FvH4_3g29040

E3 ubiquitin ligase BIG BROTHER-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010546
Physical Location & Seq
Reverse (-)
22163074 .. 22171070
7997 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29040.t1

Sequence Viewer

Length: 759 bp
ATGAGTTGGAATCCGCATATGGGAGTTCATTACCCCTACAACAGCTGTCCCTATAACTCGGCTGGTAGCTTTATGGACTATTTTGAAGGGCTCACCTATGAACATGTGAACTTTATTTTTTCAGGGGATTCACATGCTCAGGAGAGTACTTACCCATCAATGAACACGAACTATTACAAGTTTGGGTTAACTGAACCTGGGAATACTTCATATTATGATCTTGGTTTTCGTCATGCCTACGAGTTCAATGATCCTGTCGGACGAAGTGGTGAAGAAACAAGGCTTTTCCAGCACTCTTCGACTATTCTTAATGAACAGACTGTGGCAAGGGATCCACTATGTGAAGGAAGTGCAAACACTTCTACATGTGACAACCCCAGAGAATCTCCTCGGAGATATCAGAACTCCCATGACTATCAGGTTATTTTGGAAGACAACATTGACCCTGATAGCATGACTTATGAGGAATTACTAGAATTAGGTGAGGCAGTTGGAACTGAAAGCCGAGGTCTTTCCCAAGATCTGCTTTCTTTGCTTCCAATCTCGAAATACAAGTGCAGCTTTTTCTCAAGAAAGAAATCACGGAGTGAGAGGTGTGTGATCTGCCAGATGGAATATAAACGAGGGGACCGGCAAATTACTCTTCCATGCAAACACCTCTATCATGCTGGATGTGGAACCAGATGGCTTAGTATGAATAAGGCTTGCCCAATATGCTACACTGAGGTATTTGGTAAAGGATCGATGAGCGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

253

Amino Acids

28.86

Weight (kDa)

