FvH4_3g29670

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014702
Physical Location & Seq
Forward (+)
22703243 .. 22711225
7983 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29670.t1

Sequence Viewer

Length: 3975 bp
ATGAAGAAGCGGAGGTTTCGCGACTTTGCGGCTTGGTCTTCGACTAACGATCGCGATACGACGGAGGGAAACCGACGTCCGTACTCGGGATGGGTTTCGGGTTCGGACCGGACGGAAAATCTGGCCGGGAGAGAGTACTATGAAGACGGCGATACAGATAGGCGGCCGCTCAAACCGCCGCAGACGCATTTGAGCGGCGACTTGCGTCAATGTGAATCAATGTCGATGAAGTTTCAGTGGAACAATCTGCTGGCCCAGAATCCGAATCAGGGTTCTAACCTGAGGGTTCTGACCCGACCCGAGTTTGATTATAGCAATAGACTGGATTATGAAATTGGTTATGGGGGAAGGGGTTTGGGTGTGGACCAGATTAAGCAATATGGGGGTTTTGATCATGGTGGCTACACGTCACCATTGCCCAAATCAAATCTTGGTTTCCAAGAAAATGCGGAGGACTGCCGCCCAATCTCATATAGGGCTCTGGAAGAGGAGGCTAGCTTAACTTCTGGAAGTGGGTATTCATCAATGGCTGTGAAGGAGGGTTTTCACCGTGCAATGGCTGAGATGCCAATCGGCCATGGTGGATATGCTCGAGGTCACATGCCTAGATTCAATGAGTTGGTGGACTATGGAGGTTCATCAATTGCTAATGAGGGTTTTGGTTTCGGTGGCGAATTCATCAGAAGCAGAGAAACGGTTCATGGTGGATGTTCTCAAGGTCAAACAGTTAGACACAATGAGGTGGTAGGCAATGAGGCTTCATCAGAAGCTTTGACTGAGCGTTTTCACAGCAGAAGCAGAGAAAGGGGCCATGATGGATATTGTCAAGGTCTAACAGCGAGACGCATTGAGTTTGATGACAATGGTCCATCAGAAGCTTTGACTGAGGGTTTTTGCAGCAGAAGCAGAGCAATGGCTGAGGGGCCTGTTGGCCATGCCGAATGTTCTCTAGGATGCACTCCAACACACAACCATGGGAGTTTACCAATTGCTTTGAATGAGAGTTTTCGCACTTCAGATGAGTTTACCAAAAGCAGAGTAATGGCTGAGAGGCCTGTCGGCCATGCTGGATATTTTCAAGGTCAAACAACTAGACAGAGTGAGTTAGCAGACAATGCAGCTTCATCGGAAGCTTTGAATGAGGGTTTTCACAGCAGAAGCAGAGTAAGGGGTGAGGGGCCTGTTGGCCGTGCTGAATACTCTCGAGGACAGACACCAATACACAGCAATGGAAGTTCACCAATTGATTTGAATGAGGGTTTTCGAGCTTCAGATGCGTTTACCAACAGCAGAGCTATTGTTGAGGGTCCTATTGGCCATGGTGGATATTCCTGTGGTAGAAGCAGAACAATGGCTGAGGGGCCTGTTAGCTGTGCTGGATATTCTGGAGGACATACACCAAGACACAACCATAGGAATTCACCAATTGCTTTGAATGAGGCTTTTTGGGCTGGAGATGAGGTTAACAAGAGCAGAGAAATGGCTGAGGGATCTGTTGGCCACGGTGGATATTCTCATGATCAAACACCAAGAAAGAATGAGTTGGTGCACCCTAGGTGGGCACAGCAGAGCCCAGAAAAGGAAATGAGTAGGGACCACAAGGGTTCTGGAATGAAAAGAAATGACAATGGTGATATGAATGCCTTGTTCCTTCTCGACTTACTCTATACGAAGATGGCTAGTGTTGGAGAAGACCCAAGGTCTCCTAGTCATTTGAGGCTGACTGTTACGGATTCTGTTGTGCAGAGGGTTGGTGGTAGCAATGGCTCTGTTGGAGCAAGCACTCATGACAATTGCCAGTTGGAACAATATCATGATGATATGGAACCAACAAATATGTTTCCCAATCATCAATCTACAAGTGAACCATCAATCCCTCATGATGAATTTTCGGAGTCAAGAACAGAGGTATCTTGGAACAATGAAATCCACGGCTTCAGATTAGGAGTAGATGTTGGTCATGAAAGAGAGGTCGAGCCTTTATCATATGAAGGCAACGTACAGACATCAGCCCTGCCTGATGAAGAACAAGGCTTGCTCGAGGCTTATGGTGGTTGCCAACAGAGGTTGGCAGAAGCCGAAAGCCTGGCATCCATGACCCCTGTGAAAAGGTGGGCATTGAAAAAAGAAGCCATGAAGCAGCAACAATCCGGCAATCACTCAAAGTCTATGTTCTTCCCTGATTTGAACCCCTCTAGTAATGTACTGAACTCGAGCTATGAAGTCGACATATCGTTGACTACTACTGAAGATATTCAGCAGCAATTTGCTAATGAAGATATTCAACAATATGCTGCTGCCTGTGGAGATGACCAATGGAGGATGGAGCAAAATCTGATATGTGGAGATGATCAATGGAACATTGATCAAGGTGTGACCCGTGAAGTGGATCAAGGGAACATTGCAGAAGGTTCAATTGACCAAGTTGTGACCTGTGGAGTGGATCAAAGGAGCATTGCACAAGGTGTGACCTCTGGAGAGGATCAATGTAACATCCATCGAGGCGTGATCTCCGGAGTGGATCAATGGAACAATGATCAAGGTGCGACAGCCTATGGCGAGCAACTTTGGGGTGTGAATAATGGAAAGGACCAACAGAGCAATGATAAAGGCCTTACAACCTGTGGAGAACAACTGTGGCGCATGAACTATGGAGAGGACCAATGGAATGCTGAGCATATCAACAACCTGAATTCTGAATTGCCAGCATCTATGCAAGAACATTTTGGCGAACAATCAGAAGCTAGGATACATGTAAAGGAGCGTTTGGGACCTCCCAAATTATCAACTGGAAACATAAAGACTCGTTTGGGACCTCCCAGATTATCACCTGGAAACATAAAGACTTGTTTGGGACCACCTATGAAATCACCTGGCAGTGTGAAGATGCGTTTGGGGCCTTCTCCAAATGATCCTCGTGTCAACATCTCAGGTTGCAAACCTCCAGAACAATACAAATCTCGGATAATAAAAATAGATGGTTTCGGTGAAAGCATTCATCCTAAAGGGGATGTCTTTATAAAAGTTAAGACAGATGCTGTTACTGAGAACATTCATCCTCAGAAGAGTGCTCTTCCACAAGTCGAGATGGACAGTCTTTCAAAAGTTAAGCCAGCTGTTACCGGAAGCAGTCATCCTCATGGGAAGACTCTTCCAAATGTTAAGAAAGGTGATTGTAGTGGAAGCATTTGTCCTGAAGGGGATAGTCATTCAAAAGTTAATGTGGAAGCTGCAAAGAAAGAGCCCTCTGAAGACTCTAAGGAGTTCAAGCGGATGATAGATGATGCCTTTATTAAGTTCATTAAAATTTTGATTGAGAACCCAACTCAAAGAAGACAATTCATGGAAGGAAGAGGAGGGAAGTGTTGCGTCTGTGCCAGCAATAATGAGTTTGTAGAAACGGAAACCATAGCAAGGCATGCCTTCAGCTCACCAAAGATTGGTTACAGAGCAGAGCACTTGGGCTTTCATAAAGCACTTTGTGTGCTCTTGGGATGGGACAGTGCAGCAGTCTCGAATGGTAGGTGGGTTCAGCAGAAGCTGCCGGATGCAAAAGCCTTGGCTTTGAAGGAGGACCTAGTTATCTGGCCTCCTGTTGTTGTCATACACAATAGTTCCTTAGCGAATGAAAAGCCTGAAGGTGGAAGGCAGACGGTAATGGTTGAAGGTCTTGAGGCCGTACTAACAGGGATGGGATTGGTTGGGGGGAAGACCAATGTGTACTGTGGGGAACCTGCCGATGGGAGTGTCATGGTGGTGAAGTTCAAGCCTACGTATTCTGGTTTGCAAGAAGCAGAAAGGCTTCACAAGCTGTTTGCCGATAACAAACATGGGAAGGCTGAGTGGTGGAATACCAGTTCTCTTCGCCTCAGCAACAGCAGTAATGCAAAGCAAGATCTAGAGCATGACAAAGCAAATTATCCCTATGGTTATTTGGGAAATGCAGAGGATTATGGGGTTGATCCTTTGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1325

Amino Acids

145.59

Weight (kDa)

5.75

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 1191 - 1318 6.9e-17 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014702)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g29670
malus_domestica MD03G1136700.v1.1
prunus_persica Prupe.6G121100_v2.0.a1 Prupe.6G121100_v2.0.a1
