pycom03g10320

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005245
Physical Location & Seq
Forward (+)
9470773 .. 9477412
6640 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g10320.1

Sequence Viewer

Length: 1269 bp
ATGAGACCCATACAGCTTCCGGAGCCGCCCAGTCCCAAACTGGGCACACCGGAAATCTTCGAAGGTGGGGTTTACGGCGTCGTTAGGCGCGCCGTCGTGATTGGCAACGGCTACCCTGGTTCGGAGAATCAGAGCATCGGCTTAGTTCGTGCTCTCGGCCTCGCTGATAAGCATGTCTTATATCGTGTTACAAGGCCGAAAGGAGGACTGAATGAGTGGCTTCACTGGCTCCCAGTTTCTCTTCACAAAAACTTAGATTACATCATAAGACAGATCTGTATTATATTAAAGTATCGGGGGCAGAGACGTTTGCCTCTACCTGCAGAAAATGGTGGATGTGTGGGGTTGAAAGGCGTTTTAGAAGCTGATGTCCATCAGATTGTGACAATGGCTCGTCAGACTTTTGAAAAGGATGGACCTATATTGGTGGTAGCTTCTGGAAGGGATACCATTTCTGTTTCAAGTTCTATTAAACGACTTGCATCTGATAATGCATTTGTTGTTCAGATACTACATCCCAGGTTGCATCTAAATAGATTTGACTTGGTGATTACCCCTAAGCACGATTACTATCCTTTGACTCGTGAAGCACAACAGCAGGTTCCTCAATTTATTTGTAAGTGGATAACTCCACGTGAACCTCCTGACAGTCATGTGGTCCTCACTGTTGGAGCTTTACATCAAGTTGATGCTGGTTCACTTCGTAATGCAGCTAGTGCATGGCGTGATGAGTTTGCACCTTTGCCAAAACCTTTACTTGTGGTGAACATTGGTGGCCCTACAGGCAATTGTCGTTATGGTGCGGACCTTGCAAGGCAATTGACAACCAATCTGCTTAGTGTTCTTGATAGCTGTGGGAGTATCAGAATATGTTTCTCTAATAGGACTCCTGAAAAGGTATCTAATGTTATATCGAAAGAGCTTGGTGGTAATCCAAAAGTTTACATCTGGGATGGTCAAGAGCCAAACCCACACCTGGGCCATTTAGCCTGGGCCGATGCATTTGTTGTCACTGCAGATTCTGTCAGTATGATAAGTGAGGTTTGCAGCACTGGCCTGTCTATATTATCGGAGCTGAGAGATGTAGATGGAAGTTCTCAGAGTTCCATAAATCACTTAAGGATCGATCGAGGAGTAGTTCGGCCGTTTACAGGTTCTGAGAATATTTCAGAGAGCTGGAGTTACCCTCCGCTCAATGAAACCGCAGAAGCAGCCAGTCGAGTGCACAAAGCACTTGCGGAGCGTGGATGGAGATTGCGACCAGAATGA

Protein Analysis

423

Amino Acids

46.48

Weight (kDa)

