Rw5G033120

E3 ubiquitin ligase BIG BROTHER-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010546
Physical Location & Seq
Reverse (-)
56171129 .. 56176775
5647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033120.1

Sequence Viewer

Length: 759 bp
ATGAGTTGGAACCCGCATATGGGAGTTCATTACCCCTACAACAGCTGTCCCTATAGCACGGTGGGTAGCTTTATGGACTATGTTGAAGGCCTCACCTACGAACATGTGAACTTTATTTTTTCAGGGGATTCACATGCTCAGGAGAGCACTTACCCTTCAATGAATACGAACTATTACAAGTTTGGGTCAACTGAACCTGGGAATACTTCATATTATGAACTTGGATTTCGTCATTCCTATGAGTTCAATGATCCTATCTCAAGAAGTGGTGAAGAAACAAGGCCTTTGCAGTACTCTTCAACTATTCCTAATGAACAGAATGTGGCAAGGGATCCACCATGCGAAGGAAGTGCAAACACTTCCACATGTGACAACCCCAGAGAATCCCCTCGAAGATATCAGAACTCCCAGGATTATCAGGTTATTTTGGAAGACAACATTGACCCTGATAGCATGACTTACGAGGAATTACTAGAATTAGGTGAGGCAGTTGGAACTGAAAGCCGAGGTCTTACCCAAGATATGCTTTCTTTGCTTCCAATCTCAAAGTACAAGTGCAGCTTTTTCTCAAGAAAGAAATCACGGAGTGAGAGGTGTGTGATTTGCCAGATGGAATATAAACGAGGGGATCGGCAGATCACTCTGCCGTGCAAACACCTCTATCATGCTGGATGTGGAACCAGATGGCTTAGCATGAACAAGGCTTGCCCAGTATGCTACACCGAGGTATTTGGTAATGGATCGATGAGCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

252

Amino Acids

28.83

Weight (kDa)

