Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005245

Physical Location & Seq

Genomic Coordinates Forward (+)

16601370 .. 16608531

Gene Structure 7162 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1149300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGAGACCCATACAGCTTCCGGAGCCGCCCAGTCCCAAACTGGGCACACCGGAAATCTTCGAAGGCGGTGTTTACGGCGTCGTTAGGCGCGCCGTCGTGATTGGCAACGGCTACCCTGGTTCGGAGAATCAGAGCATCGGCTTGGTTCGTGCTCTCGGCCTCGCTGATAAGCATGTCTTATATCGTGTTACAAGGCCGAAAGGAGGACTGAATGAGTGGCTTCACTGGCTCCCAGTTTCTCTTCACAAAAACTTAGATTACATCATAAGACAGATTTGTATTATATTAAAGTATCGGGGACAGAGAGGTTTGCCTCTACCTGCAGAAAATGGTGGCGGTGTGGGGTTGAAAGGCGTTTTAGAAGCTGATGTCCATCAGATTGTGACAATGGCTCGTCAGACTTTTGAAAAGGATGGACCTATATTGGTGGTAGCTTCTGGAAGGGATACCATTTCTGTTTCAAGTTCTATTAAACTACTTGCATCTGACAATGCATTTGTTGTTCAGATACTACATCCCAGGTTGCATCTAAATAGATTTGACTTGGTTATTACCCCTAAGCACGATTACTATCCTTTGACTCGTGAAGCACAACAGCAGGTTCCTCAATTTATTCGTAAGTGGATAACTCCACGTGAACCTCCTGACAGTCATGTGGTCCTCACTGTTGGAGCTTTACATCAAGTGGATGCTGGTTCACTTCGTAATGCAGCTAGTGCATGGCGTGATGAGTTTGCACCTTTGCCAAAACCTTTACTTGTGGTGAACATTGGTGGCCCAACAGGCAATTGTCGTTATGGTGCGGACCTTGCAAGGCAATTGACAACCAATCTGCTTAGTGTTCTTGATAGCTGTGGGAGTATCAGAATATGTTTCTCTAATAGGACTCCTGAAAAGGTATCTAATGTTATATTGAAAGAGCTTGGTGGTAATCCAAAAGTTTACATCTGGGATGGTCAAGAGCCAAACCCGCACCTGGGCCATTTAGCCTGGGCCGATGCATTTGTTGTCACTGCAGATTCTGTCAGTATGATAAGCGAGGTTTGCAGCACTGGGAAGCCTGTCTATATTATGGGAGCTGAGCGATGTACATGGAAGTTCTCAGAGTTCCATAAATCACTTAAGGATCGAGGAGTAGTTCGGCCGTTTACAGGTTCTGAGAATATTTCAGAGAGCTGGAGTTACCCTCCGCTCAATGAAACCGCAGAAGCAGCTAGTCGAGTGCACAAAGCACTTGCGGAGCGTGGATGGAGATTGCGACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000266 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006996 GO:0007005 GO:0008150 GO:0009987 GO:0016020 GO:0016043 GO:0019867 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mito_fiss_Elm1

Protein Analysis

422

Amino Acids

46.49

Weight (kDa)

9.22

Isoelectric Point (pI)