pyrus_communis pycom03g09250
rosa_chinensis RchiOBHm_Chr5g0055031
rosa_laevigata RLG00000034958
rosa_multiflora Rmu_co8511567.1_g000001 Rmu_sc0000925.1_g000013
rosa_roxburghii Rroxscaffold_1G00025460
rosa_rugosa Rorug05G0290200 Rorug05G0290300
rosa_samantha Rh5AG360200 Rh5BG371200 Rh5CG393500 Rh5DG385000
rosa_wichuraiana Rw5G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3020
AatII GACGTC 1 cut(s) 79
Acc36I ACCTGC 1 cut(s) 3742
AccB7I CCANNNNNTGG 1 cut(s) 3440
AccBSI CCGCTC 2 cut(s) 169, 195
AccI GTMKAC 1 cut(s) 2226
AccII CGCG 2 cut(s) 21, 54
AccIII TCCGGA 1 cut(s) 2513
AclWI GGATC 7 cut(s) 1498, 2397, 2451, 2490, 2529, 2906, 3956
AcoI YGGCCR 8 cut(s) 123, 164, 574, 931, 1060, 1186, 1315, 1498
AcsI RAATTY 5 cut(s) 674, 1417, 1886, 2692, 3306
AcuI CTGAAG 8 cut(s) 999, 1254, 1921, 2268, 3216, 3270, 3409, 3657
AcyI GRCGYC 1 cut(s) 76
AdeI CACNNNGTG 2 cut(s) 2465, 3482
AfaI GTAC 6 cut(s) 83, 137, 2001, 2205, 3681, 3722
AflIII ACRYGT 2 cut(s) 405, 2752
AhdI GACNNNNNGTC 1 cut(s) 1699
AjiI CACGTC 1 cut(s) 408
AjnI CCWGG 3 cut(s) 2085, 2830, 2872
AjuI GAANNNNNNNTTGG 2 cut(s) 2876, 2908
AloI GAACNNNNNNTCC 4 cut(s) 1857, 1889, 3841, 3873
Alw21I GWGCWC 4 cut(s) 1551, 3073, 3459, 3489
Alw26I GTCTC 3 cut(s) 835, 1707, 3517
Alw44I GTGCAC 1 cut(s) 1547
AlwI GGATC 7 cut(s) 1498, 2397, 2451, 2490, 2529, 2906, 3956
AlwNI CAGNNNCTG 2 cut(s) 3038, 3541
Ama87I CYCGRG 6 cut(s) 85, 299, 591, 1203, 2039, 2212
Aor13HI TCCGGA 1 cut(s) 2513
ApaLI GTGCAC 1 cut(s) 1547
ApeKI GCWGC 9 cut(s) 897, 1118, 2140, 2260, 2294, 2297, 3230, 3506, 3541
ApoI RAATTY 5 cut(s) 674, 1417, 1886, 2692, 3306
Asp700I GAANNNNTTC 5 cut(s) 696, 2253, 2280, 2994, 3801
AspA2I CCTAGG 1 cut(s) 1553
AspLEI GCGC 1 cut(s) 2643
AsuC2I CCSGG 1 cut(s) 127
AsuNHI GCTAGC 1 cut(s) 494
AvaI CYCGRG 6 cut(s) 85, 299, 591, 1203, 2039, 2212
AvrII CCTAGG 1 cut(s) 1553
AxyI CCTNAGG 1 cut(s) 281
BaeGI GKGCMC 2 cut(s) 1551, 1564
BaeI ACNNNNGTAYC 2 cut(s) 1893, 1926
BalI TGGCCA 3 cut(s) 933, 1317, 1500
BanII GRGCYC 3 cut(s) 481, 1574, 3246
BarI GAAGNNNNNNTAC 4 cut(s) 1983, 2015, 2733, 2765
BauI CACGAG 1 cut(s) 2916
BbsI GAAGAC 7 cut(s) 30, 150, 1698, 3152, 3258, 3340, 3716
Bbv12I GWGCWC 4 cut(s) 1551, 3073, 3459, 3489
BbvCI CCTCAGC 4 cut(s) 918, 1356, 1485, 3869
BbvI GCAGC 9 cut(s) 909, 1130, 2152, 2272, 2281, 2284, 3217, 3518, 3528