8.78

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 39 - 412 8.2e-96 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 328, 589
AccBSI CCGCTC 1 cut(s) 1192
AccII CGCG 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 19
AciI CCGC 5 cut(s) 26, 803, 1190, 1203, 1238
AclWI GGATC 1 cut(s) 1130
AcoI YGGCCR 1 cut(s) 1142
AcvI CACGTG 1 cut(s) 635
AcyI GRCGYC 1 cut(s) 78
AfiI CCNNNNNNNGG 6 cut(s) 41, 121, 203, 976, 977, 1151
AflII CTTAAG 1 cut(s) 1117
AgsI TTSAA 3 cut(s) 349, 407, 462
AjnI CCWGG 4 cut(s) 115, 518, 975, 989
AluBI AGCT 9 cut(s) 16, 365, 434, 674, 713, 852, 922, 1075, 1176
AluI AGCT 9 cut(s) 16, 365, 434, 674, 713, 852, 922, 1075, 1176
Alw21I GWGCWC 2 cut(s) 154, 1227
Alw26I GTCTC 1 cut(s) 298
Alw44I GTGCAC 1 cut(s) 1223
AlwI GGATC 1 cut(s) 1130
AlwNI CAGNNNCTG 2 cut(s) 1022, 1157
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 7 cut(s) 157, 194, 775, 979, 993, 1054, 1142
ApaLI GTGCAC 1 cut(s) 1223
ApeKI GCWGC 3 cut(s) 710, 1047, 1211
AscI GGCGCGCC 1 cut(s) 88
AspLEI GCGC 2 cut(s) 90, 92
AspS9I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
AsuHPI GGTGA 2 cut(s) 559, 775
AsuII TTCGAA 1 cut(s) 60
AvaII GGWCC 3 cut(s) 416, 658, 805
BaeGI GKGCMC 2 cut(s) 47, 1227
BauI CACGAG 1 cut(s) 582
BbrPI CACGTG 1 cut(s) 635
Bbv12I GWGCWC 2 cut(s) 154, 1227
BbvI GCAGC 3 cut(s) 722, 1059, 1223
BccI CCATC 5 cut(s) 381, 407, 947, 1082, 1242
BceAI ACGGC 4 cut(s) 77, 91, 124, 1129
BciT130I CCWGG 4 cut(s) 117, 520, 977, 991
BciVI GTATCC 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 1 cut(s) 714
BfmI CTRYAG 3 cut(s) 321, 780, 1014
BfrI CTTAAG 1 cut(s) 1117
BfuAI ACCTGC 2 cut(s) 328, 589
BfuI GTATCC 1 cut(s) 439
BglI GCCNNNNNGGC 1 cut(s) 783
BglII AGATCT 1 cut(s) 273
BisI GCNGC 4 cut(s) 26, 711, 1048, 1212
BlsI GCNGC 4 cut(s) 27, 712, 1049, 1213
Bme1390I CCNGG 4 cut(s) 117, 520, 977, 991
Bme18I GGWCC 3 cut(s) 416, 658, 805
BmgT120I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
BmiI GGNNCC 3 cut(s) 24, 230, 603
BmrFI CCNGG 4 cut(s) 117, 520, 977, 991
BmrI ACTGGG 3 cut(s) 24, 50, 227
BmsI GCATC 5 cut(s) 144, 491, 535, 679, 988
BmuI ACTGGG 3 cut(s) 24, 50, 227
BplI GAGNNNNNCTC 2 cut(s) 1171, 1203
BpmI CTGGAG 1 cut(s) 1198
Bpu10I CCTNAGC 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 60
Bsa29I ATCGAT 1 cut(s) 1125
BsaAI YACGTR 1 cut(s) 635
BsaBI GATNNNNATC 2 cut(s) 372, 570
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 4 cut(s) 115, 518, 976, 990
BsaWI WCCGGW 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 6 cut(s) 41, 121, 203, 976, 977, 1151
Bse1I ACTGG 6 cut(s) 30, 45, 230, 233, 1057, 1215
Bse8I GATNNNNATC 2 cut(s) 372, 570
BseAI TCCGGA 1 cut(s) 19
BseBI CCWGG 4 cut(s) 117, 520, 977, 991
BseCI ATCGAT 1 cut(s) 1125
BseDI CCNNGG 4 cut(s) 115, 518, 976, 990