31.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mito_fiss_Elm1	PF06258	39 - 412	2.2e-108	Mitochondrial fission ELM1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005245)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22350
fragaria_vesca	FvH4_3g25550 FvH4_3g25550 FvH4_3g25550 FvH4_3g25550 FvH4_3g25550
malus_domestica	MD03G1149300.v1.1 MD11G1168100.v1.1
prunus_persica	Prupe.6G135200_v2.0.a1
pyrus_communis	pycom03g10320 pycom11g14040
rosa_chinensis	RchiOBHm_Chr5g0046451 RchiOBHm_Chr5g0046461 RchiOBHm_Chr5g0046541
rosa_laevigata	RLG00000034412 RLG00000034415
rosa_multiflora	Rmu_sc0003878.1_g000039
rosa_roxburghii	Rroxscaffold_1G00034870
rosa_rugosa	Rorug05G0229100 Rorug05G0229500
rosa_samantha	Rh5AG309700 Rh5BG317700 Rh5BG318100 Rh5CG343700 Rh5DG327900 Rh5DG328100 Rh5DG328400
rosa_wichuraiana	Rw0G010760 Rw5G028880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	328, 589
AccBSI	CCGCTC	1 cut(s)	1192
AccII	CGCG	1 cut(s)	90
AccIII	TCCGGA	1 cut(s)	19
AciI	CCGC	8 cut(s)	26, 66, 336, 803, 971, 1190, 1203, 1238
AclWI	GGATC	1 cut(s)	1134
AcoI	YGGCCR	1 cut(s)	1142
AcvI	CACGTG	1 cut(s)	635
AcyI	GRCGYC	1 cut(s)	78
AfaI	GTAC	1 cut(s)	1090
AfiI	CCNNNNNNNGG	6 cut(s)	41, 121, 203, 976, 977, 1151
AflII	CTTAAG	1 cut(s)	1121
AgsI	TTSAA	4 cut(s)	349, 407, 462, 916
AjnI	CCWGG	4 cut(s)	115, 518, 975, 989
Alw21I	GWGCWC	2 cut(s)	154, 1227
Alw44I	GTGCAC	1 cut(s)	1223
AlwI	GGATC	1 cut(s)	1134
AlwNI	CAGNNNCTG	2 cut(s)	1022, 1157
Aor13HI	TCCGGA	1 cut(s)	19
AoxI	GGCC	6 cut(s)	157, 194, 775, 979, 993, 1142
ApaLI	GTGCAC	1 cut(s)	1223
ApeKI	GCWGC	3 cut(s)	710, 1047, 1211
ArsI	GACNNNNNNTTYG	2 cut(s)	479, 511
AscI	GGCGCGCC	1 cut(s)	88
AspLEI	GCGC	2 cut(s)	90, 92
AspS9I	GGNCC	6 cut(s)	416, 658, 776, 805, 979, 993
AsuHPI	GGTGA	1 cut(s)	775
AsuII	TTCGAA	1 cut(s)	60
AvaII	GGWCC	3 cut(s)	416, 658, 805
BaeGI	GKGCMC	2 cut(s)	47, 1227
BauI	CACGAG	1 cut(s)	582
BbrPI	CACGTG	1 cut(s)	635
Bbv12I	GWGCWC	2 cut(s)	154, 1227
BbvI	GCAGC	3 cut(s)	722, 1059, 1223
BccI	CCATC	4 cut(s)	381, 407, 947, 1242
BceAI	ACGGC	4 cut(s)	77, 91, 124, 1129
BciT130I	CCWGG	4 cut(s)	117, 520, 977, 991
BciVI	GTATCC	1 cut(s)	439
BfaI	CTAG	2 cut(s)	714, 1215
BfmI	CTRYAG	2 cut(s)	321, 1014
BfrI	CTTAAG	1 cut(s)	1121