BceAI ACGGC 4 cut(s) 163, 1173, 1948, 3662
BcgI CGANNNNNNTGC 4 cut(s) 1107, 1141, 3495, 3529
BciT130I CCWGG 3 cut(s) 2087, 2832, 2874
BciVI GTATCC 1 cut(s) 2742
BclI TGATCA 5 cut(s) 391, 1519, 2350, 2365, 2536
BcnI CCSGG 1 cut(s) 127
BcoDI GTCTC 3 cut(s) 835, 1707, 3517
BfuAI ACCTGC 1 cut(s) 3742
BfuI GTATCC 1 cut(s) 2742
BglII AGATCT 1 cut(s) 3895
BlnI CCTAGG 1 cut(s) 1553
BlpI GCTNAGC 1 cut(s) 2673
BmcAI AGTACT 1 cut(s) 137
Bme1390I CCNGG 4 cut(s) 127, 2087, 2832, 2874
BmeRI GACNNNNNGTC 1 cut(s) 1699
BmeT110I CYCGRG 6 cut(s) 85, 299, 591, 1203, 2039, 2212
BmgBI CACGTC 1 cut(s) 408
BmrFI CCNGG 4 cut(s) 127, 2087, 2832, 2874
BmsI GCATC 9 cut(s) 555, 944, 1264, 2099, 2717, 2877, 3025, 3274, 3538
BmtI GCTAGC 1 cut(s) 498
BoxI GACNNNNGTC 2 cut(s) 204, 864
BpiI GAAGAC 7 cut(s) 30, 150, 1698, 3152, 3258, 3340, 3716
BpmI CTGGAG 4 cut(s) 1407, 1473, 2496, 2928
Bpu10I CCTNAGC 5 cut(s) 918, 1356, 1485, 3619, 3869
Bpu1102I GCTNAGC 1 cut(s) 2673
BpuEI CTTGAG 2 cut(s) 699, 3692
BpuMI CCSGG 1 cut(s) 127
BsaAI YACGTR 1 cut(s) 3774
BsaBI GATNNNNATC 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 76
BsaI GGTCTC 1 cut(s) 1707
BsaJI CCNNGG 8 cut(s) 577, 973, 1318, 1501, 1553, 1697, 1930, 3558
BsaWI WCCGGW 3 cut(s) 108, 2513, 3122
BsaXI ACNNNNNCTCC 4 cut(s) 1446, 1476, 1938, 1968
Bse1I ACTGG 4 cut(s) 327, 1798, 2794, 3855
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 9 cut(s) 413, 561, 757, 918, 1234, 1768, 2400, 2454, 2608
Bse8I GATNNNNATC 1 cut(s) 569
BseAI TCCGGA 1 cut(s) 2513
BseBI CCWGG 3 cut(s) 2087, 2832, 2874
BseDI CCNNGG 8 cut(s) 577, 973, 1318, 1501, 1553, 1697, 1930, 3558
BseJI GATNNNNATC 1 cut(s) 569
BseMI GCAATG 9 cut(s) 413, 561, 757, 918, 1234, 1768, 2400, 2454, 2608
BseNI ACTGG 4 cut(s) 327, 1798, 2794, 3855
BseRI GAGGAG 2 cut(s) 503, 3369
BseSI GKGCMC 2 cut(s) 1551, 1564
BseX3I CGGCCG 1 cut(s) 164
BseXI GCAGC 9 cut(s) 909, 1130, 2152, 2272, 2281, 2284, 3217, 3518, 3528
BsgI GTGCAG 2 cut(s) 1763, 3525
Bsh1236I CGCG 2 cut(s) 21, 54
Bsh1285I CGRYCG 2 cut(s) 52, 167
BsiEI CGRYCG 2 cut(s) 52, 167
BsiHKAI GWGCWC 4 cut(s) 1551, 3073, 3459, 3489
BsiHKCI CYCGRG 6 cut(s) 85, 299, 591, 1203, 2039, 2212
BsiSI CCGG 6 cut(s) 109, 126, 2151, 2514, 3123, 3545
BslFI GGGAC 5 cut(s) 1607, 2784, 2826, 2868, 3512
BsmAI GTCTC 3 cut(s) 835, 1707, 3517
BsmBI CGTCTC 1 cut(s) 835
