Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010546

Physical Location & Seq

Genomic Coordinates Reverse (-)

24511872 .. 24516676

Gene Structure 4805 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1178200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 738 bp

ATGAGTTGGAACCCACACATGGAGGTTCATTACACCTACACCAACTGTCCTTATAGCTCAGCGGGTAGCTTTATGGAGTATTTTGAGGGTCTTACCTATGAACATGTGAACTTTATTTTTTCAGGCGATTCGCATGCTCAGGAGAGTGCTTACCCAACAATGAATACAAATTATTACAAGTTTGGGTTGTCAGAACCTGGGAATATTACTTCATGCTATGATCTTGGGTTTGGTCATGCTTATGAGATCAATGGTCCTGACCCAAGAACTGGGGAACAAAGAAGGCATTTGCAGAGCGCTTCAACAATGACTAATGAACACAATGTGGCTGTGAACTCAGAATGGGAAGGAAATGTGAATACTTCTACACGCCACAATCCCAGAGAATGCCCACGGAGACATCAAAATTCCGATGATTACCAGGTTATTTGGCAAGACAACATTGATCCTGACATCATGAGTTACGAGGAATTACTTGAGTTAGGTGAGACAGTTGGCACTCAAAATCGAGGTCTCTCCCAAGATCAGATCTCCTTGCTTCCAATCTCGAAGTACAAATGCAGCTTTTTCTCGAGAAAGAAATCACGAGACGAGAGATGTGTGATTTGCCAGATGGAATATAAACGAGGGGACCGGCGGATTGCTCTACCGTGCAAACACCTCTATCATGCTGGTTGTGGGACCAGATGGCTTAGCATCAACAAGGCAAGCTGTTGCTTGCCCAATATGTTACACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

246

Amino Acids

28.28

Weight (kDa)

6.04

Isoelectric Point (pI)