5.53

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 194 - 239 1.6e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 198 - 239 9.5e-13 Ring finger domain
zf-C3HC4_2 PF13923 198 - 239 1.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 199 - 239 5.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 199 - 225 1.2e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 14
AclWI GGATC 5 cut(s) 245, 326, 339, 636, 748
AdeI CACNNNGTG 1 cut(s) 587
AfaI GTAC 2 cut(s) 293, 551
AfiI CCNNNNNNNGG 3 cut(s) 19, 20, 344
AflIII ACRYGT 2 cut(s) 103, 365
AgsI TTSAA 4 cut(s) 86, 159, 247, 300
AjnI CCWGG 2 cut(s) 196, 408
AluBI AGCT 3 cut(s) 45, 69, 561
AluI AGCT 3 cut(s) 45, 69, 561
Alw21I GWGCWC 1 cut(s) 149
AlwI GGATC 5 cut(s) 245, 326, 339, 636, 748
AoxI GGCC 2 cut(s) 88, 281
ApeKI GCWGC 1 cut(s) 558
AsuHPI GGTGA 3 cut(s) 85, 281, 494
BamHI GGATCC 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 570
BccI CCATC 2 cut(s) 604, 678
BceAI ACGGC 1 cut(s) 631
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 2 cut(s) 198, 410
BfaI CTAG 1 cut(s) 473
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 1 cut(s) 559
BlpI GCTNAGC 1 cut(s) 689
BlsI GCNGC 1 cut(s) 560
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 2 cut(s) 198, 410
BmiI GGNNCC 3 cut(s) 11, 333, 679
BmrFI CCNGG 2 cut(s) 198, 410
BmrI ACTGGG 1 cut(s) 704
BmuI ACTGGG 1 cut(s) 704
BpiI GAAGAC 1 cut(s) 438
Bpu10I CCTNAGC 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 689
BpuEI CTTGAG 2 cut(s) 244, 553
Bsa29I ATCGAT 1 cut(s) 743
BsaJI CCNNGG 4 cut(s) 197, 408, 505, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 20, 344
Bse1I ACTGG 1 cut(s) 710
BseBI CCWGG 2 cut(s) 198, 410
BseCI ATCGAT 1 cut(s) 743
BseDI CCNNGG 4 cut(s) 197, 408, 505, 723
BseGI GGATG 1 cut(s) 677
BseLI CCNNNNNNNGG 3 cut(s) 19, 20, 344
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 710
BseXI GCAGC 1 cut(s) 570
BsgI GTGCAG 1 cut(s) 577
BshFI GGCC 2 cut(s) 90, 283
BshVI ATCGAT 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 149
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 3 cut(s) 19, 20, 344
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 2 cut(s) 90, 283
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 5 cut(s) 250, 331, 628, 636, 740
Bsp1720I GCTNAGC 1 cut(s) 689
BspACI CCGC 1 cut(s) 14
BspANI GGCC 2 cut(s) 90, 283
BspCNI CTCAG 1 cut(s) 151
BspDI ATCGAT 1 cut(s) 743
BspLI GGNNCC 3 cut(s) 11, 333, 679
BspPI GGATC 5 cut(s) 245, 326, 339, 636, 748
BsrI ACTGG 1 cut(s) 710
BssECI CCNNGG 4 cut(s) 197, 408, 505, 723
BssMI GATC 5 cut(s) 250, 331, 628, 636, 740
Bst2UI CCWGG 2 cut(s) 198, 410
Bst4CI ACNGT 1 cut(s) 61
Bst6I CTCTTC 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 706
BstDEI CTNAG 2 cut(s) 138, 689
BstF5I GGATG 1 cut(s) 677
BstKTI GATC 5 cut(s) 253, 334, 631, 639, 743
BstMBI GATC 5 cut(s) 250, 331, 628, 636, 740
BstMWI GCNNNNNNNGC 2 cut(s) 532, 714
BstNI CCWGG 2 cut(s) 198, 410
BstNSI RCATGY 3 cut(s) 107, 137, 369
BstSCI CCNGG 2 cut(s) 196, 408
BstSFI CTRYAG 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 570
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
Bsu15I ATCGAT 1 cut(s) 743
BsuRI GGCC 2 cut(s) 90, 283
BsuTUI ATCGAT 1 cut(s) 743
BtsCI GGATG 1 cut(s) 677
Cac8I GCNNGC 1 cut(s) 706
ClaI ATCGAT 1 cut(s) 743
Csp6I GTAC 2 cut(s) 292, 550
CviAII CATG 7 cut(s) 104, 134, 339, 366, 454, 665, 694
CviJI RGCY 8 cut(s) 45, 69, 90, 283, 504, 561, 688, 704
CviKI_1 RGCY 8 cut(s) 45, 69, 90, 283, 504, 561, 688, 704