BsmFI GGGAC 5 cut(s) 1607, 2784, 2826, 2868, 3512
BsmI GAATGC 3 cut(s) 1645, 2674, 2994
Bso31I GGTCTC 1 cut(s) 1707
BsoBI CYCGRG 6 cut(s) 85, 299, 591, 1203, 2039, 2212
Bsp1286I GDGCHC 8 cut(s) 481, 1551, 1564, 1574, 3073, 3246, 3459, 3489
Bsp13I TCCGGA 1 cut(s) 2513
Bsp1720I GCTNAGC 1 cut(s) 2673
Bsp19I CCATGG 3 cut(s) 577, 973, 1318
Bsp68I TCGCGA 2 cut(s) 21, 54
BspEI TCCGGA 1 cut(s) 2513
BspFNI CGCG 2 cut(s) 21, 54
BspHI TCATGA 5 cut(s) 1516, 1786, 1813, 1879, 1960
BspMI ACCTGC 1 cut(s) 3742
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 7 cut(s) 1498, 2397, 2451, 2490, 2529, 2906, 3956
BspQI GCTCTTC 1 cut(s) 3078
BspTNI GGTCTC 1 cut(s) 1707
BsrBI CCGCTC 2 cut(s) 169, 195
BsrDI GCAATG 9 cut(s) 413, 561, 757, 918, 1234, 1768, 2400, 2454, 2608
BsrI ACTGG 4 cut(s) 327, 1798, 2794, 3855
BssECI CCNNGG 8 cut(s) 577, 973, 1318, 1501, 1553, 1697, 1930, 3558
BssNI GRCGYC 1 cut(s) 76
BssSI CACGAG 1 cut(s) 2916
BssT1I CCWWGG 6 cut(s) 577, 973, 1318, 1553, 1697, 3558
Bst2BI CACGAG 1 cut(s) 2916
Bst2UI CCWGG 3 cut(s) 2087, 2832, 2874
Bst6I CTCTTC 6 cut(s) 480, 3059, 3078, 3156, 3346, 3867
BstACI GRCGYC 1 cut(s) 76
BstAPI GCANNNNNTGC 3 cut(s) 1115, 3419, 3541
BstBAI YACGTR 1 cut(s) 3774
BstC8I GCNNGC 9 cut(s) 252, 496, 1780, 2036, 2561, 2706, 3114, 3379, 3420
BstDSI CCRYGG 5 cut(s) 577, 973, 1318, 1501, 1930
BstFNI CGCG 2 cut(s) 21, 54
BstHHI GCGC 1 cut(s) 2643
BstMAI GTCTC 3 cut(s) 835, 1707, 3517
BstMCI CGRYCG 2 cut(s) 52, 167
BstNI CCWGG 3 cut(s) 2087, 2832, 2874
BstNSI RCATGY 3 cut(s) 604, 2756, 3422
BstPAI GACNNNNGTC 2 cut(s) 204, 864
BstSCI CCNGG 4 cut(s) 125, 2085, 2830, 2872
BstSLI GKGCMC 2 cut(s) 1551, 1564
BstSNI TACGTA 1 cut(s) 3774
BstUI CGCG 2 cut(s) 21, 54
BstV1I GCAGC 9 cut(s) 909, 1130, 2152, 2272, 2281, 2284, 3217, 3518, 3528
BstV2I GAAGAC 7 cut(s) 30, 150, 1698, 3152, 3258, 3340, 3716
BstX2I RGATCY 2 cut(s) 1490, 3895
BstYI RGATCY 2 cut(s) 1490, 3895
BstZI CGGCCG 1 cut(s) 164
Bsu36I CCTNAGG 1 cut(s) 281
BsuI GTATCC 1 cut(s) 2742
BtgI CCRYGG 5 cut(s) 577, 973, 1318, 1501, 1930
BtrI CACGTC 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 2884
BtsIMutI CAGTG 3 cut(s) 242, 2884, 3508
BtuMI TCGCGA 2 cut(s) 21, 54
BveI ACCTGC 1 cut(s) 3742
Cac8I GCNNGC 9 cut(s) 252, 496, 1780, 2036, 2561, 2706, 3114, 3379, 3420
CaiI CAGNNNCTG 2 cut(s) 3038, 3541
