Rh5DG328400

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005245
Physical Location & Seq
Reverse (-)
43186302 .. 43193875
7574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG328400.1

Sequence Viewer

Length: 897 bp
ATGAGGCCGATACGTCTTCCTGAGCCGCCCAGCCCCAAAATGGGGACGCCGGAAATCTTCGAAGGCGGCGTATACGGCGTCGTACGCCGCGCCGTCGTGATTGGCAACGGCTTCCCCGGTTCCGAGAATCAGAGTATCGGCCTGGTTCGCGCTCTCGGCCTCGCCGACAAGCACGTCGTGTATCGAGTTACAAGGCCAAGAGGAGGTCTAAATGAGTGGCTTCACTGGCTCCCGGTTTCTGTTCACAAGAAGTTGGATTGTGTCATAAGGAAGATGTGTATTTTGTTAATATATCGGGGGCAGAAAGTTGGGTCTCTACCAACAGAAAATGGTGGCAGTGCTGGGTTGAAATGTGTCTTAGAAGCTGATGTCCAGGAGATTGTAACCATGGCTCGTCAGACTTATGAAAAGGATGGACCCATACTGGTGGTAGCTTGTGGCAGGGATACTATTTCCATTGCAAGTTCTCTTAGACGACTAGCATCTGAAAACGTCTTTGTTGTACAGATACAACATCCTAGGTTGCATCTCAATAGATTTGACTTGGTTGTTACCCCGAAACACGATTACTATCCTTTGACCCCTGAAGCACAGAAGCAGGTTCCTCAATTTCTACGTGGGTGGATAACTCCTCGTGAACCTCCTGACAGGCATGTGGTCCTCACTATTGGAGCTTTGCATCAAATTGATGCTACTGCCCTTCGTAATGCTGCCAGTGCATGGCGTGATGAATTTGCAGCTCTACCAAAGCCCTTACTTGTGGCGAACATTGGTGGTCCTACAAGTAATCTTTTGTCATACACTGAATCTTTGTTGCTTGTTACTTATAGGATTGGCATCAAAGTATTTGATTACCGAGCATGCTTGCTAGTTGCTGTTATTCTGTTTTTGAATTAA

Protein Analysis

298

Amino Acids

33.05

Weight (kDa)

