Rh5AG350000

E3 ubiquitin ligase BIG BROTHER-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010546
Physical Location & Seq
Reverse (-)
58006560 .. 58012053
5494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG350000.1

Sequence Viewer

Length: 813 bp
ATGAGTTGGAACCCGCATATGGGAGTTCATTACCCCTACAACAGCTGTCCCTATAGCACGGTGGGTAGCTTTATGGACTATGTTGAAGGCCTCACCTACGAACATGTGAACTTTATTTTTTCAGGGGATTCACATGCTCAGGAGAGCACTTACCCATCAATGAATACGAACTATTACAAGTTTGGGTTAACTGAACCTGGGAATACTTCATATTATGATCTTGGTTTTCGTCATTCCTATGAGTTCAATGATCCTATCTCAAGAAGTGGTGAAGAAACAGGGCCTTTGCAGTACTCTTCAACTATTCCTAATGAACAGAATGTGGCAAGGGATCCACCATGCGAAGGAAGTGCAAACACTTCCACATGTGACAACCCCAGAGAATCCCCTCGAAGATATCAGAACTCCCAGGATTATCAGGTTATTTTGGAAGACAACATTGACCCTGATAGCATGACTTACGAGGAATTACTAGAATTAGGTGAGGCAGTTGGAACTGAAAGCCGAGGTCTTACCCAAGATATGCTTTCTTTGCTTCCAATCTCGAAGTACAAGTGCAGCTTTTTCTCAAGAAAGAAATCACGGAGTGAGAGGTATAATTGCCCTTATTTATTGTTCTTTTGCGGAAATGTGATTACTCTCTCTCTCTCTCTAATAATTGAAAATCAATGGTACATTTATGGTTTGTTTTGCAGGAATAGCTTGCTCTTTTATCTATTTTTGAATAAACTTACCTGTAAAAGACAAGGAAAATGGTTTTTCATTGTTTTCATTCACACGATATACTGTAGGGACTTATTTGGGAAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

270

Amino Acids

31.32

Weight (kDa)

