MD11G1198000.v1.1

E3 ubiquitin ligase BIG BROTHER-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010546
Physical Location & Seq
Reverse (-)
28517183 .. 28522739
5557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1198000.v1.1.491

Sequence Viewer

Length: 759 bp
ATGAGTTGGGACCCGAACATGGAAGTTCATTACACCTACACCAGCTGTCCTTATAGCTCAGCGGGTAGCTTTATGGAGTATTTTGAGGGTCTTACCTATGAACATGTGAACTTTATTTTTTCAGGCGATTCGCATGCTCAGGAGAGTGCTTACCCAACAATGAATACAAATTATTACAAGTTTGGGTTATCGGAACCTGGGAATACTTCATCCTATGATCTTGGGTTTAGTCATGCTTATGAGATCAATGATCCTGACCCAAGACGTGGTGAACAAAGAAGGCATTTGCAGAGCGCTTCAACAATGACTAATGAACAGAATGTGGCAGTGAACTCAGAATGGGAAGGAAATGCAAATACTTCTACAGGCGACAACCCCATAGAATGCCCACGGAGACATCAAAATTCCCATGATCATCAGGTTATTTGGCAAGACAACATTGATCCTGACAGCATGAGTTACGAGGAATTACTTGAGTTAGGTGAGGCAGTTGGAACTCAAAGTCGAGGTCTCTCCCAAGATCAGATCTCCTTGCTTCCAATCTCAAAGTACAAATGCAGCTTTTTCTCGAGAAAGAAATCACGAGACGAGAGATGTGTGATTTGCCAGATGGAATATAAACGAGGGGACCGGCGGATTGCTCTACCGTGCAAACACCTCTATCATGCTGGTTGTGGGACCAGATGGCTTAGCATCAACAAGGCTTGCCCAATCTGTTACACCAAGGTGTTTGGTGACGCGTCAAAGTGCGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

253

Amino Acids

28.77

Weight (kDa)

5.8

Isoelectric Point (pI)