BseGI GGATG 5 cut(s) 341, 418, 514, 958, 1253
BseJI GATNNNNATC 2 cut(s) 372, 570
BseLI CCNNNNNNNGG 6 cut(s) 41, 121, 203, 976, 977, 1151
BseMII CTCAG 3 cut(s) 1067, 1112, 1149
BseNI ACTGG 6 cut(s) 30, 45, 230, 233, 1057, 1215
BsePI GCGCGC 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 1146
BseSI GKGCMC 2 cut(s) 47, 1227
BseX3I CGGCCG 1 cut(s) 1142
BseXI GCAGC 3 cut(s) 722, 1059, 1223
Bsh1236I CGCG 1 cut(s) 90
Bsh1285I CGRYCG 2 cut(s) 1129, 1145
BshFI GGCC 7 cut(s) 159, 196, 777, 981, 995, 1056, 1144
BshVI ATCGAT 1 cut(s) 1125
BsiEI CGRYCG 2 cut(s) 1129, 1145
BsiHKAI GWGCWC 2 cut(s) 154, 1227
BsiSI CCGG 2 cut(s) 20, 50
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 6 cut(s) 41, 121, 203, 976, 977, 1151
BsmAI GTCTC 1 cut(s) 298
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 7 cut(s) 159, 196, 777, 981, 995, 1056, 1144
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 3 cut(s) 47, 154, 1227
Bsp13I TCCGGA 1 cut(s) 19
Bsp143I GATC 3 cut(s) 273, 1122, 1126
BspACI CCGC 5 cut(s) 26, 803, 1190, 1203, 1238
BspANI GGCC 7 cut(s) 159, 196, 777, 981, 995, 1056, 1144
BspCNI CTCAG 3 cut(s) 1068, 1111, 1150
BspDI ATCGAT 1 cut(s) 1125
BspEI TCCGGA 1 cut(s) 19
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 3 cut(s) 24, 230, 603
BspMAI CTGCAG 2 cut(s) 325, 1018
BspMI ACCTGC 2 cut(s) 328, 589
BspPI GGATC 1 cut(s) 1130
BspT104I TTCGAA 1 cut(s) 60
BspTI CTTAAG 1 cut(s) 1117
BsrBI CCGCTC 1 cut(s) 1192
BsrI ACTGG 6 cut(s) 30, 45, 230, 233, 1057, 1215
BssECI CCNNGG 4 cut(s) 115, 518, 976, 990
BssHII GCGCGC 1 cut(s) 88
BssMI GATC 3 cut(s) 273, 1122, 1126
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 4 cut(s) 117, 520, 977, 991
Bst4CI ACNGT 2 cut(s) 650, 667
Bst6I CTCTTC 1 cut(s) 246
BstACI GRCGYC 1 cut(s) 78
BstAFI CTTAAG 1 cut(s) 1117
BstAPI GCANNNNNTGC 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 7 cut(s) 142, 253, 558, 836, 1076, 1098, 1158
BstF5I GGATG 5 cut(s) 341, 418, 514, 958, 1253
BstFNI CGCG 1 cut(s) 90
BstHHI GCGC 2 cut(s) 90, 92
BstKTI GATC 3 cut(s) 276, 1125, 1129
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 3 cut(s) 273, 1122, 1126
BstMCI CGRYCG 2 cut(s) 1129, 1145
BstMWI GCNNNNNNNGC 7 cut(s) 22, 226, 716, 783, 809, 1053, 1211
BstNI CCWGG 4 cut(s) 117, 520, 977, 991
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 4 cut(s) 115, 518, 975, 989
BstSFI CTRYAG 3 cut(s) 321, 780, 1014
BstSLI GKGCMC 2 cut(s) 47, 1227
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 722, 1059, 1223
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BstZI CGGCCG 1 cut(s) 1142
Bsu15I ATCGAT 1 cut(s) 1125
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 7 cut(s) 159, 196, 777, 981, 995, 1056, 1144
BsuTUI ATCGAT 1 cut(s) 1125