BfuAI	ACCTGC	2 cut(s)	328, 589
BfuI	GTATCC	1 cut(s)	439
BglI	GCCNNNNNGGC	1 cut(s)	783
BisI	GCNGC	4 cut(s)	26, 711, 1048, 1212
BlpI	GCTNAGC	1 cut(s)	1080
BlsI	GCNGC	4 cut(s)	27, 712, 1049, 1213
Bme1390I	CCNGG	4 cut(s)	117, 520, 977, 991
Bme18I	GGWCC	3 cut(s)	416, 658, 805
BmgT120I	GGNCC	6 cut(s)	416, 658, 776, 805, 979, 993
BmiI	GGNNCC	3 cut(s)	24, 230, 603
BmrFI	CCNGG	4 cut(s)	117, 520, 977, 991
BmrI	ACTGGG	4 cut(s)	24, 50, 227, 1062
BmsI	GCATC	5 cut(s)	144, 491, 535, 679, 988
BmuI	ACTGGG	4 cut(s)	24, 50, 227, 1062
BplI	GAGNNNNNCTC	2 cut(s)	1171, 1203
BpmI	CTGGAG	1 cut(s)	1198
Bpu10I	CCTNAGC	1 cut(s)	558
Bpu1102I	GCTNAGC	1 cut(s)	1080
Bpu14I	TTCGAA	1 cut(s)	60
BsaAI	YACGTR	1 cut(s)	635
BsaBI	GATNNNNATC	2 cut(s)	372, 570
BsaHI	GRCGYC	1 cut(s)	78
BsaJI	CCNNGG	4 cut(s)	115, 518, 976, 990
BsaWI	WCCGGW	2 cut(s)	19, 49
Bsc4I	CCNNNNNNNGG	6 cut(s)	41, 121, 203, 976, 977, 1151
Bse1I	ACTGG	5 cut(s)	30, 45, 230, 233, 1057
Bse8I	GATNNNNATC	2 cut(s)	372, 570
BseAI	TCCGGA	1 cut(s)	19
BseBI	CCWGG	4 cut(s)	117, 520, 977, 991
BseDI	CCNNGG	4 cut(s)	115, 518, 976, 990
BseGI	GGATG	5 cut(s)	418, 514, 694, 958, 1253
BseJI	GATNNNNATC	2 cut(s)	372, 570
BseLI	CCNNNNNNNGG	6 cut(s)	41, 121, 203, 976, 977, 1151
BseMII	CTCAG	3 cut(s)	1071, 1116, 1149
BseNI	ACTGG	5 cut(s)	30, 45, 230, 233, 1057
BsePI	GCGCGC	1 cut(s)	88
BseRI	GAGGAG	1 cut(s)	1146
BseSI	GKGCMC	2 cut(s)	47, 1227
BseX3I	CGGCCG	1 cut(s)	1142
BseXI	GCAGC	3 cut(s)	722, 1059, 1223
Bsh1236I	CGCG	1 cut(s)	90
Bsh1285I	CGRYCG	1 cut(s)	1145
BshFI	GGCC	6 cut(s)	159, 196, 777, 981, 995, 1144
BsiEI	CGRYCG	1 cut(s)	1145
BsiHKAI	GWGCWC	2 cut(s)	154, 1227
BsiSI	CCGG	2 cut(s)	20, 50
BslFI	GGGAC	2 cut(s)	18, 312
BslI	CCNNNNNNNGG	6 cut(s)	41, 121, 203, 976, 977, 1151
BsmFI	GGGAC	2 cut(s)	18, 312
BsnI	GGCC	6 cut(s)	159, 196, 777, 981, 995, 1144
Bsp119I	TTCGAA	1 cut(s)	60
Bsp1286I	GDGCHC	3 cut(s)	47, 154, 1227
Bsp13I	TCCGGA	1 cut(s)	19
Bsp1407I	TGTACA	1 cut(s)	1088
Bsp143I	GATC	1 cut(s)	1126
Bsp1720I	GCTNAGC	1 cut(s)	1080
BspACI	CCGC	8 cut(s)	26, 66, 336, 803, 971, 1190, 1203, 1238
BspANI	GGCC	6 cut(s)	159, 196, 777, 981, 995, 1144
BspCNI	CTCAG	3 cut(s)	1072, 1115, 1150
BspEI	TCCGGA	1 cut(s)	19
BspFNI	CGCG	1 cut(s)	90
BspLI	GGNNCC	3 cut(s)	24, 230, 603