5.6

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 194 - 239 1.3e-06 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 196 - 243 7.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 198 - 239 1e-12 Ring finger domain
zf-C3HC4_2 PF13923 198 - 239 1.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 199 - 239 1.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 199 - 225 1.2e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 14
AclWI GGATC 4 cut(s) 245, 326, 339, 748
AdeI CACNNNGTG 2 cut(s) 341, 587
AfaI GTAC 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 20
AflIII ACRYGT 2 cut(s) 103, 365
AgsI TTSAA 2 cut(s) 86, 247
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 3 cut(s) 45, 69, 561
AluI AGCT 3 cut(s) 45, 69, 561
AlwI GGATC 4 cut(s) 245, 326, 339, 748
ApeKI GCWGC 1 cut(s) 558
AspS9I GGNCC 1 cut(s) 628
AsuHPI GGTGA 3 cut(s) 85, 281, 494
AvaII GGWCC 1 cut(s) 628
BamHI GGATCC 1 cut(s) 331
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 570
BccI CCATC 3 cut(s) 163, 604, 678
BciT130I CCWGG 1 cut(s) 198
BfaI CTAG 1 cut(s) 473
BglII AGATCT 1 cut(s) 520
BisI GCNGC 1 cut(s) 559
BlsI GCNGC 1 cut(s) 560
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 198
Bme18I GGWCC 1 cut(s) 628
BmgT120I GGNCC 1 cut(s) 628
BmiI GGNNCC 3 cut(s) 333, 629, 679
BmrFI CCNGG 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 438
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 553
Bsa29I ATCGAT 1 cut(s) 743
BsaJI CCNNGG 3 cut(s) 197, 389, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 20
Bse118I RCCGGY 1 cut(s) 630
BseBI CCWGG 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 743
BseDI CCNNGG 3 cut(s) 197, 389, 505
BseGI GGATG 1 cut(s) 677
BseLI CCNNNNNNNGG 1 cut(s) 20
BseMII CTCAG 2 cut(s) 152, 714
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 1 cut(s) 570
BsgI GTGCAG 1 cut(s) 577
BshVI ATCGAT 1 cut(s) 743
BsiSI CCGG 1 cut(s) 631
BslFI GGGAC 2 cut(s) 33, 641
BslI CCNNNNNNNGG 1 cut(s) 20
BsmFI GGGAC 2 cut(s) 33, 641
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 6 cut(s) 217, 250, 331, 520, 600, 740
BspACI CCGC 1 cut(s) 14
BspCNI CTCAG 2 cut(s) 151, 715
BspDI ATCGAT 1 cut(s) 743
BspLI GGNNCC 3 cut(s) 333, 629, 679
BspPI GGATC 4 cut(s) 245, 326, 339, 748
BsrFI RCCGGY 1 cut(s) 630
BssAI RCCGGY 1 cut(s) 630
BssECI CCNNGG 3 cut(s) 197, 389, 505
BssMI GATC 6 cut(s) 217, 250, 331, 520, 600, 740
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 2 cut(s) 301, 648
BstC8I GCNNGC 1 cut(s) 706
BstDEI CTNAG 3 cut(s) 138, 689, 723
BstF5I GGATG 1 cut(s) 677
BstKTI GATC 6 cut(s) 220, 253, 334, 523, 603, 743
BstMBI GATC 6 cut(s) 217, 250, 331, 520, 600, 740
BstMWI GCNNNNNNNGC 3 cut(s) 289, 532, 714
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 3 cut(s) 107, 137, 369
BstSCI CCNGG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 570
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 331, 520
BstYI RGATCY 2 cut(s) 331, 520
Bsu15I ATCGAT 1 cut(s) 743
BsuTUI ATCGAT 1 cut(s) 743
BtsCI GGATG 1 cut(s) 677
BtsIMutI CAGTG 1 cut(s) 720
Cac8I GCNNGC 1 cut(s) 706
Cfr10I RCCGGY 1 cut(s) 630
Cfr13I GGNCC 1 cut(s) 628
ClaI ATCGAT 1 cut(s) 743
Csp6I GTAC 1 cut(s) 147
CviAII CATG 8 cut(s) 104, 134, 233, 366, 410, 454, 648, 665
CviJI RGCY 9 cut(s) 45, 62, 69, 91, 283, 504, 561, 688, 704
CviKI_1 RGCY 9 cut(s) 45, 62, 69, 91, 283, 504, 561, 688, 704
CviQI GTAC 1 cut(s) 147
DdeI CTNAG 3 cut(s) 138, 689, 723
DpnI GATC 6 cut(s) 219, 252, 333, 522, 602, 742
DpnII GATC 6 cut(s) 217, 250, 331, 520, 600, 740