52.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 193 - 239 9.9e-07 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 196 - 243 8.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 198 - 239 1e-12 Ring finger domain
zf-C3HC4_2 PF13923 198 - 239 2.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 199 - 225 3.6e-07 RING-like zinc finger
zf-C3HC4 PF00097 199 - 239 2.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 751
AccB7I CCANNNNNTGG 1 cut(s) 266
AccII CGCG 1 cut(s) 740
AciI CCGC 2 cut(s) 62, 634
AclWI GGATC 2 cut(s) 245, 437
AcsI RAATTY 1 cut(s) 403
AfaI GTAC 1 cut(s) 551
AfeI AGCGCT 1 cut(s) 295
AfiI CCNNNNNNNGG 2 cut(s) 19, 266
AflIII ACRYGT 2 cut(s) 103, 738
AgsI TTSAA 1 cut(s) 300
AjiI CACGTC 1 cut(s) 266
AjnI CCWGG 1 cut(s) 196
AleI CACNNNNGTG 1 cut(s) 725
AluBI AGCT 4 cut(s) 45, 57, 69, 561
AluI AGCT 4 cut(s) 45, 57, 69, 561
Alw26I GTCTC 3 cut(s) 388, 515, 579
AlwI GGATC 2 cut(s) 245, 437
Ama87I CYCGRG 1 cut(s) 568
Aor51HI AGCGCT 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 558
ApoI RAATTY 1 cut(s) 403
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
AspLEI GCGC 2 cut(s) 296, 752
AspS9I GGNCC 3 cut(s) 10, 628, 678
AsuHPI GGTGA 3 cut(s) 281, 494, 746
AvaI CYCGRG 1 cut(s) 568
AvaII GGWCC 3 cut(s) 10, 628, 678
BauI CACGAG 1 cut(s) 582
BbvI GCAGC 1 cut(s) 570
BccI CCATC 2 cut(s) 604, 678
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 1 cut(s) 198
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 3 cut(s) 388, 515, 579
BfmI CTRYAG 1 cut(s) 363
BfoI RGCGCY 1 cut(s) 297
BglII AGATCT 1 cut(s) 525
BisI GCNGC 1 cut(s) 559
BlpI GCTNAGC 2 cut(s) 58, 689
BlsI GCNGC 1 cut(s) 560
Bme1390I CCNGG 1 cut(s) 198
Bme18I GGWCC 3 cut(s) 10, 628, 678
BmeT110I CYCGRG 1 cut(s) 568
BmgBI CACGTC 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 10, 628, 678
BmiI GGNNCC 5 cut(s) 11, 12, 195, 629, 679
BmrFI CCNGG 1 cut(s) 198
BmsI GCATC 1 cut(s) 702
Bpu10I CCTNAGC 1 cut(s) 138
Bpu1102I GCTNAGC 2 cut(s) 58, 689
BpuEI CTTGAG 1 cut(s) 494
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 3 cut(s) 197, 389, 723
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 266
Bse118I RCCGGY 1 cut(s) 630
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 197, 389, 723
BseGI GGATG 1 cut(s) 209
BseLI CCNNNNNNNGG 2 cut(s) 19, 266
BseMII CTCAG 3 cut(s) 72, 152, 348
BseXI GCAGC 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 740
BsiHKCI CYCGRG 1 cut(s) 568
BsiSI CCGG 1 cut(s) 631
BslFI GGGAC 3 cut(s) 23, 641, 691
BslI CCNNNNNNNGG 2 cut(s) 19, 266
BsmAI GTCTC 3 cut(s) 388, 515, 579
BsmBI CGTCTC 1 cut(s) 579
BsmFI GGGAC 3 cut(s) 23, 641, 691
BsmI GAATGC 1 cut(s) 389
Bso31I GGTCTC 1 cut(s) 515
BsoBI CYCGRG 1 cut(s) 568
Bsp143I GATC 7 cut(s) 217, 243, 250, 412, 442, 520, 525
Bsp1720I GCTNAGC 2 cut(s) 58, 689
BspACI CCGC 2 cut(s) 62, 634
BspCNI CTCAG 3 cut(s) 71, 151, 347
BspFNI CGCG 1 cut(s) 740
BspLI GGNNCC 5 cut(s) 11, 12, 195, 629, 679
BspPI GGATC 2 cut(s) 245, 437
BspTNI GGTCTC 1 cut(s) 515
BsrFI RCCGGY 1 cut(s) 630
BssAI RCCGGY 1 cut(s) 630
BssECI CCNNGG 3 cut(s) 197, 389, 723
BssMI GATC 7 cut(s) 217, 243, 250, 412, 442, 520, 525
BssSI CACGAG 1 cut(s) 582
BssT1I CCWWGG 1 cut(s) 723
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 648
BstC8I GCNNGC 2 cut(s) 135, 706
BstDEI CTNAG 4 cut(s) 58, 138, 334, 689
BstDSI CCRYGG 1 cut(s) 389
BstF5I GGATG 1 cut(s) 209
BstFNI CGCG 1 cut(s) 740
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 2 cut(s) 296, 752
BstKTI GATC 7 cut(s) 220, 246, 253, 415, 445, 523, 528
BstMAI GTCTC 3 cut(s) 388, 515, 579
BstMBI GATC 7 cut(s) 217, 243, 250, 412, 442, 520, 525
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 2 cut(s) 107, 137
BstSCI CCNGG 1 cut(s) 196
BstSFI CTRYAG 1 cut(s) 363
BstUI CGCG 1 cut(s) 740
BstV1I GCAGC 1 cut(s) 570
BstX2I RGATCY 1 cut(s) 525
BstYI RGATCY 1 cut(s) 525
BtgI CCRYGG 1 cut(s) 389
BtrI CACGTC 1 cut(s) 266
BtsCI GGATG 1 cut(s) 209
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 2 cut(s) 135, 706
CfoI GCGC 2 cut(s) 296, 752
Cfr10I RCCGGY 1 cut(s) 630
Cfr13I GGNCC 3 cut(s) 10, 628, 678
CseI GACGC 2 cut(s) 729, 746
Csp6I GTAC 1 cut(s) 550
CviAII CATG 7 cut(s) 19, 104, 134, 233, 410, 454, 665
CviJI RGCY 6 cut(s) 45, 57, 69, 561, 688, 704