BspMAI	CTGCAG	2 cut(s)	325, 1018
BspMI	ACCTGC	2 cut(s)	328, 589
BspPI	GGATC	1 cut(s)	1134
BspT104I	TTCGAA	1 cut(s)	60
BspTI	CTTAAG	1 cut(s)	1121
BsrBI	CCGCTC	1 cut(s)	1192
BsrGI	TGTACA	1 cut(s)	1088
BsrI	ACTGG	5 cut(s)	30, 45, 230, 233, 1057
BssECI	CCNNGG	4 cut(s)	115, 518, 976, 990
BssHII	GCGCGC	1 cut(s)	88
BssMI	GATC	1 cut(s)	1126
BssNI	GRCGYC	1 cut(s)	78
BssSI	CACGAG	1 cut(s)	582
Bst2BI	CACGAG	1 cut(s)	582
Bst2UI	CCWGG	4 cut(s)	117, 520, 977, 991
Bst4CI	ACNGT	2 cut(s)	650, 667
Bst6I	CTCTTC	1 cut(s)	246
BstACI	GRCGYC	1 cut(s)	78
BstAFI	CTTAAG	1 cut(s)	1121
BstAPI	GCANNNNNTGC	1 cut(s)	716
BstAUI	TGTACA	1 cut(s)	1088
BstBAI	YACGTR	1 cut(s)	635
BstBI	TTCGAA	1 cut(s)	60
BstC8I	GCNNGC	1 cut(s)	90
BstDEI	CTNAG	6 cut(s)	253, 558, 836, 1080, 1102, 1158
BstF5I	GGATG	5 cut(s)	418, 514, 694, 958, 1253
BstFNI	CGCG	1 cut(s)	90
BstHHI	GCGC	2 cut(s)	90, 92
BstKTI	GATC	1 cut(s)	1129
BstMBI	GATC	1 cut(s)	1126
BstMCI	CGRYCG	1 cut(s)	1145
BstMWI	GCNNNNNNNGC	8 cut(s)	22, 226, 716, 783, 809, 970, 1044, 1211
BstNI	CCWGG	4 cut(s)	117, 520, 977, 991
BstNSI	RCATGY	1 cut(s)	176
BstSCI	CCNGG	4 cut(s)	115, 518, 975, 989
BstSFI	CTRYAG	2 cut(s)	321, 1014
BstSLI	GKGCMC	2 cut(s)	47, 1227
BstUI	CGCG	1 cut(s)	90
BstV1I	GCAGC	3 cut(s)	722, 1059, 1223
BstZI	CGGCCG	1 cut(s)	1142
BsuI	GTATCC	1 cut(s)	439
BsuRI	GGCC	6 cut(s)	159, 196, 777, 981, 995, 1144
BtgZI	GCGATG	1 cut(s)	1099
BtsCI	GGATG	5 cut(s)	418, 514, 694, 958, 1253
BtsI	GCAGTG	1 cut(s)	1011
BtsIMutI	CAGTG	4 cut(s)	223, 663, 1011, 1050
BveI	ACCTGC	2 cut(s)	328, 589
Cac8I	GCNNGC	1 cut(s)	90
CaiI	CAGNNNCTG	2 cut(s)	1022, 1157
CfoI	GCGC	2 cut(s)	90, 92
Cfr13I	GGNCC	6 cut(s)	416, 658, 776, 805, 979, 993
CseI	GACGC	1 cut(s)	67
Csp6I	GTAC	1 cut(s)	1089
CviAII	CATG	4 cut(s)	173, 653, 720, 1092
CviQI	GTAC	1 cut(s)	1089
DdeI	CTNAG	6 cut(s)	253, 558, 836, 1080, 1102, 1158
DpnI	GATC	1 cut(s)	1128
DpnII	GATC	1 cut(s)	1126
EaeI	YGGCCR	1 cut(s)	1142
EagI	CGGCCG	1 cut(s)	1142
Eam1104I	CTCTTC	1 cut(s)	246
EarI	CTCTTC	1 cut(s)	246
EclXI	CGGCCG	1 cut(s)	1142
Eco47I	GGWCC	3 cut(s)	416, 658, 805
Eco52I	CGGCCG	1 cut(s)	1142
Eco72I	CACGTG	1 cut(s)	635
EcoRII	CCWGG	4 cut(s)	115, 518, 975, 989
EcoT22I	ATGCAT	2 cut(s)	496, 1003