CciI TCATGA 5 cut(s) 1516, 1786, 1813, 1879, 1960
CciNI GCGGCCGC 1 cut(s) 164
CfoI GCGC 1 cut(s) 2643
CpoI CGGWCCG 1 cut(s) 106
CseI GACGC 4 cut(s) 193, 194, 852, 3358
Csp6I GTAC 6 cut(s) 82, 136, 2000, 2204, 3680, 3721
CspI CGGWCCG 1 cut(s) 106
CviQI GTAC 6 cut(s) 82, 136, 2000, 2204, 3680, 3721
DraIII CACNNNGTG 2 cut(s) 2465, 3482
DriI GACNNNNNGTC 1 cut(s) 1699
EaeI YGGCCR 8 cut(s) 123, 164, 574, 931, 1060, 1186, 1315, 1498
EagI CGGCCG 1 cut(s) 164
Eam1104I CTCTTC 6 cut(s) 480, 3059, 3078, 3156, 3346, 3867
Eam1105I GACNNNNNGTC 1 cut(s) 1699
EarI CTCTTC 6 cut(s) 480, 3059, 3078, 3156, 3346, 3867
EclXI CGGCCG 1 cut(s) 164
Eco105I TACGTA 1 cut(s) 3774
Eco130I CCWWGG 6 cut(s) 577, 973, 1318, 1553, 1697, 3558
Eco147I AGGCCT 2 cut(s) 1054, 2613
Eco24I GRGCYC 3 cut(s) 481, 1574, 3246
Eco31I GGTCTC 1 cut(s) 1707
Eco52I CGGCCG 1 cut(s) 164
Eco57I CTGAAG 8 cut(s) 999, 1254, 1921, 2268, 3216, 3270, 3409, 3657
Eco81I CCTNAGG 1 cut(s) 281
Eco88I CYCGRG 6 cut(s) 85, 299, 591, 1203, 2039, 2212
EcoO109I RGGNCCY 8 cut(s) 923, 1178, 1307, 1361, 2771, 2813, 2897, 3574
EcoRI GAATTC 3 cut(s) 674, 1417, 2692
EcoRII CCWGG 3 cut(s) 2085, 2830, 2872
EcoT14I CCWWGG 6 cut(s) 577, 973, 1318, 1553, 1697, 3558
EcoT38I GRGCYC 3 cut(s) 481, 1574, 3246
ErhI CCWWGG 6 cut(s) 577, 973, 1318, 1553, 1697, 3558
Esp3I CGTCTC 1 cut(s) 835
FaqI GGGAC 5 cut(s) 1607, 2784, 2826, 2868, 3512
FauNDI CATATG 1 cut(s) 1987
FbaI TGATCA 5 cut(s) 391, 1519, 2350, 2365, 2536
FblI GTMKAC 1 cut(s) 2226
FriOI GRGCYC 3 cut(s) 481, 1574, 3246
GlaI GCGC 1 cut(s) 2642
GsuI CTGGAG 4 cut(s) 1407, 1473, 2496, 2928
HapII CCGG 6 cut(s) 109, 126, 2151, 2514, 3123, 3545
HgaI GACGC 4 cut(s) 193, 194, 852, 3358
HhaI GCGC 1 cut(s) 2643
Hin1I GRCGYC 1 cut(s) 76
Hin6I GCGC 1 cut(s) 2641
HinP1I GCGC 1 cut(s) 2641
HincII GTYRAC 4 cut(s) 1465, 2227, 2238, 2923
HindII GTYRAC 4 cut(s) 1465, 2227, 2238, 2923
HindIII AAGCTT 3 cut(s) 768, 876, 1131
HinfI GANTC 9 cut(s) 215, 259, 265, 609, 1733, 1895, 2803, 3148, 3254
HpaI GTTAAC 1 cut(s) 1465
HpaII CCGG 6 cut(s) 109, 126, 2151, 2514, 3123, 3545
Hpy99I CGWCG 2 cut(s) 64, 78
HpyCH4IV ACGT 4 cut(s) 76, 407, 1998, 3773
HpySE526I ACGT 4 cut(s) 76, 407, 1998, 3773
Hsp92I GRCGYC 1 cut(s) 76
HspAI GCGC 1 cut(s) 2641
Kpn2I TCCGGA 1 cut(s) 2513
Ksp22I TGATCA 5 cut(s) 391, 1519, 2350, 2365, 2536