9.51

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 39 - 264 5.7e-47 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 589
AccB7I CCANNNNNTGG 1 cut(s) 720
AccI GTMKAC 1 cut(s) 72
AccII CGCG 2 cut(s) 90, 150
AciI CCGC 3 cut(s) 26, 66, 88
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 1 cut(s) 606
AcyI GRCGYC 2 cut(s) 47, 78
AdeI CACNNNGTG 1 cut(s) 178
AfaI GTAC 2 cut(s) 84, 504
AfiI CCNNNNNNNGG 5 cut(s) 40, 41, 42, 203, 720
AgsI TTSAA 2 cut(s) 349, 892
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 2 cut(s) 141, 372
AluBI AGCT 4 cut(s) 365, 434, 674, 740
AluI AGCT 4 cut(s) 365, 434, 674, 740
Alw26I GTCTC 1 cut(s) 318
AoxI GGCC 4 cut(s) 5, 139, 157, 194
ApeKI GCWGC 2 cut(s) 710, 737
ApoI RAATTY 1 cut(s) 731
AspA2I CCTAGG 1 cut(s) 518
AspLEI GCGC 2 cut(s) 92, 152
AspS9I GGNCC 3 cut(s) 416, 658, 776
AsuC2I CCSGG 2 cut(s) 117, 233
AsuII TTCGAA 1 cut(s) 60
AvaII GGWCC 3 cut(s) 416, 658, 776
AvrII CCTAGG 1 cut(s) 518
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 1 cut(s) 8
BbvI GCAGC 2 cut(s) 697, 749
BccI CCATC 1 cut(s) 407
BceAI ACGGC 3 cut(s) 77, 91, 124
BciT130I CCWGG 2 cut(s) 143, 374
BciVI GTATCC 1 cut(s) 439
BcnI CCSGG 2 cut(s) 117, 233
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 3 cut(s) 479, 519, 869
BfuAI ACCTGC 1 cut(s) 589
BfuI GTATCC 1 cut(s) 439
BisI GCNGC 5 cut(s) 26, 67, 88, 711, 738
BlnI CCTAGG 1 cut(s) 518
BlsI GCNGC 5 cut(s) 27, 68, 89, 712, 739
Bme1390I CCNGG 4 cut(s) 117, 143, 233, 374
Bme18I GGWCC 3 cut(s) 416, 658, 776
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 3 cut(s) 416, 658, 776
BmiI GGNNCC 4 cut(s) 121, 230, 418, 603
BmrFI CCNGG 4 cut(s) 117, 143, 233, 374
BmsI GCATC 5 cut(s) 491, 535, 679, 688, 846
BpiI GAAGAC 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 60
BpuMI CCSGG 2 cut(s) 117, 233
BsaAI YACGTR 1 cut(s) 617
BsaBI GATNNNNATC 2 cut(s) 570, 836
BsaHI GRCGYC 2 cut(s) 47, 78
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 115, 387, 518
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 41, 42, 203, 720
Bse1I ACTGG 3 cut(s) 230, 429, 714
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 2 cut(s) 570, 836
BseBI CCWGG 2 cut(s) 143, 374
BseDI CCNNGG 3 cut(s) 115, 387, 518
BseGI GGATG 2 cut(s) 418, 514
BseJI GATNNNNATC 2 cut(s) 570, 836
BseLI CCNNNNNNNGG 5 cut(s) 40, 41, 42, 203, 720
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 3 cut(s) 230, 429, 714
BseRI GAGGAG 2 cut(s) 216, 621
BseXI GCAGC 2 cut(s) 697, 749
BseYI CCCAGC 2 cut(s) 29, 341
Bsh1236I CGCG 2 cut(s) 90, 150
BshFI GGCC 4 cut(s) 7, 141, 159, 196
BsiSI CCGG 3 cut(s) 50, 117, 233
BsiWI CGTACG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 5 cut(s) 40, 41, 42, 203, 720
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 4 cut(s) 7, 141, 159, 196
Bso31I GGTCTC 1 cut(s) 318
Bsp119I TTCGAA 1 cut(s) 60
Bsp1407I TGTACA 1 cut(s) 502
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 3 cut(s) 26, 66, 88
BspANI GGCC 4 cut(s) 7, 141, 159, 196