FaeI	CATG	4 cut(s)	176, 656, 723, 1095
FalI	AAGNNNNNCTT	2 cut(s)	161, 193
FaqI	GGGAC	2 cut(s)	18, 312
FatI	CATG	4 cut(s)	172, 652, 719, 1091
FauI	CCCGC	1 cut(s)	978
Fnu4HI	GCNGC	4 cut(s)	26, 711, 1048, 1212
FokI	GGATG	5 cut(s)	425, 501, 701, 965, 1260
Fsp4HI	GCNGC	4 cut(s)	26, 711, 1048, 1212
FspBI	CTAG	2 cut(s)	714, 1215
GlaI	GCGC	2 cut(s)	89, 91
GluI	GCNGC	4 cut(s)	26, 711, 1048, 1212
GsuI	CTGGAG	1 cut(s)	1198
HaeIII	GGCC	6 cut(s)	159, 196, 777, 981, 995, 1144
HapII	CCGG	2 cut(s)	20, 50
HgaI	GACGC	1 cut(s)	67
HhaI	GCGC	2 cut(s)	90, 92
Hin1I	GRCGYC	1 cut(s)	78
Hin1II	CATG	4 cut(s)	176, 656, 723, 1095
Hin6I	GCGC	2 cut(s)	88, 90
HinP1I	GCGC	2 cut(s)	88, 90
HinfI	GANTC	4 cut(s)	127, 580, 886, 1019
HpaII	CCGG	2 cut(s)	20, 50
HphI	GGTGA	1 cut(s)	775
Hpy166II	GTNNAC	7 cut(s)	73, 638, 698, 766, 943, 1149, 1225
Hpy188III	TCNNGA	8 cut(s)	20, 97, 438, 584, 644, 845, 890, 959
Hpy8I	GTNNAC	7 cut(s)	73, 638, 698, 766, 943, 1149, 1225
Hpy99I	CGWCG	2 cut(s)	83, 98
HpyAV	CCTTC	2 cut(s)	56, 435
HpyCH4III	ACNGT	2 cut(s)	650, 667
HpyCH4IV	ACGT	1 cut(s)	634
HpyF10VI	GCNNNNNNNGC	8 cut(s)	22, 226, 716, 783, 809, 970, 1044, 1211
HpyF3I	CTNAG	6 cut(s)	253, 558, 836, 1080, 1102, 1158
HpySE526I	ACGT	1 cut(s)	634
Hsp92I	GRCGYC	1 cut(s)	78
Hsp92II	CATG	4 cut(s)	176, 656, 723, 1095
HspAI	GCGC	2 cut(s)	88, 90
Kpn2I	TCCGGA	1 cut(s)	19
Kzo9I	GATC	1 cut(s)	1126
LmnI	GCTCC	5 cut(s)	22, 234, 671, 1076, 1240
Lsp1109I	GCAGC	3 cut(s)	722, 1059, 1223
LweI	GCATC	5 cut(s)	144, 491, 535, 679, 988
MaeI	CTAG	2 cut(s)	714, 1215
MaeII	ACGT	1 cut(s)	634
MaeIII	GTNAC	4 cut(s)	187, 382, 1009, 1181
MalI	GATC	1 cut(s)	1128
MbiI	CCGCTC	1 cut(s)	1192
MboI	GATC	1 cut(s)	1126
MboII	GAAGA	2 cut(s)	49, 233
MfeI	CAATTG	2 cut(s)	787, 818
MhlI	GDGCHC	3 cut(s)	47, 154, 1227
MluCI	AATT	3 cut(s)	608, 787, 818
MlyI	GAGTC	2 cut(s)	574, 880
MmeI	TCCRAC	1 cut(s)	649
Mph1103I	ATGCAT	2 cut(s)	496, 1003
MroI	TCCGGA	1 cut(s)	19
MseI	TTAA	3 cut(s)	287, 471, 1122
MspCI	CTTAAG	1 cut(s)	1121
MspI	CCGG	2 cut(s)	20, 50
MspR9I	CCNGG	4 cut(s)	117, 520, 977, 991
MunI	CAATTG	2 cut(s)	787, 818
MvaI	CCWGG	4 cut(s)	117, 520, 977, 991
MvnI	CGCG	1 cut(s)	90
MwoI	GCNNNNNNNGC	8 cut(s)	22, 226, 716, 783, 809, 970, 1044, 1211
NdeII	GATC	1 cut(s)	1126