DraIII CACNNNGTG 2 cut(s) 341, 587
Eam1104I CTCTTC 2 cut(s) 301, 648
EarI CTCTTC 2 cut(s) 301, 648
Eco24I GRGCYC 1 cut(s) 93
Eco32I GATATC 1 cut(s) 398
Eco47I GGWCC 1 cut(s) 628
EcoRII CCWGG 1 cut(s) 196
EcoRV GATATC 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 8 cut(s) 107, 137, 236, 369, 413, 457, 651, 668
FalI AAGNNNNNCTT 4 cut(s) 510, 542, 545, 577
FaqI GGGAC 2 cut(s) 33, 641
FatI CATG 8 cut(s) 103, 133, 232, 365, 409, 453, 647, 664
FauNDI CATATG 1 cut(s) 18
Fnu4HI GCNGC 1 cut(s) 559
FokI GGATG 1 cut(s) 684
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 559
FspBI CTAG 1 cut(s) 473
GluI GCNGC 1 cut(s) 559
HapII CCGG 1 cut(s) 631
Hin1II CATG 8 cut(s) 107, 137, 236, 369, 413, 457, 651, 668
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 3 cut(s) 10, 128, 383
HpaI GTTAAC 1 cut(s) 189
HpaII CCGG 1 cut(s) 631
HphI GGTGA 3 cut(s) 85, 281, 494
Hpy166II GTNNAC 2 cut(s) 109, 189
Hpy188I TCNGA 3 cut(s) 260, 393, 402
Hpy188III TCNNGA 3 cut(s) 140, 544, 570
Hpy8I GTNNAC 2 cut(s) 109, 189
HpyAV CCTTC 2 cut(s) 80, 338
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4V TGCA 3 cut(s) 353, 558, 651
HpyF10VI GCNNNNNNNGC 3 cut(s) 289, 532, 714
HpyF3I CTNAG 3 cut(s) 138, 689, 723
Hsp92II CATG 8 cut(s) 107, 137, 236, 369, 413, 457, 651, 668
KspAI GTTAAC 1 cut(s) 189
Kzo9I GATC 6 cut(s) 217, 250, 331, 520, 600, 740
Lsp1109I GCAGC 1 cut(s) 570
MaeI CTAG 1 cut(s) 473
MaeIII GTNAC 1 cut(s) 368
MalI GATC 6 cut(s) 219, 252, 333, 522, 602, 742
MboI GATC 6 cut(s) 217, 250, 331, 520, 600, 740
MboII GAAGA 4 cut(s) 284, 288, 443, 635
MflI RGATCY 2 cut(s) 331, 520
MhlI GDGCHC 1 cut(s) 93
MluCI AATT 3 cut(s) 467, 476, 636
MmeI TCCRAC 2 cut(s) 238, 472
MnlI CCTC 8 cut(s) 399, 457, 478, 500, 585, 617, 668, 718
MseI TTAA 2 cut(s) 188, 309
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 1 cut(s) 631
MspR9I CCNGG 1 cut(s) 198
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 3 cut(s) 289, 532, 714
NdeI CATATG 1 cut(s) 18
NdeII GATC 6 cut(s) 217, 250, 331, 520, 600, 740
NlaIII CATG 8 cut(s) 107, 137, 236, 369, 413, 457, 651, 668
NlaIV GGNNCC 3 cut(s) 333, 629, 679
NmeAIII GCCGAG 2 cut(s) 38, 530
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 3 cut(s) 107, 137, 369
PciI ACATGT 2 cut(s) 103, 365
PfeI GAWTC 3 cut(s) 10, 128, 383
PkrI GCNGC 1 cut(s) 560
PscI ACATGT 2 cut(s) 103, 365
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 3 cut(s) 333, 629, 679
PspPI GGNCC 1 cut(s) 628
PsuI RGATCY 2 cut(s) 331, 520
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
SaqAI TTAA 2 cut(s) 188, 309
SatI GCNGC 1 cut(s) 559
Sau3AI GATC 6 cut(s) 217, 250, 331, 520, 600, 740
Sau96I GGNCC 1 cut(s) 628
ScaI AGTACT 1 cut(s) 148
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 1 cut(s) 93
SinI GGWCC 1 cut(s) 628
SmlI CTYRAG 1 cut(s) 568
SmoI CTYRAG 1 cut(s) 568
Sse9I AATT 3 cut(s) 467, 476, 636
SsiI CCGC 1 cut(s) 14
SspMI CTAG 1 cut(s) 473
StyD4I CCNGG 1 cut(s) 196
TaaI ACNGT 1 cut(s) 322
TaqI TCGA 3 cut(s) 299, 545, 743
TasI AATT 3 cut(s) 467, 476, 636
TatI WGTACW 1 cut(s) 146
TfiI GAWTC 3 cut(s) 10, 128, 383
Tru1I TTAA 2 cut(s) 188, 309
Tru9I TTAA 2 cut(s) 188, 309
TscAI CASTG 1 cut(s) 727
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 1 cut(s) 558
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 6 cut(s) 17, 114, 176, 198, 327, 710
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 1 cut(s) 727
VpaK11BI GGWCC 1 cut(s) 628
XceI RCATGY 3 cut(s) 107, 137, 369
XspI CTAG 1 cut(s) 473
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.