BtsCI GGATG 5 cut(s) 341, 418, 514, 958, 1253
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 4 cut(s) 223, 663, 1011, 1050
BveI ACCTGC 2 cut(s) 328, 589
Cac8I GCNNGC 1 cut(s) 90
CaiI CAGNNNCTG 2 cut(s) 1022, 1157
CfoI GCGC 2 cut(s) 90, 92
Cfr13I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
ClaI ATCGAT 1 cut(s) 1125
CseI GACGC 1 cut(s) 67
CviAII CATG 3 cut(s) 173, 653, 720
DdeI CTNAG 7 cut(s) 142, 253, 558, 836, 1076, 1098, 1158
DpnI GATC 3 cut(s) 275, 1124, 1128
DpnII GATC 3 cut(s) 273, 1122, 1126
EaeI YGGCCR 1 cut(s) 1142
EagI CGGCCG 1 cut(s) 1142
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
EclXI CGGCCG 1 cut(s) 1142
Eco47I GGWCC 3 cut(s) 416, 658, 805
Eco52I CGGCCG 1 cut(s) 1142
Eco72I CACGTG 1 cut(s) 635
EcoRII CCWGG 4 cut(s) 115, 518, 975, 989
EcoT22I ATGCAT 2 cut(s) 496, 1003
Esp3I CGTCTC 1 cut(s) 298
FaeI CATG 3 cut(s) 176, 656, 723
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 172, 652, 719
Fnu4HI GCNGC 4 cut(s) 26, 711, 1048, 1212
FokI GGATG 5 cut(s) 348, 425, 501, 965, 1260
Fsp4HI GCNGC 4 cut(s) 26, 711, 1048, 1212
FspBI CTAG 1 cut(s) 714
GlaI GCGC 2 cut(s) 89, 91
GluI GCNGC 4 cut(s) 26, 711, 1048, 1212
GsuI CTGGAG 1 cut(s) 1198
HaeIII GGCC 7 cut(s) 159, 196, 777, 981, 995, 1056, 1144
HapII CCGG 2 cut(s) 20, 50
HgaI GACGC 1 cut(s) 67
HhaI GCGC 2 cut(s) 90, 92
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 3 cut(s) 176, 656, 723
Hin6I GCGC 2 cut(s) 88, 90
HinP1I GCGC 2 cut(s) 88, 90
HinfI GANTC 4 cut(s) 127, 580, 886, 1019
HpaII CCGG 2 cut(s) 20, 50
HphI GGTGA 2 cut(s) 559, 775
Hpy166II GTNNAC 7 cut(s) 73, 638, 698, 766, 943, 1149, 1225
Hpy188III TCNNGA 8 cut(s) 20, 97, 438, 584, 644, 845, 890, 959
Hpy8I GTNNAC 7 cut(s) 73, 638, 698, 766, 943, 1149, 1225
Hpy99I CGWCG 2 cut(s) 83, 98
HpyAV CCTTC 2 cut(s) 56, 435
HpyCH4III ACNGT 2 cut(s) 650, 667
HpyCH4IV ACGT 2 cut(s) 307, 634
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 226, 716, 783, 809, 1053, 1211
HpyF3I CTNAG 7 cut(s) 142, 253, 558, 836, 1076, 1098, 1158
HpySE526I ACGT 2 cut(s) 307, 634
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 3 cut(s) 176, 656, 723
HspAI GCGC 2 cut(s) 88, 90
Kpn2I TCCGGA 1 cut(s) 19
Kzo9I GATC 3 cut(s) 273, 1122, 1126
LmnI GCTCC 5 cut(s) 22, 234, 671, 1072, 1240
Lsp1109I GCAGC 3 cut(s) 722, 1059, 1223
LweI GCATC 5 cut(s) 144, 491, 535, 679, 988
MaeI CTAG 1 cut(s) 714
MaeII ACGT 2 cut(s) 307, 634
MaeIII GTNAC 4 cut(s) 187, 382, 1009, 1181
MalI GATC 3 cut(s) 275, 1124, 1128
MbiI CCGCTC 1 cut(s) 1192
MboI GATC 3 cut(s) 273, 1122, 1126
MboII GAAGA 2 cut(s) 49, 233
MfeI CAATTG 2 cut(s) 787, 818
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 3 cut(s) 47, 154, 1227
MluCI AATT 3 cut(s) 608, 787, 818
MlyI GAGTC 2 cut(s) 574, 880
