Rh5AG309700

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005245
Physical Location & Seq
Reverse (-)
44442808 .. 44450455
7648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG309700.1

Sequence Viewer

Length: 1119 bp
ATGAGGCCGATACGTCTTCCTGAGCCGCCCAGCCCCAAAATGGGGACGCCGGAAATCTTCGAAGGCGGCGTGTACGGCGTCGTACGCCGCGCCGTCGTGATTGGCAACGGCTTCCCCGGTTCCGAGAATCAGAGTATCGGCCTGGTTCGCGCTCTCGGCCTCGCCGACAAGCACGTCGTGTATCGAGTTACAAGGCCAAGAGGAGGTCTAAATGAGTGGCTTCACTGGCTCCCGGTTTCTGTTCACAAGAAGTTGGATTGTGTCATAAGGAAGATGTGTATTTTGTTAATATATCGGGGGCAGAAAGTTGGGTCTCTACCAACAGAAAATGGTGGCAGTGCTGGGTTGAAATGTGTCTTAGAAGCTGATGTTCAGGAGATTGTAACCATGGCTCGTCGGACTTATGAAAAGGATGGACCCATACTGGTGGTAGCTTGTGGCAGGGATACCATTTCCATTGCAAGTTCTATTAGACGACTAGCATCTGAAAACGTCTTTGTTGTTCAGATACAACATCCCAGGTTGCATCTCAATAGATTTGACTTGGTTATTACCCCGAAACACGATTACTATCCTTTGACCCCTGAAGCACAGAAGCAGGTTCCTCAATTTCTACGTGGGTGGATAACTCCTCGTGAGCCTCCTGACAGGCATGTGGTCCTCACTATTGGAGCTTTGCATCAAATTGATGCTACTGCCCTTCGTAATGCTGCCAGTGCATGGCGTGATGAATTTGCAGCTCTACCAAAGCCCTTACTTGTGGTGAACATTGGTGGTCCTACAAGCAATTGTCGTTATGGTGTGGACCTTGCGAAGCAATTGGCAACCTATTTGCTTAGTGTTCTTGTAAGCTGTGGAAGCATCAGAATATGTTTCTCTAGTAGAACACCTGAAAAGGTATCCAATGTCATAACAAAGGAGCTCGGAGGTAATCCAAAAGTTTACATTTGGGATGGTCAAGAGCCAAACCCGCACATGGGCCATCTGGCCTGGGCCGATGCGTTTGTTGTCACTGCAGATTCCGTTAGCATGATAAGTGAGGTTTGCAGCACCGGGTATGGTCGAGAAATTATCTCTATAACTGATGACTGGGGACTTATTTTCTTCCATTTTATTTAA

Protein Analysis

372

Amino Acids

41.13

Weight (kDa)