49.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	199 - 238	7.2e-09	Ring finger domain
zf-RING_11	PF17123	200 - 226	3.4e-07	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010546)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29040
malus_domestica	MD03G1178200.v1.1 MD11G1198000.v1.1
prunus_persica	Prupe.4G244600_v2.0.a1 Prupe.4G244600_v2.0.a1 Prupe.4G244600_v2.0.a1 Prupe.4G244600_v2.0.a1
pyrus_communis	pycom03g13490 pycom11g17140
rosa_chinensis	RchiOBHm_Chr5g0051621
rosa_laevigata	RLG00000034872
rosa_multiflora	Rmu_ssc0000089.1_g000034
rosa_roxburghii	Rroxscaffold_1G00027100
rosa_rugosa	Rorug05G0278700 Rorug05G0278800 Rorug05G0278900
rosa_samantha	Rh5AG350000 Rh5BG361800 Rh5CG386700 Rh5DG376700
rosa_wichuraiana	Rw5G033120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	269
AciI	CCGC	2 cut(s)	62, 637
AclWI	GGATC	1 cut(s)	440
AcsI	RAATTY	1 cut(s)	406
AdeI	CACNNNGTG	1 cut(s)	325
AfaI	GTAC	1 cut(s)	554
AfeI	AGCGCT	1 cut(s)	298
AfiI	CCNNNNNNNGG	2 cut(s)	19, 269
AflIII	ACRYGT	1 cut(s)	103
AgsI	TTSAA	1 cut(s)	303
AjnI	CCWGG	2 cut(s)	196, 420
AluBI	AGCT	4 cut(s)	57, 69, 564, 711
AluI	AGCT	4 cut(s)	57, 69, 564, 711
Alw26I	GTCTC	4 cut(s)	391, 482, 518, 582
AlwI	GGATC	1 cut(s)	440
AlwNI	CAGNNNCTG	1 cut(s)	197
Ama87I	CYCGRG	1 cut(s)	571
Aor51HI	AGCGCT	1 cut(s)	298
ApeKI	GCWGC	1 cut(s)	561
ApoI	RAATTY	1 cut(s)	406
ArsI	GACNNNNNNTTYG	2 cut(s)	496, 528
AspLEI	GCGC	1 cut(s)	299
AspS9I	GGNCC	3 cut(s)	254, 631, 681
AsuHPI	GGTGA	1 cut(s)	497
AvaI	CYCGRG	1 cut(s)	571
AvaII	GGWCC	3 cut(s)	254, 631, 681
BauI	CACGAG	1 cut(s)	585
BbvI	GCAGC	1 cut(s)	573
BccI	CCATC	2 cut(s)	607, 681
BcgI	CGANNNNNNTGC	2 cut(s)	116, 150
BciT130I	CCWGG	2 cut(s)	198, 422
BcoDI	GTCTC	4 cut(s)	391, 482, 518, 582
BfoI	RGCGCY	1 cut(s)	300
BglII	AGATCT	1 cut(s)	528
BisI	GCNGC	1 cut(s)	562
BlpI	GCTNAGC	2 cut(s)	58, 692
BlsI	GCNGC	1 cut(s)	563
Bme1390I	CCNGG	2 cut(s)	198, 422
Bme18I	GGWCC	3 cut(s)	254, 631, 681
BmeT110I	CYCGRG	1 cut(s)	571
BmgT120I	GGNCC	3 cut(s)	254, 631, 681
BmiI	GGNNCC	3 cut(s)	11, 632, 682
BmrFI	CCNGG	2 cut(s)	198, 422
BmrI	ACTGGG	1 cut(s)	279
BmsI	GCATC	1 cut(s)	705
BmuI	ACTGGG	1 cut(s)	279
Bpu10I	CCTNAGC	1 cut(s)	138
Bpu1102I	GCTNAGC	2 cut(s)	58, 692
BpuEI	CTTGAG	1 cut(s)	497
BsaI	GGTCTC	1 cut(s)	518
BsaJI	CCNNGG	2 cut(s)	197, 392
Bsc4I	CCNNNNNNNGG	2 cut(s)	19, 269
Bse118I	RCCGGY	1 cut(s)	633
Bse1I	ACTGG	1 cut(s)	274
BseBI	CCWGG	2 cut(s)	198, 422
BseDI	CCNNGG	2 cut(s)	197, 392
BseLI	CCNNNNNNNGG	2 cut(s)	19, 269
BseMII	CTCAG	3 cut(s)	72, 152, 351
BseNI	ACTGG	1 cut(s)	274
BseXI	GCAGC	1 cut(s)	573