NlaIII	CATG	4 cut(s)	176, 656, 723, 1095
NlaIV	GGNNCC	3 cut(s)	24, 230, 603
NmeAIII	GCCGAG	1 cut(s)	135
NmuCI	GTSAC	2 cut(s)	382, 1009
NsiI	ATGCAT	2 cut(s)	496, 1003
NspI	RCATGY	1 cut(s)	176
NspV	TTCGAA	1 cut(s)	60
PalAI	GGCGCGCC	1 cut(s)	88
PauI	GCGCGC	1 cut(s)	88
PfeI	GAWTC	2 cut(s)	127, 1019
PkrI	GCNGC	4 cut(s)	27, 712, 1049, 1213
PleI	GAGTC	2 cut(s)	574, 880
PmaCI	CACGTG	1 cut(s)	635
PmlI	CACGTG	1 cut(s)	635
PpsI	GAGTC	2 cut(s)	574, 880
Ppu21I	YACGTR	1 cut(s)	635
Psp6I	CCWGG	4 cut(s)	115, 518, 975, 989
PspCI	CACGTG	1 cut(s)	635
PspGI	CCWGG	4 cut(s)	115, 518, 975, 989
PspN4I	GGNNCC	3 cut(s)	24, 230, 603
PspPI	GGNCC	6 cut(s)	416, 658, 776, 805, 979, 993
PstI	CTGCAG	2 cut(s)	325, 1018
PstNI	CAGNNNCTG	2 cut(s)	1022, 1157
PteI	GCGCGC	1 cut(s)	88
RsaI	GTAC	1 cut(s)	1090
RsaNI	GTAC	1 cut(s)	1089
SaqAI	TTAA	3 cut(s)	287, 471, 1122
SatI	GCNGC	4 cut(s)	26, 711, 1048, 1212
Sau3AI	GATC	1 cut(s)	1126
Sau96I	GGNCC	6 cut(s)	416, 658, 776, 805, 979, 993
SchI	GAGTC	2 cut(s)	574, 880
ScrFI	CCNGG	4 cut(s)	117, 520, 977, 991
SduI	GDGCHC	3 cut(s)	47, 154, 1227
SfaNI	GCATC	5 cut(s)	144, 491, 535, 679, 988
SfcI	CTRYAG	2 cut(s)	321, 1014
SfuI	TTCGAA	1 cut(s)	60
SgsI	GGCGCGCC	1 cut(s)	88
SinI	GGWCC	3 cut(s)	416, 658, 805
SmlI	CTYRAG	1 cut(s)	1121
SmoI	CTYRAG	1 cut(s)	1121
Sse9I	AATT	3 cut(s)	608, 787, 818
SsiI	CCGC	8 cut(s)	26, 66, 336, 803, 971, 1190, 1203, 1238
SspI	AATATT	1 cut(s)	1165
SspMI	CTAG	2 cut(s)	714, 1215
StyD4I	CCNGG	4 cut(s)	115, 518, 975, 989
TaaI	ACNGT	2 cut(s)	650, 667
TaiI	ACGT	1 cut(s)	637
TaqI	TCGA	3 cut(s)	60, 1129, 1219
TasI	AATT	3 cut(s)	608, 787, 818
TatI	WGTACW	1 cut(s)	1088
TauI	GCSGC	1 cut(s)	28
TfiI	GAWTC	2 cut(s)	127, 1019
Tru1I	TTAA	3 cut(s)	287, 471, 1122
Tru9I	TTAA	3 cut(s)	287, 471, 1122
TscAI	CASTG	4 cut(s)	230, 670, 1018, 1057
TseFI	GTSAC	2 cut(s)	382, 1009
TseI	GCWGC	3 cut(s)	710, 1047, 1211
Tsp45I	GTSAC	2 cut(s)	382, 1009
TspDTI	ATGAA	1 cut(s)	1212
TspRI	CASTG	4 cut(s)	230, 670, 1018, 1057
Vha464I	CTTAAG	1 cut(s)	1121
VneI	GTGCAC	1 cut(s)	1223
VpaK11BI	GGWCC	3 cut(s)	416, 658, 805
XceI	RCATGY	1 cut(s)	176
XcmI	CCANNNNNNNNNTGG	1 cut(s)	37
XspI	CTAG	2 cut(s)	714, 1215
Zsp2I	ATGCAT	2 cut(s)	496, 1003

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.