CviQI GTAC 2 cut(s) 292, 550
DdeI CTNAG 2 cut(s) 138, 689
DpnI GATC 5 cut(s) 252, 333, 630, 638, 742
DpnII GATC 5 cut(s) 250, 331, 628, 636, 740
DraIII CACNNNGTG 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 301
EarI CTCTTC 1 cut(s) 301
Eco147I AGGCCT 2 cut(s) 90, 283
Eco32I GATATC 1 cut(s) 398
EcoRII CCWGG 2 cut(s) 196, 408
EcoRV GATATC 1 cut(s) 398
FaeI CATG 7 cut(s) 107, 137, 342, 369, 457, 668, 697
FalI AAGNNNNNCTT 4 cut(s) 510, 542, 545, 577
FaqI GGGAC 1 cut(s) 33
FatI CATG 7 cut(s) 103, 133, 338, 365, 453, 664, 693
FauI CCCGC 1 cut(s) 21
FauNDI CATATG 1 cut(s) 18
Fnu4HI GCNGC 1 cut(s) 559
FokI GGATG 1 cut(s) 684
Fsp4HI GCNGC 1 cut(s) 559
FspBI CTAG 1 cut(s) 473
GluI GCNGC 1 cut(s) 559
HaeIII GGCC 2 cut(s) 90, 283
Hin1II CATG 7 cut(s) 107, 137, 342, 369, 457, 668, 697
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 2 cut(s) 128, 383
HphI GGTGA 3 cut(s) 85, 281, 494
Hpy166II GTNNAC 2 cut(s) 109, 189
Hpy188I TCNGA 1 cut(s) 402
Hpy188III TCNNGA 3 cut(s) 140, 261, 570
Hpy8I GTNNAC 2 cut(s) 109, 189
HpyAV CCTTC 3 cut(s) 80, 165, 338
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4V TGCA 4 cut(s) 289, 353, 558, 651
HpyF10VI GCNNNNNNNGC 2 cut(s) 532, 714
HpyF3I CTNAG 2 cut(s) 138, 689
Hsp92II CATG 7 cut(s) 107, 137, 342, 369, 457, 668, 697
Kzo9I GATC 5 cut(s) 250, 331, 628, 636, 740
Lsp1109I GCAGC 1 cut(s) 570
MaeI CTAG 1 cut(s) 473
MaeIII GTNAC 1 cut(s) 368
MalI GATC 5 cut(s) 252, 333, 630, 638, 742
MboI GATC 5 cut(s) 250, 331, 628, 636, 740
MboII GAAGA 4 cut(s) 284, 288, 405, 443
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 2 cut(s) 467, 476
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 9 cut(s) 101, 399, 457, 478, 500, 585, 617, 668, 718
MslI CAYNNNNRTG 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 45
MspR9I CCNGG 2 cut(s) 198, 410
MvaI CCWGG 2 cut(s) 198, 410
MwoI GCNNNNNNNGC 2 cut(s) 532, 714
NdeI CATATG 1 cut(s) 18
NdeII GATC 5 cut(s) 250, 331, 628, 636, 740
NlaIII CATG 7 cut(s) 107, 137, 342, 369, 457, 668, 697
NlaIV GGNNCC 3 cut(s) 11, 333, 679
NmeAIII GCCGAG 1 cut(s) 530
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 3 cut(s) 107, 137, 369
PceI AGGCCT 2 cut(s) 90, 283
PciI ACATGT 2 cut(s) 103, 365
PfeI GAWTC 2 cut(s) 128, 383
PkrI GCNGC 1 cut(s) 560
PscI ACATGT 2 cut(s) 103, 365
Psp6I CCWGG 2 cut(s) 196, 408
PspGI CCWGG 2 cut(s) 196, 408
PspN4I GGNNCC 3 cut(s) 11, 333, 679
PsuI RGATCY 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 2 cut(s) 293, 551
RsaNI GTAC 2 cut(s) 292, 550
RseI CAYNNNNRTG 1 cut(s) 237
SatI GCNGC 1 cut(s) 559
Sau3AI GATC 5 cut(s) 250, 331, 628, 636, 740
ScaI AGTACT 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 198, 410
SduI GDGCHC 1 cut(s) 149
SfcI CTRYAG 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 2 cut(s) 259, 568
SmoI CTYRAG 2 cut(s) 259, 568
Sse9I AATT 2 cut(s) 467, 476
SseBI AGGCCT 2 cut(s) 90, 283
SsiI CCGC 1 cut(s) 14
SspMI CTAG 1 cut(s) 473
StuI AGGCCT 2 cut(s) 90, 283
StyD4I CCNGG 2 cut(s) 196, 408
TaaI ACNGT 1 cut(s) 61
TaqI TCGA 2 cut(s) 391, 743
TasI AATT 2 cut(s) 467, 476
TatI WGTACW 2 cut(s) 291, 549
TfiI GAWTC 2 cut(s) 128, 383
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 1 cut(s) 558
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 6 cut(s) 17, 176, 198, 231, 327, 710
TspGWI ACGGA 1 cut(s) 598
XceI RCATGY 3 cut(s) 107, 137, 369
XspI CTAG 1 cut(s) 473
ZrmI AGTACT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.