KspAI GTTAAC 1 cut(s) 1465
LguI GCTCTTC 1 cut(s) 3078
LmnI GCTCC 4 cut(s) 1775, 2326, 2451, 2761
Lsp1109I GCAGC 9 cut(s) 909, 1130, 2152, 2272, 2281, 2284, 3217, 3518, 3528
LweI GCATC 9 cut(s) 555, 944, 1264, 2099, 2717, 2877, 3025, 3274, 3538
MaeII ACGT 4 cut(s) 76, 407, 1998, 3773
MbiI CCGCTC 2 cut(s) 169, 195
MfeI CAATTG 6 cut(s) 642, 987, 1242, 1425, 1792, 2415
MflI RGATCY 2 cut(s) 1490, 3895
MhlI GDGCHC 8 cut(s) 481, 1551, 1564, 1574, 3073, 3246, 3459, 3489
MlsI TGGCCA 3 cut(s) 933, 1317, 1500
MluNI TGGCCA 3 cut(s) 933, 1317, 1500
MlyI GAGTC 4 cut(s) 1904, 2797, 3142, 3248
MmeI TCCRAC 4 cut(s) 986, 1666, 1753, 1782
Mox20I TGGCCA 3 cut(s) 933, 1317, 1500
MroI TCCGGA 1 cut(s) 2513
MroXI GAANNNNTTC 5 cut(s) 696, 2253, 2280, 2994, 3801
MscI TGGCCA 3 cut(s) 933, 1317, 1500
MseI TTAA 9 cut(s) 372, 500, 1464, 3027, 3108, 3162, 3219, 3294, 3303
MslI CAYNNNNRTG 4 cut(s) 972, 1227, 3138, 3755
Msp20I TGGCCA 3 cut(s) 933, 1317, 1500
MspA1I CMGCKG 1 cut(s) 3116
MspI CCGG 6 cut(s) 109, 126, 2151, 2514, 3123, 3545
MspR9I CCNGG 4 cut(s) 127, 2087, 2832, 2874
MunI CAATTG 6 cut(s) 642, 987, 1242, 1425, 1792, 2415
Mva1269I GAATGC 3 cut(s) 1645, 2674, 2994
MvaI CCWGG 3 cut(s) 2087, 2832, 2874
MvnI CGCG 2 cut(s) 21, 54
NciI CCSGG 1 cut(s) 127
NcoI CCATGG 3 cut(s) 577, 973, 1318
NdeI CATATG 1 cut(s) 1987
NheI GCTAGC 1 cut(s) 494
NmuCI GTSAC 5 cut(s) 408, 596, 2374, 2428, 2467
NotI GCGGCCGC 1 cut(s) 164
NruI TCGCGA 2 cut(s) 21, 54
NspI RCATGY 3 cut(s) 604, 2756, 3422
PaeI GCATGC 1 cut(s) 3422
PaeR7I CTCGAG 4 cut(s) 591, 1203, 2039, 2212
PagI TCATGA 5 cut(s) 1516, 1786, 1813, 1879, 1960
PceI AGGCCT 2 cut(s) 1054, 2613
PciI ACATGT 1 cut(s) 2752
PciSI GCTCTTC 1 cut(s) 3078
PctI GAATGC 3 cut(s) 1645, 2674, 2994
PdmI GAANNNNTTC 5 cut(s) 696, 2253, 2280, 2994, 3801
PfeI GAWTC 5 cut(s) 215, 259, 265, 609, 1733
PflMI CCANNNNNTGG 1 cut(s) 3440
Ple19I CGATCG 1 cut(s) 52
PleI GAGTC 4 cut(s) 1903, 2797, 3142, 3248
PpsI GAGTC 4 cut(s) 1903, 2797, 3142, 3248
Ppu21I YACGTR 1 cut(s) 3774
PpuMI RGGWCCY 4 cut(s) 1307, 2771, 2813, 3574
PscI ACATGT 1 cut(s) 2752
PshAI GACNNNNGTC 2 cut(s) 204, 864
PsiI TTATAA 1 cut(s) 3020
Psp5II RGGWCCY 4 cut(s) 1307, 2771, 2813, 3574
Psp6I CCWGG 3 cut(s) 2085, 2830, 2872
PspGI CCWGG 3 cut(s) 2085, 2830, 2872
PspPPI RGGWCCY 4 cut(s) 1307, 2771, 2813, 3574