BspCNI CTCAG 1 cut(s) 13
BspFNI CGCG 2 cut(s) 90, 150
BspLI GGNNCC 4 cut(s) 121, 230, 418, 603
BspMI ACCTGC 1 cut(s) 589
BspT104I TTCGAA 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 318
BsrDI GCAATG 1 cut(s) 456
BsrGI TGTACA 1 cut(s) 502
BsrI ACTGG 3 cut(s) 230, 429, 714
BssECI CCNNGG 3 cut(s) 115, 387, 518
BssNAI GTATAC 1 cut(s) 73
BssNI GRCGYC 2 cut(s) 47, 78
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 2 cut(s) 387, 518
Bst1107I GTATAC 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 2 cut(s) 143, 374
BstACI GRCGYC 2 cut(s) 47, 78
BstAUI TGTACA 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 862, 866
BstDEI CTNAG 3 cut(s) 21, 358, 470
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 2 cut(s) 418, 514
BstFNI CGCG 2 cut(s) 90, 150
BstHHI GCGC 2 cut(s) 92, 152
BstMAI GTCTC 1 cut(s) 318
BstMWI GCNNNNNNNGC 6 cut(s) 75, 84, 147, 156, 226, 716
BstNI CCWGG 2 cut(s) 143, 374
BstNSI RCATGY 2 cut(s) 656, 864
BstSCI CCNGG 4 cut(s) 115, 141, 231, 372
BstUI CGCG 2 cut(s) 90, 150
BstV1I GCAGC 2 cut(s) 697, 749
BstV2I GAAGAC 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 427
BstZ17I GTATAC 1 cut(s) 73
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 4 cut(s) 7, 141, 159, 196
BtgI CCRYGG 1 cut(s) 387
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 2 cut(s) 418, 514
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 4 cut(s) 223, 343, 721, 801
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 2 cut(s) 862, 866
CfoI GCGC 2 cut(s) 92, 152
Cfr13I GGNCC 3 cut(s) 416, 658, 776
CseI GACGC 2 cut(s) 55, 67
Csp6I GTAC 2 cut(s) 83, 503
CviAII CATG 4 cut(s) 388, 653, 720, 861
CviQI GTAC 2 cut(s) 83, 503
DdeI CTNAG 3 cut(s) 21, 358, 470
DraIII CACNNNGTG 1 cut(s) 178
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 387, 518
Eco31I GGTCTC 1 cut(s) 318
Eco47I GGWCC 3 cut(s) 416, 658, 776
Eco57I CTGAAG 1 cut(s) 606
EcoRII CCWGG 2 cut(s) 141, 372
EcoT14I CCWWGG 2 cut(s) 387, 518
ErhI CCWWGG 2 cut(s) 387, 518
FaeI CATG 4 cut(s) 391, 656, 723, 864
FaqI GGGAC 1 cut(s) 58
FatI CATG 4 cut(s) 387, 652, 719, 860
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 5 cut(s) 26, 67, 88, 711, 738
FokI GGATG 2 cut(s) 425, 501
Fsp4HI GCNGC 5 cut(s) 26, 67, 88, 711, 738
FspBI CTAG 3 cut(s) 479, 519, 869
GlaI GCGC 2 cut(s) 91, 151
GluI GCNGC 5 cut(s) 26, 67, 88, 711, 738
GsaI CCCAGC 2 cut(s) 33, 345
HaeIII GGCC 4 cut(s) 7, 141, 159, 196
HapII CCGG 3 cut(s) 50, 117, 233
HgaI GACGC 2 cut(s) 55, 67
HhaI GCGC 2 cut(s) 92, 152
Hin1I GRCGYC 2 cut(s) 47, 78
Hin1II CATG 4 cut(s) 391, 656, 723, 864
Hin6I GCGC 2 cut(s) 90, 150
HinP1I GCGC 2 cut(s) 90, 150
HinfI GANTC 2 cut(s) 127, 806
HpaII CCGG 3 cut(s) 50, 117, 233
Hpy166II GTNNAC 3 cut(s) 73, 244, 638
Hpy188I TCNGA 4 cut(s) 124, 132, 399, 487
Hpy188III TCNNGA 4 cut(s) 20, 97, 635, 644
Hpy8I GTNNAC 3 cut(s) 73, 244, 638
Hpy99I CGWCG 3 cut(s) 83, 98, 179
HpyAV CCTTC 2 cut(s) 56, 710
HpyCH4IV ACGT 4 cut(s) 13, 174, 492, 616