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 9 cut(s) 170, 197, 324, 615, 651, 671, 1033, 1124, 1197
Mph1103I ATGCAT 2 cut(s) 496, 1003
MroI TCCGGA 1 cut(s) 19
MseI TTAA 3 cut(s) 287, 471, 1118
MspCI CTTAAG 1 cut(s) 1117
MspI CCGG 2 cut(s) 20, 50
MspR9I CCNGG 4 cut(s) 117, 520, 977, 991
MunI CAATTG 2 cut(s) 787, 818
MvaI CCWGG 4 cut(s) 117, 520, 977, 991
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 7 cut(s) 22, 226, 716, 783, 809, 1053, 1211
NdeII GATC 3 cut(s) 273, 1122, 1126
NlaIII CATG 3 cut(s) 176, 656, 723
NlaIV GGNNCC 3 cut(s) 24, 230, 603
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 2 cut(s) 382, 1009
NsiI ATGCAT 2 cut(s) 496, 1003
NspI RCATGY 1 cut(s) 176
NspV TTCGAA 1 cut(s) 60
PalAI GGCGCGCC 1 cut(s) 88
PauI GCGCGC 1 cut(s) 88
PfeI GAWTC 2 cut(s) 127, 1019
PkrI GCNGC 4 cut(s) 27, 712, 1049, 1213
Ple19I CGATCG 1 cut(s) 1129
PleI GAGTC 2 cut(s) 574, 880
PmaCI CACGTG 1 cut(s) 635
PmlI CACGTG 1 cut(s) 635
PpsI GAGTC 2 cut(s) 574, 880
Ppu21I YACGTR 1 cut(s) 635
Psp6I CCWGG 4 cut(s) 115, 518, 975, 989
PspCI CACGTG 1 cut(s) 635
PspGI CCWGG 4 cut(s) 115, 518, 975, 989
PspN4I GGNNCC 3 cut(s) 24, 230, 603
PspPI GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
PstI CTGCAG 2 cut(s) 325, 1018
PstNI CAGNNNCTG 2 cut(s) 1022, 1157
PsuI RGATCY 1 cut(s) 273
PteI GCGCGC 1 cut(s) 88
PvuI CGATCG 1 cut(s) 1129
SaqAI TTAA 3 cut(s) 287, 471, 1118
SatI GCNGC 4 cut(s) 26, 711, 1048, 1212
Sau3AI GATC 3 cut(s) 273, 1122, 1126
Sau96I GGNCC 6 cut(s) 416, 658, 776, 805, 979, 993
SchI GAGTC 2 cut(s) 574, 880
ScrFI CCNGG 4 cut(s) 117, 520, 977, 991
SduI GDGCHC 3 cut(s) 47, 154, 1227
SfaNI GCATC 5 cut(s) 144, 491, 535, 679, 988
SfcI CTRYAG 3 cut(s) 321, 780, 1014
SfuI TTCGAA 1 cut(s) 60
SgsI GGCGCGCC 1 cut(s) 88
SinI GGWCC 3 cut(s) 416, 658, 805
SmlI CTYRAG 1 cut(s) 1117
SmoI CTYRAG 1 cut(s) 1117
Sse9I AATT 3 cut(s) 608, 787, 818
SsiI CCGC 5 cut(s) 26, 803, 1190, 1203, 1238
SspI AATATT 1 cut(s) 1165
SspMI CTAG 1 cut(s) 714
StyD4I CCNGG 4 cut(s) 115, 518, 975, 989
TaaI ACNGT 2 cut(s) 650, 667
TaiI ACGT 2 cut(s) 310, 637
TaqI TCGA 5 cut(s) 60, 914, 1125, 1129, 1219
TasI AATT 3 cut(s) 608, 787, 818
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 2 cut(s) 127, 1019
Tru1I TTAA 3 cut(s) 287, 471, 1118
Tru9I TTAA 3 cut(s) 287, 471, 1118
TscAI CASTG 4 cut(s) 230, 670, 1018, 1057
TseFI GTSAC 2 cut(s) 382, 1009
TseI GCWGC 3 cut(s) 710, 1047, 1211
Tsp45I GTSAC 2 cut(s) 382, 1009
TspDTI ATGAA 1 cut(s) 1212
TspRI CASTG 4 cut(s) 230, 670, 1018, 1057
Vha464I CTTAAG 1 cut(s) 1117
VneI GTGCAC 1 cut(s) 1223
VpaK11BI GGWCC 3 cut(s) 416, 658, 805
XceI RCATGY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XspI CTAG 1 cut(s) 714
Zsp2I ATGCAT 2 cut(s) 496, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.