8.97

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 39 - 352 1.9e-86 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 589
AccB7I CCANNNNNTGG 1 cut(s) 720
AccII CGCG 2 cut(s) 90, 150
AciI CCGC 4 cut(s) 26, 66, 88, 971
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 1 cut(s) 606
AcyI GRCGYC 2 cut(s) 47, 78
AdeI CACNNNGTG 1 cut(s) 178
AfaI GTAC 2 cut(s) 74, 84
AfiI CCNNNNNNNGG 7 cut(s) 40, 41, 42, 203, 720, 976, 977
AgsI TTSAA 1 cut(s) 349
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 3 cut(s) 141, 518, 989
AluBI AGCT 6 cut(s) 365, 434, 674, 740, 852, 922
AluI AGCT 6 cut(s) 365, 434, 674, 740, 852, 922
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 1 cut(s) 318
AoxI GGCC 7 cut(s) 5, 139, 157, 194, 979, 987, 993
ApeKI GCWGC 3 cut(s) 710, 737, 1047
ApoI RAATTY 1 cut(s) 731
AspLEI GCGC 2 cut(s) 92, 152
AspS9I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
AsuC2I CCSGG 3 cut(s) 117, 233, 1054
AsuHPI GGTGA 1 cut(s) 775
AsuII TTCGAA 1 cut(s) 60
AvaII GGWCC 4 cut(s) 416, 658, 776, 805
BanII GRGCYC 1 cut(s) 924
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 3 cut(s) 697, 749, 1059
BccI CCATC 3 cut(s) 407, 947, 990
BceAI ACGGC 3 cut(s) 77, 91, 124
BciT130I CCWGG 3 cut(s) 143, 520, 991
BciVI GTATCC 2 cut(s) 439, 910
BcnI CCSGG 3 cut(s) 117, 233, 1054
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 2 cut(s) 479, 879
BfmI CTRYAG 1 cut(s) 1014
BfuAI ACCTGC 1 cut(s) 589
BfuI GTATCC 2 cut(s) 439, 910
BisI GCNGC 6 cut(s) 26, 67, 88, 711, 738, 1048
BlsI GCNGC 6 cut(s) 27, 68, 89, 712, 739, 1049
Bme1390I CCNGG 6 cut(s) 117, 143, 233, 520, 991, 1054
Bme18I GGWCC 4 cut(s) 416, 658, 776, 805
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
BmiI GGNNCC 4 cut(s) 121, 230, 418, 603
BmrFI CCNGG 6 cut(s) 117, 143, 233, 520, 991, 1054
BmrI ACTGGG 1 cut(s) 1099
BmsI GCATC 6 cut(s) 491, 535, 679, 688, 870, 988
BmuI ACTGGG 1 cut(s) 1099
BpiI GAAGAC 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 60
BpuMI CCSGG 3 cut(s) 117, 233, 1054
BsaAI YACGTR 1 cut(s) 617
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 2 cut(s) 47, 78
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 115, 387, 518, 990
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 41, 42, 203, 720, 976, 977
Bse1I ACTGG 4 cut(s) 230, 429, 714, 1094
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 3 cut(s) 143, 520, 991
BseDI CCNNGG 4 cut(s) 115, 387, 518, 990
BseGI GGATG 3 cut(s) 418, 514, 958
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 7 cut(s) 40, 41, 42, 203, 720, 976, 977
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 4 cut(s) 230, 429, 714, 1094
BseRI GAGGAG 2 cut(s) 216, 621
BseXI GCAGC 3 cut(s) 697, 749, 1059
BseYI CCCAGC 2 cut(s) 29, 341
Bsh1236I CGCG 2 cut(s) 90, 150
BshFI GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 995
BsiHKAI GWGCWC 1 cut(s) 924
BsiSI CCGG 4 cut(s) 50, 117, 233, 1053
BsiWI CGTACG 1 cut(s) 82
BslFI GGGAC 2 cut(s) 58, 1107
BslI CCNNNNNNNGG 7 cut(s) 40, 41, 42, 203, 720, 976, 977
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 2 cut(s) 58, 1107
BsnI GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 995
Bso31I GGTCTC 1 cut(s) 318
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 924
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 4 cut(s) 26, 66, 88, 971