PspXI VCTCGAGB 3 cut(s) 591, 2039, 2212
PstNI CAGNNNCTG 2 cut(s) 3038, 3541
PsuI RGATCY 2 cut(s) 1490, 3895
PvuI CGATCG 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 3116
RruI TCGCGA 2 cut(s) 21, 54
RsaI GTAC 6 cut(s) 83, 137, 2001, 2205, 3681, 3722
RsaNI GTAC 6 cut(s) 82, 136, 2000, 2204, 3680, 3721
RseI CAYNNNNRTG 4 cut(s) 972, 1227, 3138, 3755
Rsr2I CGGWCCG 1 cut(s) 106
RsrII CGGWCCG 1 cut(s) 106
SalI GTCGAC 1 cut(s) 2225
SapI GCTCTTC 1 cut(s) 3078
SaqAI TTAA 9 cut(s) 372, 500, 1464, 3027, 3108, 3162, 3219, 3294, 3303
ScaI AGTACT 1 cut(s) 137
SchI GAGTC 4 cut(s) 1904, 2797, 3142, 3248
ScrFI CCNGG 4 cut(s) 127, 2087, 2832, 2874
SduI GDGCHC 8 cut(s) 481, 1551, 1564, 1574, 3073, 3246, 3459, 3489
SfaNI GCATC 9 cut(s) 555, 944, 1264, 2099, 2717, 2877, 3025, 3274, 3538
Sfr274I CTCGAG 4 cut(s) 591, 1203, 2039, 2212
SlaI CTCGAG 4 cut(s) 591, 1203, 2039, 2212
SmiMI CAYNNNNRTG 4 cut(s) 972, 1227, 3138, 3755
SmlI CTYRAG 6 cut(s) 591, 714, 1203, 2039, 2212, 3671
SmoI CTYRAG 6 cut(s) 591, 714, 1203, 2039, 2212, 3671
SnaBI TACGTA 1 cut(s) 3774
SphI GCATGC 1 cut(s) 3422
SseBI AGGCCT 2 cut(s) 1054, 2613
StuI AGGCCT 2 cut(s) 1054, 2613
StyD4I CCNGG 4 cut(s) 125, 2085, 2830, 2872
StyI CCWWGG 6 cut(s) 577, 973, 1318, 1553, 1697, 3558
TaiI ACGT 4 cut(s) 79, 410, 2001, 3776
TatI WGTACW 3 cut(s) 135, 2203, 3720
TauI GCSGC 6 cut(s) 32, 166, 169, 181, 198, 462
TfiI GAWTC 5 cut(s) 215, 259, 265, 609, 1733
Tru1I TTAA 9 cut(s) 372, 500, 1464, 3027, 3108, 3162, 3219, 3294, 3303
Tru9I TTAA 9 cut(s) 372, 500, 1464, 3027, 3108, 3162, 3219, 3294, 3303
TscAI CASTG 3 cut(s) 242, 2884, 3508
TseFI GTSAC 5 cut(s) 408, 596, 2374, 2428, 2467
TseI GCWGC 9 cut(s) 897, 1118, 2140, 2260, 2294, 2297, 3230, 3506, 3541
Tsp45I GTSAC 5 cut(s) 408, 596, 2374, 2428, 2467
TspGWI ACGGA 5 cut(s) 69, 77, 128, 1745, 3416
TspRI CASTG 3 cut(s) 242, 2884, 3508
Van91I CCANNNNNTGG 1 cut(s) 3440
VneI GTGCAC 1 cut(s) 1547
XapI RAATTY 5 cut(s) 674, 1417, 1886, 2692, 3306
XbaI TCTAGA 1 cut(s) 3898
XceI RCATGY 3 cut(s) 604, 2756, 3422
XcmI CCANNNNNNNNNTGG 1 cut(s) 1604
XhoI CTCGAG 4 cut(s) 591, 1203, 2039, 2212
XmaJI CCTAGG 1 cut(s) 1553
XmiI GTMKAC 1 cut(s) 2226
XmnI GAANNNNTTC 5 cut(s) 696, 2253, 2280, 2994, 3801
ZraI GACGTC 1 cut(s) 77
ZrmI AGTACT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.