5.28

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 14, 624
AclWI GGATC 3 cut(s) 245, 326, 339
AdeI CACNNNGTG 1 cut(s) 587
AfaI GTAC 3 cut(s) 293, 551, 674
AfiI CCNNNNNNNGG 3 cut(s) 19, 20, 344
AflIII ACRYGT 2 cut(s) 103, 365
AgsI TTSAA 5 cut(s) 86, 247, 300, 662, 724
AjnI CCWGG 2 cut(s) 196, 408
AluBI AGCT 4 cut(s) 45, 69, 561, 702
AluI AGCT 4 cut(s) 45, 69, 561, 702
Alw21I GWGCWC 1 cut(s) 149
AlwI GGATC 3 cut(s) 245, 326, 339
AoxI GGCC 2 cut(s) 88, 281
ApeKI GCWGC 1 cut(s) 558
AspS9I GGNCC 1 cut(s) 281
AsuHPI GGTGA 3 cut(s) 85, 281, 494
BamHI GGATCC 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 570
BccI CCATC 1 cut(s) 163
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 2 cut(s) 198, 410
BfaI CTAG 1 cut(s) 473
BfmI CTRYAG 2 cut(s) 52, 787
BisI GCNGC 1 cut(s) 559
BlsI GCNGC 1 cut(s) 560
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 2 cut(s) 198, 410
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 2 cut(s) 11, 333
BmrFI CCNGG 2 cut(s) 198, 410
BpiI GAAGAC 1 cut(s) 438
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 2 cut(s) 244, 553
BsaJI CCNNGG 3 cut(s) 197, 408, 505
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 20, 344
BseBI CCWGG 2 cut(s) 198, 410
BseDI CCNNGG 3 cut(s) 197, 408, 505
BseLI CCNNNNNNNGG 3 cut(s) 19, 20, 344
BseMII CTCAG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 570
BsgI GTGCAG 1 cut(s) 577
BshFI GGCC 2 cut(s) 90, 283
BsiHKAI GWGCWC 1 cut(s) 149
BslFI GGGAC 2 cut(s) 33, 806
BslI CCNNNNNNNGG 3 cut(s) 19, 20, 344
BsmFI GGGAC 2 cut(s) 33, 806
BsnI GGCC 2 cut(s) 90, 283
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 3 cut(s) 217, 250, 331
BspACI CCGC 2 cut(s) 14, 624
BspANI GGCC 2 cut(s) 90, 283
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 11, 333
BspPI GGATC 3 cut(s) 245, 326, 339
BssECI CCNNGG 3 cut(s) 197, 408, 505
BssMI GATC 3 cut(s) 217, 250, 331
Bst2UI CCWGG 2 cut(s) 198, 410
Bst4CI ACNGT 2 cut(s) 61, 788
Bst6I CTCTTC 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 704
BstDEI CTNAG 1 cut(s) 138
BstKTI GATC 3 cut(s) 220, 253, 334
BstMBI GATC 3 cut(s) 217, 250, 331
BstMWI GCNNNNNNNGC 2 cut(s) 532, 699
BstNI CCWGG 2 cut(s) 198, 410
BstNSI RCATGY 3 cut(s) 107, 137, 369
BstSCI CCNGG 2 cut(s) 196, 408
BstSFI CTRYAG 2 cut(s) 52, 787
BstV1I GCAGC 1 cut(s) 570
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BsuRI GGCC 2 cut(s) 90, 283
Cac8I GCNNGC 1 cut(s) 704
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 3 cut(s) 292, 550, 673
CviAII CATG 5 cut(s) 104, 134, 339, 366, 454
CviJI RGCY 7 cut(s) 45, 69, 90, 283, 504, 561, 702
CviKI_1 RGCY 7 cut(s) 45, 69, 90, 283, 504, 561, 702
CviQI GTAC 3 cut(s) 292, 550, 673
DdeI CTNAG 1 cut(s) 138
DpnI GATC 3 cut(s) 219, 252, 333
DpnII GATC 3 cut(s) 217, 250, 331
DraIII CACNNNGTG 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 301
EarI CTCTTC 1 cut(s) 301
Eco147I AGGCCT 1 cut(s) 90
Eco32I GATATC 1 cut(s) 398
EcoO109I RGGNCCY 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 196, 408
EcoRV GATATC 1 cut(s) 398
FaeI CATG 5 cut(s) 107, 137, 342, 369, 457
FalI AAGNNNNNCTT 4 cut(s) 510, 542, 545, 577
FaqI GGGAC 2 cut(s) 33, 806
FatI CATG 5 cut(s) 103, 133, 338, 365, 453
FauI CCCGC 1 cut(s) 21
FauNDI CATATG 1 cut(s) 18
Fnu4HI GCNGC 1 cut(s) 559
Fsp4HI GCNGC 1 cut(s) 559
FspBI CTAG 1 cut(s) 473
GluI GCNGC 1 cut(s) 559
HaeIII GGCC 2 cut(s) 90, 283
Hin1II CATG 5 cut(s) 107, 137, 342, 369, 457
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 2 cut(s) 128, 383
HpaI GTTAAC 1 cut(s) 189
HphI GGTGA 3 cut(s) 85, 281, 494
Hpy166II GTNNAC 2 cut(s) 109, 189
Hpy188I TCNGA 1 cut(s) 402
Hpy188III TCNNGA 4 cut(s) 140, 261, 544, 570
Hpy8I GTNNAC 2 cut(s) 109, 189
HpyAV CCTTC 2 cut(s) 80, 338
HpyCH4III ACNGT 2 cut(s) 61, 788
HpyCH4V TGCA 4 cut(s) 289, 353, 558, 693
HpyF10VI GCNNNNNNNGC 2 cut(s) 532, 699
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 5 cut(s) 107, 137, 342, 369, 457
KspAI GTTAAC 1 cut(s) 189
Kzo9I GATC 3 cut(s) 217, 250, 331
Lsp1109I GCAGC 1 cut(s) 570
MaeI CTAG 1 cut(s) 473
MaeIII GTNAC 1 cut(s) 368
MalI GATC 3 cut(s) 219, 252, 333
MboI GATC 3 cut(s) 217, 250, 331
MboII GAAGA 4 cut(s) 284, 288, 405, 443
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 4 cut(s) 467, 476, 598, 657
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 6 cut(s) 101, 399, 457, 478, 500, 585
MseI TTAA 1 cut(s) 188
MslI CAYNNNNRTG 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 45
MspR9I CCNGG 2 cut(s) 198, 410
MvaI CCWGG 2 cut(s) 198, 410
MwoI GCNNNNNNNGC 2 cut(s) 532, 699
NdeI CATATG 1 cut(s) 18
NdeII GATC 3 cut(s) 217, 250, 331
NlaIII CATG 5 cut(s) 107, 137, 342, 369, 457
NlaIV GGNNCC 2 cut(s) 11, 333
NmeAIII GCCGAG 1 cut(s) 530
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 3 cut(s) 107, 137, 369
PceI AGGCCT 1 cut(s) 90
PciI ACATGT 2 cut(s) 103, 365
PfeI GAWTC 2 cut(s) 128, 383
PkrI GCNGC 1 cut(s) 560
PscI ACATGT 2 cut(s) 103, 365
Psp6I CCWGG 2 cut(s) 196, 408
PspGI CCWGG 2 cut(s) 196, 408
PspN4I GGNNCC 2 cut(s) 11, 333
PspPI GGNCC 1 cut(s) 281
PsuI RGATCY 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 45
RsaI GTAC 3 cut(s) 293, 551, 674
RsaNI GTAC 3 cut(s) 292, 550, 673
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 1 cut(s) 559
Sau3AI GATC 3 cut(s) 217, 250, 331
Sau96I GGNCC 1 cut(s) 281
ScaI AGTACT 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 198, 410
SduI GDGCHC 1 cut(s) 149
SfcI CTRYAG 2 cut(s) 52, 787
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 2 cut(s) 259, 568
SmoI CTYRAG 2 cut(s) 259, 568
Sse9I AATT 4 cut(s) 467, 476, 598, 657
SseBI AGGCCT 1 cut(s) 90
SsiI CCGC 2 cut(s) 14, 624
SspMI CTAG 1 cut(s) 473
StuI AGGCCT 1 cut(s) 90
StyD4I CCNGG 2 cut(s) 196, 408
TaaI ACNGT 2 cut(s) 61, 788
TaqI TCGA 2 cut(s) 391, 545
TasI AATT 4 cut(s) 467, 476, 598, 657
TatI WGTACW 2 cut(s) 291, 549
TfiI GAWTC 2 cut(s) 128, 383
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 1 cut(s) 558
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 6 cut(s) 17, 176, 198, 327, 751, 760
TspGWI ACGGA 1 cut(s) 598
XceI RCATGY 3 cut(s) 107, 137, 369
XspI CTAG 1 cut(s) 473
ZrmI AGTACT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.