 2 cut(s) 68, 540
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 3 cut(s) 256, 356, 751
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 3 cut(s) 41, 264, 546
BseNI ACTGG 2 cut(s) 68, 540
BseRI GAGGAG 1 cut(s) 450
BseXI GCAGC 2 cut(s) 523, 575
BseYI CCCAGC 1 cut(s) 179
BshFI GGCC 3 cut(s) 34, 138, 679
BsiHKAI GWGCWC 1 cut(s) 717
BsiSI CCGG 2 cut(s) 71, 676
BslI CCNNNNNNNGG 3 cut(s) 41, 264, 546
BsmAI GTCTC 1 cut(s) 156
BsnI GGCC 3 cut(s) 34, 138, 679
Bso31I GGTCTC 1 cut(s) 156
Bsp119I TTCGAA 1 cut(s) 529
Bsp1286I GDGCHC 1 cut(s) 717
Bsp19I CCATGG 1 cut(s) 225
BspANI GGCC 3 cut(s) 34, 138, 679
BspLI GGNNCC 4 cut(s) 68, 137, 256, 432
BspMI ACCTGC 1 cut(s) 418
BspT104I TTCGAA 1 cut(s) 529
BspTNI GGTCTC 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 675
BsrI ACTGG 2 cut(s) 68, 540
BssAI RCCGGY 1 cut(s) 675
BssECI CCNNGG 1 cut(s) 225
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 551
BstBAI YACGTR 1 cut(s) 446
BstBI TTCGAA 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 2 cut(s) 196, 662
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 3 cut(s) 256, 356, 751
BstMAI GTCTC 1 cut(s) 156
BstMWI GCNNNNNNNGC 4 cut(s) 64, 542, 551, 560
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 3 cut(s) 485, 692, 763
BstSCI CCNGG 2 cut(s) 69, 210
BstV1I GCAGC 2 cut(s) 523, 575
BstXI CCANNNNNNTGG 1 cut(s) 146
BsuI GTATCC 2 cut(s) 277, 703
BsuRI GGCC 3 cut(s) 34, 138, 679
BtgI CCRYGG 1 cut(s) 225
BtsCI GGATG 3 cut(s) 256, 356, 751
BtsI GCAGTG 1 cut(s) 181
BtsIMutI CAGTG 3 cut(s) 61, 181, 547
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 1 cut(s) 552
Cfr10I RCCGGY 1 cut(s) 675
Cfr13I GGNCC 5 cut(s) 136, 254, 602, 631, 678
CseI GACGC 1 cut(s) 88
CviAII CATG 5 cut(s) 226, 482, 546, 689, 760
DdeI CTNAG 2 cut(s) 196, 662
DrdI GACNNNNNNGTC 1 cut(s) 482
DseDI GACNNNNNNGTC 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 715
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 717
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 3 cut(s) 254, 602, 631
Eco53kI GAGCTC 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 715
EcoRII CCWGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 5 cut(s) 229, 485, 549, 692, 763
FatI CATG 5 cut(s) 225, 481, 545, 688, 759
FblI GTMKAC 1 cut(s) 685
Fnu4HI GCNGC 2 cut(s) 537, 564
FokI GGATG 3 cut(s) 263, 343, 758
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 2 cut(s) 537, 564
FspBI CTAG 2 cut(s) 317, 609
GluI GCNGC 2 cut(s) 537, 564
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 3 cut(s) 34, 138, 679
HapII CCGG 2 cut(s) 71, 676
HgaI GACGC 1 cut(s) 88
Hin1II CATG 5 cut(s) 229, 485, 549, 692, 763
HinfI GANTC 1 cut(s) 409
HpaII CCGG 2 cut(s) 71, 676
HphI GGTGA 1 cut(s) 601
Hpy166II GTNNAC 6 cut(s) 82, 467, 592, 631, 686, 736
Hpy188I TCNGA 3 cut(s) 237, 325, 361
Hpy188III TCNNGA 4 cut(s) 413, 464, 473, 671
Hpy8I GTNNAC 6 cut(s) 82, 467, 592, 631, 686, 736
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 2 cut(s) 241, 536
HpyCH4III ACNGT 1 cut(s) 264
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 3 cut(s) 505, 545, 563
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 542, 551, 560
HpyF3I CTNAG 2 cut(s) 196, 662
HpySE526I ACGT 1 cut(s) 445
Hsp92II CATG 5 cut(s) 229, 485, 549, 692, 763
LmnI GCTCC 3 cut(s) 72, 497, 712
Lsp1109I GCAGC 2 cut(s) 523, 575
LweI GCATC 3 cut(s) 329, 505, 514
MaeI CTAG 2 cut(s) 317, 609
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 3 cut(s) 220, 268, 755
MboII GAAGA 1 cut(s) 121
MfeI CAATTG 2 cut(s) 613, 644
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 6 cut(s) 437, 510, 557, 613, 644, 697
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 2 cut(s) 72, 215
MnlI CCTC 5 cut(s) 32, 444, 471, 480, 497
MseI TTAA 1 cut(s) 125
MspI CCGG 2 cut(s) 71, 676
MspR9I CCNGG 2 cut(s) 71, 212
MunI CAATTG 2 cut(s) 613, 644
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 4 cut(s) 64, 542, 551, 560
NciI CCSGG 1 cut(s) 71
NcoI CCATGG 1 cut(s) 225
NlaIII CATG 5 cut(s) 229, 485, 549, 692, 763
NlaIV GGNNCC 4 cut(s) 68, 137, 256, 432
NspI RCATGY 3 cut(s) 485, 692, 763
NspV TTCGAA 1 cut(s) 529
PciI ACATGT 2 cut(s) 688, 759
PflMI CCANNNNNTGG 3 cut(s) 41, 264, 546
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 2 cut(s) 538, 565
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
Ppu21I YACGTR 1 cut(s) 446
PscI ACATGT 2 cut(s) 688, 759
PsiI TTATAA 1 cut(s) 704
Psp124BI GAGCTC 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 4 cut(s) 68, 137, 256, 432
PspPI GGNCC 5 cut(s) 136, 254, 602, 631, 678
SacI GAGCTC 1 cut(s) 717
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 2 cut(s) 537, 564
Sau96I GGNCC 5 cut(s) 136, 254, 602, 631, 678
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 2 cut(s) 71, 212
SduI GDGCHC 1 cut(s) 717
SfaNI GCATC 3 cut(s) 329, 505, 514
SfuI TTCGAA 1 cut(s) 529
SinI GGWCC 3 cut(s) 254, 602, 631
Sse9I AATT 6 cut(s) 437, 510, 557, 613, 644, 697
SspMI CTAG 2 cut(s) 317, 609
SstI GAGCTC 1 cut(s) 717
StyD4I CCNGG 2 cut(s) 69, 210
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 264
TaiI ACGT 1 cut(s) 448
TaqI TCGA 1 cut(s) 529
TasI AATT 6 cut(s) 437, 510, 557, 613, 644, 697
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 3 cut(s) 68, 181, 547
TseI GCWGC 2 cut(s) 536, 563
TspRI CASTG 3 cut(s) 68, 181, 547
Van91I CCANNNNNTGG 3 cut(s) 41, 264, 546
VpaK11BI GGWCC 3 cut(s) 254, 602, 631
XapI RAATTY 1 cut(s) 557
XceI RCATGY 3 cut(s) 485, 692, 763
XmiI GTMKAC 1 cut(s) 685
XspI CTAG 2 cut(s) 317, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.