BspANI GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 995
BspCNI CTCAG 1 cut(s) 13
BspFNI CGCG 2 cut(s) 90, 150
BspLI GGNNCC 4 cut(s) 121, 230, 418, 603
BspMAI CTGCAG 1 cut(s) 1018
BspMI ACCTGC 1 cut(s) 589
BspT104I TTCGAA 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 318
BsrDI GCAATG 1 cut(s) 456
BsrI ACTGG 4 cut(s) 230, 429, 714, 1094
BssECI CCNNGG 4 cut(s) 115, 387, 518, 990
BssNI GRCGYC 2 cut(s) 47, 78
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 3 cut(s) 143, 520, 991
BstACI GRCGYC 2 cut(s) 47, 78
BstBAI YACGTR 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 60
BstDEI CTNAG 3 cut(s) 21, 358, 836
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 418, 514, 958
BstFNI CGCG 2 cut(s) 90, 150
BstHHI GCGC 2 cut(s) 92, 152
BstMAI GTCTC 1 cut(s) 318
BstMWI GCNNNNNNNGC 8 cut(s) 75, 84, 147, 156, 226, 716, 858, 970
BstNI CCWGG 3 cut(s) 143, 520, 991
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 6 cut(s) 115, 141, 231, 518, 989, 1052
BstSFI CTRYAG 1 cut(s) 1014
BstUI CGCG 2 cut(s) 90, 150
BstV1I GCAGC 3 cut(s) 697, 749, 1059
BstV2I GAAGAC 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 427
BsuI GTATCC 2 cut(s) 439, 910
BsuRI GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 995
BtgI CCRYGG 1 cut(s) 387
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 3 cut(s) 418, 514, 958
BtsI GCAGTG 2 cut(s) 343, 1011
BtsIMutI CAGTG 4 cut(s) 223, 343, 721, 1011
BveI ACCTGC 1 cut(s) 589
CfoI GCGC 2 cut(s) 92, 152
Cfr13I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
CseI GACGC 2 cut(s) 55, 67
Csp6I GTAC 2 cut(s) 73, 83
CviAII CATG 5 cut(s) 388, 653, 720, 976, 1030
CviQI GTAC 2 cut(s) 73, 83
DdeI CTNAG 3 cut(s) 21, 358, 836
DraIII CACNNNGTG 1 cut(s) 178
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
Ecl136II GAGCTC 1 cut(s) 922
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 1 cut(s) 318
Eco47I GGWCC 4 cut(s) 416, 658, 776, 805
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 606
EcoICRI GAGCTC 1 cut(s) 922
EcoRII CCWGG 3 cut(s) 141, 518, 989
EcoT14I CCWWGG 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 391, 656, 723, 979, 1033
FaqI GGGAC 2 cut(s) 58, 1107
FatI CATG 5 cut(s) 387, 652, 719, 975, 1029
FauI CCCGC 1 cut(s) 978
Fnu4HI GCNGC 6 cut(s) 26, 67, 88, 711, 738, 1048
FokI GGATG 3 cut(s) 425, 501, 965
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 6 cut(s) 26, 67, 88, 711, 738, 1048
FspBI CTAG 2 cut(s) 479, 879
GlaI GCGC 2 cut(s) 91, 151
GluI GCNGC 6 cut(s) 26, 67, 88, 711, 738, 1048
GsaI CCCAGC 2 cut(s) 33, 345
HaeIII GGCC 7 cut(s) 7, 141, 159, 196, 981, 989, 995
HapII CCGG 4 cut(s) 50, 117, 233, 1053
HgaI GACGC 2 cut(s) 55, 67
HhaI GCGC 2 cut(s) 92, 152
Hin1I GRCGYC 2 cut(s) 47, 78
Hin1II CATG 5 cut(s) 391, 656, 723, 979, 1033
Hin6I GCGC 2 cut(s) 90, 150
HinP1I GCGC 2 cut(s) 90, 150
HinfI GANTC 2 cut(s) 127, 1019
HpaII CCGG 4 cut(s) 50, 117, 233, 1053
HphI GGTGA 1 cut(s) 775
Hpy166II GTNNAC 5 cut(s) 73, 244, 766, 805, 943
Hpy188I TCNGA 7 cut(s) 124, 132, 399, 487, 507, 866, 926
Hpy188III TCNNGA 7 cut(s) 20, 97, 374, 635, 644, 959, 1064
Hpy8I GTNNAC 5 cut(s) 73, 244, 766, 805, 943
Hpy99I CGWCG 4 cut(s) 83, 98, 179, 399
HpyAV CCTTC 2 cut(s) 56, 710
HpyCH4IV ACGT 4 cut(s) 13, 174, 492, 616
HpyCH4V TGCA 7 cut(s) 461, 526, 679, 719, 737, 1016, 1047