CviKI_1 RGCY 6 cut(s) 45, 57, 69, 561, 688, 704
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 4 cut(s) 58, 138, 334, 689
DpnI GATC 7 cut(s) 219, 245, 252, 414, 444, 522, 527
DpnII GATC 7 cut(s) 217, 243, 250, 412, 442, 520, 525
EciI GGCGGA 1 cut(s) 649
Eco130I CCWWGG 1 cut(s) 723
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 3 cut(s) 10, 628, 678
Eco47III AGCGCT 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 568
EcoO109I RGGNCCY 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 723
Esp3I CGTCTC 1 cut(s) 579
FaeI CATG 7 cut(s) 22, 107, 137, 236, 413, 457, 668
FaqI GGGAC 3 cut(s) 23, 641, 691
FatI CATG 7 cut(s) 18, 103, 133, 232, 409, 453, 664
FauI CCCGC 1 cut(s) 55
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 1 cut(s) 559
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 1 cut(s) 559
FspI TGCGCA 1 cut(s) 751
GlaI GCGC 2 cut(s) 295, 751
GluI GCNGC 1 cut(s) 559
HaeII RGCGCY 1 cut(s) 297
HapII CCGG 1 cut(s) 631
HgaI GACGC 2 cut(s) 729, 746
HhaI GCGC 2 cut(s) 296, 752
Hin1II CATG 7 cut(s) 22, 107, 137, 236, 413, 457, 668
Hin6I GCGC 2 cut(s) 294, 750
HinP1I GCGC 2 cut(s) 294, 750
HinfI GANTC 1 cut(s) 128
HpaII CCGG 1 cut(s) 631
HphI GGTGA 3 cut(s) 281, 494, 746
Hpy166II GTNNAC 3 cut(s) 109, 272, 331
Hpy188I TCNGA 3 cut(s) 193, 337, 525
Hpy188III TCNNGA 6 cut(s) 140, 254, 446, 568, 570, 582
Hpy8I GTNNAC 3 cut(s) 109, 272, 331
HpyAV CCTTC 2 cut(s) 273, 338
HpyCH4III ACNGT 1 cut(s) 648
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 4 cut(s) 289, 353, 558, 651
HpyF3I CTNAG 4 cut(s) 58, 138, 334, 689
HpySE526I ACGT 1 cut(s) 265
Hsp92II CATG 7 cut(s) 22, 107, 137, 236, 413, 457, 668
HspAI GCGC 2 cut(s) 294, 750
KflI GGGWCCC 1 cut(s) 10
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 7 cut(s) 217, 243, 250, 412, 442, 520, 525
Lsp1109I GCAGC 1 cut(s) 570
LweI GCATC 1 cut(s) 702
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 3 cut(s) 458, 716, 734
MalI GATC 7 cut(s) 219, 245, 252, 414, 444, 522, 527
MboI GATC 7 cut(s) 217, 243, 250, 412, 442, 520, 525
MflI RGATCY 1 cut(s) 525
MluCI AATT 3 cut(s) 169, 403, 467
MluI ACGCGT 1 cut(s) 738
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 6 cut(s) 79, 457, 478, 500, 617, 668
MslI CAYNNNNRTG 2 cut(s) 237, 725
MspA1I CMGCKG 2 cut(s) 45, 62
MspI CCGG 1 cut(s) 631
MspR9I CCNGG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 1 cut(s) 198
MvnI CGCG 1 cut(s) 740
NdeII GATC 7 cut(s) 217, 243, 250, 412, 442, 520, 525
NlaIII CATG 7 cut(s) 22, 107, 137, 236, 413, 457, 668
NlaIV GGNNCC 5 cut(s) 11, 12, 195, 629, 679
NmuCI GTSAC 1 cut(s) 734
NsbI TGCGCA 1 cut(s) 751
NspI RCATGY 2 cut(s) 107, 137
OliI CACNNNNGTG 1 cut(s) 725
PaeI GCATGC 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 568
PciI ACATGT 1 cut(s) 103
PctI GAATGC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 128
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 10
PscI ACATGT 1 cut(s) 103
Psp5II RGGWCCY 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 5 cut(s) 11, 12, 195, 629, 679
PspPI GGNCC 3 cut(s) 10, 628, 678
PspPPI RGGWCCY 1 cut(s) 10
PsuI RGATCY 1 cut(s) 525
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 2 cut(s) 237, 725
SatI GCNGC 1 cut(s) 559
Sau3AI GATC 7 cut(s) 217, 243, 250, 412, 442, 520, 525
Sau96I GGNCC 3 cut(s) 10, 628, 678
ScrFI CCNGG 1 cut(s) 198
SfaNI GCATC 1 cut(s) 702
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 568
SinI GGWCC 3 cut(s) 10, 628, 678
SlaI CTCGAG 1 cut(s) 568
SmiMI CAYNNNNRTG 2 cut(s) 237, 725
SmlI CTYRAG 2 cut(s) 473, 568
SmoI CTYRAG 2 cut(s) 473, 568
SphI GCATGC 1 cut(s) 137
Sse9I AATT 3 cut(s) 169, 403, 467
SsiI CCGC 2 cut(s) 62, 634
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 1 cut(s) 648
TaiI ACGT 1 cut(s) 268
TaqI TCGA 2 cut(s) 505, 569
TasI AATT 3 cut(s) 169, 403, 467
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 1 cut(s) 128
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 1 cut(s) 558
Tsp45I GTSAC 1 cut(s) 734
TspDTI ATGAA 5 cut(s) 17, 114, 176, 198, 327
TspGWI ACGGA 1 cut(s) 406
TspRI CASTG 1 cut(s) 333
Van91I CCANNNNNTGG 1 cut(s) 266
VpaK11BI GGWCC 3 cut(s) 10, 628, 678
XapI RAATTY 1 cut(s) 403
XceI RCATGY 2 cut(s) 107, 137
XhoI CTCGAG 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.