BsiHKCI	CYCGRG	1 cut(s)	571
BsiSI	CCGG	1 cut(s)	634
BslFI	GGGAC	2 cut(s)	644, 694
BslI	CCNNNNNNNGG	2 cut(s)	19, 269
BsmAI	GTCTC	4 cut(s)	391, 482, 518, 582
BsmBI	CGTCTC	1 cut(s)	582
BsmFI	GGGAC	2 cut(s)	644, 694
BsmI	GAATGC	1 cut(s)	392
Bso31I	GGTCTC	1 cut(s)	518
BsoBI	CYCGRG	1 cut(s)	571
Bsp143I	GATC	5 cut(s)	220, 246, 445, 523, 528
Bsp1720I	GCTNAGC	2 cut(s)	58, 692
BspACI	CCGC	2 cut(s)	62, 637
BspCNI	CTCAG	3 cut(s)	71, 151, 350
BspHI	TCATGA	1 cut(s)	456
BspLI	GGNNCC	3 cut(s)	11, 632, 682
BspPI	GGATC	1 cut(s)	440
BspTNI	GGTCTC	1 cut(s)	518
BsrFI	RCCGGY	1 cut(s)	633
BsrI	ACTGG	1 cut(s)	274
BssAI	RCCGGY	1 cut(s)	633
BssECI	CCNNGG	2 cut(s)	197, 392
BssMI	GATC	5 cut(s)	220, 246, 445, 523, 528
BssSI	CACGAG	1 cut(s)	585
Bst2BI	CACGAG	1 cut(s)	585
Bst2UI	CCWGG	2 cut(s)	198, 422
Bst4CI	ACNGT	3 cut(s)	47, 493, 651
BstC8I	GCNNGC	3 cut(s)	135, 709, 719
BstDEI	CTNAG	4 cut(s)	58, 138, 337, 692
BstDSI	CCRYGG	1 cut(s)	392
BstH2I	RGCGCY	1 cut(s)	300
BstHHI	GCGC	1 cut(s)	299
BstKTI	GATC	5 cut(s)	223, 249, 448, 526, 531
BstMAI	GTCTC	4 cut(s)	391, 482, 518, 582
BstMBI	GATC	5 cut(s)	220, 246, 445, 523, 528
BstNI	CCWGG	2 cut(s)	198, 422
BstNSI	RCATGY	2 cut(s)	107, 137
BstSCI	CCNGG	2 cut(s)	196, 420
BstV1I	GCAGC	1 cut(s)	573
BstX2I	RGATCY	1 cut(s)	528
BstYI	RGATCY	1 cut(s)	528
BtgI	CCRYGG	1 cut(s)	392
BtsIMutI	CAGTG	1 cut(s)	733
Cac8I	GCNNGC	3 cut(s)	135, 709, 719
CaiI	CAGNNNCTG	1 cut(s)	197
CciI	TCATGA	1 cut(s)	456
CfoI	GCGC	1 cut(s)	299
Cfr10I	RCCGGY	1 cut(s)	633
Cfr13I	GGNCC	3 cut(s)	254, 631, 681
CsiI	ACCWGGT	1 cut(s)	420
Csp6I	GTAC	1 cut(s)	553
CviAII	CATG	7 cut(s)	19, 104, 134, 213, 236, 457, 668
CviJI	RGCY	6 cut(s)	57, 69, 329, 564, 691, 711
CviKI_1	RGCY	6 cut(s)	57, 69, 329, 564, 691, 711
CviQI	GTAC	1 cut(s)	553
DdeI	CTNAG	4 cut(s)	58, 138, 337, 692
DpnI	GATC	5 cut(s)	222, 248, 447, 525, 530
DpnII	GATC	5 cut(s)	220, 246, 445, 523, 528
DraIII	CACNNNGTG	1 cut(s)	325
EciI	GGCGGA	1 cut(s)	652
Eco31I	GGTCTC	1 cut(s)	518
Eco47I	GGWCC	3 cut(s)	254, 631, 681
Eco47III	AGCGCT	1 cut(s)	298
Eco88I	CYCGRG	1 cut(s)	571
EcoRII	CCWGG	2 cut(s)	196, 420
Esp3I	CGTCTC	1 cut(s)	582
FaeI	CATG	7 cut(s)	22, 107, 137, 216, 239, 460, 671
FaqI	GGGAC	2 cut(s)	644, 694
FatI	CATG	7 cut(s)	18, 103, 133, 212, 235, 456, 667
FauI	CCCGC	1 cut(s)	55
Fnu4HI	GCNGC	1 cut(s)	562
Fsp4HI	GCNGC	1 cut(s)	562
GlaI	GCGC	1 cut(s)	298
GluI	GCNGC	1 cut(s)	562
HaeII	RGCGCY	1 cut(s)	300
HapII	CCGG	1 cut(s)	634
HhaI	GCGC	1 cut(s)	299
Hin1II	CATG	7 cut(s)	22, 107, 137, 216, 239, 460, 671
Hin6I	GCGC	1 cut(s)	297
HinP1I	GCGC	1 cut(s)	297
HinfI	GANTC	1 cut(s)	128
HpaII	CCGG	1 cut(s)	634