HpyF10VI GCNNNNNNNGC 8 cut(s) 75, 84, 147, 156, 226, 716, 858, 970
HpyF3I CTNAG 3 cut(s) 21, 358, 836
HpySE526I ACGT 4 cut(s) 13, 174, 492, 616
Hsp92I GRCGYC 2 cut(s) 47, 78
Hsp92II CATG 5 cut(s) 391, 656, 723, 979, 1033
HspAI GCGC 2 cut(s) 90, 150
LmnI GCTCC 3 cut(s) 234, 671, 919
Lsp1109I GCAGC 3 cut(s) 697, 749, 1059
LweI GCATC 6 cut(s) 491, 535, 679, 688, 870, 988
MaeI CTAG 2 cut(s) 479, 879
MaeII ACGT 4 cut(s) 13, 174, 492, 616
MaeIII GTNAC 3 cut(s) 187, 382, 1009
MboII GAAGA 4 cut(s) 8, 49, 283, 1096
MfeI CAATTG 2 cut(s) 787, 818
MhlI GDGCHC 1 cut(s) 924
MluCI AATT 6 cut(s) 608, 684, 731, 787, 818, 1068
MmeI TCCRAC 2 cut(s) 234, 377
MnlI CCTC 9 cut(s) 170, 194, 197, 615, 642, 651, 671, 920, 1033
MseI TTAA 2 cut(s) 287, 1117
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 4 cut(s) 50, 117, 233, 1053
MspR9I CCNGG 6 cut(s) 117, 143, 233, 520, 991, 1054
MunI CAATTG 2 cut(s) 787, 818
MvaI CCWGG 3 cut(s) 143, 520, 991
MvnI CGCG 2 cut(s) 90, 150
MwoI GCNNNNNNNGC 8 cut(s) 75, 84, 147, 156, 226, 716, 858, 970
NciI CCSGG 3 cut(s) 117, 233, 1054
NcoI CCATGG 1 cut(s) 387
NlaIII CATG 5 cut(s) 391, 656, 723, 979, 1033
NlaIV GGNNCC 4 cut(s) 121, 230, 418, 603
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 1009
NspI RCATGY 1 cut(s) 656
NspV TTCGAA 1 cut(s) 60
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 162
PfeI GAWTC 2 cut(s) 127, 1019
Pfl23II CGTACG 1 cut(s) 82
PflMI CCANNNNNTGG 1 cut(s) 720
PkrI GCNGC 6 cut(s) 27, 68, 89, 712, 739, 1049
Ppu21I YACGTR 1 cut(s) 617
Psp124BI GAGCTC 1 cut(s) 924
Psp6I CCWGG 3 cut(s) 141, 518, 989
PspFI CCCAGC 2 cut(s) 29, 341
PspGI CCWGG 3 cut(s) 141, 518, 989
PspLI CGTACG 1 cut(s) 82
PspN4I GGNNCC 4 cut(s) 121, 230, 418, 603
PspPI GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
PstI CTGCAG 1 cut(s) 1018
RsaI GTAC 2 cut(s) 74, 84
RsaNI GTAC 2 cut(s) 73, 83
RseI CAYNNNNRTG 1 cut(s) 425
SacI GAGCTC 1 cut(s) 924
SaqAI TTAA 2 cut(s) 287, 1117
SatI GCNGC 6 cut(s) 26, 67, 88, 711, 738, 1048
Sau96I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
ScrFI CCNGG 6 cut(s) 117, 143, 233, 520, 991, 1054
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 6 cut(s) 491, 535, 679, 688, 870, 988
SfcI CTRYAG 1 cut(s) 1014
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 4 cut(s) 416, 658, 776, 805
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 6 cut(s) 608, 684, 731, 787, 818, 1068
SsiI CCGC 4 cut(s) 26, 66, 88, 971
SspMI CTAG 2 cut(s) 479, 879
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 6 cut(s) 115, 141, 231, 518, 989, 1052
StyI CCWWGG 1 cut(s) 387
TaiI ACGT 4 cut(s) 16, 177, 495, 619
TaqI TCGA 3 cut(s) 60, 184, 1063
TasI AATT 6 cut(s) 608, 684, 731, 787, 818, 1068
TauI GCSGC 3 cut(s) 28, 69, 90
TfiI GAWTC 2 cut(s) 127, 1019
Tru1I TTAA 2 cut(s) 287, 1117
Tru9I TTAA 2 cut(s) 287, 1117
TscAI CASTG 4 cut(s) 230, 343, 721, 1018
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 3 cut(s) 710, 737, 1047
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 2 cut(s) 420, 744
TspGWI ACGGA 1 cut(s) 1012
TspRI CASTG 4 cut(s) 230, 343, 721, 1018
Van91I CCANNNNNTGG 1 cut(s) 720
VpaK11BI GGWCC 4 cut(s) 416, 658, 776, 805
XapI RAATTY 1 cut(s) 731
XceI RCATGY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XspI CTAG 2 cut(s) 479, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.