HpyCH4V TGCA 5 cut(s) 461, 526, 679, 719, 737
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 84, 147, 156, 226, 716
HpyF3I CTNAG 3 cut(s) 21, 358, 470
HpySE526I ACGT 4 cut(s) 13, 174, 492, 616
Hsp92I GRCGYC 2 cut(s) 47, 78
Hsp92II CATG 4 cut(s) 391, 656, 723, 864
HspAI GCGC 2 cut(s) 90, 150
LmnI GCTCC 2 cut(s) 234, 671
Lsp1109I GCAGC 2 cut(s) 697, 749
LweI GCATC 5 cut(s) 491, 535, 679, 688, 846
MaeI CTAG 3 cut(s) 479, 519, 869
MaeII ACGT 4 cut(s) 13, 174, 492, 616
MaeIII GTNAC 4 cut(s) 187, 382, 550, 820
MboII GAAGA 3 cut(s) 8, 49, 283
MluCI AATT 4 cut(s) 608, 684, 731, 892
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 7 cut(s) 170, 194, 197, 615, 642, 651, 671
MseI TTAA 2 cut(s) 287, 895
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 3 cut(s) 50, 117, 233
MspR9I CCNGG 4 cut(s) 117, 143, 233, 374
MvaI CCWGG 2 cut(s) 143, 374
MvnI CGCG 2 cut(s) 90, 150
MwoI GCNNNNNNNGC 6 cut(s) 75, 84, 147, 156, 226, 716
NciI CCSGG 2 cut(s) 117, 233
NcoI CCATGG 1 cut(s) 387
NlaIII CATG 4 cut(s) 391, 656, 723, 864
NlaIV GGNNCC 4 cut(s) 121, 230, 418, 603
NmeAIII GCCGAG 1 cut(s) 135
NspI RCATGY 2 cut(s) 656, 864
NspV TTCGAA 1 cut(s) 60
PaeI GCATGC 1 cut(s) 864
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 162
PfeI GAWTC 2 cut(s) 127, 806
Pfl23II CGTACG 1 cut(s) 82
PflMI CCANNNNNTGG 1 cut(s) 720
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 5 cut(s) 27, 68, 89, 712, 739
Ppu21I YACGTR 1 cut(s) 617
Psp6I CCWGG 2 cut(s) 141, 372
PspFI CCCAGC 2 cut(s) 29, 341
PspGI CCWGG 2 cut(s) 141, 372
PspLI CGTACG 1 cut(s) 82
PspN4I GGNNCC 4 cut(s) 121, 230, 418, 603
PspPI GGNCC 3 cut(s) 416, 658, 776
RsaI GTAC 2 cut(s) 84, 504
RsaNI GTAC 2 cut(s) 83, 503
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 2 cut(s) 287, 895
SatI GCNGC 5 cut(s) 26, 67, 88, 711, 738
Sau96I GGNCC 3 cut(s) 416, 658, 776
ScrFI CCNGG 4 cut(s) 117, 143, 233, 374
SfaNI GCATC 5 cut(s) 491, 535, 679, 688, 846
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 3 cut(s) 416, 658, 776
SmiMI CAYNNNNRTG 1 cut(s) 425
SphI GCATGC 1 cut(s) 864
Sse9I AATT 4 cut(s) 608, 684, 731, 892
SsiI CCGC 3 cut(s) 26, 66, 88
SspMI CTAG 3 cut(s) 479, 519, 869
StyD4I CCNGG 4 cut(s) 115, 141, 231, 372
StyI CCWWGG 2 cut(s) 387, 518
TaiI ACGT 4 cut(s) 16, 177, 495, 619
TaqI TCGA 2 cut(s) 60, 184
TasI AATT 4 cut(s) 608, 684, 731, 892
TatI WGTACW 1 cut(s) 502
TauI GCSGC 3 cut(s) 28, 69, 90
TfiI GAWTC 2 cut(s) 127, 806
Tru1I TTAA 2 cut(s) 287, 895
Tru9I TTAA 2 cut(s) 287, 895
TscAI CASTG 4 cut(s) 230, 343, 721, 808
TseI GCWGC 2 cut(s) 710, 737
TspDTI ATGAA 2 cut(s) 420, 744
TspRI CASTG 4 cut(s) 230, 343, 721, 808
Van91I CCANNNNNTGG 1 cut(s) 720
VpaK11BI GGWCC 3 cut(s) 416, 658, 776
XapI RAATTY 1 cut(s) 731
XceI RCATGY 2 cut(s) 656, 864
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmaJI CCTAGG 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 3 cut(s) 479, 519, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.