HphI	GGTGA	1 cut(s)	497
Hpy166II	GTNNAC	2 cut(s)	109, 334
Hpy188I	TCNGA	4 cut(s)	193, 340, 412, 528
Hpy188III	TCNNGA	8 cut(s)	140, 257, 449, 457, 547, 571, 573, 585
Hpy8I	GTNNAC	2 cut(s)	109, 334
HpyAV	CCTTC	2 cut(s)	276, 341
HpyCH4III	ACNGT	3 cut(s)	47, 493, 651
HpyCH4V	TGCA	3 cut(s)	292, 561, 654
HpyF3I	CTNAG	4 cut(s)	58, 138, 337, 692
Hsp92II	CATG	7 cut(s)	22, 107, 137, 216, 239, 460, 671
HspAI	GCGC	1 cut(s)	297
Kzo9I	GATC	5 cut(s)	220, 246, 445, 523, 528
Lsp1109I	GCAGC	1 cut(s)	573
LweI	GCATC	1 cut(s)	705
MabI	ACCWGGT	1 cut(s)	420
MaeIII	GTNAC	2 cut(s)	461, 729
MalI	GATC	5 cut(s)	222, 248, 447, 525, 530
MboI	GATC	5 cut(s)	220, 246, 445, 523, 528
MflI	RGATCY	1 cut(s)	528
MluCI	AATT	3 cut(s)	169, 406, 470
MnlI	CCTC	6 cut(s)	16, 79, 460, 503, 620, 671
MslI	CAYNNNNRTG	1 cut(s)	240
MspA1I	CMGCKG	1 cut(s)	62
MspI	CCGG	1 cut(s)	634
MspR9I	CCNGG	2 cut(s)	198, 422
Mva1269I	GAATGC	1 cut(s)	392
MvaI	CCWGG	2 cut(s)	198, 422
NdeII	GATC	5 cut(s)	220, 246, 445, 523, 528
NlaIII	CATG	7 cut(s)	22, 107, 137, 216, 239, 460, 671
NlaIV	GGNNCC	3 cut(s)	11, 632, 682
NspI	RCATGY	2 cut(s)	107, 137
PaeI	GCATGC	1 cut(s)	137
PaeR7I	CTCGAG	1 cut(s)	571
PagI	TCATGA	1 cut(s)	456
PciI	ACATGT	1 cut(s)	103
PctI	GAATGC	1 cut(s)	392
PfeI	GAWTC	1 cut(s)	128
PflMI	CCANNNNNTGG	1 cut(s)	269
PkrI	GCNGC	1 cut(s)	563
PscI	ACATGT	1 cut(s)	103
Psp6I	CCWGG	2 cut(s)	196, 420
PspGI	CCWGG	2 cut(s)	196, 420
PspN4I	GGNNCC	3 cut(s)	11, 632, 682
PspPI	GGNCC	3 cut(s)	254, 631, 681
PstNI	CAGNNNCTG	1 cut(s)	197
PsuI	RGATCY	1 cut(s)	528
RsaI	GTAC	1 cut(s)	554
RsaNI	GTAC	1 cut(s)	553
RseI	CAYNNNNRTG	1 cut(s)	240
SatI	GCNGC	1 cut(s)	562
Sau3AI	GATC	5 cut(s)	220, 246, 445, 523, 528
Sau96I	GGNCC	3 cut(s)	254, 631, 681
ScrFI	CCNGG	2 cut(s)	198, 422
SexAI	ACCWGGT	1 cut(s)	420
SfaNI	GCATC	1 cut(s)	705
Sfr274I	CTCGAG	1 cut(s)	571
SinI	GGWCC	3 cut(s)	254, 631, 681
SlaI	CTCGAG	1 cut(s)	571
SmiMI	CAYNNNNRTG	1 cut(s)	240
SmlI	CTYRAG	2 cut(s)	476, 571
SmoI	CTYRAG	2 cut(s)	476, 571
SphI	GCATGC	1 cut(s)	137
Sse9I	AATT	3 cut(s)	169, 406, 470
SsiI	CCGC	2 cut(s)	62, 637
SspI	AATATT	1 cut(s)	205
StyD4I	CCNGG	2 cut(s)	196, 420
TaaI	ACNGT	3 cut(s)	47, 493, 651
TaqI	TCGA	3 cut(s)	508, 548, 572
TasI	AATT	3 cut(s)	169, 406, 470
TatI	WGTACW	1 cut(s)	552
TfiI	GAWTC	1 cut(s)	128
TseI	GCWGC	1 cut(s)	561
TspDTI	ATGAA	5 cut(s)	17, 114, 176, 201, 330
TspGWI	ACGGA	1 cut(s)	409
Van91I	CCANNNNNTGG	1 cut(s)	269
VpaK11BI	GGWCC	3 cut(s)	254, 631, 681
XapI	RAATTY	1 cut(s)	406
XceI	RCATGY	2 cut(s)	107, 137
XhoI	CTCGAG	1 cut(s)	571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.